ChemiDoc™ MP Imaging System

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ChemiDoc MP System

Overview

V3 Western Workflow™
Fluorophore Compatibility Chart

Fluorophores
      Starbright™  
DyLight 488 DyLight 550 DyLight 650 DyLight 680 DyLight 800
Alexa Fluor 488 Alexa Fluor 546 Alexa Fluor 647 Alexa--a Fluor 680 Alexa Fluor 790
Cy2 Cy3 Cy5 Cy5.5 Cy7
  Rhodamine SYPRO Ruby IRDye 680 IRDye 800
Imaging System Selection Guide

  ChemiDoc MP ChemiDoc Touch Gel Doc XR+ GelDoc EZ GS-900
ChemiDoc™
MP
ChemiDoc™
ChemiDoc™
XRS+
Gel Doc™
XR+
Gel Doc™
EZ
GS-900™
Nucleic Acid Detection
Ethidium Bromide
SYBR® Green I and SYBR® Safe Dyes
Fast Blast™ DNA Stain
Protein Staining, 1-D Gels
Stain-Free Gels
Coomassie Blue Stain
Silver Stain
SYPRO Ruby, Flamingo, and Oriole Protein Blot Stain
Protein Staining, 2-D Gels
Coomassie Blue Stain
Silver Stain
SYPRO Ruby, Flamingo, and Oriole Gel Stains
Cy2, Cy3, Cy5 Label
Blot Detection
Multiplex fluorescence
Immun-Star™
Chemiluminescence
Stain-free blots
Colorimetric blots
SYPRO Ruby Protein Blot Stain*

*  Optimal with low fluorescence PVDF membrane.


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ChemiDoc Touch
 
What Is Stain-Free Technology?
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The ChemiDoc MP Imaging System is a full-feature instrument for imaging and analyzing gels and western blots. It is designed to address multiplex fluorescent western blotting, chemiluminescence detection, general gel documentation applications, and stain-free technology imaging needs.

Features and Benefits

  • All-in-one flexible imaging — get precise, reproducible fluorescence, chemiluminescence, and colorimetric gel and blot detection, analysis and documentation in a single system
  • Improve your imaging capabilities with the ChemiDoc MP — benefit from the advantages of using a wider range of fluorescent reporters (RGB, far red, and near infrared) including StarBright™ Fluorophores; see the fluorophore compatibility chart
  • Multiplex fluorescent western blotting — detect up to three proteins simultaneously and eliminate the need for stripping and reprobing
  • Ease of use — features include automatic selection of optimal light source by application, auto focus, auto exposure, and preview features ensure optimal images. Learn in minutes with on screen help
  • Stain-free protein normalization — stain-free imaging permits the normalization of bands to total protein in both in gels and blots; eliminates the need for housekeeping proteins
  • Sample Trays and Smart Tray Technology™ — three sample trays available to cover diverse imaging applications. Smart Tray Technology automatically recognizes the application-specific trays and adjusts imaging parameters and software options accordingly

For western blotting, the ChemiDoc™ MP V3 Western Workflow™ provides the ability to rapidly assess electrophoresis and blot transfer quality prior to blot processing, to perform total protein normalization and multiplexing using a wide rance of fluorophores.

Applications and Uses

  • Electrophoresis — protein, including stain-free, and nucleic acid gel imaging and documentation
  • Blotting — identify and quantify proteins, nucleic acids, protein-ligand interactions, etc. with fluorescence, chemiluminescence, or colorimetric detection

Included Components

  • Internal computer with Image Lab™ Touch Software
  • 12” touch screen display
  • Image Lab Software to install on your computer
  • Blot/UV/Stain-Free Sample Tray for Bio-Rad stain-free gels and blots, fluorescence multiplexing, chemiluminescence detection, ethidium bromide and SYPRO Ruby (12003028)
  • Clarity™ (1705060) and Clarity Max™ (1705062) Western ECL Substrates; Precision Plus Protein™ All Blue Prestained (1610373) and Precision Plus Protein Unstained, Strep-tagged (1610363) Protein Standards

Mini and Midi ChemiDoc MP V3 Western Workflows also include:

  • ChemiDoc MP Imaging System
  • Mini-PROTEAN® Tetra Cell, 4-gel (1658004) or Criterion™ Cell (1656001) Electrophoresis Chamber
  • PowerPac™ Basic Power Supply (1645050)
  • Trans-Blot® Turbo™ Transfer System (1704150)
  • 4–20% Mini-PROTEAN® TGX Stain-Free™ or 4–20% Criterion™ TGX Stain-Free™ Protein Gels
  • Buffers, Nitrocellulose Transfer Packs, Immun-Blot® LF PVDF Membranes, and other consumables

