Econo-Pac® Protein A Prepacked Gravity Flow Columns

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Overview

Specifications
Product
Description
Packed
Support
Bed
Volume
Particle Size (µm) Capacity Applications
Econo-Pac®
protein A
column
Affi-Gel®
protein A gel
2 ml 10–14 mg
mouse IgG1*
10–14 mg
mouse IgG1*
Gravity-flow purification of monoclonal antibodies from small amounts of ascites fluid or serum.
Econo-Pac
protein A kit
300mg mouse
IgG1*
Contains everything necessary to purify monoclonal anitbodies from ascites fluid or serum, including Affi-Gel protein A column, reusable Econo-Pac 10DG columns (for a sample preparation and buffer exchange of the purified antibody), and MAPS II buffers. Enough reagents to purify 500 mg mouse IgG1.
Econo-Pac
serum IgG
purification
column
DEAE Affi-Gel
blue gel
10 ml 75–150 3 ml serum per column per run For purification of IgG from other serum proteins and plasminogen with only residual transferrin contamination. Columns can be resued 8–10 times.
Econo-Pac
serum IgG
purification
3 ml serum per column Contains everything necessary to purify IgG from serum, including 5 Econo-Pac serum IgG purification columns, reusable Econo-Pac 10DG column for sample preparation, and premixed buffers for human and rabbit IgG applications. Up to 15 ml serum can be purified at one time.
Econo-Pac
10DG desalting gel column
Bio-Gel® P-6 gel 10 ml 90–180 3 ml sample
per column per run
Gravity-flow column for desalting and buffer exchange applications. Exclusion limit is 6,000 Da.

* When used with MAPS II buffers.

Prepacked Cartridge Selection by Application
Cartridge by Application Support Type of Separation Chemical Form Protein capacity/ml*
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange -N+(CH3)3 ≥37 mg BSA
UNOsphere Q UNOsphere™ Q Strong anion exchange -N+(CH3)3 180 mg BSA
UNOsphere S UNOsphere S Strong cation exchange -SO3- 60 mg human IgG
DEAE Macro-Prep DEAE Weak anion exchange -HN+(C2H5)2 >35 mg protein
Protein Purification
High S Macro-Prep High S Strong cation exchange -SO3- ≥49 mg human IgG
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity Cibacron Blue
F3GA and DEAE
0.2–1.0 ml serum
Antibody Purification
Protein A Affi-Prep® protein A Affinity Protein A 8–10 mg mouse monoclonal IgG1
CHT™ Type I CHT ceramic
hydroxyapatite Type I
Hydroxyapatite (Ca5(PO4)3OH)2 16–23 mg human IgG
CHT Type II CHT ceramic
hydroxyapatite Type II
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human IgG
Affinity-Tagged Protein Purification
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
His-tagged protein
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV***
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 mlm serum
11 mg albumin

*   Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
**  IMAC and GST cartridges are designed for use with the Profinia protein purification system.
*** Column volume.

Product Selection by Technique
   
Scale
Packaging Format
Characteristics Product Type Analytical Pilot/Preparative Process Prepacked Bottled
Ion Exchange Chromatography
Separation by charge UNOsphere™ media     UNOsphere Q, S, Rapid S
General biomolecules purification Macro-Prep® media     Macro-Prep High Q, DEAE, High S, CM
Applicable to all antibody purifications Macro-Prep 25 media     Macro-Prep 25 Q, 25 S
UNO® columns   UNO Q, S  
Bio-Scale™ Mini Cartridges     UNOsphere Q, S  
High capacity         Macro-Prep High Q, S  
Good capture technique AG® resins   AG
Chelex® resins Chelex
Bio-Rex® resins Bio-Rex
Hydroxyapatite and Fluoroapatite Chromatography
Unique selectivity CHT™ 80 µm support     CHT, Types I and II
Alternative to protein A for antibody purification CHT 40 µm support     CHT, Types I and II
CHT 20 µm support   CHT, Types I and II
CFT 40 µm support CFT, Types I and II
Removal of leached protein A, aggregates, endotoxins, and DNA Bio-Gel® HT and HTP media     Bio-Gel HT, HTP
Bio-Scale columns   CHT Type I (10 µm)  
CHT Type II (20 µm)  
Affinity Chromatography
Purification of His- and GST- tagged proteins Profinity™ IMAC and GST media   Bio-Scale Mini Profinity IMAC
Separation by biological interaction Affi-Gel resins     Affi-Gel® Protein A*
Affi-Gel Blue*
DEAE Affi-Gel Blue*
CM Affi-Gel Blue*
High selectivity           Affi-Gel boronate, heparin**
High purity in a single step           Affi-Gel Hz, 10, 15**
Affi-Gel 102**
Excellent capture technique Profinity epoxide media     Profinity epoxide**
Affi-Prep media   Affi-Prep protein A*
Affi-Prep polymyxin*
Bio-Scale Mini cartridges   Profinity IMAC and GST  
Econo-Pac® columns   Affi-Gel Blue  
DEAE Affi-Gel Blue
Affi-Gel protein A
Size Exclusion Chromatography
Separation by size Bio-Gel P media     Bio-Gel P
MW determination Bio-Beads™ S-X media     Bio-Beads S-X
Applicable to all antibody purifications Bio-Sil® and Bio-Silect™ columns     Bio-Sil, Bio-Silect  
Desalting or buffer exchange Bio-Scale Mini cartridges     Bio-Gel P-6  
High resolution Econo-Pac columns     Econo-Pac 10DG  
Hydrophobic Interaction Chromatography
Separation by hydrophobicity Macro-Prep HIC media     Macro-Prep t-butly
Macro-Prep methyl

* Ready to use.
** Activated.



Econo-Pac prepacked columns for gravity-flow chromatography allow fast and simplified antibody purification. The prepacked Econo-Pac columns include an upper frit, a snap-off end tip, graduated column markings, and a 30 ml total column volume. To improve column performance, use a flow adaptor.

Econo-Pac Protein A Columns

732-2022
Pkg of 5, prepacked gravity-flow chromatography columns, prefilled with Affi-Gel protein A gel

List Price:   $507.00
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Econo-Pac® Protein A Kit

732-2020
Purification kit, includes Affi-Gel® protein A column, four 10DG desalting columns, buffers

List Price:   $307.00
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Hazardous  
Econo-Pac Serum IgG Purification Kit

732-2027
Antibody purification kit, includes 5 x 5 ml DEAE Affi-Gel Blue columns, 1 x 10 ml 10DG column, buffers

List Price:   $379.00
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Number Description Options
LIT154 Instruction Manual, Econo-Pac Serum IgG Purification Kit and Econo-Pac Serum IgG Purification Columns, Rev B Click to download
LIT153 Instruction Manual, Econo-Pac Protein A Kit, Protein A Columns, Rev B Click to download [ Add to Cart (Free) ]