Comparative Proteomics Kit II: Western Blot Module

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Comparative Proteomics Kit II:  Western Blot Module

Overview

Lab Preparation Checklist
Kit contains sufficient materials for 8 student workstations. (2–4 students per workstation)
Primary antibody (anti-myosin light chain mouse monoclonal), lyophilized 1
Secondary antibody (goat anti-mouse polyclonal antibody conjugated to
horseradish peroxidase (HRP), lyophilized
1
HRP color detection reagent A
1
HRP color detection reagent B 1
10x Tris-glycine, 1 L 1
Non-fat dry milk blocker 1
10x Phosphate buffered saline (PBS), 100 ml 2
10% Tween-20, 5 ml 1
Nitrocellulose, 0.45 μm 8 sheets
Blotting paper 16 sheets
Reagent tubes 25
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Required Accessories Not Included in Kit:  
Comparative Proteomics Kit I: Protein Profiler Module 1
Ready Gel precast polyacrylamide gels, 15%, 10-well each 8
Vertical gel electrophoresis chambers 4
Adjustable micropipet, 2–20 µl 4–8
Power supplies 2–4
Water bath 1
Rocking platform 1
Reagent alcohol or ethanol, 0.8–2 L  
Fish samples 5–8 types
Recommended (Optional) Accessories:  
Electroblotter 4
Western Blot Flowchart
Western Blot Flowchart
Key Kit Features
  • Explore immunodetection
  • Explain HIV detection
  • Apply immunology
  • Antibodies as tools
  • Complete in four 45 minute lab sessions
Curriculum Fit
Comparative Proteomics Kit II Curriculum Fit
Western Blotting and Antibodies

Western blotting employs antibodies to pinpoint specific proteins of interest in complex protein mixtures such as cell extracts. Because of its accuracy, western blotting is used as the confirmatory diagnostic test for HIV and mad cow disease (bovine spongiform encephalopathy). Western blotting is used extensively in research to determine the presence of specific proteins, to quantify their expression levels, and to determine whether they have undergone genetic or posttranslational modifications. This surefire method categorically identifies proteins of interest based on two distinguishing features: molecular mass and antibody binding specificity.

When foreign invaders are detected, animal immune systems naturally generate antibodies and tag them for destruction. The ability of antibodies to act like magic bullets and target viral, bacterial, and allergenic antigens in the body also makes them ideal tools in the hunt for specific molecules in bioscience research and diagnostic tests.

Western blot of protein profiler gel reveals precise location of myosin light chain proteins.

PowerPoint Presentation for Workshops and for Teaching

Comparative Proteomics Kit II: Western Blot Module

This PowerPoint presentation is intended for educational use only. The presentation provides educators with background on the Comparative Proteomics Kit II: Western Blot Module and includes instructions for using the kit.

Comparative Proteomics Kit II: Western Blot Module PowerPoint presentation
Comparative Proteomics Kit II: Western Blot Module
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The Comparative Proteomics Kit II: Western Blot Module is the second part of a two-part laboratory. In the first part of this laboratory (Comparative Proteomics Kit I: Protein Profiler Module), students use sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to generate protein profiles and to visualize the unique array of proteins comprising muscle tissues from different fish. In this second part of the laboratory, students apply western blotting techniques to their polyacrylamide gel results to specifically identify myosin light chain from the hundreds of proteins that comprise the muscle cell extracts of closely and distantly related species of fish.

Features and Benefits

  • Applies immunology
  • Teaches students to use antibodies as detection tools
  • Serves as a laboratory extension for the Comparative Proteomics I Kit: Protein Profiler Module

How It Works

The protocol involves transferring the protein bands from polyacrylamide gels to a membrane and then using an anti-myosin light chain antibody to precisely identify which protein in each species' profile is myosin light chain. Using Internet-based bioinformatics databases, students can compare their experimentally determined results to actual protein sequence data derived from DNA and RNA sequences and consider whether variations in myosin among species are due to "genetic" or "epigenetic" factors.

