Criterion XT Bis-Tris Precast Gels

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Overview

Criterion XT Migration Chart for Bis-Tris

The banding patterns indicate the optimal separation ranges (in kD) for each acrylamide percentages in combinations with the specified buffer system.

Criterion™ XT gels are ideal for vertical midi gel electrophoresis and are designed to work with optimized sample and running buffers without the need for antioxidant addition. Like traditional Laemmli systems, Criterion XT gels use discontinuous buffer ion fronts that form moving boundaries to stack and then separate proteins.

Features and Benefits

  • 12 month shelf life
  • Formulated using a Bis-Tris-HCl buffer system (pH 6.4) to separate proteins by molecular weight
  • Select from two running buffers to expand the separation capability of a single Bis-Tris gel type
  • Near-neutral pH significantly delays acrylamide hydrolysis as compared to traditional Laemmli systems
  • Run 2-D gels in less than a day

Applications and Uses

  • Polyacrylamide gel electrophoresis (PAGE or SDS-PAGE)
  • 2-D gel electrophoresis
  • Screen new samples
  • Evaluate sample preparation conditions

To further refine the resolution range, refer to the Protein Migration Chart for different acrylamide concentrations appropriate for your proteins of interest.

Gel dimensions (W x L x thickness) 13.3 x 8.7 x 0.1 cm
Cassette dimensions (W x L x thickness) 15.0 x 10.6 x 5.3 cm
Cassette material Styrene copolymer
Comb material Polycarbonate
Storage tray material PET
Gel storage conditions Store flat at room temperature; do not freeze
  Gel shelf life at recommended temperature* 12 months
Buffer volume Upper, 60 ml; lower, 400 ml
Recommended sample buffer XT sample buffer + XT reducing agent
Recommended running buffer XT MES or XT MOPS
  Gel chemistry Bis-Tris
Gel format Midi
* From date of manufacture.  
Criterion XT Bis-Tris Gel #345-0123
4–12% Criterion™ XT Bis-Tris Gel, 12+2 well, 45 µl

345-0123
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Bis-Tris Gel #345-0124
4–12% Criterion™ XT Bis-Tris Gel, 18 well, 30 µl

345-0124
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Bis-Tris Gel #345-0125
4–12% Criterion™ XT Bis-Tris Gel, 26 well, 15 µl

345-0125
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Bis-Tris Gel #345-0126
4–12% Criterion™ XT Bis-Tris Gel, Prep+2 well, 700 µl

345-0126
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Bis-Tris Gel #345-0111
10% Criterion™ XT Bis-Tris Gel, 12+2 well, 45 µl

345-0111
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Bis-Tris Gel #345-0112
10% Criterion™ XT Bis-Tris Gel, 18 well, 30 µl

345-0112
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Bis-Tris Gel #345-0113
10% Criterion™ XT Bis-Tris Gel, 26 well, 15 strip

345-0113
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Bis-Tris Gel #345-0117
12% Criterion™ XT Bis-Tris Gel, 12+2 well, 45 µl

345-0117
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Bis-Tris Gel #345-0118
12% Criterion™ XT Bis-Tris Gel, 18 well, 30 µl

345-0118
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Bis-Tris Gel #345-0119
12% Criterion™ XT Bis-Tris Gel, 26 well, 15 µl

345-0119
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Bis-Tris Gel #345-0120
12% Criterion™ XT Bis-Tris Gel, Prep+2 well, 700 µl

345-0120
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Bis-Tris Gel #345-0127
4–12% Criterion™ XT Bis-Tris Gel, IPG+1 well, 11 cm IPG/prep well, 550 µl

345-0127
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Bis-Tris Gel #345-0115
10% Criterion™ XT Bis-Tris Gel, IPG+1 well, 11 cm IPG/prep well, 550 µl

345-0115
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Bis-Tris Gel #345-0121
12% Criterion™ XT Bis-Tris Gel, IPG+1 well, 11 cm IPG/prep well, 550 µl

345-0121
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Number Description Options
6040 Electrophoresis Guide, Interactive PDF, Rev B Click to download
2788 Criterion Blotting Tray Insert, Rev A Click to download
2912 Criterion XT Precast Gel Flier, Rev A Click to download
2710 Criterion Precast Gel System Brochure, Rev C Click to download
2911 Criterion XT Precast Gels Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
4110001 Instruction Manual, Criterion Gel Application Guide, Rev F Click to download
4110130 Instruction Manual, Criterion XT Precast Gel Instruction Guide, Rev B Click to download