Mini-PROTEAN® Tetra Handcast Systems

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Overview

Maximum Sample Volume per Well for Mini-PROTEAN® Tetra Cell Combs
  Comb Thickness
Number or Type of Wells Well Width 0.75 mm 1.0 mm 1.5 mm
5 12.70 mm 70 µl 105 µl 160 µl
9 5.08 mm 33 µl 44 µl 66 µl
10 5.08 mm 33 µl 44 µl 66 µl
15 3.35 mm 20 µl 26 µl 40 µl
IPG 6.20 mm 420 µl 730 µl
Prep/2-D
       
   Reference well 3.10 mm 13 µl 17 µl 30 µl
   Sample well 67.44 mm 310 µl 400 µl 680 µl
 
Specifications      
Number of gels 1–4 Total buffer volume for 2 gels 700 ml
Precast gels Mini-PROTEAN and Ready Gel Total buffer volume for 4 gels 1,000 ml
Handcast gels Cast using Mini-PROTEAN spacer plates Typical run times for SDS-PAGE 35–45 min (at 200 V constant)
Cassette size (W x L) Precast: 10 x 8.3 cm Recommended power supply PowerPac™ Basic or PowerPac HC
Glass plate size (W x L) Short plate: 10.1 x 7.3 cm Dimensions (W x L x H) 12 x 16 x 18 cm
  Spacer plate: 10.1 x 8.2 cm Weight 1 kg (2.2 lb)

The Mini-PROTEAN® Tetra handcast systems are based on the 4-gel Mini-PROTEAN Tetra cell or the high-throughput Mini-PROTEAN® 3 Dodeca™ cell (for running up to 12 gels). The accessories for hand casting provide options for casting mini gels in a variety of thicknesses and comb configurations, and even offer handy gel stands for help in casting gels.

Combs

A variety of comb styles are available, including 5-, 9-, 10-, and 15-well formats. The prep/2-D comb contains a reference well and the IPG comb is designed to accommodate a 7 cm ReadyStrip™ IPG strip. These combs fit both Mini-PROTEAN Tetra and Mini-PROTEAN 3 cells.

Plates for Hand Casting

To hand cast a gel, you need a short plate and a spacer plate. You do not need to order spacers separately; simply select the spacer plate that has the spacer width you need (spacer plates contain spacers already joined to the plate). The short plates and the spacer plates fit both Mini-PROTEAN Tetra and Mini-PROTEAN 3 cells.

Mini-PROTEAN 3 Multi-Casting Chamber

Use the Mini-PROTEAN 3 multi-casting chamber to simultaneously cast up to 12 gels of 0.75, 1.0, or 1.5 mm thickness. Acrylic blocks act as space fillers when fewer than 12 gels are cast. You can cast gradient gels through a bottom-filling port with the Model 485 gradient former to ensure reproducibility. Gels cast in the multi-casting chamber can be run on any of the Mini-PROTEAN electrophoresis systems, including the Mini-PROTEAN Tetra cell.

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Mini-PROTEAN Tetra Cell with FastCast Acrylamide Kit
Mini-PROTEAN® Vertical Electrophoresis Cell, 4-gel, for 0.75 mm thick handcast gels

1658000FC
4-gel vertical electrophoresis system, 0.75 mm gel thickness, includes companion module, 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

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$905.00
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Mini-PROTEAN Tetra Cell with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Vertical Electrophoresis Cell, 4-gel, for 1.0 mm thick handcast gels

1658001FC
4-gel vertical electrophoresis system, 1.0 mm gel thickness, includes companion module, 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

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$905.00
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Mini-PROTEAN® Tetra Vertical Electrophoresis Cell, 2-gel, for 0.75 mm thick handcast gels

1658002FC
2-gel vertical electrophoresis system, 0.75 mm gel thickness, includes casting stand, 2 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

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$715.00
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Mini-PROTEAN Tetra Cell with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Vertical Electrophoresis Cell, 2-gel, for 1.0 mm thick handcast gels

1658003FC
2-gel vertical electrophoresis system, 1.0 mm gel thickness, includes casting stand, 2 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

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$715.00
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Mini-PROTEAN Tetra Cell with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Vertical Electrophoresis Cell, 4-gel, for 1.5 mm thick handcast gels

1658006FC
4-gel vertical electrophoresis system, 1.5 mm gel thickness, includes companion module, 2 casting stands, 4 casting frames, 10-well combs, 5 short plates, and 5 spacer plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

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$905.00
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Mini-PROTEAN Tetra Cell with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Vertical Electrophoresis Cell, 2-gel, for 1.5 mm thick handcast gels

1658007FC
2-gel vertical electrophoresis system, 1.5 mm gel thickness, includes 1 casting stand, 2 casting frames, 10-well combs, 5 sets of glass plates, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

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$715.00
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Mini-PROTEAN Tetra Cell and PowerPac Basic Power Supply with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Vertical Electrophoresis Cell, 4-gel, for 1.0 mm thick handcast gels, with PowerPac™ Basic Power Supply

1658025FC
4-gel vertical electrophoresis system (#165-8001) and PowerPac Basic Power Supply (#164-5050), for 1.0 mm gels, with five 10-well combs and casting accessories for up to 4 gels and TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

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$1,190.00
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Mini-PROTEAN Tetra Cell and PowerPac Universal Power Supply with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Vertical Electrophoresis Cell, 4-gel, for 1.0 mm thick handcast gels, with PowerPac™ Universal Power Supply

