Detergents and Blocking Reagents for Blotting

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Detergents and Blocking Reagents
1x PBS/1% casein 10 mM sodium phosphate, 150 mM NaCl, 1% (w/v) casein, pH 7.4 Western blotting wash solution (casein blockers recommended for all applications, including those with biotin-avidin complexes)
1x TBS/1% casein 20 mM Tris, 500 mM NaCl, 1% (w/v) casein, pH 7.4 Western blotting wash solution (casein blockers recommended for all applications, including those with biotin-avidin complexes)
10% Tween 20 10% (w/v) Tween 20 Blocking and wash buffer component




The processing of blots is now even simpler with a variety of premixed wash buffers and blocking solutions:

  • Premixed blocking buffers, available as PBS/casein and TBS/casein, take the time and effort out of solubilizing casein
  • 10% Tween 20 makes pipetting of Tween 20 accurate and simple
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Gelatin

1706537
200 g, enzyme immunoassay grade gelatin

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$56.00
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Blotting-Grade Blocker
Blotting-Grade Blocker

1706404
300 g, nonfat dry milk for western blotting applications

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$47.00
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Tween 20, 100% Nonionic Detergent
Tween 20, 100% Nonionic Detergent

1706531
100 ml, enzyme immunoassay grade polysorbate surfactant (detergent)

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$53.00
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10% Tween 20, Nonionic Detergent
10% Tween 20, Nonionic Detergent

1610781
1 L, detergent, for easy pipetting

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$36.00
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20% SDS Solution, 1 L

1610418
1 L, 20% (w/v) sodium dodecyl sulfate (SDS) solution

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$120.00
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1x Phosphate Buffered Saline (PBS) with 1% Casein
1x Phosphate Buffered Saline (PBS) with 1% Casein

1610783
1 L, blocking reagent

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$94.00
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1x Tris Buffered Saline (TBS) with 1% Casein
1x Tris Buffered Saline (TBS) with 1% Casein

1610782
1 L, blocking reagent

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$94.00
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Number Description Options
2317 Ready-to-Run Buffers and Solutions Brochure, Rev F Click to download