Helios® Gene Gun System

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Overview

Specifications
Helios Gene Gun System   Tubing Prep Station
Functional   Electrical
Maximum current 10 mA peak   Maximum current 62 mA/125 mA peak
Voltage input 9 V alkaline battery, replaceable   Voltage input 220/240 V or 100/120 V
Battery life 1,000 discharges in continuous use   Functional
Gas pressure 600 psi maximum helium   Input frequency 50/60 Hz
Safety relief pressure 700 ± 35 psi at regulator assembly   Safety relief pressure 30 ± 1.5 psi at regulator assembly
Regulator adjustment 800 psi limit maximum   Speed 30 rpm nominal
Discharges 12 per cylinder, mechanical indexing   Tubing fill Manual
Environmental   Physical
Operating temperature 10–32°C (50–90°F); 30–80% humidity   Construction Aluminium and acrylic
Storage temperature 0–60°C (32–110°F); 10–90% humidity   Dimensions
(W x D x H)
85 x 10 cm (33.5 x 4"; approximate)
Physical   Weight 5.08 kg (11.2 lb)
Dimensions
(W x D x H)
20 x 25 cm (8 x 10"; approximate)    
Weight 1.42 kg (3.13 lb)    
Sample Delivery

Schematic of sample delivery using the Helios gene gun.


Biolistic Applications
Transformation Factors Helios Gene Gun System PDS-1000/He System
Experimental conditions In situ, in vitro
In vivo, ex vivo
In vitro, ex vivo
in vivo (plants)
Target area Small (2 cm2) Large (40 cm2)
Pressure range 100–600 psi 450–2,200 psi
Target type Animals: Any tissue exposed to barrel (skin, organs); cell, explant, and organ culture
Plants: Field and greenhouse use, plant cell culture, explants
Yeast, bacteria, other microbes
Animals: Cell and organ culture
Plants: Small intact plants, plant cell culture, explants
Yeast, bacteria, other microbes
Organelles (chloroplasts, mitochondria, etc.)
Transfection Protocol Library


The Transfection Protocol Online Library contains protocols obtained from the literature, developed by Bio-Rad scientists, or submitted by scientists like you. browse protocols to view our library and find your starting point.

Bio-Rad Laboratories has helped scientists deliver molecules into cells for over 35 years. Instruments, including the Gene Pulser® series of electroporators, the MicroPulser electroporator, and the Helios® gene gun and PDS-1000/He biolistic systems, have been used successfully to transfect prokaryotic and eukaryotic cells, including plant and animal cells. These transfection instruments are cited extensively in the literature. Our chemically mediated methods include lipid-based transfection reagents — TransFectin lipid reagent for delivery of plasmid DNA and siLentFect lipid reagent for siRNA delivery.

The Helios gene gun is a convenient handheld device that provides rapid and direct transfection into a range of targets in vivo. The unit uses an adjustable low-pressure helium pulse to sweep DNA-, RNA-, or biomaterial-coated gold microcarriers from the inner wall of a small plastic cartridge directly into target cells.

Key Features

  • Provides easy-to-use, rapid, versatile gene delivery independent of target cell type
  • Facilitates both transient and stable expression
  • Requires only small amounts of DNA and cells; no carrier DNA needed
  • Enables codelivery of more than one plasmid
  • Allows transfer of large DNA fragments
  • Targets intracellular gene delivery to many cells
  • Works for both in vitro and in vivo transformation
  • Delivers no extraneous genes or proteins
Helios Gene Gun Kit

165-2411
100/120 V, handheld biolistic system, includes gene gun, 5 O-rings, 5 barrel liners, 5 white cartridge holders, cartridge extractor tool, 9 V battery.

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Helios Gene Gun System

165-2431
100/120 V, handheld biolistic system, includes helium hose assembly with regulator, tubing prep station (#165-2418), syringe kit, Tefzel tubing, tubing cutter, optimization kit (#165-2424)

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Helios Gene Gun Low-Pressure System

165-2451
100/120 V, handheld biolistic system, includes helium hose assembly with low-pressure regulator, tubing prep station (#165-2418), syringe kit, tubing cutter, optimization kit (#165-2424)

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Number Description Options
5443 Biolistic Particle Delivery Systems Brochure, Rev A Click to download
5446 Helios Gene Gun System Flier, Rev A Click to download
M1652411 Instruction Manual, Helios Gene Gun Click to download
4106137 Instruction Manual, GeneShot Control Cartridges Click to download
M1652475 Instruction Manual, Helios Gene Gun Diffusion Screen, Rev A Click to download
2552 The Gene Gun: Current Applications in Cutaneous Gene Therapy, Rev A Click to download
2433 Transformation of Nematodes With the Helios Gene Gun Click to download
2453 Optimization of Gene Delivery Into Arabidopsis, Tobacco, and Birch Using the Helios Gene Gun System Click to download
2531 Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun Click to download
2726 Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice Click to download
1689 Comparison of Performance Characteristics of Different Biolistic Devices Click to download
2768 Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A Click to download
2410 Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios® Gene Gun-Mediated Particle Bombardment, Rev B Click to download
2497 Biolistic Purchase Demonstration Agreement, Rev E Click to download