GMO Investigator Real-Time PCR Starter Kit

Print
GMO Investigator Real-Time PCR Starter Kit

Overview

Lab Preparation Checklist
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation), plus additional real-time reagents for further studies
GMO Investigator kit 1
iQ SYBR® Green Supermix, 5 ml 1
Agarose powder, 25 g 1
Electrophoresis buffer, 50x TAE, 100 ml 1
Fast Blast DNA stain, 500x, 100 ml 1
Sterile water, 500 ml 1
PCR tube strips 120
Optical flat caps 120
Curriculum, including Teacher's Guide and Student Manual 1
Required Accessories Not Included in Kits:
Horizontal gel electrophoresis chambers 4–8
Adjustable micropipets
   2–20 μl 1–8
   20–200 μl 1
   100–1,000 μl 1
Pipet tips, aerosol barrier  
   2–1,000 µl, Xcluda B, D, and E  
Power supplies 2–4
Real-Time thermal cycler 1
Microcentrifuges 1–4
Additional Required Accessories Not Included in GMO Investigator Real-Time PCR Starter Kit:
Obtain food samples locally  
Water bath 1
Mortars and pestles 1–8
Balance with range 0.5–2 g 1
Weigh boats or paper 16
Recommended (Optional) Accessories:
Rocking platform  
Microwave oven  
Real-Time PCR Flowchart
Real-Time PCR Flowchart
Key Kit Features
  • Quantitate DNA
  • Discover key differences between conventional and real-time PCR analysis
  • Analyze and evaluate real-time PCR results
  • Perform melt-curve analysis
  • Determine the accuracy and reliability of pipetting techniques
  • Learn the molecular basis of DNA amplification reactions using real-time PCR detection systems
Curriculum Fit
Curriculum Fit
Conventional PCR Animation

Content on this page requires a newer version of Adobe Flash Player.

Get Adobe Flash player

Sample Real-Time PCR Results
Sample Real-Time PCR Results

Sample Real-Time PCR Results.

Polymerase chain reaction (PCR) is now such a fundamental technique in the biotechnology laboratory that L. A. Pray wrote in 2004: "PCR is to biology what petroleum is to transportation." PCR is a basis for multiple ways — ranging from DNA fingerprinting and sequencing to mutagenesis — to analyze and detect nucleic acids. Real-time PCR is an extremely valuable analytical tool that not only reveals what DNA is present but how much. Real-time PCR is becoming the most widely used PCR application for genomic and gene expression analysis in research laboratories; it also is rapidly establishing itself as a technique in the clinical diagnostic lab. The need for faster, more accurate, and more economical systems with a high throughput has fueled the popularity of real-time PCR.

Using genomic DNA as the template for amplification, real-time PCR can be used in infectious disease diagnostics to rapidly determine levels of specific pathogens in various tissues. The molecular diagnostic laboratory also relies heavily on real-time PCR for detecting aneuploidies and diagnosising other genetic diseases. In microbiology laboratories, real-time PCR can be used to detect and quantitate various microbial contaminants in environmental samples.

The GMO Investigator real-time PCR starter kit is designed for teaching students the principles of PCR and its use in testing foods for the presence of genetic modifications. This kit can be used to quantitate the amount of DNA in a plant and to compare it with the level of genetically modified DNA that is recovered from each food sample. It is even possible to determine what fraction of a food product is made with genetically modified ingredients, in the same manner as standard testing laboratories do.

Reference

Pray LA (2004). Consider the Cycler, The Scientist 18, 34–37.

 

 

 

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation), plus additional real-time reagents for further studies  
GMO Investigator kit 1
iQ™ SYBR® Green Supermix, 5 ml 1
Agarose powder, 25 g 1
Electrophoresis buffer, 50x TAE, 100 ml 1
Fast Blast™ DNA stain, 500x, 100 ml 1
Sterile water, 500 ml 1
PCR tube strips 120
Optical flat caps 120
Curriculum, including Teacher's Guide and Student Manual 1
GMO Real-Time PCR Starter Kit

166-2560EDU
Introductory real-time PCR classroom study kit, contains GMO Investigator kit (#166-2500) and additional real-time reagents; education use only

List Price:   $686.25
EDU Price:   $549.00   What is this?
Quantity:   Add to cart
Add to Hot List   Add to Quote
Number Description Options
10002155 Instruction Manual, GMO Investigator Kit, Biotechnology Explorer, Rev C Please login to download this document [ Add to Cart (Free) ]
M1662505 GMO Investigator Kit: A Quantitative Real-Time PCR Extension, Biotechnology Explorer, Application Note, Rev A Please login to download this document
5396 Fast Gel Protocol. Run Agarose DNA Gels in Under 20 Minutes, Biotechnology Explorer, Rev A Click to download