Foresight™ Chromatography Columns, Prepacked

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Overview

Foresight™ Prepacked Chromatography Plates and Columns Selection Guide
Foresight Plates

Foresight plates are 96-well filter plates prepacked with 20 µl of chromatography media in each well.

Foresight RoboColumn Units

Foresight RoboColumn units are offered as single rows of eight prepacked microcolumns. Foresight RoboColumn units are ideal for high-throughput (HT) process development.

Foresight Columns

Foresight columns are ideal for confirming the operational window obtained through design of experiments (DOE) in trials with Foresight plates and/or Foresight RoboColumn units.

Applications

Foresight plates can be used for HT evaluation of various binding, elution, or wash conditions for different chromatography media. They can be processed using manual or automated robotic systems, and sample collection may be achieved through centrifugation or vacuum manifold techniques.

Foresight RoboColumn units are fully compatible with robotic liquid handling systems, allowing process developers to perform automated HT parallel media screening and/or small-scale method development. Multiple rows up to 12 can be combined to form a 96-column array, allowing users to evaluate various chromatography media and conditions in parallel.

Foresight columns may be used for media screening experiments and/or small-scale method development. Foresight columns have standard HPLC connections, which make them compatible with the NGC™ chromatography system and other liquid chromatography systems.

Physical Dimensions

Standard 96-well plate format
128 × 86 × 33 mm

1 × 8 row
128 × 86 × 14 mm

8 × 20 mm (1-ml column)
8 × 100 mm (5-ml column)

Working Volume

<900 µl

200–600 µl

1–5 ml

Recommended Flow Rate, cm/hr

N/A

16–1,000

59.5-119 (1-ml column)
287.5-595 (5-ml column)

Maximum Pressure, bar (psi)

N/A

8 (116)

30 (435)

Media Types Available

UNOsphere™ Q, UNOsphere S, UNOsphere Rapid S; Nuvia™ Q, Nuvia S, Nuvia™ cPrime™; CHT™ Type I, CHT Type II

Ordering Information
Foresight™ Filter Plates and Columns Workflow

Foresight chromatography filter plates and columns are prepacked with a range of Bio-Rad’s process chromatography media, offering process scientists convenience and reliability for their high-throughput (HT) experimentation needs. Their robust design allows process scientists to use the prepacked formats through the entire purification development cycle, from HT media screening to small-scale methods development and scale-up optimization.
Learn more


Purification scale-up workflow shows the use of HT screening and design of experiments (DOE) to define an operational window for biomanufacturing.

 

Using High-Throughput (HT) Methods to Accelerate DOE

High-throughput experimentation (HTE) was used in this case study in concert with design of experiments (DOE) methodology to study the mass recovery of a basic therapeutic protein (pI 9.2–9.5) using a hydrophobic cation exchange mixed-mode resin.

Foresight chromatography columns have a robust design that provides convenience and reliability for process scientists. Foresight columns may be used for media screening experiments and/or small-scale method development. Through design of experiments (DOE) utilizing Foresight columns, an operational window can be defined to aid in developing a quality by design (QbD) process. The design of the column ensures leak-free operation at pressures up to 30 bar. The columns allow faster, more convenient experimental setups.

The design of Foresight columns offers:

  • Convenience — prepacked column is ready for equilibration in the buffer of choice. Bed heights are available in ratios that allow straightforward comparative evaluation
  • Reliability — each column is individually packed under optimum compression, ensuring consistent experimental results

The instruction manual describes how to use Foresight columns prepacked with Bio-Rad chromatography media.

Other formats available:

Learn more about Foresight prepacked formats and applications.


Chromatography Media Available in Foresight Formats
Chromatography Media Mode
UNOsphere™ Q Strong anion
UNOsphere S Strong cation
UNOsphere Rapid S Strong cation
Nuvia™ Q Strong anion
Nuvia S Strong cation
Nuvia™ cPrime™ Mixed mode
Hydrophobic: cationic
CHT™ Type I — 40 μm particle size Mixed mode
Metal affinity: cationic
CHT Type II — 40 µm particle size Mixed mode
Metal affinity: cationic
  

 

