Helios® Gene Gun System

Helios® Gene Gun System

The Helios Gene Gun is a convenient handheld device that provides direct, rapid, and versatile gene delivery into a range of targets in vivo.

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Overview

The Helios Gene Gun is a convenient handheld device that provides rapid and direct transfection into a range of targets in vivo. The unit uses an adjustable low-pressure helium pulse to sweep DNA-, RNA-, or biomaterial-coated gold microcarriers from the inner wall of a small plastic cartridge directly into target cells.

Key Features

  • Provides easy-to-use, rapid, versatile gene delivery independent of target cell type
  • Facilitates both transient and stable expression
  • Requires only small amounts of DNA and cells; no carrier DNA needed
  • Enables codelivery of more than one plasmid
  • Allows transfer of large DNA fragments
  • Targets intracellular gene delivery to many cells
  • Works for both in vitro and in vivo transformation
  • Delivers no extraneous genes or proteins

 

 

Helios® Gene Gun Training Video.

This video provides an overview of the Helios Gene Gun Biolistic Particle Transfection System, including instrument setup, microcarrier and cartridge preparation, and instrument operation.

Specifications

Helios Gene Gun System
Functional
Maximum current
10 mA peak
Input voltage
9 V alkaline battery, replaceable
Battery life
1,000 discharges in continuous use
Gas pressure
600 psi maximum helium
Safety relief pressure
700 ± 35 psi at regulator assembly
Regulator adjustment
800 psi limit maximum
Discharges
12 per cylinder, mechanical indexing
Environmental
Operating temperature
10–32°C (50–90°F); 30–80% humidity
Storage temperature
0–60°C (32–110°F); 10–90% humidity
Physical
Dimensions (W x D x H) 
20 x 25 cm (8 x 10"; approximate)
Weight 
1.43 kg (3.15 lb)

Use

For Research Use Only. Not for use in diagnostic procedures.
Tubing Prep Station
Electrical
Maximum current
62 mA/125 mA peak
Input voltage
220/240 V or 100/120 V
Functional
Input frequency
50/60 Hz
Safety relief pressure
30 ± 1.5 psi at regulator assembly
Speed
30 rpm nominal
Tubing fill
Manual
Physical
Construction
Aluminium and acrylic
Dimensions (W x D x H) 
85 x 10 cm (33.5 x 4"; approximate)
Weight 
5.08 kg (11.2 lb)

Ordering

items

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(Discontinued) Helios Gene Gun Kit
1652411
100/120 V, handheld biolistic system, includes gene gun, 5 O-rings, 5 barrel liners, 5 white cartridge holders, cartridge extractor tool, 9 V battery.
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1652431

100/120 V, handheld biolistic system, includes helium hose assembly with regulator, tubing prep station (#1652418), syringe kit, Tefzel tubing, tubing cutter, optimization kit (#1652424)

Accessories

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(Discontinued) Helium Hose Assembly
1652412
Pkg of 1, Helios gene gun accessory, connects to helium source for safety and consistent helium delivery, with Swagelok quick-connect fittings
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(Discontinued) Helium Regulator
1652413
Pkg of 1, pressure regulator for use with Helios gene gun, CGA 580 female fitting (U.S. standard) with pressure relief valve, max. pressure 2,600 psi
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1652414
Pkg of 1, pressure regulator for use with Helios gene gun, CGA 580 female fitting (U.S. standard) with pressure relief valve, max. pressure 400 psi
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(Discontinued) Syringe Kit
1652421
Kit for loading gold suspension into Tefzel tubing, includes syringe adaptor tubing, 5 x 10 ml syringes, syringe adaptor fitting, 5 Luer-Lok fittings
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1652424
Optimization kit for the Helios gene gun, includes 0.6 μm, 1.0 μm and 1.6 μm gold microcarriers, PVP, desiccant, 5 cartridge storage vials, 50 feet of Tefzel tubing, for 1,000 samples
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(Discontinued) Cartridge Kit
1652440
Kit for preparing Helios gene gun cartridges, includes 0.5 g PVP, 5 desiccant pellets, 5 cartridge collection/storage vials, 15 m of Tefzel tubing
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1652425
Pkg of 1, nitrogen regulator, for drying Tefzel tubing during Helios gene gun sample preparation
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(Discontinued) Battery
1652436
Pkg of 1, 9 V alkaline battery, for use with the Helios gene gun, provides 1,000 discharges in continuous use
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(Discontinued) Tefzel Tubing
1652441
15 m, tubing for coating with DNA- or RNA-gold microcarrier complexes, used with the Helios® gene gun, .125" (3.175 mm) OD, .093" (2.36 mm) ID

Microcarriers

Documents

Number Description Options
5443
Biolistic Particle Delivery Systems Brochure, Rev A
5446
Helios Gene Gun System Flier, Rev A
10000118158
Instruction Manual, Helios Gene Gun System
10000148591
Update to EN 61010-1 Amendment Statement of Operating Conditions
4106137
Instruction Manual, GeneShot Control Cartridges
M1652475
Instruction Manual, Helios® Gene Gun Diffusion Screen, Rev A
2552
The Gene Gun: Current Applications in Cutaneous Gene Therapy, Rev A
2433
Transformation of Nematodes With the Helios Gene Gun
2453
Optimization of Gene Delivery Into Arabidopsis, Tobacco, and Birch Using the Helios Gene Gun System
2531
Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun
2726
Delivery of pCMV-S DNA Using the Helios® Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice
1689
Comparison of Performance Characteristics of Different Biolistic® Devices
2768
Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
2410
Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios® Gene Gun-Mediated Particle Bombardment, Rev B