Chelex® 100 Resin

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Overview

Analytical Grade Resin Selection Guide
Analytical Grade Resin Selection Guide
Change in Structure of Chelex Resin with Increasing pH

Chelex chelating resin is efficiently regenerated in dilute acid and operates in basic, neutral, and weakly acidic solutions of pH 4 or higher. At very low pH, the resin acts as an anion exchanger. This figure shows the zwitterionic forms of the Chelex resin as a function of pH.

Selectivity for Divalent Cations
Hg+2 1060 Fe+2 0.130
Cu+2
126 Mn+2 0.024
UO+2 5.70 Ba+2 0.016
Ni+2 4.40 Ca+2 0.013
Pb+2 3.88 Sr+2 0.013
Zn+2 1.00 Mg+2 0.009
Co+2 0.615 Na+1 0.0000001
Cd+2 0.390    

The selectivity of Chelex resin for metal cations corresponds to that of iminodiacetic acid. This is a list of selectivity factors for several divalent cations. The selectivity factor is a quantitative measure of the affinity that Chelex resin displays for a particular cation compared to its affinity for a reference cation, in this case Zn+2.

Chelex 100 Sample Applications and Resins
Application Reference
Preparation of metal-free apoenzyme O'Keefe, E. T., Hill, R. L. and Bell, J. E., Biochem., 19, 4954 (1980).
Removal of calcium from sarcoplasmic reticulum vesicles Chiesi, M. and Inesi, G., Biochem. 19, 2912 (1980).
Removal of extraneously bound metal ions from enzymes prior to NMR and ESR studies Barker, R., et al., Biochem. J., 177, 289 (1979).
Batch removal of calcium from whole blood Raymond, F A. and Weinshilboum, R. M., Clin. Chim. Acta, 58, 185 (1975).
Removal of metals from ATP Sontheimer, G. M., et al., Biochem., 26, 2701 (1987).
Removal of metals from buffer, brine, and biological solutions Knapp, G., et al., J. Anal. Atomic Spectrometry, 2, 611 (1987); Laue, T. M., et al., Biochem., 28, 4762 (1989).
Removal of metals from S100b and melittin Baudier, J., et al., Biochem., 26, 2886 (1987).
Purification of dinucleotides Reinhardt, C. G. and Krugh, T. R., Biochem., 17, 4845 (1978).
Removal of metals from cell suspension Bosron, W. F., Kennedy F. S. and Vallee, B. L., Biochem., 14, 2275 (1975).
Batch removal of metals from urine Agarwal, M., Bennett, R. B., Stump, I. G. and D'Avria, J. M., Anal. Chem., 47, 924 (1975).
Removal of metals from enzyme solutions Dunn, M. F., Pattison, S. E., Storm, M. C. and Quiel, E., Biochem., 19, 718 (1980).
Removal of metals from guinea pig complement
Amiraian, K., McKinney, J. A. and Duchna, L., Immunology, 26, 1135 (1974).
Removal of aldehydes and peroxides from polyethylene glycol Ray, W. J. and Pavathingal, J. M., Anal. Biochem., 146, 307 (1985).
Removal of interfering components from myo-inositol Brandt, S. J., Dougherty, R. W., Lapetina, E . G. and Niedel, J. E., Proc. Nat. Acad. Sci., 82, 3277 (1985).
Calcium removal from protein kinases Putnam-Evans, C. L., Harmon, A. C. and Cormier, M. J., Biochem., 29, 2488 (1990).
Removal of divalent cations from NMR stock solutions Devlin, C. C. and Grisham, C. M., Biochem., 29, 6192 (1990). Chuknyisky, P. P., Rifkind, J. M., Tarien, E., Beal, R. B. and Eichhorn, G. L., Biochem., 29, 5987 (1990).
Removal of paramagnetic impurities from oligonucleotides for NMR analysis Kochoyan, M., Leroy, J.L. and Guéron, M. Biochem., 29, 4799 (1990), Schroeder, S.A., Roongta, V., Fu J. M., Jones, C.R., and Gorenstein, D.G., Biochem., 28, 8292 (1989).
Trace metal removal from buffer solutions and seawater Pai, S.-C., Chen, T.-C., Wong, G.T.F. and Hung, C.- C., Anal. Chem., 62, 774 (1990)
Iron removal from bacteria Trost, J.T. and Blankenship, R.E., Biochem., 28, 9898 (1989)
Trace metal ion removal from buffer solutions Yong, G., Leone,C. and Strothkamp, K.G., Biochem., 29, 9684 (1990). Severns, J.C.
and McMillin, D.R., Biochem., 29, 8592 (1990) Shang, S., Liao Y.-D., Wu, F., Y,-H. and Wu, C.- W., Biochem., 28, 9790 (1989), Chung, H.K. and Ingle, J.D., Anal. Chem., 62, 2547 (1990), Grimshaw, C.E., Shahbaz, M. and Putney, C.G., Biochem., 29, 9936 (1990).
Removal or multivalent cation contaminants Swanson, J.E., and Feigenson, G.W., Biochem., 29, 8291 (1990).
Purification of NMR reagents Brito, R.M.M., Rudolph, F.B., and Rosevear, P.R., Biochem., 30, 1461 (1991). Ray, W.J. Burgner, J.W., and Post, C.B., Biochem., 29, 2770 (1990)
Calcium removal from calmodulin Vorherr., T., James, P., Krebs, J., Enyedi, A., McCormick, D.J., Penniston, J.T., and Carafoli, E., Biochem., 29, 355 (1990)
Purification of zinc isotopes from human blood and excrement Gökmen, I.G., Aras, N.K., Gordon, G.E., Wastney, M.E., and Henkin, R.I., Anal. Chem., 61, 2757 (1989)
Calcium removal from buffer solutions Kinoshita, C.M., Ying, S.-C., Hugli, T.E., Siegel, J.N., Potempa., L.A., Jiang, H., Houghten, R.A. and Gewurz, H., Biochem., 28, 9840 (1989), Thielens, N.M., Dorsselaer, A.V., Gagnon, J. and Arlaud, G.J., Biochem., 29, 3570 (1990)
Removal of zinc from buffer solutions Jefferson, J.R., Hunt, J.B., and Ginsburg, A., Biochem., 29, 6687 (1990)
DNA Extraction for PCR Walsh, P.S., Metzger, D.A. and Higuchi, R., BioTechniques, 10, 506 (1991)
Stability of Selected Analytical Grade Resins to Various Treatments
Resin
Heat*
Solvents (Alcohols,
Hydrocarbons, etc.)
Oxidation
Reduction
AG 1 OH- form, fair to 50°C
Cl- and other forms,
good to 150°C
Very good Slowly dissolves in
hot 15% HNO3
or concentrated H2O2
Good**
AG 2 OH- form, fair to 30°C
Cl- form, good to 150°C
Very good Slowly dissolves in
hot 15% HNO3
Good**
AG 50W Good to 150°C Very good Slowly dissolves in
hot 15% HNO3
Very good
AG 4-X4 Good to 60°C Good Good Unknown
AG 501-X8 Good to 50°C Very good Slowly dissolves in
hot 15% HNO3
or concentrated H2O2
Good**
AG 11 A8 Good to 100°C Good Slowly dissolves in
hot 15% HNO3
or concentrated H2O2
Good
Bio-Rex 5 Good to 60°C Good Good Excellent
Bio-Rex 70 Good to 100°C Good Good Good
Bio-Rex MSZ 501(D) Good to 50°C Very good Slowly dissolves in
hot 15% HNO3
or concentrated H2O2
Good**
Chelex 100 Good to 75°C Good Breaks down in strong
oxidizing agents
Unknown
* All anion resins may be autoclaved except the CH- form; all cation exchange resins may be autoclaved.
** AG 1, AG 2, AG 501, and Bio-Rex MSZ 501 (D) resins will slowly break down in the presence of sulfur-containing reducing agents.
AG Resin Wet Mesh and Diameters
Wet mesh (U.S. Standards) 16 20 40 50 80 100 140 200 270 325 400
Diameter, µm 1,180 850 425 300 180 150 106 75 53 45 38