More Information

  • Four-session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)  
Primary antibody (anti-myosin light chain mouse monoclonal), lyophilized 1
Secondary antibody (goat anti-mouse polyclonal antibody conjugated to horseradish peroxidase (HRP), lyophilized 1
HRP color detection reagent A 1
HRP color detection reagent B 1
10x Tris-glycine 1 L
Non-fat dry milk blocker 1
10x phosphate buffered saline (PBS) 2 x 100 ml
10% Tween 20 5 ml
Nitrocellulose sheets, 0.45 µm 8
Blotting paper sheets 16
Reagent tubes 25
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
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Comparative Proteomics Kit II: Western Blot Module

1662800EDU
Western blot classroom study kit, includes antibodies, color detection reagents, 10x TG buffer, 10x PBS, 10% Tween, nitrocellulose, curriculum, and more, for 32 students; education use only

0 1662800EDU /templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-II-/166-2800EDU_0921121043270.xml 1662800EDU <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jd0nawbijd0nawbj" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jd0nawbijd0nawbj"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-II-/166-2800EDU_0921121043270.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:10 PM true true internet/product_SKU LSE N en 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:24 1662800edu-comparative-proteomics-kit-ii-western-blot-module 1662800EDU Comparative Proteomics Kit II: Western Blot Module Comparative Proteomics Kit II: Western Blot Module Western blot classroom study kit, includes antibodies, color detection reagents, 10x TG buffer, 10x PBS, 10% Tween, nitrocellulose, curriculum, and more, for 32 students; education use only Western blot classroom study kit, includes antibodies, color detection reagents, 10x TG buffer, 10x PBS, 10% Tween, nitrocellulose, curriculum, and more, for 32 students; education use only Western blot classroom study kit, includes antibodies, color detection reagents, 10x TG buffer, 10x PBS, 10% Tween, nitrocellulose, curriculum, and more, for 32 students; education use only /webroot/web/images/lse/products/protein_analysis_kits/sku_thumb/global/166-2800EDU_tn.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_view/global/166-2800EDU_view.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_feature/global/166-2800EDU_feature.jpg RepEx 166-2800EDU Western blot classroom study kit, includes antibodies, color detection reagents, 10x TG buffer, 10x PBS, 10% Tween, nitrocellulose, curriculum, and more, for 32 students; education use only 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-II- N 0 1662800, 1662800EDU, comparative, proteomics, ii, western, blot, module, westerns, blots, blotting, detection, immunodetection, science, education, products, classroom false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662800EDU&CartQuote=QT&quantity= 1711 true true false $248.75 true %24199.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662800EDU&AddToDefaultWishList=true&quantity= true false true 41105300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662800EDU&quantity= 1662800EDU
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Comparative Proteomics Kits I and II: Protein Profiler Module and Western Blot Module

1662850EDU
Comprehensive protein classroom study kit, includes Protein Profiler Module (1662700EDU) and Western Blot Module (1662800EDU), for 32 students; education use only

1 1662850EDU /templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-2850EDU_0921121041828.xml 1662850EDU 09/21/09 12:10 PM N en 09/21/19 12:10 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSE 2017-11-14 07:07:24 1662850edu-comparative-proteomics-kits-i-ii-protein-profiler-module-western-blot-module 1662850EDU Comparative Proteomics Kits I and II: Protein Profiler Module and Western Blot Module Comparative Proteomics Kits I and II: Protein Profiler Module and Western Blot Module Comprehensive protein classroom study kit, includes Protein Profiler Module (1662700EDU) and Western Blot Module (1662800EDU), for 32 students; education use only Comprehensive protein classroom study kit, includes Protein Profiler module (#166-2700EDU) and Western Blot module (#166-2800EDU), for 32 students; education use only Comprehensive protein classroom study kit, includes Protein Profiler Module (1662700EDU) and Western Blot Module (1662800EDU), for 32 students; education use only /webroot/web/images/lse/products/protein_analysis_kits/sku_thumb/global/166-2850EDU_tn.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_view/global/166-2850EDU_view.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_feature/global/166-2850EDU_feature.jpg RepEx 166-2850EDU Comprehensive protein classroom study kit, includes Protein Profiler module (#166-2700EDU) and Western Blot module (#166-2800EDU), for 32 students; education use only 1662850, 1662850EDU, comparative, proteomics, and, ii, protein, profiler, module, western, blot, analysis, structure, function, sds, page, separation, westerns, blots, blotting, immunodetection, science, education, products, classroom 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I-- N 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662850EDU&CartQuote=QT&quantity= 1711 true true false $462.50 true %24370.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662850EDU&AddToDefaultWishList=true&quantity= true false true 41105300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662850EDU&quantity= 1662850EDU
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Rapid Blotting and V3 Western Workflow™ Starter Kit

