Detergents and Blocking Reagents for Blotting

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Detergents and Blocking Reagents for Blotting

Overview

Detergents and Blocking Reagents
1x PBS/1% casein 10 mM sodium phosphate, 150 mM NaCl, 1% (w/v) casein, pH 7.4 Western blotting wash solution (casein blockers recommended for all applications, including those with biotin-avidin complexes)
1x TBS/1% casein 20 mM Tris, 500 mM NaCl, 1% (w/v) casein, pH 7.4 Western blotting wash solution (casein blockers recommended for all applications, including those with biotin-avidin complexes)
10% Tween 20 10% (w/v) Tween 20 Blocking and wash buffer component
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The processing of blots is now even simpler with a variety of premixed wash buffers and blocking solutions:

  • Premixed blocking buffers, available as PBS/casein and TBS/casein, take the time and effort out of solubilizing casein
  • 10% Tween 20 makes pipetting of Tween 20 accurate and simple
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Gelatin

1706537
200 g, enzyme immunoassay grade gelatin

0 1706537 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6537_0921120402643.xml 1706537 09/21/09 12:04 PM N en 09/21/19 12:04 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:34 1706537-gelatin 1706537 Gelatin Gelatin 200 g, enzyme immunoassay grade gelatin 200 g, enzyme immunoassay grade gelatin 200 g, enzyme immunoassay grade gelatin /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-6537_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-6537_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-6537_feature.jpg RepEx 170-6537 200 g, enzyme immunoassay grade gelatin 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706537&CartQuote=QT&quantity= false true false $59.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706537&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706537&quantity= 1706537
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Blotting-Grade Blocker

1706404
300 g, nonfat dry milk for western blotting applications

1 1706404 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6404_0921120401756.xml 1706404 09/21/09 12:04 PM en_US true internet/product_SKU 2018-09-19 18:39:51 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6404_0921120401756.xml /default/main/bio-rad/WORKAREA/default ja9sm559 2017-11-14 07:08:34 true ja9sm559 templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6404_0921120401756.xml LSR N /default/main/bio-rad en 09/21/19 12:04 PM 1520368583752 2018-09-19 18:39:51 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6404_0921120401756.xml 1706404-blotting-grade-blocker 1 1706404 Blotting-Grade Blocker Blotting-Grade Blocker 300 g, nonfat dry milk for western blotting applications 300 g, nonfat dry milk for western blotting applications 300 g, nonfat dry milk for western blotting applications /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-6404_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-6404_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-6404_feature.jpg Blotting-Grade Blocker preview RepEx 170-6404 300 g, nonfat dry milk for western blotting applications 170-6404, 1706404, blotting-grade, blocker, nonfat, dry, milk, blotting, grade, western, blot, nonspecific, binding, immunodetection 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Use blotting-grade blocker for consistently low backgrounds on western blots.</p> <h2 class="descHeader">Related Products</h2> <p>If using an avidin-biotin detection system, use Tris- Buffered Saline with 1% Casein (<a href="/en-us/sku/1610782-1x-tris-buffered-saline-tbs-with-1-casein?ID=1610782">1610782</a>) or Phosphate- Buffered Saline with 1% Casein (<a href="/en-us/sku/1610783-1x-phosphate-buffered-saline-pbs-with-1-casein?ID=1610783">1610783</a>).</p> <ul> <li><a href="/en-us/product/detergents-blocking-reagents-for-blotting">Detergents and Blocking Reagents for Blotting </a></li> <li><a href="/en-us/category/western-blotting-membranes-filter-paper?ID=ef19d64e-5909-450d-9d71-cc5e59555033">Western Blotting Membranes and Filter Paper</a></li> <li><a href="/en-us/category/transfer-buffers">Transfer Buffers</a></li> <li><a href="/en-us/category/immunodetection-reagents-kits?ID=534c009d-cd86-4b33-956c-674d5a6ae054">Immunodetection Reagents and Kits</a> including <a href="/en-us/product/clarity-clarity-max-western-ecl-blotting-substrates?ID=e08909eb-3369-42ec-a585-df189004268f">Clarity&trade; ECL Western Blotting Substrate</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1706404.html /webroot/web/html/lsr/products/SKUs/autopopulated/1706404.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706404&CartQuote=QT&quantity= false true false $49.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706404&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706404&quantity= 1706404
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Tween 20, 100% Nonionic Detergent

