ELISA Immuno Explorer Kit

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ELISA Immuno Explorer Kit

Overview

Lab Preparation Checklist
Kit contains sufficient materials for 12 student workstations. (2–4 students per workstation)
Antigen (chicken γ-globulin)* 1
Primary antibody (rabbit anti-chicken polyclonal antibody)* 1
Secondary antibody (goat anti-rabbit antibody conjugated to horseradish peroxidase, or HRP)* 1
HRP enzyme substrate (TMB), 25 ml 1
10x PBS, 100 ml 1
10% Tween 20, 5 ml 1
Disposable plastic transfer pipets 80
Empty 30 ml bottles and caps 3
Microplates with 12-well strips (8 rows of 12 wells) 3
Yellow micro test tubes, 2.0 ml 60
Colored micro test tubes, 2.0 ml 75
Curriculum including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Required Accessories Not Included in Kit:
Adjustable micropipets
    20–200 µl 12
Or fixed volume micropipets
    50 µl 12
Pipet tips
    2–200 µl, BR-35 1 bag
Recommended (Optional) Accessories:
Microplate reader with printer  

* Convenient lyophilized reagents

ELISA Immuno Explorer Kit Flowchart
ELISA Immuno Explorer Kit Flowchart

* Analysis of results: ELISA (enzyme-linked immunosorbent assay) is a highly sensitive and powerful assay that can give qualitative (yes/no) and quantitative (how much?) information. Qualitative results can be determined visually by eye. For precise quantitative determination of concentrations, a microplate reader is required. A detailed lesson extension for quantitative ELISA is included in the kit curriculum.

Key Kit Features
  • Apply a genuine diagnostic procedure
  • Simulate real-world HIV testing
  • Simulate GMO, pregnancy, and drug testing
  • Complete in one 45-minute lab session
Curriculum Fit
ELISA Immuno Explorer Kit Curriculum Fit
How This Kit Works
ELISA plate with positive and negative controls and samples

ELISA plate with positive and negative controls and samples.

How this kit works: Three approaches to ELISA may be taken with this kit. Individual curriculum guides and instructions for each protocol are included in the kit, complete with Teacher’s Guides and Student Manuals. The instructor or the students may select the type of test most relevant to current course work.

1. ELISA for disease outbreaks — tracking the spread of disease.
Step 1: Classroom exchange of simulated body fluids.
Step 2: Follow protocol 2 or 3 below.
Test applications: HIV, bird flu and West Nile viruses, common cold, cholera, smallpox, anthrax, influenza, and STDs

2. ELISA for detecting antigens in a sample.
Test applications: Pregnancy hormones, drug, allergen, and GMO tests; air, food, and water testing; HIV, mad cow disease, smallpox, West Nile, and bird flu viruses

3. ELISA for detecting antibodies in a blood sample for past exposure to a disease.
Test applications: Lyme disease, trichinosis, HIV, West Nile, and bird flu viruses

This kit integrates multiple core content areas and facilitates teaching about immune system functions and about the unique properties of antibodies that have revolutionized medicine, epidemiology, and life science research.

*An antigen is a substance that stimulates an immune response and the production of antibodies. Antigens are usually proteins but can be any type of molecule.

PowerPoint Presentations for Workshops and for Teaching

ELISA Immuno Explorer Kit

These PowerPoint presentations are intended for educational use only. The presentations provide educators with background on the ELISA Immuno Explorer Kit and include instructions for using the kit.

ELISA Immuno Explorer Kit Download ELISA Immuno Explorer Kit, HIV focus PowerPoint presentation

ELISA Immuno Explorer Kit Download ELISA Immuno Explorer Kit, Agriculture focus PowerPoint presentation
ELISA Immuno Explorer Kit

The ELISA Immuno Explorer™ Kit utilizes a technique referred to as enzyme-linked immunosorbent assay (ELISA) to detect the presence of an antibody or an antigen in a sample. The protocol uses two antibodies, one of which is specific to the antigen and another that is coupled to an enzyme. This second antibody gives the assay its enzyme-linked name and causes a chromogenic substrate to produce color wherever it is bound. ELISA is a powerful antibody-based biodetection tool used in the field to hunt for pathogens in water, food, or air, whether they emerge naturally or through acts of aggression.

Features and Benefits

  • Integrates multiple core content areas
  • Facilitates teaching about immune system functions and the unique properties of antibodies that have revolutionized medicine, epidemiology, and life science research

With this kit, students are able to:

  • Perform a genuine diagnostic procedure
  • Simulate real-world HIV, GMO, pregnancy, or drug testing

Applications and Uses

Three approaches to ELISA can be taken with this kit to teach either biodefense, immunology, or health science. Individual curriculum guides and instructions for each protocol are included in the kit, complete with Teachers Guides and Student Manuals.

More Information

  • One 45 min laboratory activity
  • Provides sufficient materials for twelve student workstations, up to four students per workstation

Additional Resources

Download this Zombie Apocalypse activity for a fun way to use the ELISA Immuno Explorer Kit in your classroom

For 12 workstations (2–4 students/workstation)  
Antigen (chicken γ-globulin)* 1
Primary antibody (rabbit anti-chicken polyclonal antibody)* 1
Secondary antibody (goat anti-rabbit antibody, conjugated to horseradish peroxidase, or HRP)* 1
HRP enzyme substrate (TMB), 25 ml 1
10x PBS, 100 ml 1
10% Tween 20, 5 ml 1
Disposable plastic transfer pipets 80
Empty 30 ml bottles and caps 3
Microplates with 12-well strips (8 rows of 12 wells) 3
Yellow micro test tubes, 2.0 ml 60
Colored micro test tubes, 2.0 ml 75
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
*Convenient lyophilized reagents  
ELISA Immuno Explorer Kit

166-2400EDU
Immunology classroom study kit, includes antigen, antibodies, HRP enzyme substrate, 10x PBS, 10% Tween, pipets, test tubes, bottles/caps, microplates, curriculum, for 48 students; education use only

List Price:   $168.75
EDU Price:   $135.00   What is this?
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Number Description Options
3136 ELISA Protocol III (Antibody Test), Biotechnology Explorer, Quick Guide, Rev A Click to download
3135 ELISA Protocol II (Antigen Detection), Biotechnology Explorer, Quick Guide, Rev B Click to download
3134 ELISA Protocol I (Tracking Disease Outbreaks), Biotechnology Explorer, Quick Guide, Rev B Click to download
4110175 Instruction Manual, ELISA Immuno Explorer Kit, Biotechnology Explorer, Rev D Please login to download this document [ Add to Cart (Free) ]