Foresight™ Prepacked Chromatography Columns

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Overview

Foresight™ Filter Plates and Columns Workflow

Foresight chromatography filter plates and columns are prepacked with a range of Bio-Rad’s process chromatography media, offering process scientists convenience and reliability for their high-throughput (HT) experimentation needs. Their robust design allows process scientists to use the prepacked formats through the entire purification development cycle, from HT media screening to small-scale methods development and scale-up optimization.
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Purification scale-up workflow shows the use of HT screening and design of experiments (DOE) to define an operational window for biomanufacturing.

 

Using High-Throughput (HT) Methods to Accelerate DOE

High-throughput experimentation (HTE) was used in this case study in concert with design of experiments (DOE) methodology to study the mass recovery of a basic therapeutic protein (pI 9.2–9.5) using a hydrophobic cation exchange mixed-mode resin.



Foresight Prepacked Chromatography Columns have a robust design that provides convenience and reliability for process scientists. Foresight Columns may be used for media screening experiments and/or small-scale method development. Through design of experiments (DOE) utilizing Foresight Columns, an operational window can be defined to aid in developing a quality by design (QbD) process. The design of the column ensures leak-free operation at pressures up to 30 bar.* The columns allow faster, more convenient experimental setups.

The design of Foresight Prepacked Chromatography Columns offers:

  • Convenience — prepacked column is ready for equilibration in the buffer of choice. Multiple bed heights are available with the same column diameter that allow straightforward comparative evaluation
  • Reliability — each column is individually packed under optimum compression, ensuring consistent experimental results

The Foresight Columns Instruction Manual describes how to use Foresight Columns prepacked with Bio-Rad's Chromatography Resins.

Other formats available:

Learn more about Foresight Prepacked Column and Plate formats and applications.

Chromatography Resins Available in Foresight Prepacked Columns and Plates
Chromatography Resin Mode
UNOsphere™ Q Strong anion
UNOsphere S Strong cation
Nuvia™ Q Strong anion
Nuvia S Strong cation
Nuvia™ cPrime™ Mixed mode
Hydrophobic: cationic
Nuvia IMAC Metal affinity
CHT™ Type I — 40 μm particle size Mixed mode
Metal affinity: cationic
CHT Type II — 40 µm particle size Mixed mode
Metal affinity: cationic
MPC™ Mixed mode
Metal affinity: cationic
  

* Please refer to each resins' manual for pressure limitations. Do not exceed the resins' pressure limit during operation of the prepacked column.

 

1- and 5-ml Columns  
Connectors 10–32 fittings
Column material Polyethylene (HDPE)
Frit material Polypropylene/polyethylene
Autoclavability Not autoclavable
Shipping Conditions  
Ion exchangers  
  Nuvia™ S, Q and UNOsphere™ S, Q Media 20% ethanol + 150 mM NaCl
CHT™ and MPC™ Media 20% EtOH + 10mM sodium phosphate, pH 6.8
Nuvia™ cPrime™, Nuvia IMAC, UNOsphere SUPrA media 20% ethanol
1-ml Column  
Inner diameter, mm 8
Length, mm 20
Maximum pressure, bar (psi) Follow guidelines for each resin
Recommended flow rate, ml/min (cm/hr) 0.5–1 (59.5–119)
Maximum flow rate, ml/min (cm/hr) 8 (952)
5-ml Column  
Inner diameter, mm 8
Length, mm 100
Maximum pressure, bar (psi) Follow guidelines for each resin
Recommended flow rate, ml/min (cm/hr) 2.5–5 (297.5–595)
Maximum flow rate, ml/min (cm/hr) 8 (952)
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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Foresight™ Nuvia™ Ni-Charged IMAC, 1 ml column

12004038
Pkg of 1, 1 ml column prepacked with Nuvia IMAC Affinity Chromatography Resin

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$157.02
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Foresight™ Nuvia™ Ni-Charged IMAC, 5 ml column

12004037
Pkg of 1, 5 ml column prepacked with Nuvia IMAC Affinity Chromatography Resin

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$296.26
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Foresight™ MPC™ Type I Column, 40 µm, 1 ml
Foresight™ MPC™ Type I Column, 40 µm, 1 ml

7324737
Pkg of 1, 1 ml, column prepacked with MPC Type I Mixed-Mode Chromatography Resin, 40 µm particle size

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$149.00
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Foresight™ MPC™ Type I Column, 40 µm, 5 ml
Foresight™ MPC™ Type I Column, 40 µm, 5 ml

7324757
Pkg of 1, 5 ml, column prepacked with MPC Type I Mixed-Mode Chromatography Resin, 40 µm particle size

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$291.00
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Foresight™ Nuvia™ HR-S Column, 1 ml
Foresight™ Nuvia™ HR-S Column, 1 ml

7324723
Pkg of 1, 1 ml, column prepacked with Nuvia HR-S Strong Cation Exchange Chromatography Resin

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$127.00
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Foresight™ Nuvia™ HR-S Column, 5 ml
Foresight™ Nuvia™ HR-S Column, 5 ml

7324743
Pkg of 1, 5 ml, column prepacked with Nuvia HR-S Strong Cation Exchange Chromatography Resin

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$232.00
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Foresight™ UNOsphere SUPrA™ Column, 1 ml
Foresight™ UNOsphere SUPrA™ Column, 1 ml

