Criterion™ XT Tris-Acetate Precast Gels

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Criterion XT Tris-Acetate Precast Gels

Overview



Criterion™ XT gels are ideal for vertical midi protein gel electrophoresis, are designed to work with optimized sample and running buffers, and have an extended shelf life. Like traditional Laemmli systems, Criterion XT precast gels use discontinuous buffer ion fronts that form moving boundaries to stack and then separate proteins. Criterion XT Tris-acetate gels are formulated using a Tris-acetate buffer system (pH 7.0) that, when run with XT Tricine running buffer, separates large denatured proteins by molecular weight.

Features and Benefits

  • 8 month shelf life
  • Made without SDS, so they can also be used with nondenaturing sample and running buffers (native PAGE conditions) to separate proteins by mass-to-charge ratio
  • Nonreducing and nondenaturing native conditions of native PAGE preserve biological activity and can improve epitope detection by antibodies
  • Neutral pH significantly delays acrylamide hydrolysis as compared to traditional Laemmli systems
  • Run 2-D gels in less than a day

Applications and Uses

  • Polyacrylamide gel electrophoresis (PAGE or SDS-PAGE)
  • 2-D gel electrophoresis
  • Screen new samples
  • Evaluate sample preparation conditions

To further refine the resolution range, refer to the Criterion XT Tris-Acetate Gel chart.

Gel dimensions (W x L x thickness), cm 13.3 x 8.7 x 0.1
Cassette dimensions (W x L x thickness), cm 15.0 x 10.6 x .53
Cassette material Styrene copolymer
Comb material Polycarbonate
Storage tray material PET
Gel storage conditions Store flat at 2–8°C; do not freeze
  Gel shelf life at recommended temperature* 8 months
  Buffer volume, ml Upper, 60; lower, 400
Recommended sample buffer XT sample buffer + XT reducing agent
Recommended running buffer XT Tricine running buffer
  Gel chemistry Tris-acetate
  Gel format Midi
* From date of manufacture.  
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3–8% Criterion™ XT Tris-Acetate Protein Gel, 12+2 well, 45 µl

3450129
Pkg of 1, 3–8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

0 3450129 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/345-0129_0921120340508.xml 3450129 /default/main/bio-rad/WORKAREA/default 09/21/09 12:03 PM en_US jdl0t1yq 2018-02-01 00:22:50 true true internet/product_SKU jdl0t1yq templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/345-0129_0921120340508.xml LSR 2018-03-06 20:28:16 /default/main/bio-rad/STAGING N /default/main/bio-rad en 09/21/19 12:03 PM 1519716941748 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-03-06 20:28:16 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/345-0129_0921120340508.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/345-0129_0921120340508.xml 3450129-3-8-criterion-xt-tris-acetate-protein-gel-122-well-45-ul 3450129 Criterion XT Tris-Acetate Gel 3–8% Criterion™ XT Tris-Acetate Protein Gel, 12+2 well, 45 µl Pkg of 1, 3–8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells Pkg of 1, 3–8% polyacrylamide gel, 12+2 well, 45 μl, 13.3 x 8.7 cm (W x L), for use with Criterion and Criterion Dodeca cells Pkg of 1, 3–8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/345-0129_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/345-0129_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/345-0129_feature.jpg 3–8% Criterion™ XT Tris-Acetate Protein Gel, 12+2 well, 45 µl RepEx Criterion XT Tris-Acetate Gel #345-0129 Pkg of 1, 3–8% polyacrylamide gel, 12+2 well, 45 μl, 13.3 x 8.7 cm (W x L), for use with Criterion and Criterion Dodeca cells 345-0129, 3450129, 3-8%, criterion, xt, tris-acetate, gel, 12+2-well, 45, 12, tris, acetate, polyacrylamide, page, sds, sds-page, midi, precast, electrophoresis, western, blotting 09/21/09 12:03 PM 09/21/19 12:03 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Choose this versatile midi Criterion XT Tris-Acetate Gel for denaturing or nondenaturing PAGE. The gels are made without SDS, which allow the sample buffer and running buffer to determine the separation mechanism.</p> <ul> <li>For native PAGE, use Native Sample and Tris/Glycine Native Running Buffers</li> <li>For separation by molecular weight, use XT Sample and XT Tricine Running Buffers</li> <li>Neutral pH (pH 7.0) delays acrylamide hydrolysis as compared to traditional Laemmli systems</li> <li>8-month shelf life</li> <li>Separation range is ~40&ndash;400 kD under denaturing conditions</li> <li>Two wells available for markers or other references </li> <li>Can be loaded with a multichannel pipet</li> </ul> <p>Under denaturing conditions, with the SDS-containing Tricine running buffer, the discontinuous acetate and Tricine ion fronts form moving boundaries to stack and then separate proteins by molecular weight. During native electrophoresis, where proteins are separated by mass-to-charge ratio, discontinuous acetate and glycine ion fronts form moving boundaries to stack and separate proteins by both size and charge.</p> <h2 class="descHeader">Packaging Options</h2> <p><a href="/en-us/product/criterion-precast-gels/criterion-xt-tris-acetate-precast-gels">Criterion XT Tris-Acetate Midi Protein Gels</a> are available as 3&ndash;8% (separation range ~40&ndash;400 kD) or 7% (separation range ~35&ndash;250 kD) gels, with a selection of different well configurations.</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: left;" rowspan="2" valign="top"><strong>Well Configuration, Volume</strong></td> <td style="text-align: center;" colspan="2" valign="top"><strong>Catalog Number</strong></td> </tr> <tr> <td style="text-align: center;" valign="top"><strong>3&ndash;8%</strong></td> <td style="text-align: center;" valign="top"><strong>7%</strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top">12+2 well, 45 &micro;l</td> <td style="text-align: center;" valign="top">3450129</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450135-7-criterion-xt-tris-acetate-protein-gel-122-well-45-ul?ID=3450135">3450135</a></td> </tr> <tr> <td style="text-align: left;" valign="top">18 well, 30 &micro;l</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450130-3-8-criterion-xt-tris-acetate-protein-gel-18-well-30-ul?ID=3450130">3450130</a></td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450136-7-criterion-xt-tris-acetate-protein-gel-18-well-30-ul?ID=3450136">3450136</a></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top">26 well, 15 &micro;l</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450131-3-8-criterion-xt-tris-acetate-protein-gel-26-well-15-ul?ID=3450131">3450131</a></td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450137-7-criterion-xt-tris-acetate-protein-gel-26-well-15-ul?ID=3450137">3450137</a></td> </tr> <tr> <td style="text-align: left;" valign="top">11 cm IPG/prep+1 well, 550 &micro;l</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450133-3-8-criterion-xt-tris-acetate-protein-gel-11-cm-ipg-prep1-well-550-ul?ID=3450133">3450133</a></td> <td style="text-align: center;" valign="top">&ndash;</td> </tr> </tbody> </table> <h2 class="descHeader">Related Products</h2> <ul> <li>Native Sample Buffer (<a href="/en-us/sku/1610738-native-sample-buffer-for-protein-gels-30-ml?ID=1610738">1610738</a>)</li> <li>XT Sample Buffer, 4x, denaturing (<a href="/en-us/sku/1610791-xt-sample-buffer?ID=1610791">1610791</a>)</li> <li>Tris/Glycine Native Running Buffer, 10x (<a href="/en-us/sku/1610734-10x-tris-glycine-buffer-for-western-blots-native-gels?ID=1610734-native-running-buffer">1610734</a>)</li> <li>XT Tricine Running Buffer, 20x, denaturing (<a href="/en-us/sku/1610790-xt-tricine-running-buffer">1610790</a>)</li> <li>XT Reducing Agent, 20x (<a href="/en-us/sku/1610792-xt-reducing-agent">1610792</a>)</li> <li>XT Tricine Buffer Kit (<a href="/en-us/sku/1610797-xt-tricine-buffer-kit">1610797</a>), XT Tricine Running Buffer, XT Sample buffer, and XT Reducing Agent</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-ladders-standards-markers">Protein Ladders and Standards (Markers)</a> including unstained, prestained, and western standards</li> <li><a href="/en-us/product/criterion-vertical-electrophoresis-cell">Criterion Midi-Format Vertical Electrophoresis Cells</a>, for 1 to 4 gels</li> <li><a href="/en-us/product/electrophoresis-chambers/criterion-dodeca-cell">Criterion Dodeca Cell</a>, for up to 12 gels</li> <li><a href="/en-us/category/2-d-electrophoresis?ID=6916a228-0f95-4b26-a37a-5e4afe76add7">2-D Electrophoresis </a></li> <li><a href="/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452">Western Blotting</a></li> <li>ChemiDoc&trade; and GelDoc&trade; <a href="/en-us/category/gel-imaging-systems?ID=d6f93330-755b-410c-9c9c-77da24d51eab">Imaging Systems</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Criterion XT Precast Gel <a href="/webroot/web/pdf/lsr/literature/MS4110130B.pdf" target="_blank">Instruction Manual</a></li> <li>Criterion Gel <a href="/webroot/web/pdf/lsr/literature/4110001.pdf" target="_blank">Application Guide</a></li> <li>Criterion XT Precast Gels <a href="/webroot/web/pdf/lsr/literature/bulletin_2911.pdf" target="_blank">Product Information Sheet</a></li> <li>Criterion Precast Gel System <a href="/webroot/web/pdf/lsr/literature/Bulletin_2710.pdf" target="_blank">Brochure</a></li> <li>Electrophoresis Guide, <a href="/webroot/web/pdf/lsr/literature/Bulletin_6040.pdf" target="_blank">Interactive PDF</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B?LocaleKey=that">Protein Electrophoresis</a></li> <li><a href="/en-us/applications-technologies/introduction-western-blotting?ID=LUSPPAKG4">Western Blotting</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated/ 345-0129_0921120340508_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//345-0129_0921120340508_en.html Gel percentage 1689 3&ndash;8 0 0 Comb 1462 12+2-well 1 0 Sample volume, &micro;l 2121 45 1 0 Shelf life, months* 2710 8 1 0 Gel storage conditions 1693 Store flat at 2&ndash;8&deg;C; do not freeze 1 0 Recommended sample buffer 2065 XT sample buffer + XT reducing agent 1 0 Recommended running buffer 2064 XT Tricine running buffer 1 0 Gel dimensions (W x L x thickness), cm 1684 13.3 x 8.7 x 0.1 1 0 Cassette dimensions (W x L x thickness), cm 1413 15.0 x 10.6 x 0.53 1 0 Cassette material 1415 Styrene copolymer 1 0 Comb material 1464 Polycarbonate 1 0 Storage tray material 2213 PET 1 0 * From date of manufacture. 1096 &nbsp; 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3450129&CartQuote=QT&quantity= false true false $16.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3450129&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3450129&quantity= 3450129
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3–8% Criterion™ XT Tris-Acetate Protein Gel, 18 well, 30 µl

