Econo-Pac® 10DG Desalting Prepacked Gravity Flow Columns

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Overview

Specifications
Product
Description
Packed
Support
Bed
Volume
Particle Size (µm) Capacity Applications
Econo-Pac®
protein A
column
Affi-Gel®
protein A gel
2 ml 10–14 mg
mouse IgG1*
10–14 mg
mouse IgG1*
Gravity-flow purification of monoclonal antibodies from small amounts of ascites fluid or serum.
Econo-Pac
protein A kit
300mg mouse
IgG1*
Contains everything necessary to purify monoclonal anitbodies from ascites fluid or serum, including Affi-Gel protein A column, reusable Econo-Pac 10DG columns (for a sample preparation and buffer exchange of the purified antibody), and MAPS II buffers. Enough reagents to purify 500 mg mouse IgG1.
Econo-Pac
serum IgG
purification
column
DEAE Affi-Gel
blue gel
10 ml 75–150 3 ml serum per column per run For purification of IgG from other serum proteins and plasminogen with only residual transferrin contamination. Columns can be resued 8–10 times.
Econo-Pac
serum IgG
purification
3 ml serum per column Contains everything necessary to purify IgG from serum, including 5 Econo-Pac serum IgG purification columns, reusable Econo-Pac 10DG column for sample preparation, and premixed buffers for human and rabbit IgG applications. Up to 15 ml serum can be purified at one time.
Econo-Pac
10DG desalting gel column
Bio-Gel® P-6 gel 10 ml 90–180 3 ml sample
per column per run
Gravity-flow column for desalting and buffer exchange applications. Exclusion limit is 6,000 Da.

* When used with MAPS II buffers.

Prepacked Cartridge Selection by Application
Cartridge by Application Support Type of Separation Chemical Form Protein capacity/ml*
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange -N+(CH3)3 ≥37 mg BSA
UNOsphere Q UNOsphere™ Q Strong anion exchange -N+(CH3)3 180 mg BSA
UNOsphere S UNOsphere S Strong cation exchange -SO3- 60 mg human IgG
DEAE Macro-Prep DEAE Weak anion exchange -HN+(C2H5)2 >35 mg protein
Protein Purification
High S Macro-Prep High S Strong cation exchange -SO3- ≥49 mg human IgG
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity Cibacron Blue
F3GA and DEAE
0.2–1.0 ml serum
Antibody Purification
Protein A Affi-Prep® protein A Affinity Protein A 8–10 mg mouse monoclonal IgG1
CHT™ Type I CHT ceramic
hydroxyapatite Type I
Hydroxyapatite (Ca5(PO4)3OH)2 16–23 mg human IgG
CHT Type II CHT ceramic
hydroxyapatite Type II
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human IgG
Affinity-Tagged Protein Purification
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
His-tagged protein
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV***
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 mlm serum
11 mg albumin

*   Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
**  IMAC and GST cartridges are designed for use with the Profinia protein purification system.
*** Column volume.

Column and Resin Selection Guide

Column and Resin Selection Guide Capture Column and Resin Selection Guide Intermediate Purification Column and Resin Selection Guide Polishing

Econo-Pac 10DG Desalting Columns for gravity-flow chromatography allow fast and simplified antibody purification. These columns are prepacked with Bio-Gel® P-6DG Gel and include an upper frit, a snap-off end tip, graduated column markings, and a 30 ml total column volume. To improve column performance at higher flow rates, use an Econo-Pac Flow Adaptor (738-0019).

Econo-Pac 10DG Desalting Columns

732-2010
Pkg of 30, 10 ml packed resin, 20 ml reservoir, prepacked gravity-flow chromatography column, includes upper frit

List Price:   $189.00
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Number Description Options
LIT81 Instruction Manual, Econo-Pac 10DG Columns Click to download [ Add to Cart (Free) ]