1662875EDU
Comprehensive protein classroom study kit, includes Protein Profiler Module (1662700EDU), Western Blot Module (1662800EDU), Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs (10), 4–20% Mini-PROTEAN® TGX Stain-Free™ Gels (10), application note, for 32 students; education use only

1662875EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Imaging/166-2875EDU_1344282022.xml 1662875EDU <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jd0nawbijd0nawbj" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jd0nawbijd0nawbj"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Imaging/166-2875EDU_1344282022.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Node> </Deployments> 08/06/12 09:55 AM true true internet/product_SKU LSE N en 03/06/22 12:39 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:19 1662875edu-rapid-blotting-v3-western-workflow-starter-kit 1662875EDU Rapid Blotting and V3 Western Workflow Starter Kit Rapid Blotting and V3 Western Workflow&#153; Starter Kit Comprehensive protein classroom study kit, includes Protein Profiler Module (1662700EDU), Western Blot Module (1662800EDU), Trans-Blot<sup>&#174;</sup> Turbo&#153; Mini Nitrocellulose Transfer Packs (10), 4&ndash;20&#37; Mini-PROTEAN<sup>&#174;</sup> TGX Stain-Free&#153; Gels (10), application note, for 32 students; education use only Comprehensive protein classroom study kit, includes Protein Profiler Module (1662700EDU), Western Blot Module (1662800EDU), Trans-Blot<sup>&#174;</sup> Turbo&#153; Mini Nitrocellulose Transfer Packs (10), 4&ndash;20&#37; Mini-PROTEAN<sup>&#174;</sup> TGX Stain-Free&#153; Gels (10), application note, for 32 students; education use only /webroot/web/images/lse/products/protein_analysis_kits/sku_thumb/global/166-2875EDU_tn.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_view/global/166-2875EDU_view.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_feature/global/166-2875EDU_feature.jpg Molly Gregg 166-2875EDU Comprehensive protein classroom study kit, includes Protein Profiler Module (#166-2700EDU), Western Blot Module (#166-2800EDU), Trans-Blot<sup>&#174;</sup> Turbo&#153; mini nitrocellulose transfer packs (10), 4&ndash;20&#37; Mini-PROTEAN<sup>&#174;</sup> TGX Stain-Free&#153; gels (10), application note, for 32 students; education use only Comprehensive protein classroom study kit, includes protein profiler module (#166-2700EDU), western blot module (#166-2800EDU), Trans-Blot<sup>&#174;</sup> Turbo&trade; mini nitrocellulose transfer pack, TGX Stain-Free&#153; gels, application note, for 32 students; education use only 08/06/12 09:55 AM 03/06/22 12:39 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Equipment-and-Supplies/Imaging N 0 <p><strong>Western blotting in less than 2 hours!</strong> Using our new rapid blotting or V3 Western Workflow (stain-free rapid blotting) option allows you to complete the entire western blot workflow in less than 2&ndash;5 hours, depending on which time-saving steps you incorporate. Mini-PROTEAN TGX Stain-Free gels (4&ndash;20%) combined with our superfast Trans-Blot Turbo transfer system provide the fastest speed and most time savings, allowing you to complete the workflow in less than a single 3-hr lab block. Teach your students about the exciting new chemistry that allows visualization of samples separated on PAGE gels without staining! To learn more, download the <a href="/webroot/web/pdf/lse/literature/M1662876EDU.pdf" target="_blank"> Rapid Blotting + V3 Western Workflow<sup>&trade;</sup> Application Note.</a></p> /webroot/web/html/lse/products/SKUs/autopopulated 166-2875EDU_1344282022_XX_en.xml /webroot/web/html/lse/products/SKUs/autopopulated/166-2875EDU_1344282022_XX_en.xml Kit contents 2807 Sufficient materials for 8 student workstations with 2–4 students per workstation, plus additional reagents for further studies 1 0 Kit I 2812 Comparative Proteomics Kit I: Protein Profiler Module, 1 1 0 Kit II 2813 Comparative Proteomics Kit II: Western Blot Module, 1 1 0 Transfer packs 2815 Trans-Blot Turbo mini nitrocellulose transfer packs, 1 pkg of 10 1 0 Gels 2811 4&ndash;20% Mini-PROTEAN TGX Stain-Free gels, 1 pkg of 10 1 0 Application note 2810 Rapid Blotting + V3 Western Workflow<sup>&trade;</sup> Application Note, 1 1 0 Notes 2814 Obtain fish samples locally. Mini-PROTEAN TGX Stain-Free gels require a UV imager to visualize the resolved protein samples. Traditional SDS-PAGE gels can be used in place of Mini-PROTEAN TGX Stain-Free gels but require staining/destaining of gels to visualize the resolved protein samples. 1 0 false /en-us/education/promotions/free-starter-kit-with-purchase-trans-blot-turbo false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662875EDU&CartQuote=QT&quantity= 1711 true true false $717.50 true %24574.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662875EDU&AddToDefaultWishList=true&quantity= true false true 41105300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662875EDU&quantity= 1662875EDU
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Western Blot Temperature Sensitive Reagents Bag

