Hepatitis B Serology

Serology Profiles

Hepatitis B: Example of Acute Hepatitis

Hepatitis Testing

Comprehensive Product Line for Hepatitis Testing

Fast incubation time:
2.5 hours
  Standardized procedure
for all Bio-Rad assays
  Microplate strip identification
Common reagents for
all Bio-Rad kits
  Color-coded reagent
and kit labels
  Color changes during
protocol steps
Manual and automated use   Barcoded reagents   Controls with
color-coded caps
Hepatitis Testing

MONOLISA™ Anti-HBc EIA Procedure

Test Format

  Color of well
BEFORE addition
  Color of well
AFTER addition
  • Microplate coated with recombinant HBc antigen

  • 200 μL of Specimen Diluent
  • 20 μL of control or sample

  • Incubate for 60 minutes at 37°C
  • 100 μL of Working Conjugate Solution

  • Incubate for 60 minutes at 37°C
  • 100 μL of Working TMB Solution

  • Incubation for 30 minutes at room temperature (18–30°C) in the dark
  • 100 μL of Stopping Solution

  • Cutoff = Calibrator
    Cutoff Mean + 0.250
READ at dual wavelength 615/630 nm
HBcAg Recombinant Protein   Anti-HBc Ab   Anti-IgG/IgM Conjugate   * Reactive samples after 30 minute incubation

MONOLISA™ Anti-HBc EIA is for the detection of total antibodies to hepatitis B core antigen (HBc) in human serum or plasma. The MONOLISA™ Anti-HBc EIA is intended for manual use and for use with the Bio-Rad EVOLIS™ Automated Microplate System in the detection of total antibodies to hepatitis B core antigen.

Bio-Rad offers a full range of sensitive and specific hepatitis B microplate assays to detect every stage of hepatitis B virus (HBV) infection. All Bio-Rad hepatitis B enzyme immunoassays (EIA) feature common reagents and procedures for use with other Bio-Rad assays, fast incubation time (2.5 hours), and easy automation. Advanced safety features include: barcoded reagents, color-coded reagents and reagent labels, microplate strip identification, color monitoring for sample and conjugate addition steps, and color changes during protocol steps.


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192 tests for detection of total antibodies to hepatitis B nucleocapsid core antigen in human serum or plasma

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P-130 Hepatitis Product Brochure (U.S. Version) Click to download [ Add to Cart (Free) ]