Mini-PROTEAN® Tetra Handcast Systems

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Overview

Maximum Sample Volume per Well for Mini-PROTEAN® Tetra Cell Combs
  Comb Thickness
Number or Type of Wells Well Width 0.75 mm 1.0 mm 1.5 mm
5 12.70 mm 70 µl 105 µl 160 µl
9 5.08 mm 33 µl 44 µl 66 µl
10 5.08 mm 33 µl 44 µl 66 µl
15 3.35 mm 20 µl 26 µl 40 µl
IPG 6.20 mm 420 µl 730 µl
Prep/2-D
       
   Reference well 3.10 mm 13 µl 17 µl 30 µl
   Sample well 67.44 mm 310 µl 400 µl 680 µl
 
Specifications      
Number of gels 1–4 Total buffer volume for 2 gels 700 ml
Precast gels Mini-PROTEAN and Ready Gel Total buffer volume for 4 gels 1,000 ml
Handcast gels Cast using Mini-PROTEAN spacer plates Typical run times for SDS-PAGE 35–45 min (at 200 V constant)
Cassette size (W x L) Precast: 10 x 8.3 cm Recommended power supply PowerPac™ Basic or PowerPac HC
Glass plate size (W x L) Short plate: 10.1 x 7.3 cm Dimensions (W x L x H) 12 x 16 x 18 cm
  Spacer plate: 10.1 x 8.2 cm Weight 1 kg (2.2 lb)

The Mini-PROTEAN® Tetra handcast systems are based on the 4-gel Mini-PROTEAN Tetra cell or the high-throughput Mini-PROTEAN® 3 Dodeca™ cell (for running up to 12 gels). The accessories for hand casting provide options for casting mini gels in a variety of thicknesses and comb configurations, and even offer handy gel stands for help in casting gels.

Combs

A variety of comb styles are available, including 5-, 9-, 10-, and 15-well formats. The prep/2-D comb contains a reference well and the IPG comb is designed to accommodate a 7 cm ReadyStrip™ IPG strip. These combs fit both Mini-PROTEAN Tetra and Mini-PROTEAN 3 cells.

Plates for Hand Casting

To hand cast a gel, you need a short plate and a spacer plate. You do not need to order spacers separately; simply select the spacer plate that has the spacer width you need (spacer plates contain spacers already joined to the plate). The short plates and the spacer plates fit both Mini-PROTEAN Tetra and Mini-PROTEAN 3 cells.

Mini-PROTEAN 3 Multi-Casting Chamber

Use the Mini-PROTEAN 3 multi-casting chamber to simultaneously cast up to 12 gels of 0.75, 1.0, or 1.5 mm thickness. Acrylic blocks act as space fillers when fewer than 12 gels are cast. You can cast gradient gels through a bottom-filling port with the Model 485 gradient former to ensure reproducibility. Gels cast in the multi-casting chamber can be run on any of the Mini-PROTEAN electrophoresis systems, including the Mini-PROTEAN Tetra cell.

Mini-PROTEAN Tetra Cell with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Cell

165-8000FC
4-gel vertical electrophoresis system, 0.75 mm gel thickness, includes companion module, 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

List Price:   $897.00
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Mini-PROTEAN Tetra Cell with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Cell

165-8001FC
4-gel vertical electrophoresis system, 1.0 mm gel thickness, includes companion module, 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

List Price:   $897.00
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Mini-PROTEAN® Tetra Cell

165-8002FC
2-gel vertical electrophoresis system, 0.75 mm gel thickness, includes casting stand, 2 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

List Price:   $708.00
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Mini-PROTEAN Tetra Cell with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Cell

165-8003FC
2-gel vertical electrophoresis system, 1.0 mm gel thickness, includes casting stand, 2 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

List Price:   $708.00
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Mini-PROTEAN Tetra Cell with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Cell

165-8006FC
4-gel vertical electrophoresis system, 1.5 mm gel thickness, includes companion module, 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

List Price:   $897.00
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Mini-PROTEAN Tetra Cell with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Cell

165-8007FC
2-gel vertical electrophoresis system, 1.5 mm gel thickness, includes 1 casting stand, 2 casting frames, 10-well combs, 5 sets of glass plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

List Price:   $708.00
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Mini-PROTEAN Tetra Cell and PowerPac Basic Power Supply with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Cell and PowerPac™ Basic Power Supply

165-8025FC
4-gel vertical electrophoresis system (#165-8001) and PowerPac Basic Power Supply (#164-5050), for 1.0 mm gels, with five 10-well combs and casting accessories for up to 4 gels and TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

List Price:   $1,175.00
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Mini-PROTEAN Tetra Cell and PowerPac Universal Power Supply with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Cell and PowerPac™ Universal Power Supply

165-8026FC
4-gel vertical electrophoresis system (#165-8001) and PowerPac Universal Power Supply (#164-5070), for 1.0 mm gels, includes five 10-well combs and casting accessories for up to 4 gels, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