3450130
Pkg of 1, 3–8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

1 3450130 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/345-0130_0921120340438.xml 3450130 /default/main/bio-rad/WORKAREA/default 09/21/09 12:03 PM en_US jdl0t1ye 2018-02-01 00:22:50 true true internet/product_SKU jdl0t1ye templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/345-0130_0921120340438.xml LSR 2018-03-06 20:27:48 /default/main/bio-rad/STAGING N /default/main/bio-rad en 09/21/19 12:03 PM 1519716941791 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-03-06 20:27:48 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/345-0130_0921120340438.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/345-0130_0921120340438.xml 3450130-3-8-criterion-xt-tris-acetate-protein-gel-18-well-30-ul 3450130 Criterion XT Tris-Acetate Gel 3–8% Criterion™ XT Tris-Acetate Protein Gel, 18 well, 30 µl Pkg of 1, 3–8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells Pkg of 1, 3–8% polyacrylamide gel, 18-well, 30 μl, 13.3 x 8.7 cm (W x L), for use with Criterion and Criterion Dodeca cells Pkg of 1, 3–8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/345-0130_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/345-0130_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/345-0130_feature.jpg 3–8% Criterion™ XT Tris-Acetate Protein Gel, 18 well, 30 µl RepEx Criterion XT Tris-Acetate Gel #345-0130 Pkg of 1, 3–8% polyacrylamide gel, 18-well, 30 μl, 13.3 x 8.7 cm (W x L), for use with Criterion and Criterion Dodeca cells 345-0130, 3450130, 3-8%, criterion, xt, tris-acetate, gel, 18-well, 30, tris, acetate, polyacrylamide, page, sds, sds-page, midi, precast, electrophoresis, western, blotting 09/21/09 12:03 PM 09/21/19 12:03 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Choose this versatile midi Criterion XT Tris-Acetate Gel for denaturing or nondenaturing PAGE. The gels are made without SDS, which allow the sample buffer and running buffer to determine the separation mechanism.</p> <ul> <li>For native PAGE use Native Sample and Tris/Glycine Native Running Buffers</li> <li>For separation by molecular weight, use XT Sample and XT Tricine Running Buffers</li> <li>Neutral pH (pH 7.0) delays acrylamide hydrolysis as compared to traditional Laemmli systems</li> <li>8-month shelf life</li> <li>Separation range is ~40&ndash;400 kD under denaturing conditions</li> </ul> <p>Under denaturing conditions, with the SDS-containing Tricine running buffer, the discontinuous acetate and Tricine ion fronts form moving boundaries to stack and then separate proteins by molecular weight. During native electrophoresis, where proteins are separated by mass-to-charge ratio, discontinuous acetate and glycine ion fronts form moving boundaries to stack and separate proteins by both size and charge.</p> <h2 class="descHeader">Packaging Options</h2> <p><a href="/en-us/product/criterion-precast-gels/criterion-xt-tris-acetate-precast-gels">Criterion XT Tris-Acetate Midi Protein Gels</a> are available as 3&ndash;8% (separation range ~40&ndash;400 kD) or 7% (separation range ~35&ndash;250 kD) gels, with a selection of different well configurations.</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: left;" rowspan="2" valign="top"><strong>Well Configuration, Volume</strong></td> <td style="text-align: center;" colspan="2" valign="top"><strong>Catalog Number</strong></td> </tr> <tr> <td style="text-align: center;" valign="top"><strong>3&ndash;8%</strong></td> <td style="text-align: center;" valign="top"><strong>7%</strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top">12+2 well, 45 &micro;l</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450129-3-8-criterion-xt-tris-acetate-protein-gel-122-well-45-ul?ID=3450129">3450129</a></td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450135-7-criterion-xt-tris-acetate-protein-gel-122-well-45-ul?ID=3450135">3450135</a></td> </tr> <tr> <td style="text-align: left;" valign="top">18 well, 30 &micro;l</td> <td style="text-align: center;" valign="top">3450130</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450136-7-criterion-xt-tris-acetate-protein-gel-18-well-30-ul?ID=3450136">3450136</a></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top">26 well, 15 &micro;l</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450131-3-8-criterion-xt-tris-acetate-protein-gel-26-well-15-ul?ID=3450131">3450131</a></td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450137-7-criterion-xt-tris-acetate-protein-gel-26-well-15-ul?ID=3450137">3450137</a></td> </tr> <tr> <td style="text-align: left;" valign="top">11 cm IPG/prep+1 well, 550 &micro;l</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450133-3-8-criterion-xt-tris-acetate-protein-gel-11-cm-ipg-prep1-well-550-ul?ID=3450133">3450133</a></td> <td style="text-align: center;" valign="top">&ndash;</td> </tr> </tbody> </table> <h2 class="descHeader">Related Products</h2> <ul> <li>Native Sample Buffer (<a href="/en-us/sku/1610738-native-sample-buffer-for-protein-gels-30-ml?ID=1610738">1610738</a>)</li> <li>XT Sample Buffer, 4x, denaturing (<a href="/en-us/sku/1610791-xt-sample-buffer?ID=1610791">1610791</a>)</li> <li>Tris/Glycine Native Running Buffer, 10x (<a href="/en-us/sku/1610734-10x-tris-glycine-buffer-for-western-blots-native-gels?ID=1610734-native-running-buffer">1610734</a>)</li> <li>XT Tricine Running Buffer, 20x, denaturing (<a href="/en-us/sku/1610790-xt-tricine-running-buffer">1610790</a>)</li> <li>XT Reducing Agent, 20x (<a href="/en-us/sku/1610792-xt-reducing-agent">1610792</a>)</li> <li>XT Tricine Buffer Kit (<a href="/en-us/sku/1610797-xt-tricine-buffer-kit">1610797</a>), XT Tricine Running Buffer, XT Sample buffer, and XT Reducing Agent</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-ladders-standards-markers">Protein Ladders and Standards (Markers)</a> including unstained, prestained, and western standards</li> <li><a href="/en-us/product/criterion-vertical-electrophoresis-cell">Criterion Midi-Format Vertical Electrophoresis Cells</a>, for 1 to 4 gels</li> <li><a href="/en-us/product/electrophoresis-chambers/criterion-dodeca-cell">Criterion Dodeca Cell</a>, for up to 12 gels</li> <li><a href="/en-us/category/2-d-electrophoresis?ID=6916a228-0f95-4b26-a37a-5e4afe76add7">2-D Electrophoresis </a></li> <li><a href="/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452">Western Blotting</a></li> <li>ChemiDoc&trade; and GelDoc&trade; <a href="/en-us/category/gel-imaging-systems?ID=d6f93330-755b-410c-9c9c-77da24d51eab">Imaging Systems</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Criterion XT Precast Gel <a href="/webroot/web/pdf/lsr/literature/MS4110130B.pdf" target="_blank">Instruction Manual</a></li> <li>Criterion Gel <a href="/webroot/web/pdf/lsr/literature/4110001.pdf" target="_blank">Application Guide</a></li> <li>Criterion XT Precast Gels <a href="/webroot/web/pdf/lsr/literature/bulletin_2911.pdf" target="_blank">Product Information Sheet</a></li> <li>Criterion Precast Gel System <a href="/webroot/web/pdf/lsr/literature/Bulletin_2710.pdf" target="_blank">Brochure</a></li> <li>Electrophoresis Guide, <a href="/webroot/web/pdf/lsr/literature/Bulletin_6040.pdf" target="_blank">Interactive PDF</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B?LocaleKey=that">Protein Electrophoresis</a></li> <li><a href="/en-us/applications-technologies/introduction-western-blotting?ID=LUSPPAKG4">Western Blotting</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated/ 345-0130_0921120340438_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//345-0130_0921120340438_en.html Gel percentage 1689 3&ndash;8 0 0 Comb 1462 18-well 1 0 Sample volume, &micro;l 2121 30 1 0 Shelf life, months* 2710 8 1 0 Gel storage conditions 1693 Store flat at 2&ndash;8&deg;C; do not freeze 1 0 Recommended sample buffer 2065 XT sample buffer + XT reducing agent 1 0 Recommended running buffer 2064 XT Tricine running buffer 1 0 Gel dimensions (W x L x thickness), cm 1684 13.3 x 8.7 x 0.1 1 0 Cassette dimensions (W x L x thickness), cm 1413 15.0 x 10.6 x 0.53 1 0 Cassette material 1415 Styrene copolymer 1 0 Comb material 1464 Polycarbonate 1 0 Storage tray material 2213 PET 1 0 * From date of manufacture. 1096 &nbsp; 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3450130&CartQuote=QT&quantity= false true false $16.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3450130&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3450130&quantity= 3450130
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3–8% Criterion™ XT Tris-Acetate Protein Gel, 26 well, 15 µl

