Overview
Chelex 100 and COVID-19
Streamline your viral RNA preparation with Chelex 100 Resin
or InstaGene Matrix.
Chelex 100 Molecular Biology Grade Resin can be used for fast, easy nucleic acid preparations from many sample types including cell cultures, blood and tissues. DNA and RNA prepared using Chelex 100 is suitable for detection by PCR, RT-PCR, ddPCR or LAMP.
Chelex 100 chelates polyvalent metal ions, with a selectivity for divalent over monovalent ions of approximately 5,000 to 1. The resin avidly binds divalent cations such as Mg2+, limiting the activity of metallo-nucleases and other enzymes, as well as binding other polyvalent metal ions that interfere with enzyme-based applications such as PCR and ligation. Due to the high selectivity for divalent over monovalent ions, Chelex 100 Molecular Biology Grade Resin can be used for DNA and RNA preparations from samples with high levels of salts.
- Chelex 100 eliminates time-consuming and labor-intensive steps required with conventional nucleic acid extraction methods. A typical DNA or RNA preparation protocol involves: add Chelex 100 suspension to the sample, boil to release nucleic acids, spin to pellet resin beads and sample debris, use supernatant for downstream steps such as PCR or RT-PCR
- Molecular biology grade Chelex 100 Resin is certified to be endo- and exonuclease and ligase inhibitor-free
- Larger volumes and special packaging are available upon request
Chelex 100-based sample preparation workflow overview
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50 g, molecular biology grade cation exchange resin, sodium form, 1% cross-linkage, 200–400 dry mesh size, 75–150 µm wet bead size, ~3,500 MW limit, can be used for chromatography or molecular biology applications