Hepatitis B Serology

Serology Profiles

Hepatitis B: Example of Acute Hepatitis

Hepatitis Testing

Comprehensive Product Line for Hepatitis Testing

Fast incubation time:
2.5 hours
  Standardized procedure
for all Bio-Rad assays
  Microplate strip identification
Common reagents for
all Bio-Rad kits
  Color-coded reagent
and kit labels
  Color changes during
protocol steps
Manual and automated use   Barcoded reagents   Controls with
color-coded caps
Hepatitis Testing

MONOLISA™ Anti-HBc EIA Procedure

Test Format

  Color of well
BEFORE addition
  Color of well
AFTER addition
  • Microplate coated with recombinant HBc antigen

  • 200 μL of Specimen Diluent
  • 20 μL of control or sample

  • Incubate for 60 minutes at 37°C
  • 100 μL of Working Conjugate Solution

  • Incubate for 60 minutes at 37°C
  • 100 μL of Working TMB Solution

  • Incubation for 30 minutes at room temperature (18–30°C) in the dark
  • 100 μL of Stopping Solution

  • Cutoff = Calibrator
    Cutoff Mean + 0.250
READ at dual wavelength 615/630 nm
HBcAg Recombinant Protein   Anti-HBc Ab   Anti-IgG/IgM Conjugate   * Reactive samples after 30 minute incubation

MONOLISA™ Anti-HBc EIA is for the detection of total antibodies to hepatitis B core antigen (HBc) in human serum or plasma. The MONOLISA™ Anti-HBc EIA is intended for manual use and for use with the Bio-Rad EVOLIS™ Automated Microplate System in the detection of total antibodies to hepatitis B core antigen.

Bio-Rad offers a full range of sensitive and specific hepatitis B microplate assays to detect every stage of hepatitis B virus (HBV) infection. All Bio-Rad hepatitis B enzyme immunoassays (EIA) feature common reagents and procedures for use with other Bio-Rad assays, fast incubation time (2.5 hours), and easy automation. Advanced safety features include: barcoded reagents, color-coded reagents and reagent labels, microplate strip identification, color monitoring for sample and conjugate addition steps, and color changes during protocol steps.


192 tests for detection of total antibodies to hepatitis B nucleocapsid core antigen in human serum or plasma

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P-130 Hepatitis Product Brochure (U.S. Version) Click to download [ Add to Cart (Free) ]