1662801EDU
Consumables refill reagent kit for use with Comparative Proteomics Kit II: Western Blot Module (1662800EDU), includes antibodies and color detection reagents; education use only

1662801EDU /templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-II-/166-2801EDU_0921121043215.xml 5 1662801EDU 09/21/09 12:10 PM N en 09/21/19 12:10 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSE 2017-11-14 07:07:24 1662801edu-western-blot-temperature-sensitive-reagents-bag 1662801EDU Western Blot Temperature Sensitive Reagents Bag Western Blot Temperature Sensitive Reagents Bag Consumables refill reagent kit for use with Comparative Proteomics Kit II: Western Blot Module (1662800EDU), includes antibodies and color detection reagents; education use only Consumables refill reagent kit for use with Comparative Proteomics Kit II: Western Blot Module (#166-2800EDU), contains antibodies and color detection reagents; education use only Consumables refill reagent kit for use with Comparative Proteomics Kit II: Western Blot Module (1662800EDU), includes antibodies and color detection reagents; education use only /webroot/web/images/lse/products/protein_analysis_kits/sku_thumb/global/166-2801EDU_tn.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_view/global/166-2801EDU_view.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_feature/global/166-2801EDU_feature.jpg RepEx 166-2801EDU Consumables refill reagent kit for use with Comparative Proteomics Kit II: Western Blot Module (#166-2800EDU), contains antibodies and color detection reagents; education use only 1662801, 1662801EDU, western, blot, temperature, sensitive, reagents, bag, refill, comparative, proteomics, II, module, antibodies, color, detection, solutions, science, education, products, classroom 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-II- N 0 <p>Western Blot Temperature-Sensitive Reagents Bag contains consumable refill reagents for use with Comparative Proteomics Kit II: Western Blot Module (#1662800EDU). The bag contains antibodies and color detection reagents. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated 166-2801EDU_0921121043215_en.html /webroot/web/html/lse/products/SKUs/autopopulated/166-2801EDU_0921121043215_en.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662801EDU&CartQuote=QT&quantity= 1711 false true false $165.00 true %24132.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662801EDU&AddToDefaultWishList=true&quantity= true false true 41105300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662801EDU&quantity= 1662801EDU
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Primary Antibody (anti-myosin light chain antibody)

1662804EDU
200 μg, lyophilized, primary antibody (anti-myosin light chain antibody); education use only