3450131
Pkg of 1, 3–8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

2 3450131 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/345-0131_0921120340488.xml 3450131 /default/main/bio-rad/WORKAREA/default 09/21/09 12:03 PM en_US jdl0t1yt 2018-02-01 00:22:50 true true internet/product_SKU jdl0t1yt templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/345-0131_0921120340488.xml LSR 2018-03-06 20:28:02 /default/main/bio-rad/STAGING N /default/main/bio-rad en 09/21/19 12:03 PM 1519716941833 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-03-06 20:28:02 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/345-0131_0921120340488.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/345-0131_0921120340488.xml 3450131-3-8-criterion-xt-tris-acetate-protein-gel-26-well-15-ul 3450131 Criterion XT Tris-Acetate Gel 3–8% Criterion™ XT Tris-Acetate Protein Gel, 26 well, 15 µl Pkg of 1, 3–8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells Pkg of 1, 3–8% polyacrylamide gel, 26-well, 15 μl, 13.3 x 8.7 cm (W x L), for use with Criterion and Criterion Dodeca cells Pkg of 1, 3–8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/345-0131_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/345-0131_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/345-0131_feature.jpg 3–8% Criterion™ XT Tris-Acetate Protein Gel, 26 well, 15 µl RepEx Criterion XT Tris-Acetate Gel #345-0131 Pkg of 1, 3–8% polyacrylamide gel, 26-well, 15 μl, 13.3 x 8.7 cm (W x L), for use with Criterion and Criterion Dodeca cells 345-0131, 3450131, 3-8%, criterion, xt, tris-acetate, gel, 26-well, 15, tris, acetate, polyacrylamide, page, sds, sds-page, midi, precast, electrophoresis, western, blotting 09/21/09 12:03 PM 09/21/19 12:03 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Choose this versatile midi Criterion XT Tris-Acetate Gel for denaturing or nondenaturing PAGE. The gels are made without SDS, which allow the sample buffer and running buffer to determine the separation mechanism.</p> <ul> <li>For native PAGE, use Native Sample and Tris/Glycine Native Running Buffers</li> <li>For separation by molecular weight use XT Sample and XT Tricine Running Buffers</li> <li>Neutral pH (pH 7.0) delays acrylamide hydrolysis as compared to traditional Laemmli systems</li> <li>8-month shelf life</li> <li>Separation range is ~40&ndash;400 kD under denaturing conditions</li> <li>Can be loaded with a multichannel pipet</li> </ul> <p>Under denaturing conditions, with the SDS-containing Tricine running buffer, the discontinuous acetate and Tricine ion fronts form moving boundaries to stack and then separate proteins by molecular weight. During native electrophoresis, where proteins are separated by mass-to-charge ratio, discontinuous acetate and glycine ion fronts form moving boundaries to stack and separate proteins by both size and charge.</p> <h2 class="descHeader">Packaging Options</h2> <p><a href="/en-us/product/criterion-precast-gels/criterion-xt-tris-acetate-precast-gels">Criterion XT Tris-Acetate Midi Protein Gels</a> are available as 3&ndash;8% (separation range ~40&ndash;400 kD) or 7% (separation range ~35&ndash;250 kD) gels, with a selection of different well configurations.</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: left;" rowspan="2" valign="top"><strong>Well Configuration, Volume</strong></td> <td style="text-align: center;" colspan="2" valign="top"><strong>Catalog Number</strong></td> </tr> <tr> <td style="text-align: center;" valign="top"><strong>3&ndash;8%</strong></td> <td style="text-align: center;" valign="top"><strong>7%</strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top">12+2 well, 45 &micro;l</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450129-3-8-criterion-xt-tris-acetate-protein-gel-122-well-45-ul?ID=3450129">3450129</a></td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450135-7-criterion-xt-tris-acetate-protein-gel-122-well-45-ul?ID=3450135">3450135</a></td> </tr> <tr> <td style="text-align: left;" valign="top">18 well, 30 &micro;l</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450130-3-8-criterion-xt-tris-acetate-protein-gel-18-well-30-ul?ID=3450130">3450130</a></td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450136-7-criterion-xt-tris-acetate-protein-gel-18-well-30-ul?ID=3450136">3450136</a></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top">26 well, 15 &micro;l</td> <td style="text-align: center;" valign="top">3450131</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450137-7-criterion-xt-tris-acetate-protein-gel-26-well-15-ul?ID=3450137">3450137</a></td> </tr> <tr> <td style="text-align: left;" valign="top">11 cm IPG/prep+1 well, 550 &micro;l</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450133-3-8-criterion-xt-tris-acetate-protein-gel-11-cm-ipg-prep1-well-550-ul?ID=3450133">3450133</a></td> <td style="text-align: center;" valign="top">&ndash;</td> </tr> </tbody> </table> <h2 class="descHeader">Related Products</h2> <ul> <li>Native Sample Buffer (<a href="/en-us/sku/1610738-native-sample-buffer-for-protein-gels-30-ml?ID=1610738">1610738</a>)</li> <li>XT Sample Buffer, 4x, denaturing (<a href="/en-us/sku/1610791-xt-sample-buffer?ID=1610791">1610791</a>)</li> <li>Tris/Glycine Native Running Buffer, 10x (<a href="/en-us/sku/1610734-10x-tris-glycine-buffer-for-western-blots-native-gels?ID=1610734-native-running-buffer">1610734</a>)</li> <li>XT Tricine Running Buffer, 20x, denaturing (<a href="/en-us/sku/1610790-xt-tricine-running-buffer">1610790</a>)</li> <li>XT Reducing Agent, 20x (<a href="/en-us/sku/1610792-xt-reducing-agent">1610792</a>)</li> <li>XT Tricine Buffer Kit (<a href="/en-us/sku/1610797-xt-tricine-buffer-kit">1610797</a>), XT Tricine Running Buffer, XT Sample buffer, and XT Reducing Agent</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-ladders-standards-markers">Protein Ladders and Standards (Markers)</a> including unstained, prestained, and western standards</li> <li><a href="/en-us/product/criterion-vertical-electrophoresis-cell">Criterion Midi-Format Vertical Electrophoresis Cells</a>, for 1 to 4 gels</li> <li><a href="/en-us/product/electrophoresis-chambers/criterion-dodeca-cell">Criterion Dodeca Cell</a>, for up to 12 gels</li> <li><a href="/en-us/category/2-d-electrophoresis?ID=6916a228-0f95-4b26-a37a-5e4afe76add7">2-D Electrophoresis </a></li> <li><a href="/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452">Western Blotting</a></li> <li>ChemiDoc&trade; and GelDoc&trade; <a href="/en-us/category/gel-imaging-systems?ID=d6f93330-755b-410c-9c9c-77da24d51eab">Imaging Systems</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Criterion XT Precast Gel <a href="/webroot/web/pdf/lsr/literature/MS4110130B.pdf" target="_blank">Instruction Manual</a></li> <li>Criterion Gel <a href="/webroot/web/pdf/lsr/literature/4110001.pdf" target="_blank">Application Guide</a></li> <li>Criterion XT Precast Gels <a href="/webroot/web/pdf/lsr/literature/bulletin_2911.pdf" target="_blank">Product Information Sheet</a></li> <li>Criterion Precast Gel System <a href="/webroot/web/pdf/lsr/literature/Bulletin_2710.pdf" target="_blank">Brochure</a></li> <li>Electrophoresis Guide, <a href="/webroot/web/pdf/lsr/literature/Bulletin_6040.pdf" target="_blank">Interactive PDF</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B?LocaleKey=that">Protein Electrophoresis</a></li> <li><a href="/en-us/applications-technologies/introduction-western-blotting?ID=LUSPPAKG4">Western Blotting</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated/ 345-0131_0921120340488_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//345-0131_0921120340488_en.html Gel percentage 1689 3&ndash;8 0 0 Comb 1462 26-well 1 0 Sample volume, &micro;l 2121 15 1 0 Shelf life, months* 2710 8 1 0 Gel storage conditions 1693 Store flat at 2&ndash;8&deg;C; do not freeze 1 0 Recommended sample buffer 2065 XT sample buffer + XT reducing agent 1 0 Recommended running buffer 2064 XT Tricine running buffer 1 0 Gel dimensions (W x L x thickness), cm 1684 13.3 x 8.7 x 0.1 1 0 Cassette dimensions (W x L x thickness), cm 1413 15.0 x 10.6 x 0.53 1 0 Cassette material 1415 Styrene copolymer 1 0 Comb material 1464 Polycarbonate 1 0 Storage tray material 2213 PET 1 0 * From date of manufacture. 1096 &nbsp; 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3450131&CartQuote=QT&quantity= false true false $16.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3450131&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3450131&quantity= 3450131
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Communication error

