Clarity™ and Clarity Max™ Western ECL Blotting Substrates

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Clarity Western ECL Substrate

Overview

Chemiluminescence Chemistry

Chemiluminescence detection. The secondary antibody, which binds the antibody specific for the protein of interest, is conjugated to an enzyme; when it acts on the substrate, light is emitted.

Clarity Western ECL Substrate Performance

 Clarity Comparison

Comparison of chemiluminescent substrate sensitivity. A, five different western blots were loaded with the same protein loads of purified alcohol dehydrogenase (ADH). Blots were incubated with substrates per manufacturer recommendations, then imaged for 10 sec on the ChemiDoc™ MP Imaging System. B, the signal-to-noise ratio for each band is depicted in bar graph form. Clarity and ECL Plus provided the best signal-to-noise ratios across all protein loads.

Clarity Max Western ECL Substrate Performance

 Clarity Comparison

Comparison of chemiluminescent substrate signal intensity. A, five different western blots were loaded with the same protein loads of purified IKB alpha. Blots were incubated with substrates per manufacturer recommendations, then imaged for 70 sec on the ChemiDoc Touch Imaging System. B, the signal-to-noise ratios for the bands in the boxed area for Clarity Max, ECL Select, and SuperSignal West Femto are depicted in bar graph form. Clarity Max and ECL Select provided the best signal-to-noise ratios across all protein loads. ND, not determined; signal at 30 pg for SuperSignal West Femto was not detectable above background noise.



Clarity Family of Western ECL Blotting Substrates

Chemiluminescent western blot detection is a highly sensitive alternative to isotopic detection of proteins bound to blotting membranes. Bio-Rad’s Clarity family of western ECL substrates provides simple, high-performance solutions for all your western chemiluminescence needs.

Clarity ECL Substrate is our most versatile chemiluminescence substrate, while Clarity Max ECL Substrate is the substrate of choice for challenging blots that require higher sensitivity. Both substrates are compatible with any HRP conjugate and ideal for both digital and film-based imaging. Substrate components are stable at room temperature.

Clarity Western ECL Substrate

  • Mid-femtogram-level sensitivity
  • 24 hour signal duration
  • 1 year shelf life at room temperature
  • Ideal for digital and film-based imaging

Clarity ECL Substrate exhibits excellent visual limit of detection versus other commonly used ECL substrates on the market.


 

Clarity Max Western ECL Substrate

  • Low-femtogram-level sensitivity
  • Recommended for detection of low target amounts
  • Ideal for digital and film-based imaging

Clarity Max ECL Substrate exhibits superior visual limit of detection versus other commonly used high-performance ECL substrates on the market.


 

Packaging Options

ECL Substrate Number of applications Total Volume Catalog Number
Clarity Western ECL Substrate  16 to 28 membranes 200 ml 1705060
32 to 56 membranes 500 ml 1705061
Clarity Max Western ECL Substrate 7 to 12 membranes 100 ml 1705062
Lower detection limit Clarity: Mid-femtogram level
Clarity Max: Low-femtogram level
Signal duration Clarity: 24 hr
Clarity Max: 2 hr
Primary detection method Digital or film imaging
Suggested antibody dilution Primary: 1:1,000–1:50,000;
Secondary: 1:50,000–1:250,000
Recommended membrane Nitrocellulose, PVDF, or LF PVDF
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170-5060 image
Clarity™ Western ECL Substrate, 200 ml

1705060
Contains 100 ml Clarity Western Peroxide Reagent and 100 ml Clarity Western Luminol/Enhancer reagent

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$135.00
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Clarity Western ECL Substrate, 500 ml
Clarity™ Western ECL Substrate, 500 ml

1705061
Contains 250 ml Clarity Western Peroxide Reagent and 250 ml Clarity Western Luminol/Enhancer Reagent

List Price:
 
$255.00
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Clarity Max™ Western ECL Substrate, 100 ml

1705062
Pkg of 1, contains 50 ml Clarity Max Western Peroxide Reagent and 50 ml Clarity Max Luminol/Enhancer Reagent.

List Price:
 
$300.00
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Number Description Options
6305 Clarity™ and Clarity Max™ Western ECL Substrates Brochure, Ver B Click to download [ Add to Cart (Free) ]
10026691 Clarity Western ECL Substrate Transition Guide, Rev A Click to download
10026527 Clarity™ and Clarity Max™ Western ECL Substrates Quick Start Guide, Ver D Click to download
10026389 Instruction Manual, Clarity™ and Clarity Max™ Western ECL Substrates, Ver C (D085075) Click to download
6148 Transfer of High Molecular Weight Proteins to Membranes: A Comparison of Transfer Efficiency Between Blotting Systems, Rev B Click to download