1658026FC
4-gel vertical electrophoresis system (#165-8001) and PowerPac Universal Power Supply (#164-5070), for 1.0 mm gels, includes five 10-well combs and casting accessories for up to 4 gels, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

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$3,000.00
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Mini-PROTEAN Tetra Cell and PowerPac HC Power Supply with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Vertical Electrophoresis Cell, 4-gel, for 1.0 mm thick handcast gels, with PowerPac™ HC Power Supply

1658027FC
4-gel vertical electrophoresis system (#165-8001) and PowerPac High-Current Power Supply (#164-5052), for 1.0 mm gels, includes five 10-well combs and casting accessories for up to 4 gels, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

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$1,635.00
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Mini-PROTEAN Tetra Cell and PowerPac HV Power Supply with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Vertical Electrophoresis Cell, 4-gel, for 1.0 mm thick handcast gels, with PowerPac™ HV Power Supply

1658028FC
4-gel vertical electrophoresis system (#165-8001) and PowerPac High-Voltage Power Supply (#164-5056), for 1.0 mm gels, includes five 10-well combs and casting accessories for up to 4 gels, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

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$2,950.00
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Mini-PROTEAN Tetra Cell and Mini Trans-Blot Module with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Vertical Electrophoresis Cell, 4-gel, for 1.0 mm thick handcast gels, with Mini Trans-Blot® Module

1658029FC
Electrophoresis and blotting system, 10-well, 1.0 mm, 4-gel system (#165-8001) and blotting module (#170-3935) without lower buffer tank and lid, includes gel casting accessories, with TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

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$1,240.00
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Mini-PROTEAN Tetra Cell, Mini Trans-Blot Module, and PowerPac Basic Power Supply with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Vertical Electrophoresis Cell, 4-gel, for 1.0 mm thick handcast gels, with Mini Trans-Blot® Module and PowerPac™ Basic Power Supply

1658033FC
Electrophoresis and blotting system, 10-well, 1.0 mm, 4-gel system (#165-8001) and blotting module (#170-3935) without lower buffer tank and lid, with PowerPac Basic Power Supply (#164-5050) and TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

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$1,470.00
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Mini-PROTEAN Tetra Cell, Mini Trans-Blot Module, and PowerPac HC Power Supply with FastCast Acrylamide Kit
Mini-PROTEAN® Tetra Vertical Electrophoresis Cell, 4-gel, for 1.0 mm thick handcast gels, with Mini Trans-Blot® Module and PowerPac™ HC Power Supply

1658035FC
Electrophoresis and blotting system, 10-well, 1.0 mm, 4-gel system (#165-8001), blotting module (#170-3935) without lower buffer tank and lid, includes PowerPac High-Current Power Supply (#164-5052) and TGX Stain-Free™ FastCast™ Acrylamide Starter Kit, 10% (161-0182)

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$2,000.00
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Mini-PROTEAN® 3 Multi-Casting Chamber

1654111
Pkg of 1, polyacrylamide gel casting chamber with 0.75 mm spacers, includes 15 sets of glass plates; order combs separately

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$580.00
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Mini-PROTEAN® 3 Multi-Casting Chamber

1654112
Pkg of 1, polyacrylamide gel casting chamber with 1.0 mm spacers, includes 15 sets of glass plates; order combs separately

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$580.00
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Mini-PROTEAN® 3 Multi-Casting Chamber

1654113
Pkg of 1, polyacrylamide gel casting chamber with 1.5 mm spacers, includes 15 sets of glass plates; order combs separately

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$580.00
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Acrylic Blocks

1654114
Pkg of 8, 6 mm space filler, for use with Mini-PROTEAN® 3 multi-casting chamber (#165-4110)

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$92.00
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Separation Sheets

1654115
Pkg of 15, reusable sheet for separation of gel sandwiches after casting in the Mini-PROTEAN® 3 multi-casting chamber (#165-4110)

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$58.00
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Model 485 Gradient Former

1654120
40–175 ml capacity acrylamide gradient former, includes body with value stem and tubing connection kit

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$825.00
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Model 485 Gradient Former and Mini-PROTEAN® 3 Multi-Casting Chamber

1654122
Gradient gel casting system, includes 40–175 ml acrylamide gradient former (#165-4120) and 12-gel casting chamber (#165-4110) with accessories

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$1,185.00
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Exponential Piston

1652006
Pkg of 1, exponential piston for Model 385 and Model 485 gradient formers

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$290.00
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AnyGel™ Stand

1654131
Pkg of 1, single-row gel stand, holds 1 PROTEAN® gel, 2 Criterion™ gels, or 3 Mini-PROTEAN® or Ready Gel® precast mini gels

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$76.00
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AnyGel™ Stand

1655131
Pkg of 1, 6-row gel stand, holds 6 PROTEAN® gels, 12 Criterion™ gels, or 18 Mini-PROTEAN® or Ready Gel® precast mini gels

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$285.00
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Number Description Options
10007296 Instruction Manual, Mini-PROTEAN® Tetra Cell, Rev D Click to download
10024259 Mini-PROTEAN Empty Cassette Gel Casting Instructions, Rev A Click to download
M4006192 Instruction Manual, Model 485 Gradient Former, Rev A Click to download
M4006193 Instruction Manual, Mini-PROTEAN 3 Multi-Casting Chamber, Rev A Click to download
M1652000 Instruction Manual, Model 385 and Model 395 Gradient Formers, Rev C (discontinued product, PDF only) Click to download
5535 Mini-PROTEAN® Electrophoresis System Brochure, Rev F Click to download [ Add to Cart (Free) ]