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3–8% Criterion™ XT Tris-Acetate Protein Gel, 11 cm IPG/prep+1 well, 550 µl

3450133
Pkg of 1, 3-8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells

6 3450133 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/345-0133_0921120340313.xml 3450133 /default/main/bio-rad/WORKAREA/default 09/21/09 12:03 PM en_US jdl0t1z3 2018-02-01 00:22:50 true true internet/product_SKU jdl0t1z3 templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/345-0133_0921120340313.xml LSR 2018-03-06 20:28:05 /default/main/bio-rad/STAGING N /default/main/bio-rad en 09/21/19 12:03 PM 1519716941876 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-03-06 20:28:05 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/345-0133_0921120340313.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/345-0133_0921120340313.xml 3450133-3-8-criterion-xt-tris-acetate-protein-gel-11-cm-ipg-prep1-well-550-ul 3450133 Criterion XT Tris-Acetate Gel 3–8% Criterion™ XT Tris-Acetate Protein Gel, 11 cm IPG/prep+1 well, 550 µl Pkg of 1, 3-8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells Pkg of 1, 3–8% polyacrylamide gel, IPG+1 well, 11 cm IPG strip, 13.3 x 8.7 cm (W x L), for use with Criterion and Criterion Dodeca cells Pkg of 1, 3-8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/345-0133_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/345-0133_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/345-0133_feature.jpg 3–8% Criterion™ XT Tris-Acetate Protein Gel, 11 cm IPG/prep+1 well, 550 µl RepEx Criterion XT Tris-Acetate Gel #345-0133 Pkg of 1, 3–8% polyacrylamide gel, IPG+1 well, 11 cm IPG strip, 13.3 x 8.7 cm (W x L), for use with Criterion and Criterion Dodeca cells 345-0133, 3450133, 3-8%, criterion, xt, tris-acetate, gel, ipg+1, 11, ipg/prep, 550, ipg, prep, immobilized, ph, gradient, tris, acetate, polyacrylamide, page, sds, sds-page, midi, precast, electrophoresis, western, blotting 09/21/09 12:03 PM 09/21/19 12:03 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Choose this versatile midi Criterion XT Tris-Acetate Gel for denaturing or nondenaturing PAGE. The gels are made without SDS, which allow the sample buffer and running buffer to determine the separation mechanism.</p> <ul> <li>For native PAGE, use Native Sample and Tris/Glycine Native Running Buffers</li> <li>For separation by molecular weight, use XT Sample and XT Tricine Running Buffers</li> <li>Neutral pH (pH 7.0) delays acrylamide hydrolysis as compared to traditional Laemmli systems </li> <li>8-month shelf life</li> <li>Run 2-D gels in less than a day</li> <li>Separation range is ~40&ndash;400 kD under denaturing conditions</li> <li>One well available for a marker or other reference</li> </ul> <p>Under denaturing conditions, with the SDS-containing Tricine running buffer, the discontinuous acetate and Tricine ion fronts form moving boundaries to stack and then separate proteins by molecular weight. During native electrophoresis, where proteins are separated by mass-to-charge ratio, discontinuous acetate and glycine ion fronts form moving boundaries to stack and separate proteins by both size and charge.</p> <h2 class="descHeader">Packaging Options</h2> <p><a href="/en-us/product/criterion-precast-gels/criterion-xt-tris-acetate-precast-gels">Criterion XT Tris-Acetate Midi Protein Gels</a> are available as 3&ndash;8% (separation range ~40&ndash;400 kD) or 7% (separation range ~35&ndash;250 kD) gels, with a selection of different well configurations.</p> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: left;" rowspan="2" valign="top"><strong>Well Configuration, Volume</strong></td> <td style="text-align: center;" colspan="2" valign="top"><strong>Catalog Number</strong></td> </tr> <tr> <td style="text-align: center;" valign="top"><strong>3&ndash;8%</strong></td> <td style="text-align: center;" valign="top"><strong>7%</strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top">12+2 well, 45 &micro;l</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450129-3-8-criterion-xt-tris-acetate-protein-gel-122-well-45-ul?ID=3450129">3450129</a></td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450135-7-criterion-xt-tris-acetate-protein-gel-122-well-45-ul?ID=3450135">3450135</a></td> </tr> <tr> <td style="text-align: left;" valign="top">18 well, 30 &micro;l</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450130-3-8-criterion-xt-tris-acetate-protein-gel-18-well-30-ul?ID=3450130">3450130</a></td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450136-7-criterion-xt-tris-acetate-protein-gel-18-well-30-ul?ID=3450136">3450136</a></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top">26 well, 15 &micro;l</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450131-3-8-criterion-xt-tris-acetate-protein-gel-26-well-15-ul?ID=3450131">3450131</a></td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/3450137-7-criterion-xt-tris-acetate-protein-gel-26-well-15-ul?ID=3450137">3450137</a></td> </tr> <tr> <td style="text-align: left;" valign="top">11 cm IPG/prep+1 well, 550 &micro;l</td> <td style="text-align: center;" valign="top">3450133</td> <td style="text-align: center;" valign="top">&ndash;</td> </tr> </tbody> </table> <h2 class="descHeader">Related Products</h2> <ul> <li>Native Sample Buffer (<a href="/en-us/sku/1610738-native-sample-buffer-for-protein-gels-30-ml?ID=1610738">1610738</a>)</li> <li>XT Sample Buffer, 4x, denaturing (<a href="/en-us/sku/1610791-xt-sample-buffer?ID=1610791">1610791</a>)</li> <li>Tris/Glycine Native Running Buffer, 10x (<a href="/en-us/sku/1610734-10x-tris-glycine-buffer-for-western-blots-native-gels?ID=1610734-native-running-buffer">1610734</a>)</li> <li>XT Tricine Running Buffer, 20x, denaturing (<a href="/en-us/sku/1610790-xt-tricine-running-buffer">1610790</a>)</li> <li>XT Reducing Agent, 20x (<a href="/en-us/sku/1610792-xt-reducing-agent">1610792</a>)</li> <li><a href="/en-us/product/readystrip-ipg-strips">ReadyStrip&trade; IPG Strips</a></li> <li><a href="/en-us/product/protean-i12-ief-system">PROTEAN<sup>&reg;</sup> i12&trade; IEF System</a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-ladders-standards-markers">Protein Ladders and Standards (Markers)</a> including unstained, prestained, western, IEF, and 2-D standards </li> <li><a href="/en-us/product/criterion-vertical-electrophoresis-cell">Criterion Midi-Format Vertical Electrophoresis Cells</a>, for 1 to 4 gels</li> <li><a href="/en-us/product/electrophoresis-chambers/criterion-dodeca-cell">Criterion Dodeca Cell</a>, for up to 12 gels</li> <li><a href="/en-us/category/2-d-electrophoresis?ID=6916a228-0f95-4b26-a37a-5e4afe76add7">2-D Electrophoresis </a></li> <li><a href="/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452">Western Blotting</a></li> <li>ChemiDoc&trade; and GelDoc&trade; <a href="/en-us/category/gel-imaging-systems?ID=d6f93330-755b-410c-9c9c-77da24d51eab">Imaging Systems</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Criterion XT Precast Gel <a href="/webroot/web/pdf/lsr/literature/MS4110130B.pdf" target="_blank">Instruction Manual</a></li> <li>Criterion Gel <a href="/webroot/web/pdf/lsr/literature/4110001.pdf" target="_blank">Application Guide</a></li> <li>Criterion XT Precast Gels <a href="/webroot/web/pdf/lsr/literature/bulletin_2911.pdf" target="_blank">Product Information Sheet</a></li> <li>Criterion Precast Gel System <a href="/webroot/web/pdf/lsr/literature/Bulletin_2710.pdf" target="_blank">Brochure</a></li> <li>Electrophoresis Guide, <a href="/webroot/web/pdf/lsr/literature/Bulletin_6040.pdf" target="_blank">Interactive PDF</a></li> <li>PROTEAN i12 IEF System <a href="/webroot/web/pdf/lsr/literature/Bulletin_6097A.pdf" target="_blank">Brochure</a></li> <li>ReadyStrip IPG Strip <a href="/webroot/web/pdf/lsr/literature/Bulletin_2442.pdf" target="_blank">Product Information Sheet</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B?LocaleKey=that">Protein Electrophoresis</a></li> <li><a href="/en-us/applications-technologies/2-d-electrophoresis-analysis">2-D Electrophoresis and Analysis</a></li> <li><a href="/en-us/applications-technologies/introduction-western-blotting?ID=LUSPPAKG4">Western Blotting</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated/ 345-0133_0921120340313_en.html /webroot/web/html/lsr/products/SKUs/autopopulated//345-0133_0921120340313_en.html Gel percentage 1689 3&ndash;8 0 0 Comb 1462 11 cm IPG+1-well 1 0 Shelf life, months* 2710 8 1 0 Gel storage conditions 1693 Store flat at 2&ndash;8&deg;C; do not freeze 1 0 Gel dimensions (W x L x thickness) (cm) 1684 13.3 x 8.7 x 0.1 1 0 Cassette dimensions (W x L x thickness) (cm) 1413 15.0 x 10.6 x 0.53 1 0 Cassette material 1415 Styrene copolymer 1 0 Comb material 1464 Polycarbonate 1 0 Storage tray material 2213 PET 1 0 * From date of manufacture. 1096 &nbsp; 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3450133&CartQuote=QT&quantity= false true false $16.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3450133&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=3450133&quantity= 3450133
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7% Criterion™ XT Tris-Acetate Protein Gel, 12+2 well, 45 µl