1706531
100 ml, enzyme immunoassay grade polysorbate surfactant (detergent)

2 1706531 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6531_0921120402511.xml 1706531 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="production"> <Deployment Id="StandardMetadata.ja9sm5cyja9sm5d0" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="ja9sm5cyja9sm5d0"> <Name>StandardMetadata</Name> <TargetEnviroment>production</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="11" DayOfMonth="22" DayOfWeek="3" Year="2017" Hour="23" Minute="21" Second="6" ZoneOffsetMillis="0" Time="1511392866580">Wed Nov 22 23:21:06 UTC 2017</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6531_0921120402511.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="11" DayOfMonth="22" DayOfWeek="3" Year="2017" Hour="23" Minute="21" Second="6" ZoneOffsetMillis="0" Time="1511392866580">Wed Nov 22 23:21:06 UTC 2017</LastExecutionTime> </Node> <Node Name="QA"> <Deployment Id="StandardMetadata.jd0nat4ejd0nat4f" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jd0nat4ejd0nat4f"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="30" DayOfWeek="2" Year="2018" Hour="0" Minute="5" Second="26" ZoneOffsetMillis="0" Time="1517270726484">Tue Jan 30 00:05:26 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6531_0921120402511.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="30" DayOfWeek="2" Year="2018" Hour="0" Minute="5" Second="26" ZoneOffsetMillis="0" Time="1517270726484">Tue Jan 30 00:05:26 UTC 2018</LastExecutionTime> </Node> <Node Name="PROD"> <Deployment Id="StandardMetadata.jd0nau7xjd0nau7y" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jd0nau7xjd0nau7y"> <Name>StandardMetadata</Name> <TargetEnviroment>PROD</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="31" DayOfWeek="3" Year="2018" Hour="3" Minute="34" Second="33" ZoneOffsetMillis="0" Time="1517369673763">Wed Jan 31 03:34:33 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/170-6531_0921120402511.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="31" DayOfWeek="3" Year="2018" Hour="3" Minute="34" Second="33" ZoneOffsetMillis="0" Time="1517369673763">Wed Jan 31 03:34:33 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:04 PM en_US ja9sm56l 2017-11-14 07:08:34 true true internet/product_SKU LSR N en 09/21/19 12:04 PM 1517369755966 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-01 00:22:46 1706531-tween-20-100-nonionic-detergent 1706531 Tween 20 Tween 20, 100% Nonionic Detergent 100 ml, enzyme immunoassay grade polysorbate surfactant (detergent) 100 ml, enzyme immunoassay grade polysorbate surfactant (detergent) 100 ml, enzyme immunoassay grade polysorbate surfactant (detergent) /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-6531_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-6531_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-6531_feature.jpg Tween 20, 100% Nonionic Detergent preview RepEx 170-6531 100 ml, enzyme immunoassay grade polysorbate surfactant (detergent) 170-6531, 1706531, tween, 20, tween-20, enzyme, immunoassay, grade, polysorbate, surfactant, detergent, dna, rna, western, blot, blots, blotting, elisa, protein, sample, preparation, buffer 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Choose 100% Tween 20 for applications including protein extractions, and blocking and washing of western blots and ELISAs.