1662804EDU /templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-II-/166-2804EDU_0921121043166.xml 4 1662804EDU <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jd0nawbijd0nawbj" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jd0nawbijd0nawbj"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-II-/166-2804EDU_0921121043166.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:10 PM true true internet/product_SKU LSE N en 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:24 1662804edu-primary-antibody-anti-myosin-light-chain-antibody 1662804EDU Primary antibody (anti-myosin light chain antibody) Primary Antibody (anti-myosin light chain antibody) 200 μg, lyophilized, primary antibody (anti-myosin light chain antibody); education use only 200 μg, lyophilized, primary antibody (anti-myosin light chain antibody); education use only 200 μg, lyophilized, primary antibody (anti-myosin light chain antibody); education use only /webroot/web/images/lse/products/protein_analysis_kits/sku_thumb/global/166-2804EDU_tn.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_view/global/166-2804EDU_view.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_feature/global/166-2804EDU_feature.jpg RepEx 166-2804EDU 200 μg, lyophilized, primary antibody (anti-myosin light chain antibody); education use only Primary antibody, anti-myosin light chain antibody, Comparative Proteomics, Western Blot Module, Western Blot Temperature Sensitive Reagents Bag, classroom refill kit 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-II- N 0 <p>Primary Antibody (anti-myosin light chain antibody), 200 &micro;g, is a lyophilized primary antibody for use with Comparative Proteomics Kit II: Western Blot Module (#1662800EDU).</p> <p>Primary Antibody (anti-myosin light chain antibody) is available as part of the Western Blot Temperature-Sensitive Reagents Bag (#1662801EDU). This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated 166-2804EDU_0921121043166_en.html /webroot/web/html/lse/products/SKUs/autopopulated/166-2804EDU_0921121043166_en.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662804EDU&CartQuote=QT&quantity= 1711 false true false $72.50 true %2458.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662804EDU&AddToDefaultWishList=true&quantity= true false true 41105300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662804EDU&quantity= 1662804EDU
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Goat X Mouse-HRP

1721011EDU
2 ml, EIA-grade, enzyme-antibody conjugate; education use only

1721011EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Quantitation/172-1011EDU_0921121038751.xml 3 1721011EDU <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jd0nawbijd0nawbj" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jd0nawbijd0nawbj"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Quantitation/172-1011EDU_0921121038751.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:10 PM true true internet/product_SKU LSE N en 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:21 1721011edu-goat-x-mouse-hrp 1721011EDU Goat X Mouse-HRP Goat X Mouse-HRP 2 ml, EIA-grade, enzyme-antibody conjugate; education use only 2 ml, EIA-grade, enzyme-antibody conjugate; education use only 2 ml, EIA-grade, enzyme-antibody conjugate; education use only /webroot/web/images/lse/products/equipment_supplies/sku_thumb/global/172-1011EDU_tn.jpg /webroot/web/images/lse/products/equipment_supplies/sku_view/global/172-1011EDU_view.jpg /webroot/web/images/lse/products/equipment_supplies/sku_feature/global/172-1011EDU_feature.jpg RepEx 172-1011EDU 2 ml, EIA-grade, enzyme-antibody conjugate; education use only 1721011, 1721011EDU, goat, x, mouse-hrp, anti-mouse, igg, h + l-hrp, conjugate, anti, mouse, h + l, heavy, light, chain, hrp, hp, horse, radish, horseradish, peroxidase, western, blot, blots, blotting, immune, detection, immunodetection, immunblot, immun-blot, immunoblot, westerns, eia-grade, enzyme, immunoassay, grade, elisa, science, education, products, classroom 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Equipment-and-Supplies/Quantitation N 0 <p>Secondary Antibody (goat anti-mouse HRP), 2 ml, is a lyophilized secondary polyclonal antibody conjugated to horseradish peroxidase; this EIA-grade antibody is a refill used with with Comparative Proteomics Kit II: Western Blot Module (#1662800EDU).</p> <p>Secondary Antibody (goat anti-mouse HRP) is available as part of the Western Blot Temperature-Sensitive Reagents Bag (#1662801EDU). This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated 172-1011EDU_0921121038751_en.html /webroot/web/html/lse/products/SKUs/autopopulated/172-1011EDU_0921121038751_en.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721011EDU&CartQuote=QT&quantity= 1717 false true false $175.00 true %24140.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721011EDU&AddToDefaultWishList=true&quantity= true false true 41105300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1721011EDU&quantity= 1721011EDU
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HRP Conjugate Substrate Kit

1706431EDU
Colorimetric detection for western blotting kit, includes premixed 4CN, hydrogen peroxide solutions, color development buffer; makes 1 L color development solution; education use only

