Gel Doc XR+ Gel Documentation System

Gel Doc XR+ Gel Documentation System

The Gel Doc XR+ System is a fast, easy-to-use, completely automated gel imaging and documentation system controlled by Image Lab Image Capture and Analysis Software.

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Overview

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The Gel Doc XR+ System is based on CCD high-resolution, high-sensitivity detection technology and modular options to accommodate a wide range of samples and support multiple detection methods including fluorescence and colorimetric detection. The Gel Doc XR+ System is controlled by Image Lab Software to optimize imager performance for fast, integrated, and automated image capture and analysis of various samples.

The system accommodates a wide array of samples, from large handcast polyacrylamide gels to small ReadyAgarose Gels and various blots. The system is an ideal accompaniment to PCR, purification, and electrophoresis systems, enabling image analysis and documentation of restriction digests, amplified nucleic acids, genetic fingerprinting, RFLPs, and protein purification and characterization.

Features and Benefits of the Gel Doc XR+ System

  • Gel and blot imaging and analysis are quick and accurate
  • Automated, hands-off routines; no training is required
  • Save and recall all the steps in the workflow for repeatable and reproducible results
  • Optimize the system at setup for image data that is always accurate, reproducible, and free of imaging artifacts
  • Wide range of applications with special accessories to preserve sample integrity for downstream research while ensuring user safety
  • Comprehensive, automated quantitative analysis of protein and DNA samples in seconds
  • Customize and organize data in reports
  • Obtain publication-quality results quickly

The Gel Doc XR+ System optimizes reproducibility and reliability of experimental data, enabling quantitative comparisons between different experiments. Using proprietary algorithms, this imaging system is calibrated at setup to ensure:

  • Images are in focus at all times, regardless of zoom level or sample position
  • Appropriate flat fielding correction is automatically and consistently applied to image data for every application
  • Imaging artifacts are automatically corrected

The Gel Doc XR+ System enables quick and easy visualization, documentation, and analysis of nucleic acid and protein gels, blots, and macroarrays with a few clicks of the mouse. The system supports fluorescence and colorimetric detection methods. The Gel Doc XR+ System consists of a darkroom hood, CCD camera and software-controlled motorized lens, UV and white light illuminators, filter slider with standard filter, and UV-protection shield. The system enables you to:

  • Increase cloning efficiency and protein production by protecting DNA electrophoresis samples from UV exposure using the XcitaBlue conversion screen and blue light excitable stains such as GelGreen, SYBR Safe, and SYBR Green I
  • Maintain standard operating procedures or criteria for sample performance as there is no loss in sensitivity compared to UV and ethidium bromide staining
  • For imaging SYBR Safe DNA applications, you may use the UV transilluminator or the optional XcitaBlue kit. The XcitaBlue (UV to blue conversion screen) protects samples from UV damage and also allows you to externally visualize DNA samples without a UV shield

The Gel Doc XR+ System can be upgraded to the ChemiDoc XRS+ System.

Specifications

Applications
 
Chemiluminescence
No
Fluorescence*
Yes
Colorimetry/densitometry
Yes
Gel documentation
Yes
Hardware Specifications
 
Maximum sample size
28 x 36 cm
Maximum image area
19.4 x 26 cm
Excitation source
Epi-white light and trans-UV (302 nm) are standard (optional 365 nm lamp available); optional trans-white conversion screen and XcitaBlue™ UV/blue conversion screen available
Illumination control
3 modes (trans-UV, trans white, epi-white)
Detector
CCD
Image resolution
4 megapixels
Pixel size (H x V) 
4.65 x 4.65 µm
Filter holder
3 positions (2 for filters, 1 without filter)
Emission filters
1 included (standard), 3 optional
Dynamic range
>3.0 orders of magnitude
Pixel density (gray levels)
4,096
Dynamic flat fielding
Application specific, for all applications
Instrument size (L x W x H)
36 x 60 x 96 cm
Instrument weight
32 kg
Operating Ranges
 
Operating voltage
110/115/230 V AC nominal
Operating temperature
10–28°C (21°C recommended)
Operating humidity
<70% noncondensing
Automation Capabilities
 
Workflow automated selection
Application driven; user selected or recalled by a protocol
Workflow automated execution
Controlled by a protocol via application specific setup for image area, illumination source, filter, analysis, and reporting
Workflow reproducibility
100% repeatability via recallable protocols; from image capture to quantitative analysis and reports
Autofocus (patent pending)
Precalibrated focus for any zoom setting or sample height
Image flat fielding**
Dynamic; precalibrated and optimized per application
Autoexposure
2 user-defined modes (intense or faint bands)
*Use the optional XcitaBlue kit if performing SYBR® Safe DNA applications, because the UV to blue conversion screen allows you to visualize DNA samples while protecting against UV damage.

**U. S. patent 5,951,838.
 

Accessories

Image
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1708199
Upgrade package for Gel Doc XR+ system includes Image Lab software, focus calibration target, flat fielding disk, installation guide, and user manual
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1708026
Pkg of 1, calibration target for Gel Doc™ XR+, ChemiDoc™ XRS+, and ChemiDoc MP imaging systems with Image Lab™ software
1708279
Pkg of 1, UV safety shield to protect users from UV light exposure during band excision; for use with all ChemiDoc and GelDoc Imaging Systems from the universal hood series
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1708289
Pkg of 1, conversion screen for viewing Coomassie, silver stain, and other colorimetric gel stains; for use with non touch–screen ChemiDoc™ MP, ChemiDoc™ XRS+, and Gel Doc™ XR+ Systems
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1708182
Pkg of 1, conversion screen for viewing SYBR® Green, SYBR® Safe, GFP, and Flamingo™ Fluorescent Gel Stains, includes viewing goggles; for use with ChemiDoc™ XRS+, non touch–screen ChemiDoc MP, and Gel Doc™ XR+ Systems
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1708184
Pkg of 3 gel templates, for use with ChemiDoc™ XRS+, ChemiDoc MP, and Gel Doc™ XR+ Systems, includes template holder
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(Discontinued) Filter
1708077
Pkg of 1, filter, 480BP70 62 mm, for Hoechst/coumarin, for use with Gel Doc XR and ChemiDoc XRS systems

Documents

Number Description Options
5838
Gel Doc™ XR+ Imaging System Brochure, Rev G
[ Add to Cart (Free) ]
2577
Instruction Manual, Molecular Imager Gel Doc XR and ChemiDoc XRS Systems Installation Guide, Rev B
10014084
Instruction Manual, GelDoc XR and ChemiDoc XRS, Rev A
10000076955
Gel Doc™ XR+ and ChemiDoc™ XRS+ Imaging Systems with Image Lab™ Software Instrument Guide
10016116
Gel Alignment Template Kit
4000177
Instruction Manual, IPD Multi-Color Imaging Target, Rev A
10040758
Instruction Manual, Image Lab™ Software: Imaging Stain-Free Gels and Blots, Rev B
6434
Western Blot Normalization Using Image Lab™ Software, Quick Start Guide
[ Add to Cart (Free) ]
6427
Bio-Rad Stain-Free Western Blotting
[ Add to Cart (Free) ]
2589
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