List Price:   $2,971.00
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Mini-PROTEAN Tetra Cell and PowerPac HC Power Supply with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Cell and PowerPac™ HC Power Supply

165-8027FC
4-gel vertical electrophoresis system (#165-8001) and PowerPac High-Current Power Supply (#164-5052), for 1.0 mm gels, includes five 10-well combs and casting accessories for up to 4 gels, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

List Price:   $1,615.00
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Mini-PROTEAN Tetra Cell and PowerPac HV Power Supply with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Cell and PowerPac™ HV Power Supply

165-8028FC
4-gel vertical electrophoresis system (#165-8001) and PowerPac High-Voltage Power Supply (#164-5056), for 1.0 mm gels, includes five 10-well combs and casting accessories for up to 4 gels, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

List Price:   $2,919.00
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Mini-PROTEAN Tetra Cell and Mini Trans-Blot Module with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Cell and Mini Trans-Blot® Module

165-8029FC
Electrophoresis and blotting system, 10-well, 1.0 mm, 4-gel system (#165-8001) and blotting module (#170-3935) without lower buffer tank and lid, includes gel casting accessories, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

List Price:   $1,228.00
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Mini-PROTEAN Tetra Cell, Mini Trans-Blot Module, and PowerPac Basic Power Supply with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Cell with Mini Trans-Blot® Module, and PowerPac™ Basic Power Supply

165-8033FC
Electrophoresis and blotting system, 10-well, 1.0 mm, 4-gel system (#165-8001) and blotting module (#170-3935) without lower buffer tank and lid, with PowerPac Basic Power Supply (#164-5050) and TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

List Price:   $1,455.00
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Mini-PROTEAN Tetra Cell, Mini Trans-Blot Module, and PowerPac HC Power Supply with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Cell with Mini Trans-Blot® Module and PowerPac™ HC Power Supply

165-8035FC
Electrophoresis and blotting system, 10-well, 1.0 mm, 4-gel system (#165-8001), blotting module (#170-3935) without lower buffer tank and lid, includes PowerPac High-Current Power Supply (#164-5052) and TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

List Price:   $1,980.00
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Mini-PROTEAN 3 Multi-Casting Chamber

165-4111
Pkg of 1, polyacrylamide gel casting chamber with 0.75 mm spacers, includes 15 sets of glass plates; order combs separately

List Price:   $550.00
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Mini-PROTEAN 3 Multi-Casting Chamber

165-4112
Pkg of 1, polyacrylamide gel casting chamber with 1.0 mm spacers, includes 15 sets of glass plates; order combs separately

List Price:   $550.00
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Mini-PROTEAN 3 Multi-Casting Chamber

165-4113
Pkg of 1, polyacrylamide gel casting chamber with 1.5 mm spacers, includes 15 sets of glass plates; order combs separately

List Price:   $550.00
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Acrylic Blocks

165-4114
Pkg of 8, 6 mm space filler, for use with Mini-PROTEAN 3 multi-casting chamber (#165-4110)

List Price:   $92.00
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Separation Sheets

165-4115
Pkg of 15, reusable sheet for separation of gel sandwiches after casting in the Mini-PROTEAN 3 multi-casting chamber (#165-4110)

List Price:   $58.00
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Model 485 Gradient Former

165-4120
40–175 ml capacity acrylamide gradient former, includes body with value stem and tubing connection kit

List Price:   $784.00
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Model 485 Gradient Former and Mini-PROTEAN 3 Multi-Casting Chamber

165-4122
Gradient gel casting system, includes 40–175 ml acrylamide gradient former (#165-4120) and 12-gel casting chamber (#165-4110) with accessories

List Price:   $1,127.00
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Exponential Piston

165-2006
Pkg of 1, exponential piston for Model 385 and Model 485 gradient formers

List Price:   $276.00
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AnyGel™ Stand

165-4131
Pkg of 1, single-row gel stand, holds 1 PROTEAN® gel, 2 Criterion™ gels, or 3 Mini-PROTEAN® or Ready Gel® precast mini gels

List Price:   $73.00
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AnyGel™ Stand

165-5131
Pkg of 1, 6-row gel stand, holds 6 PROTEAN® gels, 12 Criterion™ gels, or 18 Mini-PROTEAN® or Ready Gel® precast mini gels

List Price:   $271.00
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Number Description Options
10007296 Instruction Manual, Mini-PROTEAN Tetra Cell, Rev D Click to download
10024259 Mini-PROTEAN Empty Cassette Gel Casting Instructions, Rev A Click to download
M4006192 Instruction Manual, Model 485 Gradient Former, Rev A Click to download
M4006193 Instruction Manual, Mini-PROTEAN 3 Multi-Casting Chamber, Rev A Click to download
M1652000 Instruction Manual, Model 385 and Model 395 Gradient Formers, Rev C (discontinued product, PDF only) Click to download