3450135
Discontinued item
Replaced with 3-8% Criterion XT Tris-Acetate Protein Gel, 12+2 well, 45 µl (3450129)

This product is not available for sale online. Please contact customer service for more information.
(Discontinued) 7% Criterion™ XT Tris-Acetate Protein Gel, 18 well, 30 µl

3450136
Discontinued item
Replaced with 3-8% Criterion XT Tris-Acetate Protein Gel, 18-well, 30 µl (3450130)

This product is no longer available. Please contact technical support for more information on replacement product.
7% Criterion™ XT Tris-Acetate Protein Gel, 26 well, 15 µl

3450137
Discontinued item
Replaced with 3-8% Criterion XT Tris-Acetate Protein Gel, 26-well, 15 µl (3450131)

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Mini-PROTEAN® Cassette Opening Lever

4560000
Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes

4560000 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/456-0000_1269895718.xml 4560000 /default/main/bio-rad/WORKAREA/default 03/29/10 11:02 AM en_US jcc1tp0p 2017-11-14 07:08:38 true true internet/product_SKU jcc1tp0p templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/456-0000_1269895718.xml LSR 2018-03-10 00:55:24 /default/main/bio-rad/STAGING N /default/main/bio-rad en 03/28/19 01:48 PM 1516780466081 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-03-10 00:55:24 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/456-0000_1269895718.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/456-0000_1269895718.xml 4560000-mini-protean-cassette-opening-lever 4560000 Mini-PROTEAN Cassette Opening Lever Mini-PROTEAN<sup>&#174;</sup> Cassette Opening Lever Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/456-0000_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/456-0000_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/456-0000_view.jpg Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Mini Format 1D-Electrophoresis Systems/Mini-PROTEAN Precast Gels/Mini-PROTEAN TGX Precast Gels ->MTS::KSHTFUE8Z## jHren Mini-PROTEAN Cassette Opening Lever Pkg of 1, opening lever for Mini-PROTEAN precast gel cassettes 4560000, mini-protean, cassette, opening, lever, opener, aluminum, recyclable, precast, gel, cassettes, protein, electrophoresis 03/29/10 11:02 AM 03/28/19 01:48 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 &nbsp; 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=4560000&CartQuote=QT&quantity= false true false $5.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=4560000&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=4560000&quantity= 4560000
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10x Tris/Glycine Buffer for Western Blots and Native Gels

1610734
Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

8 1610734 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0734_0921120339825.xml 1610734 /default/main/bio-rad/WORKAREA/default 09/21/09 12:03 PM en_US jcc1tp27 2017-11-14 07:08:21 true true internet/product_SKU jcc1tp27 templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0734_0921120339825.xml LSR 2018-03-06 20:28:19 /default/main/bio-rad/STAGING N /default/main/bio-rad en 09/21/19 12:03 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-03-06 20:28:19 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0734_0921120339825.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0734_0921120339825.xml 1610734-10x-tris-glycine-buffer-for-western-blots-native-gels 1610734 10x Tris/Glycine 10x Tris/Glycine Buffer for Western Blots and Native Gels Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, pH 8.3 Pkg of 1, 1 L, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0734_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0734_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0734_feature.jpg 10x Tris/Glycine Buffer for Western Blots and Native Gels RepEx 161-0734 1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, pH 8.3 161-0734, 1610734, 10x, tris/glycine, native, running, buffer, tris, glycine, polyacrylamide, page, protein, gel, electrophoresis, buffers, reagent, reagents 09/21/09 12:03 PM 09/21/19 12:03 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Use 10x Tris/Glycine Buffer as a transfer buffer for western blots or as a running buffer for native protein gel electrophoresis.</p> <ul> <li>For tank or semi-dry blotting for SDS PAGE gels, usually with the addition of 20% methanol</li> <li>For tank blotting of native gels, without methanol</li> <li>As a running buffer for native gels</li> </ul> <h2 class="descHeader">Precast gels that can be used for native electrophoresis</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: left;" width="87" valign="top"><strong>Precast Gel Type</strong></td> <td style="text-align: center;" width="87" valign="top"><strong>Size</strong></td> <td style="text-align: left;" width="87" valign="top"><strong>Gel Chemistry</strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/mini-protean-precast-gels/mini-protean-tgx-precast-gels">Mini-PROTEAN<sup>&reg;</sup> TGX&trade;</a></td> <td style="text-align: center;" valign="top">Mini</td> <td style="text-align: left;" rowspan="2" valign="top">Laemmli-like, long shelf life, fast separation with high resolution</td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/protein-gels/criterion-precast-gels/criterion-tgx-precast-gels">Criterion&trade; TGX&trade;</a></td> <td style="text-align: center;" valign="top">Midi</td> </tr> <tr> <td style="text-align: left;" valign="top"><a href="/en-us/product/mini-protean-precast-gels/mini-protean-tgx-stain-free-precast-gels">Mini-PROTEAN<sup>&reg;</sup> TGX Stain-Free&trade;</a></td> <td style="text-align: center;" valign="top">Mini</td> <td style="text-align: left;" rowspan="2" valign="top">Laemmli-like, long shelf life, fast separation with high resolution, unique trihalo compounds for rapid fluorescent protein detection</td> </tr> <tr> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-precast-gels/criterion-tgx-stain-free-precast-gels">Criterion&trade; TGX Stain-Free&trade;</a></td> <td style="text-align: center;" valign="top">Midi</td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-tris-hcl-precast-gels">Criterion&trade; Tris-HCl</a></td> <td style="text-align: center;" valign="top">Midi</td> <td style="text-align: left;" valign="top">Standard Laemmli</td> </tr> <tr> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-precast-gels/criterion-stain-free-tris-hcl-gels">Criterion Stain Free&trade; Tris-HCl</a></td> <td style="text-align: center;" valign="top">Midi</td> <td style="text-align: left;" valign="top">Standard Laemmli, unique trihalo compounds for rapid fluorescent protein detection</td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-precast-gels/criterion-xt-tris-acetate-precast-gels">Criterion XT Tris-Acetate</a></td> <td style="text-align: center;" valign="top">Midi</td> <td style="text-align: left;" valign="top">Discontinuous buffer ion fronts form moving boundaries to stack, and then separate proteins</td> </tr> </tbody> </table> <p class="caption" style="margin-top:-10px">Note: Most proteins have an acidic or slightly basic pI (~3&ndash;8) and are run with the power supply connected to the electrophoresis chamber as for SDS-PAGE. If more basic proteins (pl &gt;8.5) of interest are being separated, change the polarity of the electrodes, since they have a net positive charge.</p> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: center;" width="87" valign="top"><strong>10x Buffer Volume</strong></td> <td style="text-align: center;" width="87" valign="top"><strong>Catalog Number </strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: center;" valign="top">1 L</td> <td style="text-align: center;" valign="top">1610734</td> </tr> <tr> <td style="text-align: center;" valign="top">5 L cube</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1610771-10x-tris-glycine-buffer-for-western-blots-native-gels?ID=1610771">1610771</a></td> </tr> </tbody> </table> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/precisionab-validated-western-blotting-antibodies">PrecisionAb&trade; Validated Western Blotting Antibodies</a></li> <li>Native Sample Buffer (<a href="//en-us/sku/1610738-native-sample-buffer-for-protein-gels-30-ml?ID=1610738">1610738</a>)</li> <li>Criterion Staining/Blotting Trays with lids (<a href="/en-us/sku/3459921-criterion-staining-blotting-trays">3459921</a>)</li> <li>10x Phosphate Buffered Saline (PBS) (<a href="/en-us/sku/1610780-10x-phosphate-buffered-saline-pbs?ID=1610780">1610780</a>)</li> <li>10x Tris Buffered Saline (TBS) (<a href="/en-us/sku/1706435-10x-tris-buffered-saline-tbs?ID=1706435-tbs">1706435</a>)</li> <li>1x Phosphate Buffered Saline (PBS) with 1% Casein (<a href="/en-us/sku/1610783-1x-phosphate-buffered-saline-pbs-with-1-casein">1610783</a>)</li> <li>1x Tris Buffered Saline (TBS) with 1% Casein (<a href="/en-us/sku/1610782-1x-tris-buffered-saline-tbs-with-1-casein?ID=1610782">1610782</a>)</li> <li>Blotting-Grade Blocker, nonfat dry milk (<a href="/en-us/sku/1706404-blotting-grade-blocker">1706404</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-gels">Protein Gels</a></li> <li><a href="/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452-membranes-filter-paper">Western Blotting Membranes and Filter Paper</a></li> <li><a href="/en-us/category/semi-dry-rapid-blotting-systems">Semi-Dry and Rapid Blotting Systems</a></li> <li><a href="/en-us/category/wet-tank-blotting-systems">Wet/Tank Blotting Systems</a></li> <li><a href="/en-us/category/power-supplies?ID=5f7dc809-5983-461f-90c9-4610c7d6f780">Power Supplies</a></li> <li><a href="/en-us/category/transfer-buffers">Transfer Buffers</a></li> <li><a href="/en-us/category/immunodetection-reagents-kits?ID=534c009d-cd86-4b33-956c-674d5a6ae054">Immunodetection Reagents and Kits</a></li> <li><a href="/en-us/category/gel-imaging-systems?ID=d6f93330-755b-410c-9c9c-77da24d51eab">Gel and Western Imaging Systems</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Mini-PROTEAN Precast Gels <a href="/webroot/web/pdf/lsr/literature/Bulletin_1658100.pdf" target="_blank">Instruction Manual and Application Guide</a></li> <li>Criterion Gel Application Guide <a href="/webroot/web/pdf/lsr/literature/4110001.pdf" target="_blank">Instruction Manual</a></li> <li>Mini Trans-Blot<sup>&reg;</sup> Cell <a href="/webroot/web/pdf/lsr/literature/M1703930.pdf" target="_blank">Instruction Manual</a></li> <li>Trans-Blot SD Cell <a href="/webroot/web/pdf/lsr/literature/4006066B.pdf" target="_blank">Quick Reference Guide</a></li> <li>Trans-Blot SD Semi-Dry Transfer Cell <a href="/webroot/web/pdf/lsr/literature/M1703940.pdf" target="_blank">Instruction Manual</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B">Protein Electrophoresis</a></li> <li><a href="/en-us/applications-technologies/introduction-western-blotting?ID=LUSPPAKG4">Western Blotting</a></li> <li><a href="/en-us/applications-technologies/imaging-analysis">Imaging and Analysis</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1610734.html /webroot/web/html/lsr/products/SKUs/autopopulated/1610734.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610734&CartQuote=QT&quantity= false true false $29.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610734&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610734&quantity= 1610734
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Native Sample Buffer for Protein Gels, 30 ml

