Criterion Precast Gels

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Overview

Migration Pattern for Criterion

The banding patterns indicate the optimal separation ranges (in bp) for each acrylamide percentage in combinations with the specified buffer system. Standard protein migration on Ready Gel Tris-HCl precast gels (above). The molecular weight of each band is given in kD. Migrations are based on the leading dye front being run to the bottom of a gel.

IPG + 1 Well Gels

IPG+1 well gels accommodate 11 cm ReadyStrip IPG strips plus an SDS-PAGE standard for 2-D electrophoresis applications.

The precast gels available for the Criterion™ system are 13.3 x 8.7 cm polyacrylamide gels in single-use cassettes. This gel size provides reproducible, high-resolution results with fast setup, loading, and run times.

For 2-D applications, the Criterion IPG combs are designed to hold 11 cm ReadyStrip™ IPG strips, which provide 35% more focusing area in the first-dimension separation and 20% more gel area in the second dimension than mini systems using 7 cm strips. A sample well for protein standards is also included.

Criterion precast gels for 2-D applications are available in TGX™, TGX Stain-Free™, Bis-Tris, Tris-acetate, and Tris-HCl formulations.

The new Criterion™ TGX™ (Tris-Glycine eXtended) precast gels for PAGE are based on the long shelf life TGX formulation. The TGX gels retain Laemmli-like separation characteristics using the standard sample and Tris/glycine running buffers. The proteins in the gel can be separated in as little as 20 min at 300 V while maintaining low temperatures.

TGX and Tris-HCl gels are manufactured without SDS. These gels can be used to run proteins under nondenaturing conditions for subsequent analysis of their native conformation and activity by omitting SDS from the running buffer. SDS is added to the sample and running buffer to create standard SDS denaturing electrophoresis conditions.

Criterion gels are packaged and sold in individual units: 1–2 gels can be run in the Criterion cell and 1–12 gels can be run in the high-throughput Criterion™ Dodeca™ cell.

Gel dimensions (Width x Length x Thickness) 133 x 87 x 1 mm
Cassette dimensions (Width x Length x Thickness) 150 x 106 x 53 mm
Cassette material Styrene copolymer
Comb material Polycarbonate
Gel storage conditions Store flat at 4°C; do not freeze
Shelf life at 2-8°C* 12 months
Recommended sample buffer (dilute 1:1 with sample) Laemmli sample buffer: 62.5mM Tris-HCl, pH 6.8, 2% SDS, 25% Glycerol, 0.01% Bromophenol Blue
Recommended running buffer Tris/Glycine/SDS running buffer: 25mM Tris, 192mM Glycine, 0.1% SDS, pH 8.3
Run times at constant voltage: 200 V 42-50 min
Run times at constant voltage: 300 V 20-26 min

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Criterion XT Bis-Tris Gel #345-0127
4–12% Criterion™ XT Bis-Tris Gel, 11 cm IPG/prep+1 well, 550 µl

3450127
Pkg of 1, 4–12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Bis-Tris Gel #345-0115
10% Criterion™ XT Bis-Tris Gel, 11 cm IPG/prep+1 well, 550 µl

3450115
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Bis-Tris Gel #345-0121
12% Criterion™ XT Bis-Tris Gel, 11 cm IPG/prep+1 well, 550 µl

3450121
Pkg of 1, 12% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion XT Tris-Acetate Gel #345-0133
3–8% Criterion™ XT Tris-Acetate Gel, 11 cm IPG/prep+1 well, 550 µl

3450133
Pkg of 1, 3-8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion Tris-HCl Gel #345-0101
10% Criterion™ Tris-HCl Gel, 11 cm IPG/prep+1 well, 550 µl

3450101
Pkg of 1, 10% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion Tris-HCl Gel #345-0106
10.5–14% Criterion™ Tris-HCl Gel, IPG+1 well, 11 cm IPG/prep well, 550 µl

3450106
Pkg of 1, 10.5–14% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion Tris-HCl Gel #345-0107
10–20% Criterion™ Tris-HCl Gel, 11 cm IPG/prep+1 well, 550 µl

3450107
Pkg of 1, 10–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion Tris-HCl Gel #345-0102
12.5% Criterion™ Tris-HCl Gel, 11 cm IPG/prep+1 well, 550 µl

3450102
Pkg of 1, 12.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion Tris-HCl Gel #345-0103
4–15% Criterion™ Tris-HCl Gel, 11 cm IPG/prep+1 well, 550 µl

3450103
Pkg of 1, 4–15% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion Tris-HCl Gel #345-0105
8–16% Criterion™ Tris-HCl Gel, 11 cm IPG/prep+1 well, 550 µl

3450105
Pkg of 1, 8–16% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Criterion Tris-HCl Gel #345-0104
4–20% Criterion™ Tris-HCl Gel, 11 cm IPG/prep+1 well, 550 µl

3450104
Pkg of 1, 4–20% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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Number Description Options
2788 Criterion Blotting Tray Insert, Rev A Click to download
2710 Criterion™ Precast Gel System Brochure, Rev C Click to download
5981 Criterion™ Tris-HCl to Criterion™ TGX™ and TGX Stain-Free™ Precast Gels Catalog Number Conversion Chart, Rev B Click to download [ Add to Cart (Free) ]
4110001 Instruction Manual, Criterion™ Gel Application Guide, Rev F Click to download