Chelex 100 resin is a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions. The carboxylic acid groups of Chelex 100 resin classify it as a weak cation exchange resin, but it differs from other exchangers in this class by featuring uniquely high selectivities for metal ions and much higher bond strengths. The resin can be used to ultrapurify buffers and ionic reagents: it will scavenge metal contaminants without altering the concentration of nonmetal ions.

Chelex 100 chelating ion exchange resin has a stronger selectivity for copper, iron, and other heavy metal ions over monovalent cations such as sodium and potassium. Its selectivity for divalent over monovalent ions is approximately 5,000 to 1, and it has a strong attraction for transition metals, even in highly concentrated salt solutions.

The following forms of Chelex 100 resin are available:

  • Analytical grade Chelex 100 resin — exhaustively sized, purified, and converted to make it suitable for accurate, reproducible analytical techniques
  • Biotechnology grade Chelex 100 resin — the analytical grade form certified to contain fewer than 100 microorganisms per gram of resin

Larger volumes and special packaging for industrial applications are available upon request.

Type Weak cation, chelating
Functional group R-CH2N(CH2COO-)2
Matrix Styrene divinylbenzene
Crosslinkage 1%
Approx. MW exclusion 3,500
Operating stability/pH range 4–14/0–14
Min. wet capacity (meq.ml) 0.4
Nominal density 0.65
Ionic forms Sodium, iron*
* Iron form is glyphosate concentration.  
Chelex® 100 Resin

142-2822
500 g, cation exchange resin, sodium form, 1% crosslinkage, 50–100 dry mesh size, 300–1,180 µm wet bead size, ~3,500 MW limit

List Price:   $743.00
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Chelex® 100 Resin

142-2832
500 g, cation exchange resin, sodium form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit

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Chelex® 100 Resin

142-2825
100 g, cation exchange resin, iron form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit

List Price:   $743.00
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Chelex® 100 Resin

142-1253
50 g, molecular biology grade cation exchange resin, sodium form, 1% crosslinkage, 200–400 dry mesh size, 75–150 µm wet bead size, ~3,500 MW limit

List Price:   $184.00
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Chelex® 100 Resin

143-2832
100 g, biotechnology grade cation exchange resin, sodium form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit

List Price:   $389.00
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Number Description Options
LIT237 Instruction Sheet, Chelex 100 Iron Form Resin for Glyphosate Cleanup, Rev B Click to download
LIT200 Instruction Manual, Chelex 100 and Chelex 20 Chelating Ion Exchange Resin, Rev B Click to download