1610738
30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue

1610738 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0738_0921120339091.xml 1610738 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jcrucy9rjcrucy9s" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jcrucy9rjcrucy9s"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="24" DayOfWeek="3" Year="2018" Hour="21" Minute="59" Second="57" ZoneOffsetMillis="0" Time="1516831197001">Wed Jan 24 21:59:57 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0738_0921120339091.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="24" DayOfWeek="3" Year="2018" Hour="21" Minute="59" Second="57" ZoneOffsetMillis="0" Time="1516831197001">Wed Jan 24 21:59:57 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:03 PM en_US jcc1tp0g 2017-11-14 07:08:21 true true internet/product_SKU LSR N en 09/21/19 12:03 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-02-01 00:22:43 1610738-native-sample-buffer-for-protein-gels-30-ml 1610738 Native Sample Buffer Native Sample Buffer for Protein Gels, 30 ml 30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue 30 ml, premixed protein sample buffer for native PAGE 30 ml, premixed protein sample buffer for native PAGE, contains 62.5 mM Tris-HCl, pH 6.8, 40% glycerol, 0.01% bromophenol blue /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0738_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0738_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0738_feature.jpg Native Sample Buffer for Protein Gels, 30 ml preview RepEx 161-0738 30 ml, premixed protein sample buffer for native PAGE 161-0738, 1610738, native, sample, buffer, premixed, protein, page, polyacrylamide, gel, electrophoresis, loading 09/21/09 12:03 PM 09/21/19 12:03 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Use Native Sample Buffer to retain native protein structure and mass-to-charge ratios during protein electrophoresis. This sample buffer is nondenaturing, containing no SDS, and has no reducing agent. The mobility of proteins in native gels depends on a number of factors in addition to molecular weight, including protein shape and charge.</p> <ul> <li>The biological activity of most proteins is retained</li> <li>Some protein-protein interactions may be maintained</li> <li>Native gel electrophoresis cannot be used for molecular weight estimation</li> <li>Since migration is influenced by structure, proteins of the same size can often be easily resolved</li> <li>Dry samples can be dissolved directly into the sample buffer</li> </ul> <h2 class="descHeader">Precast gels that can be used for native electrophoresis</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: left;" width="87" valign="top"><strong>Precast Gels</strong></td> <td style="text-align: center;" width="87" valign="top"><strong>Size</strong></td> <td style="text-align: left;" width="87" valign="top"><strong>Gel Chemistry</strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/mini-protean-precast-gels/mini-protean-tgx-precast-gels">Mini-PROTEAN<sup>&reg;</sup> TGX&trade;</a></td> <td style="text-align: center;" valign="top">Mini</td> <td style="text-align: left;" valign="top">Laemmli-like, long shelf life, fast separation with high resolution</td> </tr> <tr> <td style="text-align: left;" valign="top"><a href="/en-us/product/protein-gels/criterion-precast-gels/criterion-tgx-precast-gels">Criterion&trade; TGX&trade; </a></td> <td style="text-align: center;" valign="top">Midi</td> <td style="text-align: left;" valign="top">Laemmli-like, long shelf life, fast separation with high resolution</td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/mini-protean-precast-gels/mini-protean-tgx-stain-free-precast-gels">Mini-PROTEAN<sup>&reg;</sup> TGX Stain-Free&trade;</a></td> <td style="text-align: center;" valign="top">Mini</td> <td style="text-align: left;" valign="top">Laemmli-like, long shelf life, fast separation with high resolution, unique trihalo compounds for rapid fluorescent protein detection</td> </tr> <tr> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-precast-gels/criterion-tgx-stain-free-precast-gels%20?ID=N3GSCDHYP">Criterion&trade; TGX Stain-Free&trade; </a></td> <td style="text-align: center;" valign="top">Midi</td> <td style="text-align: left;" valign="top">Laemmli-like, long shelf life, fast separation with high resolution, unique trihalo compounds for rapid fluorescent protein detection</td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-tris-hcl-precast-gels">Criterion&trade; Tris-HCl </a></td> <td style="text-align: center;" valign="top">Midi</td> <td style="text-align: left;" valign="top">Standard Laemmli</td> </tr> <tr> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-precast-gels/criterion-stain-free-tris-hcl-gels">Criterion Stain Free&trade; Tris-HCl </a></td> <td style="text-align: center;" valign="top">Midi</td> <td style="text-align: left;" valign="top">Standard Laemmli, unique trihalo compounds for rapid fluorescent protein detection</td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-precast-gels/criterion-xt-tris-acetate-precast-gels">Criterion XT Tris-Acetate </a></td> <td style="text-align: center;" valign="top">Midi</td> <td style="text-align: left;" valign="top">Discontinuous buffer ion fronts form moving boundaries to stack, and then separate proteins</td> </tr> </tbody> </table> <br /> <br /> <p class="caption" style="margin-top:-20px">Note: Most proteins have an acidic or slightly basic pI (~3&ndash;8) and are run with the power supply connected to the electrophoresis chamber as for SDS-PAGE. If more basic proteins (pl &gt;8.5) are of interest, change the polarity of the electrodes, since they have a net positive charge.</p> <p class="caption" style="margin-top:-20px">&nbsp;</p> <h2 class="descHeader">Related Products</h2> <ul> <li>10x Tris/Glycine (<a href="/en-us/sku/1610734-10x-tris-glycine-buffer-for-western-blots-native-gels?ID=1610734">1610734</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/product/running-buffers-reagents?ID=f86a065f-68a3-48ca-bbac-567f2beea5d5">Running Buffers and Reagents</a></li> <li><a href="/en-us/product/sample-loading-buffers-reagents?ID=09e4dbf1-40c5-4a92-ace5-3ec31296ac7e">Sample Loading Buffers and Reagents</a></li> <li><a href="/en-us/category/electrophoresis-chambers">Electrophoresis Chambers</a></li> <li><a href="/en-us/category/power-supplies?ID=5f7dc809-5983-461f-90c9-4610c7d6f780">Power Supplies</a></li> <li><a href="/en-us/category/protein-stains">Protein Stains</a></li> <li><a href="/en-us/category/gel-imaging-systems?ID=d6f93330-755b-410c-9c9c-77da24d51eab">Gel Imaging Systems</a></li> </ul> <h2 class="descHeader">Supporting Documentation</h2> <ul> <li>Native Sample Buffer <a href="/webroot/web/pdf/lsr/literature/4006027F.pdf" target="_blank">Instruction Manual</a></li> <li>Mini-PROTEAN Precast Gels <a href="/webroot/web/pdf/lsr/literature/Bulletin_1658100.pdf" target="_blank">Instruction Manual and Application Guide</a></li> <li>Criterion Gel Application Guide <a href="/webroot/web/pdf/lsr/literature/4110001.pdf">Instruction Manual</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B">Protein Electrophoresis</a></li> <li><a href="/en-us/applications-technologies/introduction-western-blotting?ID=LUSPPAKG4">Western Blotting</a></li> <li><a href="/en-us/applications-technologies/imaging-analysis">Imaging and Analysis</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1610738.html /webroot/web/html/lsr/products/SKUs/autopopulated/1610738.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610738&CartQuote=QT&quantity= false true false $16.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610738&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610738&quantity= 1610738
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10x Tris/Glycine Buffer for Western Blots and Native Gels

