Chelex® 100 Chelating Resin

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Chelex 100 Chelating Resin - Cation Exchange Resin

Overview

Analytical Grade Resin Wet Mesh and Equivalent Diameters

Wet mesh (U.S. Standards) 16 20 40 50 80 100 140 200 270 325 400
Diameter, µm 1,180 850 425 300 180 150 106 75 53 45 38
Analytical Grade Resin Selection Guide
Analytical Grade Resin Selection Guide
Relative Selectivity of Various Counterions

AG® MP-1 neutral species and cations do not interact with the resin. In the chloride form of AG1, AG MP-1, and AG2 resin, the counterion on the resin is C1. A resin can be converted from one ionic form to another. Ususally the resin is used in an ionic form with a lower selectivity for the functional group than the sample ions to be exchanged. The sample ions are then exchanged onto the resin when introduced, and can be eluted by introducing an ion with higher affinity for resin or a high concentration of an ion with equivalent or lower affinity. The table below shows the relative selectivity of various counterions. In general, the lower the selectivity of the counterion, the more readily it exchanges for another ion of like charge. The order of selectivity can also be used to estimate the effectiveness for different ions as eulants, with the most highly selective being the most efficient. Finally, the order of selectivity can be used to estimate the difficulty of converting the resin from one form to another. Conversion from a highly selected to a less highly selected form requires an excess of the new ion.

Relative Selectivity of Various Counterions
Counterion Relative Selectivity
for AG® 1 and
AG MP-1 Resins
Relative Selectivity
for AG 2 Resin
OH- 1.0 1.0
Benzene sulfonate 500 75
Salicylate 450 65
Citrate 220 23
I- 175 17
Phenate 110 27
HSO4- 85 15
CIO3- 74 12
NO3- 65 8
Br- 50 6
CN- 28 3
HSO3- 27 3
BrO3- 27 3
NO2- 24 3
Cl- 22 2.3
HCO3- 6.0 1.2
IO3- 5.5 0.5
HPO4- 5.0 0.5
Formate 4.6 0.5
Acetate 3.2 0.5
Propionate 2.6 0.3
F- 1.6 0.3

Chelex 100 resin is a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions. The carboxylic acid groups of Chelex 100 resin classify it as a weak cation exchange resin, but it differs from other exchangers in this class by featuring uniquely high selectivities for metal ions and much higher bond strengths.

Chelex 100 chelating ion exchange resin has a stronger selectivity for copper, iron, and other heavy metal ions over monovalent cations such as sodium and potassium. Its selectivity for divalent over monovalent ions is approximately 5,000 to 1, and it has a strong affinity for transition metals, even in highly concentrated salt solutions.

The following forms of Chelex 100 resin are available:

  • Analytical grade Chelex 100 resin — exhaustively sized, purified, and converted to make it suitable for accurate, reproducible analytical techniques
  • Biotechnology grade Chelex 100 resin — the analytical grade form certified to contain fewer than 100 microorganisms per gram of resin
Chelex 100 Resin

142-2822
500 g, cation exchange resin, sodium form, 1% crosslinkage, 50–100 dry mesh size, 300–1,180 µm wet bead size, ~3,500 MW limit

List Price:   $743.00
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Chelex 100 Resin

142-2832
500 g, cation exchange resin, sodium form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit

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Chelex 100 Resin

142-2842
500 g, cation exchange resin, sodium form, 1% crosslinkage, 200–400 dry mesh size, 75–150 µm wet bead size, ~3,500 MW limit

List Price:   $891.00
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Chelex 100 Resin

142-2825
100 g, cation exchange resin, iron form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit

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Chelex 100 Resin

142-1253
50 g, molecular biology grade cation exchange resin, sodium form, 1% crosslinkage, 200–400 dry mesh size, 75–150 µm wet bead size, ~3,500 MW limit

List Price:   $184.00
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Chelex 100 Resin

143-2832
100 g, biotechnology grade cation exchange resin, sodium form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit

List Price:   $389.00
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Number Description Options
LIT544 Instruction Manual, InstaGene Matrix, Rev G Click to download
LIT237 Instruction Sheet, Chelex 100 Iron Form Resin for Glyphosate Cleanup, Rev B Click to download
LIT200 Instruction Manual, Chelex 100 and Chelex 20 Chelating Ion Exchange Resin, Rev B Click to download [ Add to Cart (Free) ]