DC™ Protein Assay

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<em>DC</em> Protein Assay

Overview

Specifications
Protein Concentration Test Tube Microplate
High-concentration assay 0.2–1.5 mg/ml protein 0.2–1.5 mg/ml protein
  100 µl sample 5 µl sample
  500 µl reagent A 25 µl reagent A
  4.0 ml reagent B 200 µl reagent B
Low-concentration assay 5–250 µg/ml protein 5–250 µg/ml protein
  200 µl sample 20 µl sample
  100 µl reagent A 10 µl reagent A
  800 µl reagent B 80 µl reagent B
Compatible Reagents*
Brij 35, 1% Guanidine-HCl, 0.4 M Sodium hydroxide, 0.5 M
C12E8,** 0.2% HCl, 0.5 M Thesit, 1%
Calcium chloride, 50 mM (NH4)2SO4, 0.5 M Tris, pH 8, 0.1 M
CHAPS, 1% Nonidet P-40 (NP-40), 2% Triton X-100, 1%
CHAPSO 1% Octyl-β-glucoside, 1% Tween 20, 1%
DTT, 1 mM SDS, 10% Urea 4 M
EDTA, <1 mM Sodium azide, 0.05%  
* Using standard procedure.
** Octaethyleneglycol dodecyl ether.
Protein Assay Selection Guide

Chart RC DC Protein Assay DC Protein Assay Quick Start Bradford Assay Assay Bio-Rad Protein Assay

  * Nonidet P-40 >0.25%, Triton C-100 >0.05%, Tween 20 >0.01%, SDS >0.025%
** DTT >1 mM, EDTA >1 mM

Protein Assay Comparison
Characteristics of Bio-Rad's Protein Assays
  Protein Assay
Quick Start™ Bradford Bio-Rad DC RC DC™
Adapted from method of Bradford (1976) Bradford (1976) Lowry et al. (1951) Lowry et al. (1951)
Standard concentration assay
Sample volume 100 µl 100 µl 100 µl 100 µl
Linear range 0.125–1.5 mg/ml 0.2–1.5 mg/ml 0.125–1.5 mg/ml 0.125–1.5 mg/ml
Low-concentration assay
Sample volume 1 ml 800 µl 200 µl 200 µl
Linear range 1.25–25 µg/ml 1.25–25 µg/ml 5–250 µg/ml 5–250 µg/ml
Microplate assay sample volume 5 µl 10 µl 5 µl *
Minimum incubation time 5 min 5 min 15 min 15 min
Assay wavelength 595 nm 595 nm 650–750 nm 650–750 nm

All assays are easy to use, require little reagent preparation, and give accurate and reproducible results.

* To adapt the RC DC assay to a microplate format, follow the micro test tube (microfuge tube) assay protocol in the RC DC instruction manual up to the centrifugation step where the supernatant is discarded. The pellet can then be transferred to the microplate, and the microplate assay protocol in the DC protein assay manual can be followed.

In a typical protein assay, a chemical reagent is added to a protein sample, producing a visible result, such as a color change in the sample solution. This color change is quantitated with a spectrophotometer or microplate reader, and compared to a standard curve of known concentrations of protein versus their absorbance after reaction with the reagent. The amount of protein in the unknown sample is determined by interpolation, reading the concentration of protein on the standard curve that corresponds to its absorbance.

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The DC™ (detergent compatible) protein assay is a colorimetric assay for protein concentration following detergent solubilization. The reaction is similar to the well-documented Lowry assay, but has been modified to save time. The DC protein assay requires only a single 15 minute incubation, and absorbance is stable for at least 2 hours.

Using standard procedure, the assay is used with samples having protein concentrations between 0.2 and 1.5 mg/ml and requires just 100 µl of sample. The procedure can easily be adapted to a low-concentration microassay (see below). The microplate assay reduces the volume of sample required to just 5 µl. The DC protein assay is measured at 650–750 nm with a standard laboratory spectrophotometer or microplate reader.

DC protein assay kits offer either bovine serum albumin or bovine γ-globulin standard sets.

Microassay Capability
For lower concentrations of protein, the microassay procedure can be used in a standard test tube or in a microplate format. The microassay procedure can be used to measure protein concentrations from 5 to 250 µg/ml; it requires 200 µl of sample for the test tube assay or 20 µl of sample for the microplate assay. When using the microassay format, the kit contains enough reagent for 2,500 standard assays or 25,000 microplate assays.