1610771
Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water

10 1610771 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0771_0921120339765.xml 1610771 /default/main/bio-rad/WORKAREA/default 09/21/09 12:03 PM en_US jcc1toym 2017-11-14 07:08:21 true true internet/product_SKU jcc1toym templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0771_0921120339765.xml LSR 2018-03-06 20:28:04 /default/main/bio-rad/STAGING N /default/main/bio-rad en 09/21/19 12:03 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-03-06 20:28:04 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0771_0921120339765.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0771_0921120339765.xml 1610771-10x-tris-glycine-buffer-for-western-blots-native-gels 1610771 10x Tris/Glycine 10x Tris/Glycine Buffer for Western Blots and Native Gels Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water 5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, pH 8.3 Pkg of 1, 5 L cube, 10x premixed electrophoresis buffer contains 25 mM Tris, 192 mM glycine, pH 8.3 following dilution to 1x with water /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0771_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0771_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0771_feature.jpg 10x Tris/Glycine Buffer for Western Blots and Native Gels RepEx 161-0771 5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, pH 8.3 161-0771, 1610771, 10x, tris/glycine, native, running, buffer, tris, glycine, polyacrylamide, page, protein, gel, electrophoresis, buffers, reagent, reagents 09/21/09 12:03 PM 09/21/19 12:03 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>Use 10x Tris/Glycine Buffer as a transfer buffer for western blots or as a running buffer for native protein gel electrophoresis.</p> <ul> <li>For tank or semi-dry blotting for SDS PAGE gels, usually with the addition of 20% methanol</li> <li>For tank blotting of native gels, without methanol</li> <li>As a running buffer for native gels</li> </ul> <h2 class="descHeader">Precast gels that can be used for native electrophoresis</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: left;" width="87" valign="top"><strong>Precast Gel Type</strong></td> <td style="text-align: center;" width="87" valign="top"><strong>Size</strong></td> <td style="text-align: left;" width="87" valign="top"><strong>Gel Chemistry</strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/mini-protean-precast-gels/mini-protean-tgx-precast-gels">Mini-PROTEAN<sup>&reg;</sup> TGX&trade;</a></td> <td style="text-align: center;" valign="top">Mini</td> <td style="text-align: left;" rowspan="2" valign="top">Laemmli-like, long shelf life, fast separation with high resolution</td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/protein-gels/criterion-precast-gels/criterion-tgx-precast-gels">Criterion&trade; TGX&trade;</a></td> <td style="text-align: center;" valign="top">Midi</td> </tr> <tr> <td style="text-align: left;" valign="top"><a href="/en-us/product/mini-protean-precast-gels/mini-protean-tgx-stain-free-precast-gels">Mini-PROTEAN<sup>&reg;</sup> TGX Stain-Free&trade;</a></td> <td style="text-align: center;" valign="top">Mini</td> <td style="text-align: left;" rowspan="2" valign="top">Laemmli-like, long shelf life, fast separation with high resolution, unique trihalo compounds for rapid fluorescent protein detection</td> </tr> <tr> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-precast-gels/criterion-tgx-stain-free-precast-gels">Criterion&trade; TGX Stain-Free&trade;</a></td> <td style="text-align: center;" valign="top">Midi</td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-tris-hcl-precast-gels">Criterion&trade; Tris-HCl</a></td> <td style="text-align: center;" valign="top">Midi</td> <td style="text-align: left;" valign="top">Standard Laemmli</td> </tr> <tr> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-precast-gels/criterion-stain-free-tris-hcl-gels">Criterion Stain Free&trade; Tris-HCl</a></td> <td style="text-align: center;" valign="top">Midi</td> <td style="text-align: left;" valign="top">Standard Laemmli, unique trihalo compounds for rapid fluorescent protein detection</td> </tr> <tr class="pd_colorbackground"> <td style="text-align: left;" valign="top"><a href="/en-us/product/criterion-precast-gels/criterion-xt-tris-acetate-precast-gels">Criterion XT Tris-Acetate</a></td> <td style="text-align: center;" valign="top">Midi</td> <td style="text-align: left;" valign="top">Discontinuous buffer ion fronts form moving boundaries to stack, and then separate proteins</td> </tr> </tbody> </table> <p class="caption" style="margin-top:-10px">Note: Most proteins have an acidic or slightly basic pI (~3&ndash;8) and are run with the power supply connected to the electrophoresis chamber as for SDS-PAGE. If more basic proteins (pl &gt;8.5) of interest are being separated, change the polarity of the electrodes, since they have a net positive charge.</p> <h2 class="descHeader">Packaging Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td style="text-align: center;" width="87" valign="top"><strong>10x Buffer Volume</strong></td> <td style="text-align: center;" width="87" valign="top"><strong>Catalog Number </strong></td> </tr> <tr class="pd_colorbackground"> <td style="text-align: center;" valign="top">1 L</td> <td style="text-align: center;" valign="top"><a href="/en-us/sku/1610734-10x-tris-glycine-buffer-for-western-blots-native-gels?ID=1610734">1610734</a></td> </tr> <tr> <td style="text-align: center;" valign="top">5 L cube</td> <td style="text-align: center;" valign="top">1610771</td> </tr> </tbody> </table> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/precisionab-validated-western-blotting-antibodies">PrecisionAb&trade; Validated Western Blotting Antibodies</a></li> <li>Native Sample Buffer (<a href="/en-us/sku/1610738-native-sample-buffer-for-protein-gels-30-ml?ID=1610738">1610738</a>)</li> <li>Criterion Staining/Blotting Trays with lids (<a href="/en-us/sku/3459921-criterion-staining-blotting-trays">3459921</a>)</li> <li>10x Phosphate Buffered Saline (PBS) (<a href="/en-us/sku/1610780-10x-phosphate-buffered-saline-pbs?ID=1610780">1610780</a>)</li> <li>10x Tris Buffered Saline (TBS) (<a href="/en-us/sku/1706435-10x-tris-buffered-saline-tbs?ID=1706435-tbs">1706435</a>)</li> <li>1x Phosphate Buffered Saline (PBS) with 1% Casein (<a href="/en-us/sku/1610783-1x-phosphate-buffered-saline-pbs-with-1-casein">1610783</a>)</li> <li>1x Tris Buffered Saline (TBS) with 1% Casein (<a href="/en-us/sku/1610782-1x-tris-buffered-saline-tbs-with-1-casein?ID=1610782">1610782</a>)</li> <li>Blotting-Grade Blocker, nonfat dry milk (<a href="/en-us/sku/1706404-blotting-grade-blocker">1706404</a>)</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li><a href="/en-us/category/protein-gels">Protein Gels</a></li> <li><a href="/en-us/category/western-blotting?ID=9324fd3c-6af4-4551-831e-8db6fa3f3452-membranes-filter-paper">Western Blotting Membranes and Filter Paper</a></li> <li><a href="/en-us/category/semi-dry-rapid-blotting-systems">Semi-Dry and Rapid Blotting Systems</a></li> <li><a href="/en-us/category/wet-tank-blotting-systems">Wet/Tank Blotting Systems</a></li> <li><a href="/en-us/category/power-supplies?ID=5f7dc809-5983-461f-90c9-4610c7d6f780">Power Supplies</a></li> <li><a href="/en-us/category/transfer-buffers">Transfer Buffers</a></li> <li><a href="/en-us/category/immunodetection-reagents-kits?ID=534c009d-cd86-4b33-956c-674d5a6ae054">Immunodetection Reagents and Kits</a></li> <li><a href="/en-us/category/gel-imaging-systems?ID=d6f93330-755b-410c-9c9c-77da24d51eab">Gel and Western Imaging Systems</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Mini-PROTEAN Precast Gels <a href="/webroot/web/pdf/lsr/literature/Bulletin_1658100.pdf" target="_blank">Instruction Manual and Application Guide</a></li> <li>Criterion Gel Application Guide <a href="/webroot/web/pdf/lsr/literature/4110001.pdf" target="_blank">Instruction Manual</a></li> <li>Mini Trans-Blot<sup>&reg;</sup> Cell <a href="/webroot/web/pdf/lsr/literature/M1703930.pdf" target="_blank">Instruction Manual</a></li> <li>Trans-Blot SD Cell <a href="/webroot/web/pdf/lsr/literature/4006066B.pdf" target="_blank">Quick Reference Guide</a></li> <li>Trans-Blot SD Semi-Dry Transfer Cell <a href="/webroot/web/pdf/lsr/literature/M1703940.pdf" target="_blank">Instruction Manual</a></li> </ul> <h2 class="descHeader">Online Applications</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-protein-electrophoresis?ID=LUSOVO47B">Protein Electrophoresis</a></li> <li><a href="/en-us/applications-technologies/introduction-western-blotting?ID=LUSPPAKG4">Western Blotting</a></li> <li><a href="/en-us/applications-technologies/imaging-analysis">Imaging and Analysis</a></li> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated 1610771.html /webroot/web/html/lsr/products/SKUs/autopopulated/1610771.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610771&CartQuote=QT&quantity= false true false $99.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610771&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610771&quantity= 1610771
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XT Tricine Running Buffer

