Hard-Shell® 384-Well 480 PCR Plates

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Overview

Hard-Shell® PCR Plate Compatibility Chart

Hard-Shell PCR plates represent an innovative concept in PCR plate design. The skirt and deck are molded from a rigid thermostable polymer. This hard shell prevents the distortion and shrinkage that may occur when regular single-component polypropylene PCR plates are exposed to the high temperatures of thermal cycling or heat sealing. Bio-Rad offers a variety of Hard-Shell 96-well and 384-well PCR plates.

Hard-Shell 96-Well PCR Plates

 
High-Profile
96-Well
Semi-Skirted Plates

Low-Profile
96-Well Skirted Plates

Low-Profile
96-Well
Semi-Skirted Plates

96-Well
480 Plates
Catalog number HSS-9xxx HSP-9xxx HSL-9xxx HSR-99xx
Well height High-profile Low-profile Low-profile Low-profile
Skirt Semi-skirted Full-skirted Semi-skirted Semi-skirted
ANSI/SBS standard dimensions Yes Yes Yes Yes
Thermal Cycler Compatibility
Bio-Rad® C1000™, C1000 Touch™, S1000™
Bio-Rad® T100™      
Bio-Rad® DNA Engine®, DNA Engine Tetrad® and Tetrad 2, DNA Engine Dyad®, Dyad Disciple™    
Bio-Rad® iCycler®      
Applied Biosystems
0.2 ml tube cyclers
(2720, 9700, Veriti)
     
Applied Biosystems
0.1 ml tube cyclers
(9800 fast, Veriti fast)
     
Eppendorf Mastercycler series  
Real-Time PCR Detection System Compatibility
Bio-Rad® CFX Connect™, CFX96™, CFX96 Touch™    
Bio-Rad® CFX96 Touch Deep Well™    
Bio-Rad® iCycler iQ®, iQ™5, MyiQ™, MyiQ™2      
Bio-Rad® Chromo4™  
Bio-Rad® DNA Engine Opticon® and Opticon 2  
Applied Biosystems
standard
systems
(7300, 7500, 7900HT)
     
Applied Biosystems
fast systems
(7500 fast, ViiA 7 fast)
     
Roche LightCycler 480      
Eppendorf Mastercycler ep realplex
Stratagene Mx series      
Other Instrument Compatibility
Applied Biosystems DNA sequencers
(3700, 3730, etc.)
     
Idaho Technology LightScanner      

Recommended   Compatible



Hard-Shell 384-Well PCR Plates

 
384-Well
Standard Plates

384-Well
480 Plates
Catalog number HSP-3xxx HSR-48xx
Well height Standard 384-well height Standard 384-well height
Skirt Full-skirted Full-skirted
ANSI/SBS standard dimensions Yes Yes
Thermal Cycler Compatibility
Bio-Rad C1000, C1000 Touch, S1000
Bio-Rad DNA Engine, DNA Engine Tetrad and Tetrad 2, DNA Engine Dyad, Dyad Disciple
Applied Biosystems 384-well cyclers
Eppendorf Mastercycler series
Real-Time PCR Detection System Compatibility
Bio-Rad® CFX384™, CFX384 Touch™
Applied Biosystems 384-well systems
(7900HT, ViiA 7)
Roche LightCycler 480  
Other Instrument Compatibility
Idaho Technology LightScanner  

Recommended   Compatible

Hard-Shell® Technology

Two-Component Design for Superior Uniformity and Precise Handling

Hard-Shell PCR plates represent an innovative concept in PCR plate design. The skirt and deck are molded from a rigid thermostable polymer. This hard shell prevents the distortion and shrinkage that may occur when regular single-component polypropylene PCR plates are exposed to the high temperatures of thermal cycling or heat sealing. In a separate step, the thin-wall wells are molded of virgin polypropylene selected for low DNA binding. The footprint and well spacing of Hard-Shell skirted PCR plates meet ANSI/SBS microplate standards, and raised rims around each well allow tight sealing with a wide variety of sealing systems, including adhesive-, pressure-, and heat-sealing methods. Hard-Shell PCR plate attributes include:


  • Superior stability and flatness due to a patented* 2-component design, allowing precise positioning for automation
  • Extremely uniform wells that reduce well-to-well variability in optical assays, such as those performed in real-time PCR
  • Thin wall for fast PCR
  • Several plate styles that fit most thermal cyclers, real-time PCR detection systems, DNA sequencers, and plate readers
  • Black lettering for easy well identification
  • White-well option, which allows increased fluorescent signal strength
  • Color-coded skirts with clear wells
  • Low-cost, user-readable bar codes to enable convenient database tracking
  • Free of DNase, RNase, and human DNA



To view an audio slide show that describes the benefits of Hard-Shell plates, download one of the files below (Windows users, right click the link; Mac users, control click the link).


