High Resolution Melt Analysis

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Precision Melt Supermix

Overview

Robust HRM for Class I–IV SNPs
Robust HRM

Precision melt supermix delivers robust HRM for class I–IV SNPs. Discrimination of A, class I (A to G substitution); B, class II (G to T substitution); C, class III (G to C substitution); and D, class IV (A to T substitution) SNP genotypes from mouse genomic DNA using precision melt supermix. Wild type (), heterozygote (), and homozygous mutant () are shown in the difference plots normalized to wild-type samples. HRM analysis was performed on a CFX384™ real-time PCR detection system and genotypes were automatically assigned by Precision Melt Analysis™ software. Amplification was carried out for 35 cycles. Total run time including melt curve = 150 min. RFU, relative fluorescence units.

Accurate Methylation Detection
Accurate Methylation Detection

Accurate methylation detection with precision melt supermix. A, normalized melt curve; B, difference curve. Mixtures of methylated and unmethylated human genomic DNA of varying ratios were analyzed using HRM on a CFX384™ real-time PCR detection system. Increasing amounts of methylated DNA (, 0%; , 2%; , 5%; , 50%; , 75%; , 95%; and , 100%) were analyzed for methylation of the human RARB2 gene. The genomic region contains 7 CpG sites and is 88 base pairs in length. Total run time including melt curve = 190 min. RFU, relative fluorescence units.

Exceptional Stability
Exceptional Stability

Exceptional stability enables high-throughput genotyping analysis with precision melt supermix. Specific amplification and accurate discrimination of a class IV SNP (84 bp amplicon) from mouse genomic DNA was performed on a CFX384™ real-time PCR detection system either 0 hr (A) or 48 hr (B) after reaction setup. Wild type (), heterozygote (), and homozgous mutant () are shown in the difference plots normalized to wild-type samples. Total run time including melt curve = 150 min. RFU, relative fluorescence units.

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Precision Melt Supermix is a superior high-resolution melt (HRM) supermix delivering optimal discrimination for numerous applications including:

  • Genotyping
  • Mutation analyses
  • Methylation studies
  • Species identification
  • Epigenetic analyses
  • De novo SNP discovery

Key Features, Benefits and Applications of Precision Melt Supermix

  • Sensitive and specific discrimination of class I–IV single nucleotide polymorphisms (SNPs) across a broad range of amplicons
  • Ideal solution for insertion/deletions >6 base pairs
  • Accurate detection of the percent of CpG methylation status for epigenetic studies
  • Ideal for mutation screening of small mutations or using a primer walking approach for larger regions
  • Ideal cost-saving application for upstream and downstream next generation sequencing pre-screens and post-validations
  • Exceptional room temperature stability for high-throughput HRM studies
  • Optimized formulation containing EvaGreen dye delivers robust PCR and HRM performance

Instrument Compatibility

For use with all HRM-ready real-time PCR systems; dye calibration may be required.

Packaging Options

Number of 20 µl Reactions
Volume Catalog Number
200 2 ml 1725110
1,000 10 ml
1725112

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Storage at –20°C  Guaranteed for 6 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)
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Precision Melt Supermix for High Resolution Melt (HRM) Analysis, 200 x 20 µl reactions, 2 x 1 ml

1725110
2 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, EvaGreen dye, stabilizers, for 200 x 20 µl reactions

