Customized Process Columns

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Overview

Bio-Rad® InPlace Column Video
Bio-Rad InPlace process-scale chromatography columns are designed for industrial applications and allow contained filling, packing, unpacking, and cleaning in place for safe and hygienic column operation. To learn more about Bio-Rad InPlace columns, click on the video.
Bio-Rad EasyPack Column Video


Bio-Rad EasyPack columns are open columns with an adjustable bed-height adaptor and fixed bottom endpieces. Their sanitary design ensures hygienic performance and thorough cleaning. To learn more, click on the video.

InPlace Process Chromatography Column

Bio-Rad InPlace process-scale chromatography columns are designed for industrial applications and allow contained filling, packing, unpacking, and cleaning in place. The columns have a sanitary design, variable height adaptor, inflatable seal, and special low-shear valves for safe slurry transfer.

The Bio-Rad InPlace columns have been designed so that the packing, unpacking, and cleaning-in-place features do not interfere with the bed support, distribution system, and column tube, thus allowing optimum performance during any operation. These columns are directly scalable and retain the same general design from pilot to manufacturing scale. For instance, slurry valves can be added or removed to correlate with column diameter so that the efficiency of packing and unpacking in place is kept across the entire range of dimensions.

Bio-Rad InPlace columns are available in many standard sizes, and custom-made columns can be built in any tube height up to 1,200 mm or any diameter up to 2 m. The scale and scope of a project will seldom pose a constraint. Bio-Rad InPlace columns are available with glass, stainless steel, or acrylic column tubes.


  • Closed system design
  • Ideal for dense media
  • Adjustable height adaptor for axial compression
  • Designed for multiple packing methods
  • No resin loss during packing
  • Low mechanical shear during media transfer
  • Hoist-free operation with the Bio-Rad MainFrame lifting accessory
  • Complete documentation for regulatory submissions
  • Customizable diameter up to 2 m
Perform All Process Operations in a Self-Contained System to Save Buffers and Time with the Bio-Rad InPlace Process Chromatography column

Optimal Column Performance:

An All-In-Place System

The Bio-Rad InPlace column is designed to be operated with the Bio-Rad media transfer device to enable:

  • Cleaning in place of all wetted surfaces before column packing or unpacking
  • Even distribution of the slurry over the column diameter during transfer
  • Reslurrying in place before unpacking
  • Unpacking in place
  • Cleaning in place of the column
  • Maintenance in place using the Bio-Rad MainFrame lifting accessory

General Handling

To pack the column, the variable height adaptor is raised to its highest position, and slurried medium is pumped through the slurry valves into the column. The low-shear valves are specially designed to minimize damage to chromatography media during transfer. Buffer or slurry can be pumped through the slurry valves to maintain agitation, thereby reducing settling, if necessary. The column is packed by axial compression, flow or a combination of both, for optimal bed packing. The slurry valves can be rinsed while the column is packed to prepare for unpacking.

Before the unpacking operation begins, the bed is reslurried in the upflow/downflow mode while the piston is raised to its highest position. The slurry is then pumped out through the slurry valves, and when it is unloaded completely, the cleaning jets can be used to rinse the column. The powerful jets ensure that no medium or residue remains on any contact surface and help to sanitize the column.

Specifications
 
 
Bio-Rad InPlace Column Nominal Internal Diameter, mm*
   
 
400
450
600
800
1,000
1,200
1,400
 
Actual internal diameter, mm
400 ± 2
446 ± 2
596 ± 2
800 ± 2
1,000 ± 2
1,200 ± 2
1,400 ± 2
Motorization
Standard
Standard
Standard
Standard
Standard
Standard
Standard
Maximum pressure at 30°C, bar
Calibrated borosilicate glass
2.4
Stainless steel 316L**
6.0
6.0
6.0
3.0
3.0
3.0
3.0
Acrylic**
5.0
4.5
3.0
3.0
3.0
3.0
3.0
Floor space (L x W), mm
940 x 780
990 x 830
1,140 x 980
1,340 x 1,180
1,550 x 1,400
2,010 x 1,670
2,210 x 2,010
 
For tube length of 600 mm
Maximum column height, mm
1,810
1,810
1,850
1,860
1,890
2,370
2,430
Maximum usuable height in column, mm
490
490
510
490
510
600
600
Maximum bed height for 50% slurry, mm
245
245
255
245
255
300
300
Minimum bed height, mm
50
50
50
50
50
50
50
 
For tube length of 900 mm
Maximum column height, mm
2,110
2,110
2,150
2,160
2,190
2,670
2,730
Maximum usuable height in column, mm
790
790
810
790
810
900
900
Maximum bed height for 50% slurry, mm
395
395
405
395
405
450
450
Minimum bed height, mm
350
350
350
350
350
350
350
 

*    Columns with internal diameters >1,400 and <400 mm are available as special orders.
**  Higher pressure rating can be proposed as a special order.