Windows (.wmv)   |   Macintosh (.mov)


* Hard-Shell plates are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977; 6,340,589; and 6,528,302.

Plate Dimensions
Specifications

Not drawn to scale.
All dimensions in mm, unless otherwise noted.

Microplate Dimensions Hard-Shell® 384-Well 480 PCR Plates (HSR-4xxx)
Length at base plane 127.76 mm
Width at base plane 85.48 mm
Height overall 10.40 mm
Well depth 9.35 mm
Well diameter at opening 3.10 mm
Well diameter at bottom of conical section 1.46 mm
Well volume 50 µl
Well spacing 4.50 mm
Well angle 17.5°
Well offset
  Left edge to well A1 12.13 mm
  Top edge to well A1 8.99 mm
  Left edge to P24 115.63 mm
  Top edge to P24 76.49 mm
Optical Guide

Well Color Effect on Signal Strength

Well color effect on signal strength

White well:

  • Increases fluorescent signal for maximizing detection sensitivity
  • Prevents fluorescent interference from adjacent wells or a contaminated cycler

Clear well:

  • Transparent wells for easy sample handling
  • Low signal variability for consistent results

Black well:

  • Lower signal strength for FP (fluorescence polarization) and other assays where reflected light must be minimized

Sealer Effect on Signal Strength

Sealer effect on signal strength

Optical flat cap strips:

  • Vapor-tight sealing for tube strips or 96-well plates
  • Suitable for PCR volumes >5 µl

Microseal® 'B' adhesive seals, optically clear:

  • A convenient sealing film for 96- or 384-well plates
  • Higher optical transmission than optical caps
  • Suitable for PCR volumes >10 µl

Clear Chill-out™ liquid wax:

  • No heated lid or lid pressure is required for PCR
  • Higher optical transmission than optical caps
  • Solidified below 10°C to prevent spilling and aerosol formation

Important Information
This product will be unavailable until late April 2014.
For replacement options in the U.S., call 1-800-4-BIORAD (1-800-424-6723). For replacement options outside the U.S., visit the Contact Us page.

 

Hard-Shell 384-well 480 PCR plates are optimized to work on the Roche LightCycler 480 real-time PCR system. They are specifically designed to withstand the stresses of thermal cycling and robotic handling. The patented* two-component design provides superior stability and flatness, allowing precise positioning for automation.

Key Features

  • Reaction volumes of 1–30 µl (50 µl maximum)
  • Extremely uniform wells reduce well-to-well variability in optical assays, such as those used in real-time PCR
  • Warp-free skirt and deck for improved robotic handling
  • Bar-coded on row A side; available in clear or white well
  • Black alphanumeric labeling for easy well identification
  • Footprint and well spacing match ANSI/SBS standard dimensions
  • Free of DNase, RNase, and human DNA

Compatible Instruments

  • Roche LightCycler 480 real-time PCR system
  • Bio-Rad C1000™, S1000™, and DNA Engine® family thermal cyclers
  • Bio-Rad CFX384™ real-time PCR detection system
  • Applied Biosystems thermal cyclers and real-time PCR systems
  • Eppendorf Mastercycler series

* Hard-Shell plates are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977; 6,340,589; and 6,528,302.

Hard-Shell® 384-Well 480 PCR Plates

HSR-4805
Pkg of 50, clear shell/white well PCR plate for Roche LightCycler 480 real-time PCR system, bar-coded, rigid 2-component design

List Price:   $276.00
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Hard-Shell® 384-Well 480 PCR Plates Kit

HSR-4805K
Kit for use with Roche LightCycler 480 real-time PCR system, includes 100 bar-coded clear shell/white well PCR plates (2 packs of #HSR-4805) and 100 Microseal® 'C' optical seals (#MSC-1001)

List Price:   $666.00
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Hard-Shell® 384-Well 480 PCR Plates

HSR-4801
Pkg of 50, clear shell/clear well PCR plate for Roche LightCycler 480 real-time PCR system, bar-coded, rigid 2-component design

List Price:   $276.00
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Hard-Shell® 384-Well 480 PCR Plates Kit

HSR-4801K
Kit for use with Roche LightCycler 480 real-time PCR system, includes 100 bar-coded clear shell/clear well PCR plates (2 packs of #HSR-4801) and 100 Microseal® 'C' optical seals (#MSC-1001)

List Price:   $666.00
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Number Description Options
10025848 Hard-Shell 480 PCR Plates Product Insert, Rev A Click to download
6090 Amplification Reagents and Plastics Brochure, Rev D Click to download [ Add to Cart (Free) ]
07237 Hard-Shell 384-Well PCR Plates Product Insert, Rev E Click to download
5496 Hard-Shell PCR Plates Brochure, Rev D Click to download