Cross-linkers and Catalysts

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Overview

Crosslinkers
  Formal Name Chemical Structure Applications
Bis N,N'-methylene-
bis-acrylamide
General crosslinker in PAGE
PDA Piperazine diacrylamide
Reduction of silver stain background in SDS-PAGE and 2-D gels, increased resolution, higher gel strength
DATD N,N,-diallyl-tartardiamide
Increased pore size of IEF gels, where molecular sieving is a problem. Used in scintillation counting: 1,2-dio structure is soluble in periodic acid
Acrylamide/Crosslinker Ratios
Ratio %C Common Applications
19:1 5 DNA sequencing
29:1 3.3 Protein separation
37.5:1 2.6 Protein separation

Bio-Rad offers standard crosslinkers (the widely used bis-acrylamide), alternative crosslinkers, and catalysts* for a variety of applications.

* For longer shelf life, store catalysts desiccated at room temperature.

Bis Crosslinker

161-0200
5 g, bis-acrylamide crosslinker

List Price:   $16.00
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Bis Crosslinker

161-0201
50 g, bis-acrylamide crosslinker

List Price:   $48.00
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2% Bis Solution

161-0142
500 ml, 2% bis-acrylamide solution

List Price:   $50.00
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2% Bis Solution

161-0143
2 x 500 ml, 2% bis-acrylamide solution

List Price:   $88.00
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PDA Crosslinker

161-0202
10 g, piperazine diacrylamide (PDA) crosslinker

List Price:   $117.00
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DATD Crosslinker

161-0620
25 g, N,N'-diallyl-tartardiamide (DATD) crosslinker

List Price:   $79.00
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TEMED

161-0800
5 ml, for catalyst for polyacrylamide gels

List Price:   $17.00
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TEMED

161-0801
50 ml, catalyst for polyacrylamide gels; hazardous material shipping charges may apply

List Price:   $52.00
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Ammonium Persulfate (APS)

161-0700
10 g, ammonium persulfate (APS) catalyst

List Price:   $14.00
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Riboflavin-5'-Phosphate

161-0501
10 g, riboflavin-5'-phosphate catalyst

List Price:   $28.00
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Tributylphosphine (TBP)

163-2101
0.6 ml, 200 mM, reducing agent for use in protein sample preparation applications

List Price:   $41.00
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Number Description Options
LIT300 Instruction Manual, Piperazine Diacrylamide (PDA), Rev B Click to download
2317 Ready-to-Run Buffers and Solutions Brochure, Rev F Click to download [ Add to Cart (Free) ]