1706431EDU /templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-II-/170-6431EDU_0921121043186.xml 2 1706431EDU 09/21/09 12:10 PM N en 09/21/19 12:10 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSE 2017-11-14 07:07:24 1706431edu-hrp-conjugate-substrate-kit 1706431EDU HRP Conjugate Substrate Kit HRP Conjugate Substrate Kit Colorimetric detection for western blotting kit, includes premixed 4CN, hydrogen peroxide solutions, color development buffer; makes 1 L color development solution; education use only Colorimetric detection for western blotting kit, includes premixed 4CN, hydrogen peroxide solutions, color development buffer; makes 1 L color development solution; education use only Colorimetric detection for western blotting kit, includes premixed 4CN, hydrogen peroxide solutions, color development buffer; makes 1 L color development solution; education use only /webroot/web/images/lse/products/protein_analysis_kits/sku_thumb/global/170-6431EDU_tn.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_view/global/170-6431EDU_view.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_feature/global/170-6431EDU_feature.jpg RepEx 170-6431EDU Colorimetric detection for western blotting kit, includes premixed 4CN, hydrogen peroxide solutions, color development buffer; makes 1 L color development solution; education use only 1706431, 1706431EDU, hrp, substrate, conjugate, 4cn, horseradish, peroxidase, colorimetric, western, blot, blots, blotting, detection, immunodetection, science, education, products, classroom 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-II- N 0 <p>HRP Conjugate Substrate Kit is a colorimetric detection kit for western blotting. It includes premixed 4CN, hydrogen peroxide solutions, and color development buffer. The kit makes 1 L color development solution and can be used as a refill kit for the Comparative Proteomics Kit II: Western Blot Module (#1662800EDU). This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated 170-6431EDU_0921121043186_en.html /webroot/web/html/lse/products/SKUs/autopopulated/170-6431EDU_0921121043186_en.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706431EDU&CartQuote=QT&quantity= 1717 false true false $118.00 true %2494.40 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706431EDU&AddToDefaultWishList=true&quantity= true false true 41116100 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1706431EDU&quantity= 1706431EDU
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Nitrocellulose/Filter Paper Pack

1662807EDU
0.45 µm, 7 x 8.4 cm, nitrocellulose membrane (8 sheets)/filter paper (16 sheets) sandwich; education use only

1662807EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Reagents/166-2807EDU_0921121022883.xml 1 1662807EDU 09/21/09 12:10 PM N en 09/21/19 12:10 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSE 2017-11-14 07:07:22 1662807edu-nitrocellulose-filter-paper-pack 1662807EDU Nitrocellulose/Filter Paper Pack Nitrocellulose/Filter Paper Pack 0.45 &micro;m, 7 x 8.4 cm, nitrocellulose membrane (8 sheets)/filter paper (16 sheets) sandwich; education use only 0.45 μm, 7 x 8.4 cm, nitrocellulose membrane (8 sheets) /filter paper (16 sheets) sandwich; education use only 0.45 &micro;m, 7 x 8.4 cm, nitrocellulose membrane (8 sheets)/filter paper (16 sheets) sandwich; education use only /webroot/web/images/lse/products/equipment_supplies/sku_thumb/global/166-2807EDU_tn.jpg /webroot/web/images/lse/products/equipment_supplies/sku_view/global/166-2807EDU_view.jpg /webroot/web/images/lse/products/equipment_supplies/sku_feature/global/166-2807EDU_feature.jpg RepEx 166-2807EDU 0.45 μm, 7 x 8.4 cm, nitrocellulose membrane (8 sheets) /filter paper (16 sheets) sandwich; education use only 1662807, 1662807EDU, nitrocellulose, filter, paper, pack, refill, cloning, sequencing, explorer, series, western, blotting, blot, transfer, 0.45um, 7x8.4 science, education, products 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Equipment-and-Supplies/Reagents N 0 <p>The Nitrocellulose/Filter Paper Pack contains eight nitrocellulose/filter paper sandwiches: 0.45 &micro;m, 7 x 8.4 cm nitrocellulose membrane sandwiched between two sheets of filter paper. This product can be used as a refill for the Comparative Proteomics Kit II: Western Blot Module (#1662800EDU) and for classroom laboratory blotting needs. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated 166-2807EDU_0921121022883_en.html /webroot/web/html/lse/products/SKUs/autopopulated/166-2807EDU_0921121022883_en.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662807EDU&CartQuote=QT&quantity= 1717 true true false $72.50 true %2458.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662807EDU&AddToDefaultWishList=true&quantity= true false true 41105300 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662807EDU&quantity= 1662807EDU
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Hazardous  
Rapid Blotting + V3 Western Workflow Application Note

1662876EDU
Application note for western blotting, describes rapid blotting using the Trans-Blot® Turbo Transfer System and V3 Western Workflow (stain-free blotting) methods; education use only Use Add to Cart to order a printed copy or download a free PDF.