1725110 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5110_1309291718.xml 1725110 06/28/11 01:06 PM en_US true internet/product_SKU 2018-03-06 01:08:39 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5110_1309291718.xml /default/main/bio-rad/WORKAREA/default jcrucypy 2017-11-14 07:07:36 true jcrucypy templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5110_1309291718.xml LSR N /default/main/bio-rad en 06/28/23 01:06 PM 1520037978060 2018-03-06 01:08:39 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5110_1309291718.xml 1725110-precision-melt-supermix-for-high-resolution-melt-hrm-analysis-200-x-20-ul-reactions-2-x-1-ml 1 1725110 Precision Melt Supermix Precision Melt Supermix for High Resolution Melt (HRM) Analysis, 200 x 20 µl reactions, 2 x 1 ml 2 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq&trade; DNA polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 200 x 20 &micro;l reactions 2 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq&trade; DNA polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 200 x 20 &micro;l reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5110_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5110_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/172-5110_feature.jpg Karen Moss Precision Melt Real-Time PCR Supermix, for High Resolution Melt (HRM) Analysis 2 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq&trade; DNA polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 200 x 20 &micro;l reactions 1725110, precision, melt, supermix, real-time, real, time, pcr, qpcr, mix, detection, amplification, dntps, itaq, dna, polymerase, mgcl2, evagreen, dye, stabilizers 06/28/11 01:06 PM 06/28/23 01:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p><strong>Packaging Options</strong></p> <ul> <li><strong>Catalog #</strong><strong>172-5110</strong>, 2 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq&trade; DNA polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 200 x 20 &micro;l reactions</li> <li><strong>Catalog #</strong><a href="/evportal/destination/commerce/sku_detail?productID=172-5112"><strong>172-5112</strong></a>, 10 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq&trade; DNA polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 1,000 x 20 &micro;l reactions</li> </ul> <p>Bio-Rad introduces precision melt supermix, a high-performance supermix, to complement its line of products for high resolution melt (HRM) analysis, delivering superior discrimination for both genotyping and epigenetic analyses.</p> <p><strong>Key Features and Benefits</strong></p> <ul> <li>Optimized formulation containing EvaGreen dye delivers robust PCR and HRM performance</li> <li>Sensitive and effective discrimination of class I&ndash;IV single nucleotide polymorphisms (SNPs) across a broad range of amplicons</li> <li>Accurate detection of CpG methylation status for epigenetic studies</li> <li>Exceptional room temperature stability for high-throughput HRM studies</li> <li>Reliable performance on any HRM-capable thermal cycler</li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global 172-5110_desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/172-5110_desc.html &nbsp; 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725110&CartQuote=QT&quantity= false true false $170.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725110&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725110&quantity= 1725110
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Precision Melt Supermix for High Resolution Melt (HRM) Analysis, 1,000 x 20 µl reactions, 10 x 1 ml

1725112
10 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, EvaGreen dye, stabilizers, for 1,000 x 20 µl reactions

1725112 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5112_1309290808.xml 1725112 06/28/11 12:42 PM en_US true internet/product_SKU 2018-03-06 01:08:39 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5112_1309290808.xml /default/main/bio-rad/WORKAREA/default jcrucysk 2017-11-14 07:07:36 true jcrucysk templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5112_1309290808.xml LSR N /default/main/bio-rad en 06/28/23 12:42 PM 1520037978103 2018-03-06 01:08:39 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5112_1309290808.xml 1725112-precision-melt-supermix-for-high-resolution-melt-hrm-analysis-1000-x-20-ul-reactions-10-x-1-ml 1 1725112 Precision Melt Supermix Precision Melt Supermix for High Resolution Melt (HRM) Analysis, 1,000 x 20 µl reactions, 10 x 1 ml 10 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq&trade; DNA polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 1,000 x 20 &micro;l reactions 10 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq&trade; DNA polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 1,000 x 20 &micro;l reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/172-5112_tn.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/172-5112_view.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_feature/global/172-5112_feature.jpg Karen Moss Precision Melt Real-Time PCR Supermix, for High Resolution Melt (HRM) Analysis 10 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq&trade; DNA polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 1,000 x 20 &micro;l reactions 1725112, precision, melt, supermix, real-time, pcr, mix, master, mastermix, real, time, qpcr, detection, amplification 06/28/11 12:42 PM 06/28/23 12:42 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p><strong>Packaging Options</strong></p> <ul> <li><strong>Catalog #<a href="/evportal/destination/commerce/sku_detail?productID=172-5110"><strong>172-5110</strong></a></strong>, 2 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq&trade; DNA polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 200 x 20 &micro;l reactions</li> <li><strong>Catalog #</strong><strong>172-5112</strong>, 10 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq&trade; DNA polymerase, MgCl<sub>2</sub>, EvaGreen dye, stabilizers, for 1,000 x 20 &micro;l reactions</li> </ul> <p>Bio-Rad introduces precision melt supermix, a high-performance supermix, to complement its line of products for high resolution melt (HRM) analysis, delivering superior discrimination for both genotyping and epigenetic analyses.</p> <p><strong>Key Features and Benefits</strong></p> <ul> <li>Optimized formulation containing EvaGreen dye delivers robust PCR and HRM performance</li> <li>Sensitive and effective discrimination of class I&ndash;IV single nucleotide polymorphisms (SNPs) across a broad range of amplicons</li> <li>Accurate detection of CpG methylation status for epigenetic studies</li> <li>Exceptional room temperature stability for high-throughput HRM studies</li> <li>Reliable performance on any HRM-capable thermal cycler</li> </ul> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global 172-5112_desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/172-5112_desc.html &nbsp; 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725112&CartQuote=QT&quantity= false true false $721.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725112&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725112&quantity= 1725112
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Number Description Options
10022094 Instruction Manual, Precision Melt Supermix, Rev B Click to download
6137 Precision Melt Supermix Product Information Sheet, Rev A Click to download
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
6773 Real-Time PCR Supermixes Flier, Ver A Click to download

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