7324729
Pkg of 1, 1 ml, column prepacked with UNOsphere SUPrA Affinity Chromatography Resin

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$209.00
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Foresight™ UNOsphere SUPrA™ Column, 5 ml
Foresight™ UNOsphere SUPrA™ Column, 5 ml

7324749
Pkg of 1, 5 ml, column prepacked with UNOsphere SUPrA Affinity Chromatography Resin

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$551.00
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Foresight™ UNOsphere™ Q Column

7324732
Pkg of 1, 1 ml, column prepacked with UNOsphere Q Strong Anion Exchange Chromatography Media

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$104.00
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Foresight™ UNOsphere™ Q Column

7324752
Pkg of 1, 5 ml, column prepacked with UNOsphere Q Strong Anion Exchange Chromatography Media

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$192.00
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Foresight™ UNOsphere™ S Column

7324730
Pkg of 1, 1 ml, column prepacked with UNOsphere S Strong Cation Exchange Chromatography Media

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$104.00
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Foresight™ UNOsphere™ S Column

7324750
Pkg of 1, 5 ml, column prepacked with UNOsphere S Strong Cation Exchange Chromatography Media

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$192.00
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Foresight™ Nuvia™ Q Column

7324721
Pkg of 1, 1 ml, column prepacked with Nuvia Q Strong Anion Exchange Chromatography Media

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$116.00
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Foresight™ Nuvia™ Q Column

7324741
Pkg of 1, 5 ml, column prepacked with Nuvia Q Strong Anion Exchange Chromatography Media

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$213.00
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Foresight™ Nuvia™ S Column

7324720
Pkg of 1, 1 ml, column prepacked with Nuvia S Strong Cation Exchange Chromatography Media

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$116.00
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Foresight™ Nuvia™ S Column

7324740
Pkg of 1, 5 ml, column prepacked with Nuvia S Strong Cation Exchange Chromatography Media

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$213.00
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Foresight™ Nuvia™ cPrime™ Column

7324722
Pkg of 1, 1 ml, column prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media

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$142.00
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Foresight™ Nuvia™ cPrime™ Column

7324742
Pkg of 1, 5 ml, column prepacked with Nuvia cPrime Hydrophobic Cation Exchange Chromatography Media

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$278.00
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Foresight™ CHT™ Type I Column

7324735
Pkg of 1, 1 ml, column prepacked with CHT Ceramic Hydroxyapatite Type I Multimodal Chromatography Media, 40 μm particle size

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$149.00
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Foresight™ CHT™ Type I Column

7324755
Pkg of 1, 5 ml, column prepacked with CHT Ceramic Hydroxyapatite Type I Multimodal Chromatography Media, 40 μm particle size

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$291.00
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Foresight™ CHT™ Type II Column

7324736
Pkg of 1, 1 ml, column prepacked with CHT Ceramic Hydroxyapatite Type II Multimodal Chromatography Media, 40 μm particle size

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$149.00
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Foresight™ CHT™ Type II Column

7324756
Pkg of 1, 5 ml, column prepacked with CHT Ceramic Hydroxyapatite Type II Multimodal Chromatography Media, 40 μm particle size

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$291.00
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Number Description Options
10024831 Instruction Manual, Foresight™ Chromatography Media Columns 1 and 5 ml, Rev A Click to download
6297 Foresight™ Prepacked Plates and Columns Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
10027565 Instruction Manual, Foresight™ RoboColumn Units 200 and 600 µl, Rev A Click to download
10024413 Instruction Manual, Foresight™ Chromatography Media Filter Plates, Rev A Click to download
D116698 Instruction Manual, Nuvia™ IMAC Resin Click to download
10023853 Instruction Manual, Nuvia™ cPrime™ Hydrophobic Cation Exchange Resin, Rev B Click to download
10033316 Instruction Manual, Nuvia HR-S Strong Cation Exchange Media, Rev A Click to download
10018215 Instruction Manual, Nuvia S and Q High-Capacity Ion Exchange Media, Rev C Click to download [ Add to Cart (Free) ]
4110109 Instruction Manual, UNOsphere Q and S Ion Exchange Media, Rev C Click to download
10014430 Instruction Manual, UNOsphere SUPrA™ Affinity Chromatography Media, Rev C Click to download
6086 Ceramic Hydroxyapatite Application Guide for Process Development and Scale-Up Click to download [ Add to Cart (Free) ]
6498 Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C Click to download [ Add to Cart (Free) ]
6982 Mixed-Mode Resins/Media Selection Card Click to download [ Add to Cart (Free) ]
6983 Native and Recombinant Protein Purification Resins/Media Selection Card Click to download
6786 Monoclonal Antibody Purification Resin Selection Card, Ver A Click to download
6789 IgG Polyclonal Antibody Purification Resin Selection Card, Ver A Click to download
6791 Virus Purification Resin Selection Card, Ver A Click to download
6792 Aggregate Purification Resin Selection Card, Ver A Click to download
6530 Protein Purification Workflow Development Using Bio-Rad’s NGC™ Chromatography System and Stain-Free Gel Technology, Rev D Click to download [ Add to Cart (Free) ]
6719 Development of an Efficient Manufacturing Process for Adenovirus, Ver A Click to download