hFAB™ Rhodamine Housekeeping Protein Fluorescent Primary Antibodies

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hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies

Overview

Triplex Blot Example
Triplex blot image
Simultaneous detection of multiple proteins (ATG7, AKR1C2, and tubulin) on a single blot using fluorescent antibodies.
Target Protein Color 1º Antibody 2º Antibody Exposure Time (seconds)
ATG7 Blue ATG7 Rabbit Anti-Human
(78 kDa)
StarBright™ Blue 700 Goat Anti-Rabbit 6.9
AKR1C2 Red AKR1C2 Mouse Anti-Human (36 kDa) Goat Anti-Mouse IgG DyLight 800 300.0
Tubulin Green hFAB™ Rhodamine Anti-Tubulin
(50 kDa)
141.9




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hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies allow one-step detection of common housekeeping proteins like actin, tubulin, and GAPDH in your samples. These anti-actin, anti-tubulin, and anti-GAPDH antibodies are directly labeled with a rhodamine derivative (Exmax/Emmax = 530 nm/580 nm). The hFAB Rhodamine Antibodies were generated with Human Combinatorial Antibody Library (HuCAL®) Technology, providing exceptional specificity.

These housekeeping protein antibodies can be used for protein normalization when the protein loading amounts are in the linear dynamic range. hFAB Rhodamine Antibodies can be imaged with the ChemiDoc™ MP Imaging System, and can be used in multiplex experiments with StarBright™ Blue 700 Secondary Antibodies as well as other traditional fluorescent antibodies, permitting detection of multiple target proteins and the housekeeping protein in a single blot.

Features and Benefits

  • Simple — No requirement for a secondary antibody; one-step detection of housekeeping proteins using highly cross-adsorbed antibodies for high specificity and sensitivity
  • Versatile — Compatible with use of a wide range of other fluorophores including StarBright Blue 700 and DyLight 800 Fluorophores for multiplex western blotting
  • Unique — HuCAL Technology eliminates cross-reactivity with primary/secondary antibodies from other species
  • Diverse — Antibodies have been tested to detect human or mouse housekeeping proteins

Related Products

Related Categories

Supporting Documents

  • hFAB Rhodamine Housekeeping Antibodies Instruction Manual
  • Fluorescent Western Blotting with StarBright Blue 700 Secondary Antibodies Selection Guide
  • StarBright Blue 700 Secondary Antibodies Flier

Online Resources

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Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

hFAB™ Rhodamine Anti-Actin Antibody, 40 µl
hFAB™ Rhodamine Anti-Actin Primary Antibody, 40 µl

12004164
Pkg of 1, lyophilized rhodamine-conjugated anti-actin human Fab fragment; reconstitute to 40 µl

List Price:
 
$100.00
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hFAB™ Rhodamine Anti-Actin Antibody, 200 µl
hFAB™ Rhodamine Anti-Actin Primary Antibody, 200 µl

12004163
Pkg of 1, lyophilized rhodamine-conjugated anti-actin human Fab fragment; reconstitute to 200 µl

List Price:
 
$300.00
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hFAB™ Rhodamine Anti-Tubulin Antibody 40 µl
hFAB™ Rhodamine Anti-Tubulin Primary Antibody 40 µl

12004166
Pkg of 1, lyophilized rhodamine-conjugated anti-tubulin human Fab fragment; reconstitute to 40 µl

List Price:
 
$100.00
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hFAB™ Rhodamine Anti-Tubulin Antibody, 200 µl
hFAB™ Rhodamine Anti-Tubulin Primary Antibody, 200 µl

12004165
Pkg of 1, lyophilized rhodamine-conjugated anti-tubulin human Fab fragment; reconstitute to 200 µl

List Price:
 
$300.00
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hFAB™ Rhodamine Anti-GAPDH 40 µl
hFAB™ Rhodamine Anti-GAPDH Primary Antibody, 40 µl

12004168
Pkg of 1, lyophilized rhodamine-conjugated anti-GAPDH human Fab fragment; reconstitute to 40 µl

List Price:
 
$100.00
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hFAB™ Rhodamine Anti-GAPDH Antibody, 200 µl
hFAB™ Rhodamine Anti-GAPDH Primary Antibody, 200 µl

12004167
Pkg of 1, lyophilized rhodamine-conjugated anti-GAPDH human Fab fragment; reconstitute to 200 µl

List Price:
 
$300.00
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Number Description Options
10000068702 hFAB™ Rhodamine Housekeeping Antibodies Instruction Manual, Ver B Click to download
10000078050 Instruction Manual, StarBright™ Blue 700 Secondary Antibodies and hFAB™ Rhodamine Anti‑Housekeeping Protein Primary Antibodies Click to download
10000068973 Fluorescent Western Blotting with StarBright™ Blue 700 Secondary Antibodies Selection Guide, Ver A Click to download
6911 StarBright™ Blue 700 Secondary Antibodies Flier Click to download