Got Protein? Kit

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Got Protein? Kit

Overview

Lab Preparation Checklist Lab Preparation Checklist
Laboratory Preparation Checklist
Kit contains sufficient materials for 80 student workstations. (2–4 students per workstation)
Quick Start Bradford Protein Assay Kit 4, includes 1x dye reagent (1 L), bovine γ-globulin standard set (2 sets of 7 standards, 0.125–2.0 mg/ml, 2 ml) 1
10x PBS, 100 ml 1
1.5 ml Standard Disposable Polystyrene Cuvettes, 100 1
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Required Accessories Not Included in Kit:
Adjustable micropipets
    2–20 µl 1–8
    100–1,000 µl 1
Pipet tips
    2–20 µl, TBR-35 1 box
    2–100 1,000 µl, TBR-40 1 box
Microtubes  1.5 ml 1 bag
15 ml Capped tubes 8
Distilled water 100 ml
Milk Samples (suggestions: low fat, fat free, soy, baby formula, etc.) 10 ml
Recommended (Optional) Accessories
SmartSpec Plus Spectrophotometer 1
Got Protein? Kit Flowchart Got Protein? Kit Flowchart
Got Protein? Kit Flowchart
Got Protein? Kit Flowchart

Key Kt Features Key Kit Features
Key Kit Features
  • Explore biophotonics
  • Study protein structure/function
  • Apply Beer's law
  • Measure protein concentrations
  • Learn spectrophotometry
  • Complete in one 45 minute lab session
Curriculum Fit Curriculum Fit
Curriculum Fit
Got Protein? Kit Curriculum Fit
Beer's Law and Bradford Method Beer's Law and Bradford Method
Beer's Law and the Bradford Method

Beer's law states that when a solute absorbs light of a particular wavelength, the absorbance is directly proportional to the concentration of substance in solution. The measurement most commonly used in protein assays is the absorbance of light.However, proteins do not absorb sufficient light to assay — by themselves.

The Bradford method is based on the color development formed when the dye Coomassie Blue G-250 binds to protein. The unique chemical properties of the dye allow it to interact with the side chains, or R-groups, of specific amino acids. There is a correlation between the amount of blue color and the amount of protein in the sample: the more protein, the more intense the blue color. The simplicity of the assay allows the results to be measured qualitatively by eye, or quantitatively with a spectrophotometer.

A qualitative view of a Bradford standard curve.
PowerPoint Presentation for Educational Use PowerPoint Presentation for Educational Use
PowerPoint Presentation for Workshops and for Teaching

Got Protein? Kit

This PowerPoint presentation is intended for educational use only. The presentation provides educators with background on the Got Protein? Kit and includes instructions for using the kit.

Got Protein? Kit Download PowerPoint presentation		 Got Protein? Kit


Based on the Bio-Rad Quick Start™ Bradford protein assay, the Got Protein™ Kit is designed to introduce students to proteomics and provides the tools for them to develop their own protein-based experiments. In this laboratory activity, students use absorbance values from a set of protein samples with known concentrations to create a standard curve on linear graph paper. Protein concentrations of their test samples, such as milk, egg, sports drink, or saliva, can then be extrapolated by hand or plotted using a graphing utility such as Microsoft Excel software.

Features and Benefits

  • Inquiry-based activity
  • Integrates biology, chemistry, and physics in relation to protein structure and function

With this kit, students are able to:

  • Measure protein concentrations
  • Apply Beer's law
  • Learn to use a spectrophotometer
  • Learn to use a micropipet

How It Works

The Bradford method is based on the color development formed when the dye Coomassie Blue G–250 binds to protein. There is a correlation between the amount of blue color and the amount of protein in the sample: the more protein, the more intense the blue color. The simplicity of the assay allows the results to be measured qualitatively by eye or quantitatively with a spectrophotometer.

More Information

  • One 45 min laboratory activity
  • Provides sufficient materials for eight student workstations, up to four students per workstation.
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)  
Quick Start™ Bradford Protein Assay Kit 4, includes 1 L of 1x dye reagent, bovine γ-globulin standard set (2 sets of 7 standards, 0.125–2.0 mg/ml, 2 ml) 1
10x PBS, 100 ml 1
1.5 ml standard disposable polystyrene cuvettes 100
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
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Got Protein? Kit

1662900EDU
Introductory proteomics classroom study kit, includes Quick Start Bradford protein assay kit 4 (#500-0204EDU), 10x PBS, curriculum, and more, for 320 students; education use only

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5516 Got Protein? Kit, Biotechnology Explorer, Quick Guide, Rev A Click to download
10006836 Got Protein? Kit, Biotechnology Explorer, Instruction Manual, Rev A Please login to download this document