1- and 5-ml Columns  
Connectors 10–32 fittings
Column material Polyethylene (HDPE)
Frit material Polypropylene/polyethylene
Storage 20% ethanol or 0.1 N NaOH
Autoclavability Not autoclavable
Shipping Conditions  
Ion exchangers 20% ethanol + 0.15 M NaCl
CHT™ media Dry
Nuvia™ cPrime™ media 20% ethanol
1-ml Column  
Inner diameter, mm 8
Length, mm 20
Maximum pressure, bar (psi) 20 (290)
Recommended flow rate, ml/min (cm/hr) 0.5–1 (59.5–119)
Maximum flow rate, ml/min (cm/hr) 8 (952)
5-ml Column  
Inner diameter, mm 8
Length, mm 100
Maximum pressure, bar (psi) 30 (435)
Recommended flow rate, ml/min (cm/hr) 2.5–5 (297.5–595)
Maximum flow rate, ml/min (cm/hr) 8 (952)
Foresight™ UNOsphere™ Q Column

732-4732
Pkg of 1, 1 ml, column prepacked with UNOsphere Q strong anion exchange chromatography media

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Foresight™ UNOsphere™ Q Column

732-4752
Pkg of 1, 5 ml, column prepacked with UNOsphere Q strong anion exchange chromatography media

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Foresight™ UNOsphere™ S Column

732-4730
Pkg of 1, 1 ml, column prepacked with UNOsphere S strong cation exchange chromatography media

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Foresight™ UNOsphere™ S Column

732-4750
Pkg of 1, 5 ml, column prepacked with UNOsphere S strong cation exchange chromatography media

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Foresight™ UNOsphere™ Rapid S Column

732-4731
Pkg of 1, 1 ml, column prepacked with UNOsphere Rapid S strong cation exchange chromatography media

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Foresight™ UNOsphere™ Rapid S Column

732-4751
Pkg of 1, 5 ml, column prepacked with UNOsphere Rapid S strong cation exchange chromatography media

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Foresight™ Nuvia™ Q Column

732-4721
Pkg of 1, 1 ml, column prepacked with Nuvia Q strong anion exchange chromatography media

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Foresight™ Nuvia™ Q Column

732-4741
Pkg of 1, 5 ml, column prepacked with Nuvia Q strong anion exchange chromatography media

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Foresight™ Nuvia™ S Column

732-4720
Pkg of 1, 1 ml, column prepacked with Nuvia S strong cation exchange chromatography media

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Foresight™ Nuvia™ S Column

732-4740
Pkg of 1, 5 ml, column prepacked with Nuvia S strong cation exchange chromatography media

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Foresight™ Nuvia™ cPrime™ Column

732-4722
Pkg of 1, 1 ml, column prepacked with Nuvia cPrime hydrophobic cation exchange chromatography media

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Foresight™ Nuvia™ cPrime™ Column

732-4742
Pkg of 1, 5 ml, column prepacked with Nuvia cPrime hydrophobic cation exchange chromatography media

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Foresight™ CHT™ Type I Column

732-4735
Pkg of 1, 1 ml, column prepacked with CHT ceramic hydroxyapatite Type I multimodal chromatography media, 40 μm particle size

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Foresight™ CHT™ Type I Column

732-4755
Pkg of 1, 5 ml, column prepacked with CHT ceramic hydroxyapatite Type I multimodal chromatography media, 40 μm particle size

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Foresight™ CHT™ Type II Column

732-4736
Pkg of 1, 1 ml, column prepacked with CHT ceramic hydroxyapatite Type II multimodal chromatography media, 40 μm particle size

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Foresight™ CHT™ Type II Column

732-4756
Pkg of 1, 5 ml, column prepacked with CHT ceramic hydroxyapatite Type II multimodal chromatography media, 40 μm particle size

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Number Description Options
6498 Lab-Scale Protein Purification Column and Cartridge Selection Guide, Rev A Click to download [ Add to Cart (Free) ]
6297 Foresight Prepacked Plates and Columns Product Information Sheet, Rev A Click to download
10024831 Instruction Manual, Foresight™ Chromatography Media Columns 1 and 5 ml, Rev A Click to download
10027565 Instruction Manual, Foresight™ RoboColumn Units 200 and 600 µl, Rev A Click to download
10024413 Instruction Manual, Foresight™ Chromatography Media Filter Plates, Rev A Click to download
6530 Protein Purification Workflow Development Using Bio-Rad’s NGC Chromatography System, Rev B Click to download