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>10% (w/v) Tween 20, Nonionic Detergent (<a href="/en-us/sku/1610781-10-tween-20-nonionic-detergent?ID=1610781">1610781</a>)</li> <li><a href="/en-us/product/precisionab-validated-western-blotting-antibodies">PrecisionAb&trade; Validated Western Blotting Antibodies</a></li> <li>Criterion&trade; Staining/Blotting Trays with Lids (<a href="/en-us/sku/3459921-criterion-staining-blotting-trays">3459921</a>)</li> <li>10x Phosphate Buffered Saline (PBS) (<a href="/en-us/sku/1610780-10x-phosphate-buffered-saline-pbs?ID=1610780">1610780</a>)</li> <li>10x Tris Buffered Saline (TBS) (<a href="/en-us/sku/1706435-10x-tris-buffered-saline-tbs?ID=1706435-tbs">1706435</a>)</li> <li>1x Phosphate Buffered Saline (PBS) with 1% Casein (<a href="/en-us/sku/1610783-1x-phosphate-buffered-saline-pbs-with-1-casein">1610783</a>)</li> <li>1x Tris Buffered Saline (TBS) with 1% Casein (<a href="/en-us/sku/1610782-1x-tris-buffered-saline-tbs-with-1-casein?ID=1610782">1610782</a>)</li> <li>Blotting-Grade Blocker, nonfat dry milk (<a href="/en-us/sku/1706404-blotting-grade-blocker">1706404</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-gels">Protein Gels</a></li> <li><a href="http://www.bio-rad.com/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452">Western Blotting Membranes and Filter Paper</a></li> <li><a href="/en-us/category/semi-dry-rapid-blotting-systems">Semi-Dry and Rapid Blotting Systems</a></li> <li><a href="/en-us/category/wet-tank-blotting-systems">Wet/Tank Blotting Systems</a></li> <li><a href="/en-us/category/power-supplies?ID=5f7dc809-5983-461f-90c9-4610c7d6f780">Power Supplies</a></li> <li><a href="/en-us/category/transfer-buffers">Transfer Buffers</a></li> <li><a href="/en-us/category/immunodetection-reagents-kits?ID=534c009d-cd86-4b33-956c-674d5a6ae054">Immunodetection Reagents and Kits</a></li> <li><a href="/en-us/category/gel-imaging-systems?ID=d6f93330-755b-410c-9c9c-77da24d51eab">Gel and Western Imaging Systems</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Mini-PROTEAN<sup>&reg;</sup> Precast Gels <a href="/webroot/web/pdf/lsr/literature/Bulletin_1658100.pdf" target="_blank">Instruction Manual and Application Guide</a></li> <li>Criterion Gel Application Guide <a href="/webroot/web/pdf/lsr/literature/4110001.pdf" target="_blank">Instruction Manual</a></li> <li>Mini Trans-Blot<sup>&reg;</sup> Cell <a href="/webroot/web/pdf/lsr/literature/M1703930.pdf" target="_blank">Instruction Manual</a></li> <li>Trans-Blot SD Cell <a href="/webroot/web/pdf/lsr/literature/4006066B.pdf">Quick Reference Guide</a></li> <li>Trans-Blot SD Semi-Dry Transfer Cell <a href="/webroot/web/pdf/lsr/literature/M1703940.pdf" target="_blank">Instruction Manual</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B">Protein Electrophoresis</a></li> <li><a href="/en-us/applications-technologies/introduction-western-blotting?ID=LUSPPAKG4">Western Blotting</a></li> <li><a href="/en-us/applications-technologies/imaging-analysis">Imaging and Analysis</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1706531.html /webroot/web/html/lsr/products/SKUs/autopopulated/1706531.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706531&CartQuote=QT&quantity= false true false $56.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706531&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706531&quantity= 1706531
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10% Tween 20, Nonionic Detergent