Because the ratio of sample to reagent is changed, compatibility changes when using the microassay. The DC protein microassay is not compatible with 2-mercaptoethanol, dithiothreitol, or guanidine HCl.

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DC™ Protein Assay Kit I

5000111
Detergent-compatible colorimetric assay kit, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution, bovine γ-globulin standard; 450 assays

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Protein Assay Kit I Detergent-compatible colorimetric assay kit, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution, bovine &#947;-globulin standard; 450 assays Detergent-compatible colorimetric assay kit, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution, bovine &#947;-globulin standard; 450 assays Detergent-compatible colorimetric assay kit, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution, bovine &#947;-globulin standard; 450 assays /webroot/web/images/lsr/products/microplate_systems/sku_thumb/global/500-0111_tn.jpg /webroot/web/images/lsr/products/microplate_systems/sku_view/global/500-0111_view.jpg /webroot/web/images/lsr/products/microplate_systems/sku_feature/global/500-0111_feature.jpg preview RepEx 500-0111 Detergent-compatible colorimetric assay kit, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution, bovine &#947;-globulin standard; 450 assays 500-0111, 5000111, dc, protein, assay, detergent-compatible, detergent, compatible, sample, quantitation, concentration, colorimetric 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Sample-Quantitation/Protein-Assay-Kits-and-Cuvette N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000111&CartQuote=QT&quantity= true true false $201.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000111&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000111&quantity= 5000111
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DC™ Protein Assay Kit II

5000112
Detergent-compatible colorimetric assay kit, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution, BSA standard; 450 assays

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Protein Assay Kit II Detergent-compatible colorimetric assay kit, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution, BSA standard; 450 assays Detergent-compatible colorimetric assay kit, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution, BSA standard; 450 assays Detergent-compatible colorimetric assay kit, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution, BSA standard; 450 assays /webroot/web/images/lsr/products/microplate_systems/sku_thumb/global/500-0112_tn.jpg /webroot/web/images/lsr/products/microplate_systems/sku_view/global/500-0112_view.jpg /webroot/web/images/lsr/products/microplate_systems/sku_feature/global/500-0112_feature.jpg preview RepEx 500-0112 Detergent-compatible colorimetric assay kit, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution, BSA standard; 450 assays 500-0112, 5000112, dc, protein, assay, ii, detergent-compatible, detergent, compatible, sample, quantitation, concentration, colorimetric 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Sample-Quantitation/Protein-Assay-Kits-and-Cuvette N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000112&CartQuote=QT&quantity= true true false $185.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000112&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000112&quantity= 5000112
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Protein Standard I

5000005
Pkg of 1 vial, lyophilized bovine γ-globulin; 20 ml (reconstituted)

6 5000005 /templatedata/internet/product_SKU/data/LSR/Sample-Quantitation/Protein-Assay-Kits-and-Cuvette/500-0005_0921120600146.xml 5000005 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:09:00 5000005-protein-standard-i 5000005 Protein Standard I Protein Standard I Pkg of 1 vial, lyophilized bovine &#947;-globulin; 20 ml (reconstituted) Pkg of 1 vial, lyophilized bovine &#947;-globulin; 20 ml (reconstituted) Pkg of 1 vial, lyophilized bovine &#947;-globulin; 20 ml (reconstituted) /webroot/web/images/lsr/products/sample_quantitation/sku_thumb/global/500-0005_tn.jpg /webroot/web/images/lsr/products/sample_quantitation/sku_view/global/500-0005_view.jpg /webroot/web/images/lsr/products/sample_quantitation/sku_feature/global/500-0005_feature.jpg RepEx 500-0005 Pkg of 1 vial, lyophilized bovine &#947;-globulin; 20 ml (reconstituted) 500-0005, 5000005, protein, standard, bovine, gamma, globulin, gamma-globulin, dc, colorimetric, concentration, assay 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Sample-Quantitation/Protein-Assay-Kits-and-Cuvette N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000005&CartQuote=QT&quantity= false true false $78.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000005&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000005&quantity= 5000005
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Protein Standard II

