Criterion XT Tris-Acetate Precast Gels

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Overview

Criterion XT Tris-Acetate Gels

Criterion™ XT gels are ideal for vertical midi gel electrophoresis and are designed to work with optimized sample and running buffers without the need for antioxidant addition. Like traditional Laemmli systems, Criterion XT precast gels use discontinuous buffer ion fronts that form moving boundaries to stack and then separate proteins. Criterion XT Tris-acetate gels are formulated using a Tris-acetate buffer system (pH 7.0) that, when run with XT Tricine running buffer, separates large denatured proteins by molecular weight.

Features and Benefits

  • 8 month shelf life
  • Made without SDS, so they can also be used with nondenaturing sample and running buffers (native PAGE conditions) to separate proteins by mass-to-charge ratio
  • Nonreducing and nondenaturing native conditions of native PAGE preserve biological activity and can improve epitope detection by antibodies
  • Neutral pH significantly delays acrylamide hydrolysis as compared to traditional Laemmli systems
  • Run 2-D gels in less than a day

Applications and Uses

  • Polyacrylamide gel electrophoresis (PAGE or SDS-PAGE)
  • 2-D gel electrophoresis
  • Screen new samples
  • Evaluate sample preparation conditions

To further refine the resolution range, refer to the Criterion XT Tris-Acetate Gel chart.

Gel dimensions (W x L x thickness), cm 13.3 x 8.7 x 0.1
Cassette dimensions (W x L x thickness), cm 15.0 x 10.6 x .53
Cassette material Styrene copolymer
Comb material Polycarbonate
Storage tray material PET
Gel storage conditions Store flat at 2–8°C; do not freeze
  Gel shelf life at recommended temperature* 8 months
  Buffer volume, ml Upper, 60; lower, 400
Recommended sample buffer XT sample buffer + XT reducing agent
Recommended running buffer XT Tricine running buffer
  Gel chemistry Tris-acetate
  Gel format Midi
* From date of manufacture.  
Criterion XT Tris-Acetate Gel #345-0129
3–8% Criterion™ XT Tris-Acetate Gel, 12+2 well, 45 µl

345-0129
Pkg of 1, 3–8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Tris-Acetate Gel #345-0130
3–8% Criterion™ XT Tris-Acetate Gel, 18 well, 30 µl

345-0130
Pkg of 1, 3–8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Tris-Acetate Gel #345-0131
3–8% Criterion™ XT Tris-Acetate Gel, 26 well, 15 µl

345-0131
Pkg of 1, 3–8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Tris-Acetate Gel #345-0135
7% Criterion™ XT Tris-Acetate Gel, 12+2 well, 45 µl

345-0135
Pkg of 1, 7% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Tris-Acetate Gel #345-0136
7% Criterion™ XT Tris-Acetate Gel, 18 well, 30 µl

345-0136
Pkg of 1, 7% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Tris-Acetate Gel #345-0137
7% Criterion™ XT Tris-Acetate Gel, 26 well, 15 µl

345-0137
Pkg of 1, 7% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Tris-Acetate Gel #345-0133
3–8% Criterion™ XT Tris-Acetate Gel, IPG+1 well, 11 cm IPG/prep well, 550 µl

345-0133
Pkg of 1, 3-8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Number Description Options
2788 Criterion Blotting Tray Insert, Rev A Click to download
2912 Criterion XT Precast Gel Flier, Rev A Click to download
2710 Criterion Precast Gel System Brochure, Rev C Click to download
2911 Criterion XT Precast Gels Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
4110001 Instruction Manual, Criterion Gel Application Guide, Rev F Click to download
4110130 Instruction Manual, Criterion XT Precast Gel Instruction Guide, Rev B Click to download