1610781
1 L, detergent, for easy pipetting

3 1610781 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0781_0921120402578.xml 1610781 09/21/09 12:04 PM N en 09/21/19 12:04 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2018-02-01 00:22:44 1610781-10-tween-20-nonionic-detergent 1610781 10% Tween 20 10% Tween 20, Nonionic Detergent 1 L, detergent, for easy pipetting 1 L, detergent, for easy pipetting 1 L, detergent, for easy pipetting /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0781_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0781_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0781_feature.jpg 10% Tween 20, Nonionic Detergent preview RepEx 161-0781 1 L, detergent, for easy pipetting 161-0781, 1610781, 10%, tween, 20, tween-20, easy, pipetting, immunoassay, polysorbate, surfactant, detergent, dna, rna, western, blot, blots, blotting, elisa, protein, sample, preparation, buffer 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Choose 10% (w/v) Tween 20 for accurate pipetting of Tween 20 for applications such as protein extractions, and blocking and washing of western blots and ELISAs.</p> <h2 class="descHeader">Related Products</h2> <ul> <li>Tween 20, 100% Nonionic Detergent (<a href="/en-us/sku/1706531-tween-20-100-nonionic-detergent?ID=1706531">1706531</a>)</li> <li><a href="/en-us/product/precisionab-validated-western-blotting-antibodies">PrecisionAb&trade; Validated Western Blotting Antibodies</a></li> <li>Criterion&trade; Staining/Blotting Trays with Lids (<a href="/en-us/sku/3459921-criterion-staining-blotting-trays">3459921</a>)</li> <li>10x Phosphate Buffered Saline (PBS) (<a href="/en-us/sku/1610780-10x-phosphate-buffered-saline-pbs?ID=1610780">1610780</a>)</li> <li>10x Tris Buffered Saline (TBS) (<a href="/en-us/sku/1706435-10x-tris-buffered-saline-tbs?ID=1706435">1706435</a>)</li> <li>1x Phosphate Buffered Saline (PBS) with 1% Casein (<a href="/en-us/sku/1610783-1x-phosphate-buffered-saline-pbs-with-1-casein?ID=1610783">1610783</a>)</li> <li>1x Tris Buffered Saline (TBS) with 1% Casein (<a href="/en-us/sku/1610782-1x-tris-buffered-saline-tbs-with-1-casein?ID=1610782">1610782</a>)</li> <li>Blotting-Grade Blocker, nonfat dry milk (<a href="/en-us/sku/1706404-blotting-grade-blocker">1706404</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-gels">Protein Gels</a></li> <li><a href="http://www.bio-rad.com/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452">Western Blotting Membranes and Filter Paper</a></li> <li><a href="/en-us/category/semi-dry-rapid-blotting-systems">Semi-Dry and Rapid Blotting Systems</a></li> <li><a href="/en-us/category/wet-tank-blotting-systems">Wet/Tank Blotting Systems</a></li> <li><a href="/en-us/category/power-supplies?ID=5f7dc809-5983-461f-90c9-4610c7d6f780">Power Supplies</a></li> <li><a href="/en-us/category/transfer-buffers">Transfer Buffers</a></li> <li><a href="/en-us/category/immunodetection-reagents-kits?ID=534c009d-cd86-4b33-956c-674d5a6ae054">Immunodetection Reagents and Kits</a></li> <li><a href="/en-us/category/gel-imaging-systems?ID=d6f93330-755b-410c-9c9c-77da24d51eab">Gel and Western Imaging Systems</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Mini-PROTEAN<sup>&reg;</sup> Precast Gels <a href="/webroot/web/pdf/lsr/literature/Bulletin_1658100.pdf" target="_blank">Instruction Manual and Application Guide</a></li> <li>Criterion Gel Application Guide <a href="/webroot/web/pdf/lsr/literature/4110001.pdf" target="_blank">Instruction Manual</a></li> <li>Mini Trans-Blot<sup>&reg;</sup> Cell <a href="/webroot/web/pdf/lsr/literature/M1703930.pdf" target="_blank">Instruction Manual</a></li> <li>Trans-Blot SD Cell <a href="/webroot/web/pdf/lsr/literature/4006066B.pdf">Quick Reference Guide</a></li> <li>Trans-Blot SD Semi-Dry Transfer Cell <a href="/webroot/web/pdf/lsr/literature/M1703940.pdf" target="_blank">Instruction Manual</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B">Protein Electrophoresis</a></li> <li><a href="/en-us/applications-technologies/introduction-western-blotting?ID=LUSPPAKG4">Western Blotting</a></li> <li><a href="/en-us/applications-technologies/imaging-analysis">Imaging and Analysis</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1610781.html /webroot/web/html/lsr/products/SKUs/autopopulated/1610781.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610781&CartQuote=QT&quantity= false true false $38.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610781&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610781&quantity= 1610781
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20% SDS Solution, 1 L