5000007
Pkg of 1 vial, lyophilized bovine serum albumin, 20 ml (reconstituted)

7 5000007 /templatedata/internet/product_SKU/data/LSR/Sample-Quantitation/Protein-Assay-Kits-and-Cuvette/500-0007_0921120600108.xml 5000007 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:09:00 5000007-protein-standard-ii 5000007 Protein Standard II Protein Standard II Pkg of 1 vial, lyophilized bovine serum albumin, 20 ml (reconstituted) Pkg of 1 vial, lyophilized bovine serum albumin, 20 ml (reconstituted) Pkg of 1 vial, lyophilized bovine serum albumin, 20 ml (reconstituted) /webroot/web/images/lsr/products/sample_quantitation/sku_thumb/global/500-0007_tn.jpg /webroot/web/images/lsr/products/sample_quantitation/sku_view/global/500-0007_view.jpg /webroot/web/images/lsr/products/sample_quantitation/sku_feature/global/500-0007_feature.jpg RepEx 500-0007 Pkg of 1 vial, lyophilized bovine serum albumin, 20 ml (reconstituted) 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Sample-Quantitation/Protein-Assay-Kits-and-Cuvette N 0 500-0007, 5000007, protein, standard, ii, lyophilized, bovine, serum, albumin, dc, colorimetric, concentration, assay false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000007&CartQuote=QT&quantity= false true false $78.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000007&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000007&quantity= 5000007
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Semimicrovolume Disposable Polystyrene Cuvettes

2239955
Pkg of 100, 1.5 ml cuvette

8 2239955 /templatedata/internet/product_SKU/data/LSR/Liquid-Handling---Pipetting/Disposable-Cuvettes/223-9955_0921120510921.xml 2239955 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jcjsxz6mjcjsxz6n" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jcjsxz6mjcjsxz6n"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="19" DayOfWeek="5" Year="2018" Hour="20" Minute="55" Second="47" ZoneOffsetMillis="0" Time="1516395347205">Fri Jan 19 20:55:47 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Liquid-Handling---Pipetting/Disposable-Cuvettes/223-9955_0921120510921.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="19" DayOfWeek="5" Year="2018" Hour="20" Minute="55" Second="47" ZoneOffsetMillis="0" Time="1516395347205">Fri Jan 19 20:55:47 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:05 PM en_US jcjsxy7j 2017-11-14 07:08:52 true true internet/product_SKU LSR N en 09/21/19 12:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:08:52 2239955-semimicrovolume-disposable-polystyrene-cuvettes 2239955 Semimicrovolume Disposable Polystyrene Cuvettes Semimicrovolume Disposable Polystyrene Cuvettes Pkg of 100, 1.5 ml cuvette Pkg of 100, 1.5 ml cuvette Pkg of 100, 1.5 ml cuvette /webroot/web/images/lsr/products/sample_quantitation/sku_thumb/global/223-9955_tn.jpg /webroot/web/images/lsr/products/sample_quantitation/sku_view/global/223-9955_view.jpg /webroot/web/images/lsr/products/sample_quantitation/sku_feature/global/223-9955_feature.jpg preview RepEx 223-9955 Pkg of 100, 1.5 ml cuvette 223-9955, 2239955, semimicrovolume, disposable, polystyrene, cuvettes, semi-microvolume, semi, micro, volume, sample, quantitation, protein, assay, spectrophotmeter 09/21/09 12:05 PM 09/21/19 12:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Liquid-Handling---Pipetting/Disposable-Cuvettes N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=2239955&CartQuote=QT&quantity= false true false $12.88 true %2412.88 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=2239955&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=2239955&quantity= 2239955
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Standard Disposable Polystyrene Cuvettes

