Trans-Blot® Turbo™ Transfer System

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Overview

Superior Transfer Efficiency

Higher sensitivity and better transfer efficiency is seen with the Trans-Blot® Turbo™ system in comparison to other blotting techniques. This data set demonstrates the successful transfer of the 1.25 ng protein band only with the Trans-Blot Turbo system. Serial dilutions of transferrin were separated on a Criterion™ TGX™ 4–20% gel and transferred using four different blotting techniques. All images are adjusted to the same settings.




Trans-Blot® Turbo™ System Tour
Trans-Blot® Turbo™ System Instructional Video


Instructional video to guide you through the features, benefits, and operation of the Trans-Blot Turbo system.

Trans-Blot® Turbo™ System vs. Traditional Blotting Techniques


See how the Trans-Blot Turbo system measures up against traditional blotting techniques.

Trans-Blot® Turbo™ System vs. iBlot


See how the Trans-Blot Turbo system performs compared to the iBlot dry blotting system.

Transfer in 3, 7, or 10 Min

Turbo Transfers with Trans-Blot® Turbo™ Transfer Packs

  • 3-minute protocol — a single Mini-PROTEAN® TGX™ gel (for proteins with MW 5–150 kD) can be transferred in as little as 3 min
  • 7-minute protocol — up to 4 mini or 2 midi gels with mixed-molecular weight proteins (MW 5–150 kD) can be efficiently transferred in 7 min
  • 10-minute protocol — up to 4 mini or 2 midi gels with high-molecular weight proteins (MW 25–300+ kD) can be efficiently transferred in 10 min

E. coli lysate (6 µg) was diluted two-fold. Samples were separated with Mini-PROTEAN TGX gels, transferred with the Trans-Blot Turbo system, stained with SYPRO Ruby and imaged on a VersaDoc™ MP 4000 system. Standards in lane 1 are Precision Plus Protein™ Unstained standards, with a top band of 250 kD MW.


Better Transfer of Different Types of Proteins

2x Protein Transfer

Quantitation performed on equivalent 2-D gels transferred with the Trans-Blot® Turbo™ and the iBlot systems demonstrated twice the number of proteins transferred and detected with the Trans-Blot Turbo system. Rat liver extract was focused on Bio-Rad's ReadyStrip™ 11 cm pH 5–8 IPG strips and separated on AnykD™ Criterion™ TGX™ gels. Duplicate gels were transferred with the Trans-Blot Turbo and iBlot systems, both using manufacturers’ recommended 7 min protocol. The nitrocellulose membranes were subsequently stained with SYPRO Ruby and imaged on a VersaDoc™ MP 4000 system.


Blotter Comparison
 
  Tank Semi-dry iBlot Trans-Blot® Turbo™ system
Speed of transfer •• ••• ••••
Flexibility •• •• •••
Transfer efficiency even for high MW ••• •• •••
Throughput 2 mini gels 6 mini/3 midi gels 2 mini/1 midi gel 4 mini/2 midi gels
Power requirement External power supply (PowerPac™ HC) External power supply (PowerPac™ HC) Integrated power supply Integrated power supply
User interface None None Preset protocols only Preset protocols and easy customization

The Trans-Blot Turbo transfer system combines traditional blotting techniques with modern filter paper and buffers, allowing rapid transfer of proteins from gels to membranes with minimal preparation time. The system integrates a high amperage power supply that directs current between a built-in platinized titanium anode and a stainless steel cathode. This enables highly efficient and rapid transfer in as little as 3 minutes. The entire system is in a ready-to-use format that allows researchers to obtain their results faster and easier, with reproducibility that is difficult to achieve using traditional blotting methods.

  • Rapid transfer — transfers mini or midi gels in as little as 3 min
  • High throughput — can transfer 1–4 mini or 1–2 midi gels in a single run
  • Greater transfer efficiency — higher transfer efficiency compared to other transfer methods
  • Flexible design — allows user to customize transfer conditions and is compatible with traditional semi-dry consumables
  • Environmentally friendly — environmentally safe consumables eliminate disposal cost
Trans-Blot® Turbo™ Transfer Starter System

170-4155EDU
Blotting instrument, includes base, 2 cassettes to hold 1–2 midi or up to 4 mini blotting sandwiches, blot roller, and starter consumable kit; education use only

List Price:   $2,995.00
EDU Price:   $2,396.00   What is this?
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Trans-Blot® Turbo™ Mini PVDF Transfer Packs

170-4156EDU
Pkg of 10, 7 x 8.5 cm, precut blotting transfer pack, includes filter paper, buffer, PVDF membrane, for use with Trans-Blot Turbo transfer system; education use only

List Price:   $164.00
EDU Price:   $131.20   What is this?
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Trans-Blot® Turbo™ Midi PVDF Transfer Packs

170-4157EDU
Pkg of 10, 8.5 x 13.5 cm, precut blotting transfer pack, includes filter paper, buffer, PVDF membrane, for use with Trans-Blot Turbo transfer system; education use only

List Price:   $194.00
EDU Price:   $155.20   What is this?
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Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs

170-4158EDU
Pkg of 10, 7 x 8.5 cm, precut blotting transfer pack, includes filter paper, buffer, nitrocellulose membrane, for use with Trans-Blot Turbo transfer system; education use only

List Price:   $143.00
EDU Price:   $114.40   What is this?
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Trans-Blot® Turbo™ Midi Nitrocellulose Transfer Packs

170-4159EDU
Pkg of 10, 8.5 x 13.5 cm, precut blotting transfer pack, includes filter paper, buffer, nitrocellulose membrane, for use with Trans-Blot Turbo transfer system; education use only

List Price:   $174.00
EDU Price:   $139.20   What is this?
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Rapid Blotting and V3 Western Workflow™ Starter Kit

166-2875EDU
Comprehensive protein classroom study kit, includes Protein Profiler Module (#166-2700EDU), Western Blot Module (#166-2800EDU), Trans-Blot® Turbo™ mini nitrocellulose transfer packs (10), 4–20% Mini-PROTEAN® TGX Stain-Free™ gels (10), application note, for 32 students; education use only

List Price:   $700.00
EDU Price:   $560.00   What is this?
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Number Description Options
M1662876EDU Biotechnology Explorer Rapid Blotting + V3 Western Workflow (Stain-Free Rapid Blotting) Application Note, Rev A Click to download
2895 Protein Blotting Guide, Interactive PDF, Rev B Click to download
6039 Trans-Blot Turbo Transfer System Brochure, Rev D Click to download [ Add to Cart (Free) ]
6088 V3 Western Workflow Brochure with Gel Doc EZ Imager, Rev B Click to download [ Add to Cart (Free) ]
10020688 Instruction Manual, Trans-Blot Turbo Blotting System, Rev F Click to download
10016505 Instruction Manual, Trans-Blot Turbo Transfer System Quick Start Guide, Rev D Click to download [ Add to Cart (Free) ]
10019593 Instruction Manual, Trans-Blot Turbo Transfer System Transfer Pack Quick Start Guide, Rev E Click to download [ Add to Cart (Free) ]
RP0051 Stain-Free Approach for Western Blotting Click to download
6148 Transfer of High Molecular Weight Proteins to Membranes: A Comparison of Transfer Efficiency Between Blotting Systems, Rev B Click to download [ Add to Cart (Free) ]
6147 Robust Transfer of Proteins to Membranes Across a Wide Range of pI and Mass Using the Trans-Blot Turbo System, Rev B Click to download [ Add to Cart (Free) ]