MicroRotoforâ„¢ Cell

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Overview

Features and Benefits
Feature Benefit
Design
Small sample volume • Accommodates researchers with limited sample volumes
• Allows optimization of separation protocols
• Pairs with other sample preparation solutions (for example, MicroRotofor lysis kits, Aurum serum protein mini kit, ReadyPrep 2-D cleanup kit)
• Allows refractionation of fractions from Rotofor and mini Rotofor runs
Easy assembly • User friendly
• Saves time
10 fractions
(200–250 µl/fraction)
• Allows thorough screening of protein samples
• Up to 10-fold concentration of proteins of interest
• Fractions may be pooled if fewer fractions are desired
Integrated Peltier
cooling (2 settings,
~20°C or ~10°C)
• Maintains stable temperature during focusing for greater reproducibility
• Prevents proteolysis and carbamylation of proteins
• Capacity for native separations (to preserve protein structure and function)
Compact profile • Fits on any laboratory bench
Technology
Liquid separation medium • Separates proteins that are insoluble in other media (for example, hydrophobic, membrane, and large proteins)
• Pairs easily with other separation strategies
• Capacity for native separations
Membrane core • Stabilizes protein fractions for harvesting without impeding flow throughout chamber
Continuous pH gradient/customizable pH setup • Allows screening of proteins by pI
• Enables resolution of isoforms
• Flexible — allows determination of pH range for fractionation
• Can be matched to micro- and narrow-range IPG strips
Vacuum-assisted harvesting • Quick, easy
• Prevents cross-contamination of fractions
Disposable chambers • Convenient
• Prevent cross-contamination of samples
Rocking • Allows mixing
• Mitigates protein precipitation and convective influences
Rotofor and MicroRotofor Tutorials
PROTEAN i12 IEF System - Gel-Side Up Cup Loading Assembly
2-D Electrophoresis Tutorials
PDQuest Software Tutorials

The MicroRotofor cell uses liquid-phase IEF to fractionate small volumes (2.5 ml) of proteins in free solution by their pI. The MicroRotofor cell can be used to reduce the complexity of samples prior to any protein analysis technique (including mass spectrometry and 2-D electrophoresis). In 2-D electrophoresis applications, protein fractionation by pI improves downstream sample loading and separation on narrow- and micro-range IPG strips. Whether used alone or as part of a broader separation or purification scheme, the MicroRotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures.

The MicroRotofor cell is easy to set up and offers:

  • Unique liquid-phase fractionation by pI, eliminating proteins outside a pI range of interest
  • Up to tenfold concentrations of proteins of interest
  • Customizable pH gradients — wide or narrow linear pH gradients can be generated using Bio-Lyte® ampholytes
  • Rapid recovery of proteins focused into 10 liquid fractions
  • Integrated temperature regulation to improve reproducibility and to preserve protein structure and function for native separations
  • Preassembled disposable focusing chambers

Accessory equipment required to run the MicroRotofor cell includes a 1,000 V power supply such as the PowerPac™ HV power supply (which is capable of running at 1 W constant power) and house vacuum. Bio-Lyte ampholytes are required to form the internal pH gradient.

Related Products

Different configurations of the Rotofor system are available to accommodate different sample loads. The Rotofor®, Mini Rotofor, and MicroRotofor cells all share the following features:

  • Interchangeable cylindrical focusing chambers, which enable refractionation of selected fractions for even greater purification
  • Maintenance of biological activity
  • Customizable pH gradients — wide or narrow linear pH gradients can be generated using Bio-Lyte ampholytes
  • Focusing and enrichment of a protein of interest into 1 or 2 fractions in a single step

 

  Rotofor Cell Mini Rotofor Cell MicroRotofor Cell
Number of fractions 20 20 10
Sample volume 35–60 ml 18 ml 2.5 ml
Fraction volume 1.75–3 ml 0.7 ml 200–250 µl
Sample load Milligram to gram Microgram to milligram Up to to 1 mg


MicroRotofor cell lysis kits provide convenient cell lysis and protein extraction protocols to generate total protein samples that are ready for fractionation with a MicroRotofor IEF cell or other downstream applications such as SDS-PAGE and 2-D gel electrophoresis. Kits are available for mammalian, bacterial, plant, and yeast cells.

(Discontinued) MicroRotofor Cell Kit

170-2800
100/120 V, preparative IEF device, with accessories (high voltage power supply required, #164-5056 sold separately)

This product is no longer available. Please contact technical support for more information on replacement product.
MicroRotofor Cell Kit

170-2801
220/240 V, preparative IEF device, with accessories (high voltage power supply required, #164-5056 sold separately)

List Price:   $4,048.00
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MicroRotofor System

170-2802
100/120 V, preparative IEF device with accessories (#170-2800) with high voltage power supply (#164-5056)

List Price:   $6,273.00
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MicroRotofor System

170-2803
220/240 V, preparative IEF device with accessories (#170-2800) with high voltage power supply (#164-5052)

List Price:   $6,385.00
Quantity:   Add to Quote
Add to Hot List  
Number Description Options
5451 First-Dimension Separation With the MicroRotofor Cell Prior to SDS-PAGE and LC-MS/MS Analysis, Rev A Click to download
5349 Fractionation by Liquid-Based Isoelectric Focusing With the MicroRotofor Cell: Improving the Resolution of Low-Abundance Proteins on 2-D Gels (Poster), Rev A Click to download
5344 Fractionation by Liquid-Phase Isoelectric Focusing in the MicroRotofor Cell: Improved Detection of Low-Abundance Proteins, Rev A Click to download
10004399 Instruction Manual, MicroRotofor Cell Starter Kit Manual, Rev A Click to download
10008759 MicroRotofor Cell Tutorial DVD [ Add to Cart (Free) ]
5294 MicroRotofor Cell Product Information Sheet Click to download