1610418
1 L, 20% (w/v) sodium dodecyl sulfate (SDS) solution

4 1610418 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0418_0921120402530.xml 1610418 09/21/09 12:04 PM N en 09/21/19 12:04 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2018-02-01 00:22:43 1610418-20-sds-solution-1-l 1610418 SDS Solution 20% SDS Solution, 1 L 1 L, 20% (w/v) sodium dodecyl sulfate (SDS) solution 1 L, 20% (w/v) sodium dodecyl sulfate (SDS) solution 1 L, 20% (w/v) sodium dodecyl sulfate (SDS) solution /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0418_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0418_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0418_feature.jpg preview RepEx 161-0418 1 L, 20% (w/v) sodium dodecyl sulfate (SDS) solution 1610418, 1610416, sds, solution, 10%, 20%, detergent, sodium, dodecyl, sulfate, gel, electrophoresis, blotting, detergents, premixed, buffer 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Use 20% SDS (sodium dodecyl sulfate) solution for any applications requiring SDS such as PAGE, nucleic acid hybridization, and cell disruption. The SDS solution is highly purified and provides reproducible protein mobility in electrophoresis.</p> <ul> <li>High purity</li> <li>No detectable DNase or RNase activity</li> <li>Prepared with 18 MΩ water</li> </ul> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="319" valign="top"><strong>SDS Percentage (w/v)<br /></strong></td> <td><strong>Volume</strong></td> <td style="text-align: left;" width="319" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td valign="top">10%</td> <td>250 ml</td> <td style="text-align: left;" valign="top"><a href="/en-us/sku/1610416-10-sds-solution-250-ml?ID=1610416">1610416</a></td> </tr> <tr> <td valign="top">20%</td> <td>1 L</td> <td style="text-align: left;" valign="top">1610418</td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/acrylamide-solutions-powders?ID=5e7a4f31-879c-4d63-ba0b-82556a0ccf1d">Acrylamide Solutions and Powders</a></li> <li>Glycine (<a href="/en-us/sku/1610718-glycine-1-kg?ID=1610718">1610718</a>)</li> <li>Tris (<a href="/en-us/sku/1610719-tris">1610719</a>)</li> <li>TEMED (<a href="/en-us/sku/1610800-temed">1610800</a>)</li> <li>Ammonium Persulfate (APS) (<a href="/en-us/sku/1610700-ammonium-persulfate-aps">1610700</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-gels">Protein Gels</a></li> <li><a href="/en-us/category/polyacrylamide-gel-reagents?ID=ce5bc208-a990-4043-ad17-e7bd874bf301">Polyacrylamide Gel Reagents</a></li> <li><a href="/en-us/category/protein-ladders-standards-markers">Protein Ladders and Standards (Markers)</a></li> <li><a href="/en-us/category/electrophoresis-chambers">Electrophoresis Chambers</a></li> <li><a href="/en-us/category/nucleic-acid-blotting">Nucleic Acid Blotting</a></li> <li><a href="/en-us/category/power-supplies?ID=5f7dc809-5983-461f-90c9-4610c7d6f780">Power Supplies</a></li> <li><a href="/en-us/category/protein-extraction">Protein Extraction</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B">Protein Electrophoresis</a></li> <li><a href="/en-us/applications-technologies/protein-purification-isolation">Protein Purification and Isolation</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1610418.html /webroot/web/html/lsr/products/SKUs/autopopulated/161-0418.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610418&CartQuote=QT&quantity= false true false $129.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610418&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610418&quantity= 1610418
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1x Phosphate Buffered Saline (PBS) with 1% Casein

