Fluorescent Assay Kits

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Overview

Filter Selection Guide
Application Excitation Filter Catalog # Emission Filter Catalog #
DNA quantitation (Hoechst 33258) EX 360/40 (340–380 nm) 170-2420 EM 460/10 (455–465 nm) 170-2421
β-Galactosidase (MUG) EX 360/40 (340–380 nm) 170-2420 EM 460/10 (455–465 nm) 170-2421
β-Glucuronidase (MUGluc) EX 360/40 (340–380 nm) 170-2420 EM 460/10 (455–465 nm) 170-2421
DNA quantitation (PicoGreen) EX 480/20 (470–490 nm) 170-2427 EM 520/10 (515–525 nm) 170-2424
RNA quantitation (RiboGreen) EX 490/10 (485–495 nm) 170-2422 EM 520/10 (515–525 nm) 170-2424
Oligonucleotide/protein quantitation (OliGreen) EX 480/20 (470–490 nm) 170-2427 EM 520/10 (515–525 nm) 170-2424
Protein quantitation (NanoOrange) EX 480/20 (470–490 nm) 170-2427 EM 590/10 (585–595 nm) 170-2425
Fluorescein EX 490/10 (485–495 nm) 170-2422 EM 520/10 (515–525 nm) 170-2424
Ethidium bromide EX 510/10 (505–515 nm) 170-2423 EM 620/10 (615–625 nm) 170-2426
wtGFP (wild-type Green Fluorescent Protein) EX 390/22 (379–401 nm) 170-2443 EM 510/10 (505–515 nm) 170-2445
EGFP (red-shifted Green Fluorescent Protein) EX 490/10 (485–495 nm) 170-2422 EM 510/10 (505–515 nm) 170-2445
EBFP (blue-emitting Green Fluorescent Protein) EX 380/30 (365–395 nm) 170-2444 EM 440/10 (435–445 nm) 170-2446
GFPuv (UV-excited Green Fluorescent Protein) EX 390/22 (379–401 nm) 170-2443 EM 510/10 (505–515 nm) 170-2445

User-selectable filters support a multitude of applications with a broad range of excitation and emission wavelengths. Custom filter sets are also available from your local Bio-Rad sales representative. This table lists commonly used fluorescent assays and recommended excitation and emission filters.

DNA Quantitation Kit
You can quickly and accurately quantitate dsDNA in solution, even in the presence of RNA or protein, with Bio-Rad's fluorescent DNA quantitation kit. DNA is quantitatively stained with a bis-benzimide dye (Hoechst 33258), which is excited with 346 nm light and emits blue light at 460 nm that is easily detected using a fluorometer such as the VersaFluor fluorometer. The kit quantitates dsDNA in the range of 10 ng/ml to 5 µg/ml and contains reagents for 750 standard 2 ml reactions

FluorAce β-Galactosidase Reporter Assay Kit
You can detect β-galactosidase activity easily and accurately with the FluorAce β-galactosidase reporter assay kit. Simply add the fluorescent substrate β-4-methylumbelliferyl β-D-galactopyranoside (MUG) to the cell or microorganism medium. The substrate penetrates the cell and is hydrolyzed by β-galactosidase, producing the fluorescent compound 4-methylumbelliferone (4MU), which is easily detected using a fluorometer such as the VersaFluor fluorometer. The kit contains reagents for 200 standard 1.5 ml reactions

FluorAce β-Glucuronidase Reporter Assay Kit
You can fluorescently detect β-glucuronidase activity quickly and precisely with the FluorAce β-glucuronidase reporter assay kit. The substrate 4-methylumbelliferyl β-D-glucuronide (MUGluc) is hydrolyzed by β-glucuronidase, producing a fluorogenic compound that is easily detected using a fluorometer such as the VersaFluor fluorometer. The kit contains reagents for 200 standard 1.5 ml reactions

DNA Quantitation Kit

170-2480
Fluorescent assay kit contains Hoechst 33258, calf thymus DNA standard, assay buffer; sufficient for 750 standard 2 ml reactions

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Number Description Options
4100129 Instruction Manual, FluorAce beta-Glucuronidase Reporter Assay Click to download
4100128 Instruction Manual, FluorAce beta-Galactosidase Reporter Assay Kit, Rev A Click to download
4006108 Instruction Manual, CE dsDNA 1000 Fluorescence Detection and Analysis Kit Click to download