Variable Height Adaptor and Optional Packing Motor

Bio-Rad InPlace columns use a variable height adaptor with an adjustable central screw mechanism. This design fits any column diameter from pilot to manufacturing scale. The adaptor allows either axial compression or flow packing, or both, while the central screw mechanism allows simple yet precise adjustment of the bed height.

Bio-Rad offers optional packing motors for all column diameters. A motor is strongly recommended for column diameters of 200–1,000 mm and is standard for columns >1,000 mm. The use of a Bio-Rad packing motor allows automatic movement (upward and downward) of the adaptor and controllable speed axial compression packing, which makes packing reproducible, easier, and faster.

Flow Distribution System

The adaptor and bottom endpiece of Bio-Rad InPlace columns comprise a unique flow distribution system that enhances packing and operational performance. The standard filter is stainless steel with 20 µm porosity for uniform flow and minimal product retention. The distributor plate directs the flow across the filter quickly and evenly. This combination of distributor plate and filter ensures optimal, uniform flow distribution across the bed and through the column at any flow rate.

Inflatable Seal

The design of the Bio-Rad InPlace adaptor seal provides added security against leakage and inefficient packing by ensuring uniform pressure throughout the seal. All columns have a pressure line through which the seal can be inflated with compressed gas (air or nitrogen). If preferred, a liquid pump can be installed so that inert fluids can be used for inflation instead of gas.

Regulatory Support and Quality Assurance

Bio-Rad's manufacturing procedures mandate full documentation of each step and every component in compliance with U.S. and European regulations. The original documentation and one electronic copy are included with each column. Bio-Rad maintains controlled copies. Bio-Rad InPlace chromatography columns are manufactured in compliance with engineering standards for pressure vessels, and each column comes with CE certification. The Bio-Rad manufacturing facility is certified according to ISO 9001:2000 standards. Manufacturing files for each project include:

  • Engineering drawings
  • Material traceability
  • Weld report and welder qualifications
  • Cleaning, electropolishing, and passivation certificates
  • User manual and maintenance instructions
  • Spare parts list
  • Documentation for equipment from other vendors
  • Factory acceptance test (FAT)
Packing CHT in a Bio-Rad InPlace Column

 

Bio-Rad InPlace process-scale chromatography columns are designed for industrial applications with contained filling, packing, unpacking, and cleaning for safe and hygienic column operation. To learn more about Bio-Rad InPlace columns, click on the video.

Optimized Performance When Using Bio-Rad EasyPack Column

 

Optimized Performance

 

Bio-Rad EasyPack process-scale chromatography columns are designed for industrial applications. These open columns have variable bed-height adaptors, inflatable seals, and fixed bottom endpieces. Their sanitary design ensures hygienic performance and thorough cleaning. All Bio-Rad EasyPack columns come with a complete set of regulatory support documentation.

From pilot to manufacturing scale, Bio-Rad EasyPack columns are directly scalable. To minimize scale-up and development time, these columns have the same basic design for all column configurations. Standard column dimensions include tube lengths of 300, 600, and 900 mm and diameters from 100 to 1,400 mm. Columns are available with borosilicate glass, 316L stainless steel, or acrylic column tubes. Columns may be customized to any length up to 1,200 mm or any diameter up to 2 m.

Variable Height Adaptor With Central Screw Adjustment Mechanism

Bio-Rad EasyPack columns have a centralized variable height adaptor for all column diameters up to 1,000 mm, from pilot to manufacturing scale. With its adjustable central screw mechanism, the adaptor allows either axial compression, flow packing, or both. Additionally, the adjustable adaptor provides the flexibility to accommodate any chromatography support at any bed height with a single column.

The adaptor design simplifies packing and unpacking. The central screw design allows a single operator to pack and unpack the column.

Unique Flow Distribution System

The adaptor and bottom endpiece of Bio-Rad EasyPack columns have a unique flow distribution system that enhances packing and operating performance and ensures uniform distribution at all flow rates.

The standard filter is stainless steel with a 20 µm porosity for uniform flow and minimal product retention. A distributor plate directs the flow across the filter quickly and evenly. This distributor plate and filter combination ensures optimal, uniform flow distribution across the bed and through the column.

Inflatable Seal Design

The design of the Bio-Rad EasyPack adaptor seal provides added security against leakage and poor packing by ensuring uniform pressure throughout the seal. The adaptor seals on all columns are inflated with compressed gas (air or nitrogen) as standard. If preferred, a liquid pump can be installed so that inert fluids can be used for inflation instead of gas.

Regulatory Support and Quality Assurance

The Bio-Rad manufacturing procedures ensure that each step and every component are fully documented. The qualification documentation package allows production in compliance with U.S. and European regulations. The original documentation and one electronic copy are included with each column. Bio-Rad maintains controlled copies. Bio-Rad EasyPack chromatography columns are manufactured in compliance with engineering standards for pressure vessels, and each column comes with CE certification. The Bio-Rad manufacturing facility is certified according to ISO 9001:2000 standards.