1610783
1 L, blocking reagent

5 1610783 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0783_0921120402622.xml 1610783 09/21/09 12:04 PM N en 09/21/19 12:04 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2018-02-01 00:22:44 1610783-1x-phosphate-buffered-saline-pbs-with-1-casein 1610783 1x Phosphate Buffered Saline With 1% Casein 1x Phosphate Buffered Saline (PBS) with 1% Casein 1 L, blocking reagent 1 L, blocking reagent, 10 mM sodium phosphate, 150 mM NaCl, 1% (w/v) casein, pH 7.4 1 L, blocking reagent /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0783_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0783_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0783_feature.jpg 1x Phosphate Buffered Saline (PBS) with 1% Casein RepEx 161-0783 1 L, blocking reagent, 10 mM sodium phosphate, 150 mM NaCl, 1% (w/v) casein, pH 7.4 161-0783, 1610783, 1x, phosphate, buffered, saline, 1%, casein, pbs, western, blot, blots, blotting, buffer, buffers 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Use Phosphate Buffered Saline (PBS) with 1% (w/v) Casein for blocking membranes for blotting. Blocking buffers containing casein may provide lower backgrounds than buffers containing low fat milk, and casein is recommended for applications using biotin-avidin complexes. When using alkaline phosphatase as a reporter in western blots, it is recommended that Phosphate Buffered Saline (PBS) be replaced with Tris Buffered Saline (TBS).</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/precisionab-validated-western-blotting-antibodies">PrecisionAb&trade; Validated Western Blotting Antibodies</a></li> <li>Criterion&trade; Staining/Blotting Trays with Lids (<a href="/en-us/sku/3459921-criterion-staining-blotting-trays">3459921</a>)</li> <li>10x Tris Buffered Saline (TBS) (<a href="/en-us/sku/1706435-10x-tris-buffered-saline-tbs?ID=1706435-tbs">1706435</a>)</li> <li>10x Phosphate Buffered Saline (PBS) (<a href="/en-us/sku/1610780-10x-phosphate-buffered-saline-pbs?ID=1610780">1610780</a>)</li> <li>1x Tris Buffered Saline (TBS) with 1% Casein (<a href="/en-us/sku/1610782-1x-tris-buffered-saline-tbs-with-1-casein?ID=1610782">1610782</a>) </li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-gels">Protein Gels</a></li> <li><a href="http://www.bio-rad.com/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452">Western Blotting Membranes and Filter Paper</a></li> <li><a href="/en-us/category/semi-dry-rapid-blotting-systems">Semi-Dry and Rapid Blotting Systems</a></li> <li><a href="/en-us/category/wet-tank-blotting-systems">Wet/Tank Blotting Systems</a></li> <li><a href="/en-us/category/power-supplies?ID=5f7dc809-5983-461f-90c9-4610c7d6f780">Power Supplies</a></li> <li><a href="/en-us/category/transfer-buffers">Transfer Buffers</a></li> <li><a href="/en-us/category/immunodetection-reagents-kits?ID=534c009d-cd86-4b33-956c-674d5a6ae054">Immunodetection Reagents and Kits</a></li> <li><a href="/en-us/category/gel-imaging-systems?ID=d6f93330-755b-410c-9c9c-77da24d51eab">Gel and Western Imaging Systems</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Mini-PROTEAN<sup>&reg;</sup> Precast Gels <a href="/webroot/web/pdf/lsr/literature/Bulletin_1658100.pdf" target="_blank">Instruction Manual and Application Guide</a></li> <li>Criterion Gel Application Guide <a href="/webroot/web/pdf/lsr/literature/4110001.pdf" target="_blank">Instruction Manual</a></li> <li>Mini Trans-Blot<sup>&reg;</sup> Cell <a href="/webroot/web/pdf/lsr/literature/M1703930.pdf" target="_blank">Instruction Manual</a></li> <li>Trans-Blot SD Cell <a href="/webroot/web/pdf/lsr/literature/4006066B.pdf">Quick Reference Guide</a></li> <li>Trans-Blot SD Semi-Dry Transfer Cell <a href="/webroot/web/pdf/lsr/literature/M1703940.pdf" target="_blank">Instruction Manual</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B">Protein Electrophoresis</a></li> <li><a href="/en-us/applications-technologies/introduction-western-blotting?ID=LUSPPAKG4">Western Blotting</a></li> <li><a href="/en-us/applications-technologies/imaging-analysis">Imaging and Analysis</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1610783.html /webroot/web/html/lsr/products/SKUs/autopopulated/1610783.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610783&CartQuote=QT&quantity= false true false $99.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610783&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610783&quantity= 1610783
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Communication error

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1x Tris Buffered Saline (TBS) with 1% Casein

