GS HBsAg EIA 3.0

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Overview

GS HBsAg EIA 3.0

Earliest Hepatitis B Infection Detection Demonstrated on
21 Commercial Seroconversion Panels*

GS HBsAg EIA 3.0 Reactivity on Comercial HBsAg
Seroconversion Panels Versus Competitors
(Earliest Detection Indicated in Bold Type)
Panel ID#
GS HBsAg EIA 3.0
HBsAg EIA
Competitor #1

(overnight)
HBsAg EIA
Competitor #2
Static
Shaker
902
7
7
9
9
903
3
2
5
5
904
2
1
3
3
905
1
1
1
1
906
2
2
2
3
907
6
5
6
6
908
6
4
7
7
909
4
3
4
5
910
3
3
3
4
911
20
20
21
22
912
7
6
8
8
914
2
2
5
5
915
1
1
9
11
917
2
2
3
3
918
2
2
3
3
919
5
4
6
7
920
3
3
3
3
924
3
2
3
4
931
5
3
NT
7
932
10
8
NT
10
40565L
2
1
3
NT


* The sensitivity of GS HBsAg EIA 3.0, procedures A and B, was assessed using 20 seroconversion panels purchased from Boston Biomedica, Inc. (West Bridgewater, MA), and 1 panel from Serologicals, Inc. (Clarkston, GA). The number of the bleed at which HBsAg was detected (S/C ≥ 1.0) by the GS HBsAg EIA 3.0, procedures A and B, is compared to other licensed HBsAg assays (as recorded in the certificates of analysis).


GS HBsAg 3.0 Static Procedure

  • Earlier detection on 12 out of 19 seroconversions for HBsAg 3.0 versus competitor assay number 1
  • Earlier detection on 16 out of 20 seroconversions for HBsAg 3.0 versus competitor assay number 2

GS HBsAg 3.0 Shaking Procedure

  • Earlier detection on 15 out of 19 seroconversions versus competitor assay number 1
  • Earlier detection on 18 out of 20 seroconversions versus competitor assay number 2
GS HBsAg EIA 3.0

GS HBsAg EIA 3.0 Testing Procedure

Test Format +   Sample/Reagent Addition Monitoring
  • Microplate coated with monoclonal antibody (Ab)
Color of well
BEFORE addition
  Color of well
AFTER addition
  • 100 µL of control or sample
  • Incubate 60–65 minutes at 37°C
Wash
  • 100 µL of Working Conjugate Solution
  • Incubate 60–65 minutes at 37°C
 
Wash
  • 100 µL of Working TMB Solution
  • Incubate 30 minutes at
    room temperature
  • 100 µL of Stopping Solution
 
READ within 30 minutes at 450 nm with the 615–630 nm filter as a reference
Anti-HBsAg
Monoclonal Ab
  HBsAg   Anti-HBsAg
Monoclonal Ab Conjugate

+

*

Static or Shaker

Reactive samples after 30 minute incubation

Hepatitis B Serology

Serology Profiles

Hepatitis B: Example of Acute Hepatitis

Hepatitis Testing

Comprehensive Product Line for Hepatitis Testing

   
Fast incubation time:
2.5 hours
  Standardized procedure
for all Bio-Rad assays
  Microplate strip identification
   
Common reagents for
all Bio-Rad kits
  Color-coded reagent
and kit labels
  Color changes during
protocol steps
   
Manual and automated use   Barcoded reagents   Controls with
color-coded caps

GS HBsAg 3.0 is an enzyme immunoassay (EIA) intended for the detection of Hepatitis B surface antigen (HBsAg) in human serum, plasma and cadaveric serum specimens. Bio-Rad offers a full range of sensitive and specific Hepatitis B Microplate assays to detect every stage of Hepatitis B virus (HBV) infection. All Bio-Rad Hepatitis B enzyme immunoassays (EIA) feature common reagents and procedures for use with other Bio-Rad assays, fast incubation time (2.5 hours) and easy automation. Advanced safety features include: bar-coded reagents, color-coded reagents and reagent labels, microplate strip identification, color monitoring for sample and conjugate addition steps and color changes during protocol steps. A range of ordering options is available to suit unique laboratory needs and test volumes.

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GS HBsAg EIA 3.0
GS HBsAg EIA 3.0

32591
480 tests for detection of hepatitis B surface antigen (HBsAg) in human serum, plasma, and cadaveric serum specimens

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GS HBsAg EIA 3.0
GS HBsAg EIA 3.0

25258
4800 tests for detection of hepatitis B surface antigen (HBsAg) in human serum, plasma, and cadaveric serum specimens

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