2239950
Pkg of 100, 3.5 ml cuvette

9 2239950 /templatedata/internet/product_SKU/data/LSR/Liquid-Handling---Pipetting/Disposable-Cuvettes/223-9950_0921120510941.xml 2239950 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jcjsxz6mjcjsxz6n" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jcjsxz6mjcjsxz6n"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="19" DayOfWeek="5" Year="2018" Hour="20" Minute="55" Second="47" ZoneOffsetMillis="0" Time="1516395347205">Fri Jan 19 20:55:47 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Liquid-Handling---Pipetting/Disposable-Cuvettes/223-9950_0921120510941.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="19" DayOfWeek="5" Year="2018" Hour="20" Minute="55" Second="47" ZoneOffsetMillis="0" Time="1516395347205">Fri Jan 19 20:55:47 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:05 PM en_US jcjsxy7h 2017-11-14 07:08:52 true true internet/product_SKU LSR N en 09/21/19 12:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:08:52 2239950-standard-disposable-polystyrene-cuvettes 2239950 Standard Disposable Polystyrene Cuvettes Standard Disposable Polystyrene Cuvettes Pkg of 100, 3.5 ml cuvette Pkg of 100, 3.5 ml cuvette Pkg of 100, 3.5 ml cuvette /webroot/web/images/lsr/products/sample_quantitation/sku_thumb/global/223-9950_tn.jpg /webroot/web/images/lsr/products/sample_quantitation/sku_view/global/223-9950_view.jpg /webroot/web/images/lsr/products/sample_quantitation/sku_feature/global/223-9950_feature.jpg preview RepEx 223-9950 Pkg of 100, 3.5 ml cuvette 223-9950, 2239950, standard, disposable, polystyrene, cuvettes, sample, quantitation, visible, range, 800–340, nm, semi-micro, protein, assay, bradford, cuvette, lowry 09/21/09 12:05 PM 09/21/19 12:05 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Liquid-Handling---Pipetting/Disposable-Cuvettes N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=2239950&CartQuote=QT&quantity= false true false $12.88 true %2412.88 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=2239950&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=2239950&quantity= 2239950
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Protein Assay Reagent A

5000113
250 ml, alkaline copper tartrate solution for colorimetric assays; replacement reagent for DC protein assay kits and RC DC protein assay kits

2 5000113 /templatedata/internet/product_SKU/data/LSR/Sample-Quantitation/Protein-Assay-Kits-and-Cuvette/500-0113_0921120600063.xml 5000113 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:09:00 5000113-protein-assay-reagent 5000113 Protein Assay Reagent A Protein Assay Reagent A 250 ml, alkaline copper tartrate solution for colorimetric assays; replacement reagent for <em>DC</em> protein assay kits and <em>RC DC</em> protein assay kits 250 ml, alkaline copper tartrate solution for colorimetric assays; replacement reagent for <em>DC</em> protein assay kits and <em>RC DC</em> protein assay kits 250 ml, alkaline copper tartrate solution for colorimetric assays; replacement reagent for <em>DC</em> protein assay kits and <em>RC DC</em> protein assay kits /webroot/web/images/lsr/products/sample_quantitation/sku_thumb/global/500-0113_tn.jpg /webroot/web/images/lsr/products/sample_quantitation/sku_view/global/500-0113_view.jpg /webroot/web/images/lsr/products/sample_quantitation/sku_feature/global/500-0113_feature.jpg preview RepEx 500-0113 250 ml, alkaline copper tartrate solution for colorimetric assays; replacement reagent for <em>DC</em> protein assay kits and <em>RC DC</em> protein assay kits 500-0113, 5000113, protein, assay, reagent, quantitation, concentration, reagents 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Sample-Quantitation/Protein-Assay-Kits-and-Cuvette N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000113&CartQuote=QT&quantity= true true false $58.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000113&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000113&quantity= 5000113
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Protein Assay Reagent B

5000114
1 L, dilute Folin reagent for colorimetric assays; replacement reagent for DC protein assay kits and RC DC protein assay kits

3 5000114 /templatedata/internet/product_SKU/data/LSR/Sample-Quantitation/Protein-Assay-Kits-and-Cuvette/500-0114_0921120600082.xml 5000114 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:09:00 5000114-protein-assay-reagent-b 5000114 Protein Assay Reagent B Protein Assay Reagent B 1 L, dilute Folin reagent for colorimetric assays; replacement reagent for <em>DC</em> protein assay kits and <em>RC DC</em> protein assay kits 1 L, dilute Folin reagent for colorimetric assays; replacement reagent for <em>DC</em> protein assay kits and <em>RC DC</em> protein assay kits 1 L, dilute Folin reagent for colorimetric assays; replacement reagent for <em>DC</em> protein assay kits and <em>RC DC</em> protein assay kits /webroot/web/images/lsr/products/sample_quantitation/sku_thumb/global/500-0114_tn.jpg /webroot/web/images/lsr/products/sample_quantitation/sku_view/global/500-0114_view.jpg /webroot/web/images/lsr/products/sample_quantitation/sku_feature/global/500-0114_feature.jpg preview RepEx 500-0114 1 L, dilute Folin reagent for colorimetric assays; replacement reagent for <em>DC</em> protein assay kits and <em>RC DC</em> protein assay kits 500-0114, 5000114, protein, assay, reagent, b, quantitation, concentration, reagents 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Sample-Quantitation/Protein-Assay-Kits-and-Cuvette N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000114&CartQuote=QT&quantity= false true false $58.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000114&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000114&quantity= 5000114
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Protein Assay Reagent S