1610782
1 L, blocking reagent

6 1610782 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0782_0921120402600.xml 1610782 09/21/09 12:04 PM N en 09/21/19 12:04 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2018-02-01 00:22:44 1610782-1x-tris-buffered-saline-tbs-with-1-casein 1610782 1x Tris Buffered Saline With 1% Casein 1x Tris Buffered Saline (TBS) with 1% Casein 1 L, blocking reagent 1 L, detergent, 20mM Tris, 500 mM NaCl, containing 1% (w/v) casein, pH 7.4 1 L, blocking reagent /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0782_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0782_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0782_feature.jpg 1x Tris Buffered Saline (TBS) with 1% Casein RepEx 161-0782 1 L, detergent, 20mM Tris, 500 mM NaCl, containing 1% (w/v) casein, pH 7.4 161-0782, 1610782, 1x, tris, buffered, saline, 1%, casein, tbs, blocking, blocker, reagent, wash, buffer, blot, blots, western, blotting, westerns 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Use Tris Buffered Saline (TBS) with 1% (w/v) Casein for blocking membranes for blotting. Blocking buffers containing casein can provide lower backgrounds than buffers containing low fat milk, and casein is recommended for applications using biotin-avidin complexes. Tris Buffered Saline (TBS) with 1% (w/v) Casein is recommended for applications using alkaline phosphate since unlike phosphate-containing buffers it does not interfere with enzyme activity.</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/precisionab-validated-western-blotting-antibodies">PrecisionAb&trade; Validated Western Blotting Antibodies</a></li> <li>Criterion&trade; Staining/Blotting Trays with Lids (<a href="/en-us/sku/3459921-criterion-staining-blotting-trays">3459921</a>)</li> <li>10x Tris Buffered Saline (TBS) (<a href="/en-us/sku/1706435-10x-tris-buffered-saline-tbs?ID=1706435">1706435</a>)</li> <li>10x Phosphate Buffered Saline (PBS) (<a href="/en-us/sku/1610780-10x-phosphate-buffered-saline-pbs?ID=1610780">1610780</a>)</li> <li>1x Phosphate Buffered Saline (PBS) with 1% Casein (<a href="/en-us/sku/1610783-1x-phosphate-buffered-saline-pbs-with-1-casein?ID=1610783">1610783</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-gels">Protein Gels</a></li> <li><a href="http://www.bio-rad.com/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452">Western Blotting Membranes and Filter Paper</a></li> <li><a href="/en-us/category/semi-dry-rapid-blotting-systems">Semi-Dry and Rapid Blotting Systems</a></li> <li><a href="/en-us/category/wet-tank-blotting-systems">Wet/Tank Blotting Systems</a></li> <li><a href="/en-us/category/power-supplies?ID=5f7dc809-5983-461f-90c9-4610c7d6f780">Power Supplies</a></li> <li><a href="/en-us/category/transfer-buffers">Transfer Buffers</a></li> <li><a href="/en-us/category/immunodetection-reagents-kits?ID=534c009d-cd86-4b33-956c-674d5a6ae054">Immunodetection Reagents and Kits</a></li> <li><a href="/en-us/category/gel-imaging-systems?ID=d6f93330-755b-410c-9c9c-77da24d51eab">Gel and Western Imaging Systems</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Mini-PROTEAN<sup>&reg;</sup> Precast Gels <a href="/webroot/web/pdf/lsr/literature/Bulletin_1658100.pdf" target="_blank">Instruction Manual and Application Guide</a></li> <li>Criterion Gel Application Guide <a href="/webroot/web/pdf/lsr/literature/4110001.pdf" target="_blank">Instruction Manual</a></li> <li>Mini Trans-Blot<sup>&reg;</sup> Cell <a href="/webroot/web/pdf/lsr/literature/M1703930.pdf" target="_blank">Instruction Manual</a></li> <li>Trans-Blot SD Cell <a href="/webroot/web/pdf/lsr/literature/4006066B.pdf">Quick Reference Guide</a></li> <li>Trans-Blot SD Semi-Dry Transfer Cell <a href="/webroot/web/pdf/lsr/literature/M1703940.pdf" target="_blank">Instruction Manual</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B">Protein Electrophoresis</a></li> <li><a href="/en-us/applications-technologies/introduction-western-blotting?ID=LUSPPAKG4">Western Blotting</a></li> <li><a href="/en-us/applications-technologies/imaging-analysis">Imaging and Analysis</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1610782.html /webroot/web/html/lsr/products/SKUs/autopopulated/1610782.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610782&CartQuote=QT&quantity= false true false $99.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610782&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610782&quantity= 1610782
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2317 Ready-to-Run Buffers and Solutions Brochure, Rev F Click to download

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