ddPCR™ Supermix for Probes

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This digital PCR supermix for probes is a 2x concentrated, ready-to-use universal mix that has been optimized to deliver maximum PCR efficiency, specificity, and sensitivity in Droplet Digital™ PCR (ddPCR™).

This supermix is suitable for use with UNG decontamination protocols.

Key Benefits

  • Contains all components required for hydrolysis probe–based ddPCR except primers, probe(s), and template
  • Limits nonspecific PCR amplification
  • Hot-start polymerase keeps enzyme inactive during sample droplet partitioning

Applications and Uses

  • Absolute quantification
  • Mutation detection
  • Copy number analysis
  • Gene expression analysis

Packaging Options

Number of 20 µl Reactions Supermix Volume, ml Catalog Number
200 2 x 1 186-3026
500 5 x 1 186-3010
2,500 5 x 5 186-3027
5,000 10 x 5 186-3028

Additional Reagents

Bio-Rad offers additional consumables for completing your digital PCR workflow:

Additional Equipment

These digital PCR assays require the use of the following instruments and accessories:

Related Products

Bio-Rad offers additional digital PCR supermixes including:

More Information

The ddPCR Supermix for Probes (no dUTP) is optimized for use with Droplet Generation Oil for Probes on the QX200™ Droplet Digital™ PCR System.

Storage at –20°C Up to 18 months (refer to expiration date)
Storage at 4°C (after thawing) Up to 2 weeks
Template compatibility cDNA, genomic DNA, plasmid DNA
x

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ddPCR™ Supermix for Probes

1863026
2 ml (2 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems

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$170.00
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ddPCR™ Supermix for Probes

1863010
5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems

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$425.00
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ddPCR™ Supermix for Probes

1863027
25 ml (5 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems

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$2,115.00
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ddPCR™ Supermix for Probes

1863028
50 ml (10 x 5 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200™/QX100™ Droplet Digital™ PCR Systems

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$4,225.00
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Number Description Options
6338 Droplet Digital™ PCR (ddPCR™) Supermixes Flier, Rev A Click to download [ Add to Cart (Free) ]
10026235 ddPCR™ Supermix for Probes Product Insert, Rev C Click to download
10040718 Droplet Generation Oil for Probes Product Insert, Rev B Click to download
6311 QX200™ Droplet Digital™ PCR System Brochure, Rev C Click to download [ Add to Cart (Free) ]
6450 Droplet Digital™ PCR Publications List, Rev K Click to download
10031907 Instruction Manual, QX200™ Droplet Generator, Rev C Click to download
10031906 Instruction Manual, QX200™ Droplet Reader and QuantaSoft™ Software, Rev D Click to download