Criterion Tris-Tricine Precast Gels

Tris-Tricine gels are ideal for separation of peptides and small proteins with a molecular weight <10,000. Superior resolution is achieved by slowing the migration rate of the peptide-SDS complexes.

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Criterion Tris-Tricine Precast Gels

Ordering Information

Overview

Criterion Tris-Tricine precast gels are ideal for vertical midi gel electrophoresis of peptides and small proteins with a molecular weight <10,000 D. Superior resolution is achieved by slowing the migration rate of the peptide-SDS complexes. This helps achieve separation from faster-moving SDS micelles that interfere with peptide resolution in Tris-glycine buffer systems.

Features and Benefits

Ideal for the separation of small molecular–weight proteins
Reproducible, high-resolution results
Fast setup, loading, and run times
Numbered, marked well outlines on the cassette ease sample loading and identification
Run 2-D gels in less than a day
Robust system allows separation of complex samples

Applications and Uses

Polyacrylamide gel electrophoresis (PAGE or SDS-PAGE)
2-D gel electrophoresis
Accurate molecular weight estimation

Use the Protein Migration Chart to select a gel percentage that will provide optimal resolution of your sample.

Related Products

Specifications

Gel dimensions (W x L x thickness), cm

13.3 x 8.7 x 0.1

Cassette dimensions (W x L x thickness), cm

15.0 x 10.6 x .53

Cassette material

Styrene copolymer

Comb material

Polycarbonate

Storage tray material

PET

Gel storage conditions

Store flat at 2–8°C; do not freeze

Gel shelf life at recommended temperature*

12 weeks

Buffer volume, ml

Upper, 60; lower, 400

Recommended sample buffer

Tricine Sample Buffer (1610739); dilute at least 1:1 with sample

Gel chemistry

Tris-HCl

* From date of manufacture.

Ordering

items

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(Discontinued) 16.5% Criterion™ Tris-Tricine Gel, 12+2 well, 45 µl

3450063

Pkg of 1, 16.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

(Discontinued) 16.5% Criterion™ Tris-Tricine Gel, 18 well, 30 µl

3450064

Pkg of 1, 16.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

(Discontinued) 16.5% Criterion™ Tris-Tricine Gel, 26 well, 15 µl

3450065

Pkg of 1, 16.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

Accessories

(Discontinued) Mini-PROTEAN^® Cassette Opening Lever

4560000

Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes

(Discontinued) Gel Releasers

1653320

Pkg of 5, gel releaser for Mini-PROTEAN^® Tetra system, Mini-PROTEAN^® 3 Dodeca™ cell, Mini-PROTEAN 3 multi-casting chamber, and PROTEAN^® Plus Dodeca™ cell

Kits & Reagents

(Discontinued) 10x Tris/Tricine/SDS Running Buffer, 1 L

1610744

Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 100 mM Tris, 100 mM Tricine, 0.1% SDS, pH 8.3 following dilution to 1x with water

(Discontinued) Tricine Sample Buffer for Protein Gels, 30 ml

1610739

30 ml, premixed protein sample buffer for peptide and small protein SDS-PAGE, contains 200 mM Tris-HCl, pH 6.8, 40% glycerol, 2% SDS, 0.04% Coomassie Blue G-250