Optional Components

  • White Sample Tray for Coomassie Blue, copper, silver, and zinc stains (12003026)
  • Blue Sample Tray for nucleic acid applications with GelGreen or any SYBR® Stain (12003027)
  • Holder for ChemiDoc Sample Trays and UV Shield (1708377)
  • Gel Alignment Templates for ChemiDoc MP and ChemiDoc Imaging Systems (12003915)
  • UV Safety Shield for ChemiDoc MP and ChemiDoc Imaging Systems (12003914)

Configuration Options

Product Catalog Number
ChemiDoc MP Imaging System 17001402
ChemiDoc MP V3 Western Workflow for Mini Gels 17001395
ChemiDoc MP V3 Western Workflow for Midi Gels 17001389

Note: Also available are the ChemiDoc Imaging System (17001401), ChemiDoc V3 Western Workflows for Mini Gels (17001403), and ChemiDoc V3 Western Workflows for Midi Gels (17001390), which have a more limited fluorescence capability than ChemiDoc MP Systems. These systems can be upgraded to a broader spectrum (full RGB, far red, and near IR) to enable fluorescence multiplexing with the ChemiDoc to ChemiDoc MP Upgrade Kit (12003263).

Related Products

Related Categories

Discontinued Product
Discontinued Product

Discontinued Product
The ChemiDoc MP Imaging System featured on this page replaces our classic model of the ChemiDoc MP Imager, which is being discontinued. However, we will continue to provide support for customers with the discontinued model.

Automation Capabilities  
Smart Tray Technology™ Automatic recognition of application-specific tray and adjustment of imaging parameters and software options
Autofocus Precalibrated focus for any zoom setting or sample height
Auto-exposure Two user-defined modes (rapid or optimal auto-exposure) for all blot and gel imaging applications
Image flat fielding Dynamic; precalibrated and optimized for each application
Hardware  
Touch-screen functionality Multitouch capability (2 points) 12.1" display
Maximum image area (W x H) 21 x 16.8 cm
Detector Cooled CCD, 6 megapixels
Dynamic range >4 orders of magnitude
Illumination sources Trans-UV, 302 nm excitation
Epi-white
Trans-white (requires White Sample Tray)
Trans-blue, 450−490 nm excitation (requires Blue Sample Tray)
Epi-blue, 460−490 nm excitation
Epi-green, 520−545 nm excitation
Epi-red, 625−650 nm excitation
Epi-far red, 650−675 nm excitation
Epi-near IR, 755−777 nm excitation
Filter and application 590/110 nm standard filter to perform protein and DNA gel and blot imaging
Chemiluminescence filter
518–546 nm filter for blue-excitable fluorophores and stains
577–613 nm filter for green-excitable fluorophores and stains
675–725 nm for red-excitable fluorophores and stains
700–730 nm filter for far red-excitable fluorophores and stains
813–860 nm for near IR-excitable fluorophores and stains
Data output 16-bit or 8-bit: SCN, TIFF, JPEG files
Instrument weight 35 kg (78 lb)
Instrument size (L x W x H) 61 x 51 x 53 cm
Operating voltage 100–250 V
Operating temperature 10–28°C
Operating humidity 10–85% relative humidity (non-condensing)
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Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

ChemiDoc™ MP Imaging System

17001402
Gel and blot imaging and analysis system, includes internal computer, 12" touch-screen display, camera, Image Lab™ Touch Software, Image Lab Software, Blot/UV/Stain-Free Sample Tray, Clarity™ and Clarity Max™ Substrates, Precision Plus Protein™ Standards

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ChemiDoc™ MP V3 Western Workflow™ for Mini Gels

17001395
Pkg of 1, complete suite of instruments, accessories, and consumables to run fluorescent or chemiluminescent western blotting experiments from start to finish. Uses precast mini gels. Includes Image Lab™ Software.

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ChemiDoc™ MP V3 Western Workflow™ for Midi Gels

17001389
Pkg of 1, complete suite of instruments and consumables to run fluorescent or chemiluminescent western blotting experiments from start to finish. Uses precast midi gels. Includes Image Lab™ Software.

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Number Description Options
6873 ChemiDoc™ MP Imaging System Brochure, Ver A Click to download
6936 ChemiDoc MP Imaging System Flier, Ver A Click to download
10000062126 ChemiDoc™ and ChemiDoc MP Imaging Systems with Image Lab™ Touch Software User Guide, Ver A Click to download
6427 Bio-Rad® V3 Western Workflow™, Ver C Click to download [ Add to Cart (Free) ]
6493 Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide, Ver C Click to download [ Add to Cart (Free) ]