HRP and AP Conjugates

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HRP and AP conjugates for western blotting

Overview

Conjugate Specifications
Products Volume Storage Condition Shelf Life Recommended Dilution
Avidin-HRP 2 ml 4°C 1 year 1:1000–1:3000
Goat Anti-Rabbit (H+L) 2 ml 4°C 1 year 1:3000
Goat Anti-Mouse (H+L) 2 ml 4°C 1 year 1:3000
Goat Anti-Human (H+L) 2 ml 4°C 1 year 1:3000
Protein A-HRP 1 ml 4°C 1 year 1:3000
Protein G-HRP 1 ml 4°C 1 year 1:3000
         
Goat Anti-Rabbit IgG-AP 1 ml 4°C 1 year 1:3000
Goat Anti-Mouse IgG-AP 1 ml 4°C 1 year 1:3000
Goat Anti-Human IgG-AP 1 ml 4°C 1 year 1:3000
Avidin-AP 1 ml 4°C 1 year 1:1000–1:3000
         
Biotinylated AP 1 ml 4°C 1 year 1:3000
Biotinylated GAR (H+L), human IgG adsorbed 1 ml 4°C 1 year 1:3000
Antibody Binding Affinities of Protein A and Protein G
Monoclonal Protein A Protein G
Human IgG1 +++ +++
Human IgG2 +++ +++
Human IgG3 +++
Human IgG4 +++ +++
Mouse IgG1 + +++
Mouse IgG2a +++ +++
Mouse IgG2b ++ ++
Mouse IgG3 ++ ++
Rat IgG1 +
Rat IgG2aa +++
Rat IgG2b ++
Rat IgG2c + ++
Polyclonal Protein A Protein G
Rabbit +++ ++
Cow ++ +++
Horse ++ +++
Goat ++
Guinea pig +++ ++
Sheep +/- ++
Pig ++ ++
Rat +/- ++
Mouse ++ ++
Chicken +
Human IgG +++ +++
Human IgM +
Human IgD +
Human IgA +

– No binding
+ Weak binding
++ Moderate binding
+++ Strong binding

Binding affinities of protein A and G to various species and subclasses of monoclonal and polyclonal antibodies.



Blotting-grade HRP and AP conjugates produce specific results, eliminating false positives in western blotting immunoassays

  • Double affinity-purified blotting-grade antibodies are isolated by affinity chromatography and further purified by cross-adsorption against an unrelated species to eliminate nonspecific immunoglobulins
  • The high titer of the blotting-grade antibody conjugates increase assay sensitivity. Greater working dilutions (1:3000) decreases background and increase the signal-to-noise ratio of the assay
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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
Goat Anti-Rabbit IgG (H + L)-HRP Conjugate

1706515
2 ml, blotting-grade horseradish peroxidase secondary antibody conjugate

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$190.00
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Goat Anti-Mouse IgG (H + L)-HRP Conjugate

1706516
2 ml, blotting-grade horseradish peroxidase secondary antibody conjugate

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$190.00
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Goat Anti-Human (H+L) HRP conjugate

1721050
2 ml, EIA-grade, enzyme-antibody conjugate

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$165.00
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Protein A-HRP Conjugate

1706522
1 ml, blotting-grade protein A horseradish peroxidase antibody conjugate

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$175.00
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Protein G-HRP Conjugate

1706425
1 ml, blotting-grade protein G horseradish peroxidase antibody conjugate

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$155.00
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Avidin-HRP

1706528
2 ml, HRP-Avidin conjugate for detection of biotinylated SDS-PAGE standards

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$120.00
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Immun-Star Goat Anti-Mouse (GAM)-HRP Conjugate

1705047
2 ml, horseradish peroxidase secondary antibody conjugate, for use with Immun-Star HRP chemiluminescent detection kits

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$220.00
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Immun-Star Goat Anti-Rabbit (GAR)-HRP Conjugate

1705046
2 ml, horseradish peroxidase secondary antibody conjugate, for use with Immun-Star HRP chemiluminescent detection kits

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$220.00
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Goat Anti-Rabbit IgG-AP Conjugate

1706518
1 ml, blotting-grade alkaline phosphatase secondary antibody conjugate

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$195.00
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Goat Anti-Mouse IgG-AP Conjugate

1706520
1 ml, blotting-grade alkaline phosphatase secondary antibody conjugate

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$195.00
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Goat Anti-Human IgG-AP Conjugate

1706521
1 ml, blotting-grade alkaline phosphatase secondary antibody conjugate

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$190.00
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Avidin-AP

1706533
1 ml, blotting-grade alkaline phosphatase (AP) avidin conjugate

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$130.00
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Blotting-Grade Biotinylated AP Conjugate

1706403
1 ml, alkaline phosphatase (AP) secondary antibody conjugate

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$175.00
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Number Description Options
Lit423 Instruction Manual, Blotting-Grade Avidin-Horseradish Peroxidase Conjugate, Rev E Click to download
5576 Molecular Weight Estimation and Quantitation of Protein Samples Using Precision Plus Protein WesternC Standards, the Immun-Star WesternC Chemiluminescent Detection Kit, and the Molecular Imager ChemiDoc XRS Imaging System, Rev C Click to download
Lit417 Instruction Manual, Blotting Grade Protein G - Horseradish Peroxidate Conjugate, Rev C Click to download
LIT422 Instruction Manual, Blotting Grade Protein A- Horseradish Peroxidase Conjugate Click to download
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LIT372 Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Mouse IgG (H + L) Horseradish Peroxidase Conjugate, Rev D Click to download
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LIT421 Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Human IgG (H + L) Alkaline Phosphatase Conjugate Click to download
LIT440 Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Human IgG ((H + L) Horseradish Peroxidase Conjugate Click to download
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download