1610790
Pkg of 1, 500 ml, 20x electrophoresis buffer for use with Criterion™ XT Tris-Acetate Gels

1610790 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0790_0921120342794.xml 1610790 09/21/09 12:03 PM N en 09/21/19 12:03 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:21 1610790-xt-tricine-running-buffer 1610790 XT Tricine Running Buffer XT Tricine Running Buffer Pkg of 1, 500 ml, 20x electrophoresis buffer for use with Criterion&trade; XT Tris-Acetate Gels 500 ml, 20x electrophoresis buffer for use with Criterion XT Tris-Acetate gels Pkg of 1, 500 ml, 20x electrophoresis buffer for use with Criterion&trade; XT Tris-Acetate Gels /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0790_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0790_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0790_feature.jpg preview RepEx 161-0790 500 ml, 20x electrophoresis buffer for use with Criterion XT Tris-Acetate gels 1610790, xt, tricine, running, buffer, 20x, gel, electrophoresis, criterion, tris-acetate, gels 09/21/09 12:03 PM 09/21/19 12:03 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610790&CartQuote=QT&quantity= false true false $66.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610790&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610790&quantity= 1610790
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XT Sample Buffer

1610791
10 ml, 4x premixed protein sample buffer for use with all Criterion XT gels

1610791 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0791_0921120342467.xml 1610791 /default/main/bio-rad/WORKAREA/default 09/21/09 12:03 PM en_US jebrbib8 2018-02-28 05:02:34 true true internet/product_SKU jebrbib8 templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0791_0921120342467.xml LSR 2018-03-06 20:27:36 /default/main/bio-rad/STAGING N /default/main/bio-rad en 09/21/19 12:03 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2018-03-06 20:27:36 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0791_0921120342467.xml templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0791_0921120342467.xml 1610791-xt-sample-buffer 1610791 XT Sample Buffer XT Sample Buffer 10 ml, 4x premixed protein sample buffer for use with all Criterion XT gels 10 ml, 4x premixed protein sample buffer for use with all Criterion XT gels 10 ml, 4x premixed protein sample buffer for use with all Criterion XT gels /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0791_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0791_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0791_feature.jpg RepEx 161-0791 10 ml, 4x premixed protein sample buffer for use with all Criterion XT gels 161-0791, 1610791, xt, sample, buffer, loading, premixed, protein, gel, electrophoresis, reagents, criterion, sds, page 09/21/09 12:03 PM 09/21/19 12:03 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 <p>XT sample buffer is added to protein samples during SDS-PAGE separation when using MOPS, MES, or Tricine running buffer.</p> <ul> <li>For use with dilute and concentrated samples</li> <li>Premixed loading buffer &mdash; ensures lane-to-lane consistency</li> <li>Quality-controlled reagent &mdash; guarantees reproducible results</li> </ul> <h2 class="descHeader">Related Products</h2> <p>We offer additional <a href="/evportal/destination/commerce/product_detail?catID=09e4dbf1-40c5-4a92-ace5-3ec31296ac7e">sample buffers and reagents</a> for protein electrophoresis including:</p> <ul> <li>Laemmli sample buffer &mdash; <a href="/en-us/sku/1610737-2x-laemmli-sample-buffer?ID=1610737">2x (161-0737)</a> or <a href="/en-us/sku/1610747-4x-laemmli-sample-buffer?ID=1610747">4x (161-0747)</a></li> <li><a href="/en-us/sku/1610738-native-sample-buffer-for-protein-gels-30-ml?ID=1610738">Native sample buffer (161-0738)</a></li> <li><a href="/en-us/sku/1610739-tricine-sample-buffer-for-protein-gels-30-ml?ID=1610739">Tricine sample buffer (161-0739)</a></li> <li><a href="/en-us/sku/1610763-ief-sample-buffer-for-vertical-gels-30-ml?ID=1610763">IEF sample buffer (161-0763)</a></li> <li><a href="/en-us/sku/1610764-zymogram-sample-buffer-30-ml?ID=1610764">Zymogram sample buffer (161-0764)</a></li> </ul> /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global xt-sample-buffer-616-0791-desc.html /webroot/web/html/lsr/products/electrophoresis/sku_view/description/global/xt-sample-buffer-616-0791-desc.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610791&CartQuote=QT&quantity= false true false $13.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610791&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610791&quantity= 1610791
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XT Reducing Agent

1610792
1 ml, 20x reducing agent for use with all Criterion XT gels

1610792 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0792_0921120342551.xml 1610792 09/21/09 12:03 PM N en 09/21/19 12:03 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:21 1610792-xt-reducing-agent 1610792 XT Reducing Agent XT Reducing Agent 1 ml, 20x reducing agent for use with all Criterion XT gels 1 ml, 20x reducing agent for use with all Criterion XT gels 1 ml, 20x reducing agent for use with all Criterion XT gels /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0792_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0792_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0792_feature.jpg preview RepEx 161-0792 1 ml, 20x reducing agent for use with all Criterion XT gels 161-0792, 1610792, xt, reducing, agent, protein, gel, electrophoresis, buffers, reagent, reagents, criterion, gels 09/21/09 12:03 PM 09/21/19 12:03 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610792&CartQuote=QT&quantity= true true false $24.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610792&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610792&quantity= 1610792
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XT Tricine Buffer Kit

1610797
Pkg of 1, electrophoresis buffer kit, includes 500 ml 20x XT Tricine Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml 20x XT Reducing Agent

1610797 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl/161-0797_0921120342755.xml 1610797 09/21/09 12:03 PM N en 09/21/19 12:03 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:21 1610797-xt-tricine-buffer-kit 1610797 XT Tricine Buffer Kit XT Tricine Buffer Kit Pkg of 1, electrophoresis buffer kit, includes 500 ml 20x XT Tricine Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml 20x XT Reducing Agent Electrophoresis buffer kit, includes 500 ml 20x XT Tricine running buffer, 10 ml 4x XT sample buffer, 1 ml 20x XT reducing agent Pkg of 1, electrophoresis buffer kit, includes 500 ml 20x XT Tricine Running Buffer, 10 ml 4x XT Sample Buffer, 1 ml 20x XT Reducing Agent /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/161-0797_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0797_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/161-0797_feature.jpg preview RepEx 161-0797 Electrophoresis buffer kit, includes 500 ml 20x XT Tricine running buffer, 10 ml 4x XT sample buffer, 1 ml 20x XT reducing agent 1610797, xt, tricine, buffer, 20x, running, electrophoresis, gel, criterion, sample, buffers, reducing, agent 09/21/09 12:03 PM 09/21/19 12:03 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Protein-Electrophoresis-and-Bl N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610797&CartQuote=QT&quantity= false true false $101.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610797&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1610797&quantity= 1610797
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Number Description Options
2788 Criterion Blotting Tray Insert, Rev A Click to download
2912 Criterion XT Precast Gel Flier, Rev A Click to download
2710 Criterion™ Precast Gel System Brochure, Rev C Click to download
2911 Criterion XT Precast Gels Product Information Sheet, Rev B Click to download
4110001 Instruction Manual, Criterion™ Gel Application Guide, Ver H Click to download
4110130 Instruction Manual, Criterion XT Precast Gel Instruction Guide, Rev B Click to download

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