Coomassie Stains

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Overview

Bio-Safe Coomassie Stain

Protein stained with Bio-Safe Coomassie stain. Gel contains SDS-PAGE low range standards (lanes 1, 8), human transferrin (lanes 2, 3), fish lysate (lanes 4, 5), and E. coli lysate (lanes 6, 7).

Bio-Safe Coomassie Configuration

Bio-Safe Coomassie stain is available in a 1 L bottle or a 5 L cube.

Bio-Safe Coomassie

Eliminate the use of hazardous chemicals.

2-D after Bio-Safe Coomassie Stain

2-D gel stained with Bio-Safe Coomassie stain.First dimension: 300 µg of E. coli lysate treated with RNase and DNase in rehydration buffer containing 0.1% Bio-Lyte ampholytes was loaded onto a 17 cm ReadyStrip IPG strip (pH 4–7) and focused 92,000 V-hr on the PROTEAN IEF cell. Second dimension: The strip was loaded onto a 20 x 20.5 cm PROTEAN Plus 8–16% gel, and run at 200 V constant for 6 hr.

Coomassie blue dyes are a family of dyes commonly used to stain proteins in SDS-PAGE gels. The gels are soaked in dye, and excess stain is then eluted with a solvent ("destaining"). This treatment allows the visualization of proteins as blue bands on a clear background. Bio-Rad offers Coomassie stains in four formats.

QC Colloidal Coomassie (161-0803) — the newest in the family of Bio-Rad Coomassie stains, QC Colloidal Coomassie G-250 allows for flexible staining and destaining times and does not require use of methanol for fixing.

  • Low background, high sensitivity, superior reproducibility
  • Environmentally friendly formulation — no addition of methanol or acetic acid required; eliminates the need for hazardous waste disposal
  • Flexible staining and destaining times — from 1 hour to overnight
  • No alcohol addition or dilution steps when staining polyacrylamide gels
  • One-part, ready-to-use colloidal Coomassie stain

Bio-Safe™ Coomassie (161-0786) — Bio-Safe Coomassie Brilliant Blue G-250 stain is fast, simple, sensitive, and convenient.

  • Premixed, ready-to-use, nonhazardous solution
  • No methanol or acetic acid required for destaining
  • Bio-Safe composition reduces solvent waste disposal costs

Coomassie Brilliant Blue R-250 (161-0436) — Coomassie Brilliant Blue R-250 staining solution is the fastest and easiest way to Coomassie-stain Criterion™ precast gels or other polyacrylamide protein gels.

Coomassie Reagents — Coomassie Brilliant Blue R-250 and G-250 powders are available in 10 gram vials.

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QC Colloidal Coomassie Stain
QC Colloidal Coomassie Stain

1610803
1 L, ready-to-use, non-hazardous colloidal Coomassie G-250 stain for protein polyacrylamide gels

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$84.00
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Bio-Safe Coomassie Stain

1610786
1 L, premixed staining solution, for polyacrylamide protein gels

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$78.00
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Bio-Safe Coomassie Stain

1610787
5 L, premixed staining solution, for polyacrylamide protein gels

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$300.00
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Coomassie Brilliant Blue R-250 Staining Solutions Kit

1610435
Kit for staining protein-containing polyacrylamide gels, includes 1 L Coomassie Brilliant Blue R-250 staining solution and 2 x 1 L destaining solution

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$130.00
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Coomassie Brilliant Blue R-250 Staining Solution

1610436
1 L, Coomassie Brilliant Blue R-250 staining solution

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$51.00
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Coomassie Brilliant Blue R-250 Staining Solution

1610437
4 x 1 L, Coomassie Brilliant Blue R-250 staining solution

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$205.00
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Coomassie Brilliant Blue R-250 Destaining Solution

1610438
1 L, Coomassie Brilliant Blue R-250 destaining solution

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$38.00
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Coomassie Brilliant Blue R-250 Destaining Solution

1610439
4 x 1 L, Coomassie Brilliant Blue R-250 destaining solution

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$155.00
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Coomassie Brilliant Blue R-250

1610400
10 g, Coomassie Brilliant Blue R-250 protein stain powder

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$20.00
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Coomassie Brilliant Blue G-250

1610406
10 g, Coomassie Brilliant Blue G-250 protein stain powder

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$35.00
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Number Description Options
2423 Bio-Safe Coomassie Stain Brochure, Rev D Click to download
4307051 Instructions for Staining Polyacrylamide Gels, Rev B Click to download
10032602 QC Colloidal Coomassie Stain Manual, Rev A Click to download