Bio-Rad Packing Motor

Bio-Rad offers an optional packing motor for all column diameters. The motor ensures quick and precise control when raising and lowering the adaptor. The packing motor makes it possible for one person to pack large-diameter columns. A motor is strongly recommended for column diameters of 200–1,000 mm and is standard for column diameters greater than 400 mm.

Customized Columns

In addition to offering a standard line of columns, Bio-Rad specializes in working with customers to design and manufacture custom columns. Customization may include modification to column dimensions, pressure rating, valves, ports, material, jacketing, etc. Bio-Rad EasyPack columns are easily upgradable to Bio-Rad InPlace process chromatography columns, which allow packing, unpacking, and cleaning in place. Please contact your local Bio-Rad sales representative for ordering information.

Specifications
 
 
Bio-Rad EasyPack Column Nominal Internal Diameter (mm)
   
 
200
300
350
400
450
600
800
1,000
 
Actual internal diameter, mm
200 ± 2
296 ± 2
350 ± 2
400 ± 2
446 ± 2
595.5 ± 2
800 ± 2
1,000 ± 3
Maximum pressure at 30°C, bar
Calibrated borosilicate glass
4.7
3.6
2.4
Stainless steel 316L
6.0
6.0
6.0
6.0
6.0
6.0
3.0
3.0
Acrylic
6.0
6.0
5.5
5.0
4.5
3.0
3.0
3.0
 

 

* Standard tube heights available: 600 and 900 mm.

Bio-Rad Media Transfer Device Video


The Bio-Rad media transfer device is a self-contained movable unit designed to transfer chromatography media between a tank and the column and to deliver cleaning solutions to Bio-Rad InPlace columns for sanitization. To learn more click on the video.

Bio-Rad Process Chromatography Skid 00 Video


Bio-Rad's process chromatography skid 00 is the industry's first compact skid designed for small-scale GMP biopharmaceutical manufacturing. The skid can be interchanged with multiple columns ranging in diameter from 70 to 300 mm with flow rates from 5 to 120 L/hr. To learn more click the video.

Process Chromatography Applications Laboratory

A Warm Welcome

Bio-Rad Laboratories welcomes you to our pilot-scale development laboratory for process chromatography. Located at Bio-Rad corporate headquarters in Hercules, California, on the southeastern shore of the San Pablo Bay, the laboratory offers access to state-of-the-art chromatography equipment and media and to the expertise of our team of separations scientists and engineers. Working with laboratory staff, you will have the opportunity to operate chromatography hardware, optimize your packing procedures, and review optimal solutions for your manufacturing processes.


To read more click here

Bio-Rad® MainFrame™ Lifting Accessory Video


The Bio-Rad MainFrame Lifting accessory enables hoist-free operation of chromatography columns. To learn more click on the video.

 

Bio-Rad specializes in working with customers to design and manufacture custom columns and liquid handling systems. Bio-Rad also partners with customers to provide an affordable and timely solution.

Customizable features include:

  • Column dimensions
  • Pressure rating
  • Number of valves
  • Number of ports
  • Construction material
  • Jacketing
  • Bubble trap volume
  • Pump size
  • Additional sensors (flow, pH, conductivity, temperature, pressure, and air)

Hardware is delivered with CE certification and is manufactured in our facility, which is certified according to ISO 9001:2000 standards. In Bio-Rad's manufacturing procedures, all steps and components are fully documented. The qualification documentation package assures production in compliance with U.S. and European regulations.

Number Description Options
5663 Bio-Rad InPlace Columns Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
5665 Bio-Rad EasyPack Columns Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
5739 Packing and Slurry-in-Place Procedures for CHT Ceramic Hydroxyapatite Using a Bio-Rad InPlace Process-Scale Chromatography Column, Rev A Click to download [ Add to Cart (Free) ]
5661 Bio-Rad Media Transfer Device, Rev B Click to download [ Add to Cart (Free) ]
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download
5659 Bio-Rad Mainframe Lifting Accessory Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
5488 State-of-the-Art Solutions for Process-Scale Chromatography, Rev C Click to download [ Add to Cart (Free) ]
5614 Bio-Rad Bubble Trap Product Information Sheet, Rev B Click to download
5615 Isolation Two-Way Valves Product Information Sheet, Rev A Click to download
5616 Liquid Pump for Inflatable Seals Product Information Sheet, Rev B Click to download
5617 Bio-Rad Packing Motor Product Information Sheet, Rev A Click to download
5620 Bio-Rad Media Slurry Tank Product Information Sheet, Rev A Click to download
5618 Pressure Gauge Product Information Sheet, Rev A Click to download
5619 Pressure Relief Valve Product Information Sheet, Rev A Click to download