1662876EDU /templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-2876EDU_1353534279.xml 1662876EDU <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jd0nawbijd0nawbj" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jd0nawbijd0nawbj"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I--/166-2876EDU_1353534279.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Node> </Deployments> 11/21/12 01:33 PM true true internet/product_SKU LSE N en 11/21/22 01:39 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:24 1662876edu-rapid-blotting-v3-western-workflow-application-note 1662876EDU Rapid Blotting+V3 Western Workflow Application Note Rapid Blotting + V3 Western Workflow<sup>&trade;</sup> Application Note Application note for western blotting, describes rapid blotting using the Trans-Blot<sup>&reg;</sup> Turbo<sup>&trade;</sup> Transfer System and V3 Western Workflow (stain-free blotting) methods; education use only Use Add to Cart to order a printed copy or <a href="/webroot/web/pdf/lse/literature/M1662876EDU.pdf" target="_blank">download a free PDF</a>. Application note for western blotting, describes rapid blotting using the Trans-Blot<sup>&reg;</sup> Turbo<sup>&trade;</sup> Transfer System and V3 Western Workflow (stain-free blotting) methods; education use only Use Add to Cart to order a printed copy or <a href="//webroot/web/pdf/lse/literature/M1662876EDU.pdf" target="_blank">download a free PDF</a>. /webroot/web/images/lse/products/protein_analysis_kits/sku_thumb/global/166-2876EDU_tn.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_view/global/166-2876EDU_view.jpg /webroot/web/images/lse/products/protein_analysis_kits/sku_feature/global/166-2876EDU_feature.jpg Caryn Eaton Rapid Blotting + V3 Western Workflow<sup>&trade;></sup> Application Note Application note for western blotting, describes rapid blotting using the Trans-Blot<sup>&reg;</sup> Turbo<sup>&trade;</sup> transfer system and V3 Western Workflow (stain-free blotting) methods; education use only rapid blotting, v3 western, workflow 11/21/12 01:33 PM 11/21/22 01:39 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Protein-Analysis-Kits/Comparative-Proteomics-Kit-I-- N 0 <p><strong>Western blotting in less than 2 hours!</strong> This application note describes two methods that allow you to complete the entire western blot workflow in less than 2&ndash;5 hours, depending on which time-saving steps you incorporate.</p> <ul> <li>Rapid blotting using traditional SDS-PAGE technology coupled with the Trans-Blot<sup>&reg;</sup> Turbo<sup>&trade;</sup> transfer system</li> <li>V3 Western Workflow (stain-free blotting), which incorporates stain-free technology into the rapid blotting protocol </li> </ul> /webroot/web/html/lse/products/SKUs/autopopulated 166-2876EDU_1353534279_XX_en.xml /webroot/web/html/lse/products/SKUs/autopopulated/166-2876EDU_1353534279_XX_en.xml 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662876EDU&CartQuote=QT&quantity= 1711 false true false $18.75 true %2415.00 /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662876EDU&AddToDefaultWishList=true&quantity= true false true 55101500 true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1662876EDU&quantity= 1662876EDU
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Number Description Options
M1662876EDU Bio-Rad Explorer™ Rapid Blotting + V3 Western Workflow (Stain-Free Rapid Blotting) Application Note, Rev A Click to download
10004531 Instruction Manual, Comparative Proteomics Kit II: Western Blot Module, Bio-Rad Explorer Rev E Please login to download this document
5478 Instruction Manual, Comparative Proteomics Kit II: Western Blot Module Quick Guide, Bio-Rad Explorer™, Rev A Click to download
6933 Fast Electrophoresis and Western Blotting Flier Click to download

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