Crosslinking Reagents and Catalysts

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Overview

Crosslinkers
  Formal Name Chemical Structure Applications
Bis N,N'-methylene-
bis-acrylamide
General crosslinker in PAGE
PDA Piperazine diacrylamide
Reduction of silver stain background in SDS-PAGE and 2-D gels, increased resolution, higher gel strength
DATD N,N,-diallyl-tartardiamide
Increased pore size of IEF gels, where molecular sieving is a problem. Used in scintillation counting: 1,2-dio structure is soluble in periodic acid
Acrylamide/Crosslinker Ratios
Ratio %C Common Applications
19:1 5 DNA sequencing
29:1 3.3 Protein separation
37.5:1 2.6 Protein separation

Bio-Rad offers standard crosslinkers (the widely used bis-acrylamide), alternative crosslinkers, and catalysts* for a variety of applications.

* For longer shelf life, store catalysts desiccated at room temperature.

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Bis Crosslinker

1610200
5 g, bis-acrylamide crosslinker

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$16.00
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Bis Crosslinker

1610201
50 g, bis-acrylamide crosslinker

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$50.00
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2% Bis Solution

1610142
500 ml, 2% bis-acrylamide solution

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$52.00
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2% Bis Solution

1610143
2 x 500 ml, 2% bis-acrylamide solution

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$92.00
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PDA Crosslinker

1610202
10 g, piperazine diacrylamide (PDA) crosslinker

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$125.00
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DATD Crosslinker

1610620
25 g, N,N'-diallyl-tartardiamide (DATD) crosslinker

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$82.00
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TEMED

1610800
5 ml, for catalyst for polyacrylamide gels

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$17.00
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TEMED

1610801
50 ml, catalyst for polyacrylamide gels; hazardous material shipping charges may apply

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$54.00
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Ammonium Persulfate (APS)

1610700
10 g, ammonium persulfate (APS) catalyst

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$14.00
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Riboflavin-5'-Phosphate

1610501
10 g, riboflavin-5'-phosphate catalyst

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$29.00
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Number Description Options
LIT300 Instruction Manual, Piperazine Diacrylamide (PDA), Rev B Click to download
2317 Ready-to-Run Buffers and Solutions Brochure, Rev F Click to download [ Add to Cart (Free) ]