5000115
5 ml, surfactant solution for colorimetric assays; replacement reagent for DC protein assay kits and RC DC protein assay kits

4 5000115 /templatedata/internet/product_SKU/data/LSR/Sample-Quantitation/Protein-Assay-Kits-and-Cuvette/500-0115_0921120600202.xml 5000115 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:09:01 5000115-protein-assay-reagent-s 5000115 Protein Assay Reagent S Protein Assay Reagent S 5 ml, surfactant solution for colorimetric assays; replacement reagent for <em>DC</em> protein assay kits and <em>RC DC</em> protein assay kits 5 ml, surfactant solution for colorimetric assays; replacement reagent for <em>DC</em> protein assay kits and <em>RC DC</em> protein assay kits 5 ml, surfactant solution for colorimetric assays; replacement reagent for <em>DC</em> protein assay kits and <em>RC DC</em> protein assay kits /webroot/web/images/lsr/products/sample_quantitation/sku_thumb/global/500-0115_tn.jpg /webroot/web/images/lsr/products/sample_quantitation/sku_view/global/500-0115_view.jpg /webroot/web/images/lsr/products/sample_quantitation/sku_feature/global/500-0115_feature.jpg preview RepEx 500-0115 5 ml, surfactant solution for colorimetric assays; replacement reagent for <em>DC</em> protein assay kits and <em>RC DC</em> protein assay kits 500-0115, 5000115, protein, assay, reagent, s, quantitation, concentration, reagents 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Sample-Quantitation/Protein-Assay-Kits-and-Cuvette N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000115&CartQuote=QT&quantity= false true false $39.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000115&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000115&quantity= 5000115
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DC Protein Assay Reagents Package

5000116
Reagent pack for colorimetric assays, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution; 450 assays

5 5000116 /templatedata/internet/product_SKU/data/LSR/Sample-Quantitation/Protein-Assay-Kits-and-Cuvette/500-0116_0921120600183.xml 5000116 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:09:01 5000116-dc-protein-assay-reagents-package 5000116 <em>DC</em> Protein Assay Reagents Package <em>DC</em> Protein Assay Reagents Package Reagent pack for colorimetric assays, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution; 450 assays Reagent pack for colorimetric assays, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution; 450 assays Reagent pack for colorimetric assays, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution; 450 assays /webroot/web/images/lsr/products/sample_quantitation/sku_thumb/global/500-0116_tn.jpg /webroot/web/images/lsr/products/sample_quantitation/sku_view/global/500-0116_view.jpg /webroot/web/images/lsr/products/sample_quantitation/sku_feature/global/500-0116_feature.jpg RepEx 500-0116 Reagent pack for colorimetric assays, includes 250 ml alkaline copper tartrate, 2 L dilute Folin reagent, 5 ml surfactant solution; 450 assays 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Sample-Quantitation/Protein-Assay-Kits-and-Cuvette N 0 5000116, dc, protein, concentration, assay, reagents, package, refill, colorimetric, assays, alkaline, copper, tartrate, folin, reagent, surfactant, solution false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000116&CartQuote=QT&quantity= true true false $191.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000116&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=5000116&quantity= 5000116
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Number Description Options
1123 Protein Assay Applications Bibliography Click to download
1069 Colorimetric Protein Assays, Rev D Click to download
1731 Bio-Rad DC Protein Assay Flier, Rev A. Click to download
LIT448 Instruction Manual, DC Protein Assay Click to download
1909 Modification of Bio-Rad DC Protein Assay for Use With Thiols Click to download
1770 Total Cellular Protein Determination Using the DC Protein Assay Click to download
6837 DC™ Protein Assay Quick Guide, Ver A Click to download
6852 Reagent Compatibility Chart for Bio-Rad Protein Assays Quick Guide,
Ver A
Click to download

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