The QX100 Droplet Digital PCR (ddPCR™) system provides an absolute quantification of target DNA molecules with unrivaled performance in precision, accuracy, and sensitivity for quantitative PCR applications. The award-winning QX100 Droplet Digital PCR system is the third generation of PCR technology and provides a revolutionary approach to target DNA quantification.
The QX100 Droplet Digital PCR system's high-level performance lets you:
- Detect rare DNA target copies with unmatched sensitivity
- Measure small fold differences with the highest precision
The QX100 system consists of two components: the QX100 droplet generator and the QX100 droplet reader. A Bio-Rad thermal cycler equipped with a 96–deep well reaction module is also needed to complete the Droplet Digital PCR workflow.
The QX100 droplet generator partitions samples containing cDNA or RNA template into 20,000 nanoliter-sized droplets. After PCR, droplets from each sample are streamed in single file through the QX100 droplet reader. The PCR-positive and PCR-negative droplets are counted to provide absolute quantification of target DNA in digital form.
Watch this video describing how the QX100 Droplet Digital PCR system works.
There are many research areas that can benefit from digital PCR technology, including HIV research, cancer studies, and psychological disorders. The applications enabled by the ability of Droplet Digital PCR technology to achieve absolute quantification are described in numerous scientfic journal publications, conference poster presentations, our own BioRadiations articles, and Bio-Rad technical reports.
Watch our researcher testimonial video and read selected researcher profiles to discover how scientists in diverse fields apply digital PCR technology using the QX100 Droplet Digital PCR system. These researchers and others are using the QX100 system to help solve research problems in a variety of applications including, but not limited to, the following:
- Copy number variation (CNV)
- Rare sequence detection
- Mutation detection
- Gene expression analysis of rare transcripts
- miRNA analysis
- Next-generation sequencing sample quantification
View the complete QX100 system workflow.
The QX100 Droplet Digital PCR system requires the use of either the C1000 Touch™ or S1000™ thermal cycler with 96–deep well reaction module.
Bio-Rad offers exclusive accessories and PCR reagent kits for use with the ddPCR system. PCR reagent kits include 2x supermix kits with probe-based target detection and one-step RT-ddPCR kits (with probes). The PX1™ PCR plate sealer effectively seals PCR plates prior to thermal cycling. The pierceable foil heat seal provides a secure sealing option for digital PCR applications.
Explore the features of the QX100 system in more depth in the QX100™ Droplet Digital™ PCR System Brochure (bulletin 6237) or contact your Bio-Rad sales representative for more information.