CHEF Genomic DNA Plug Kits

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CHEF Genomic DNA Plug Kits

Overview

Separation of E. coli Chromosomal Restriction Fragments

Separation of E. coli chromosomal restriction fragments. Lanes 1 and 14, S. cerevisiae chromosomes; lanes 2 and 13, lambda ladder; lanes 3–6, NotI restriction digests of chromosomal DNA from DH5αF' (lane 3), HB101 (lane 4), MC1061 (lane 5), MV1190 (lane 6); lanes 8–11, SfiI restriction digests of chromosomal DNA from DH5αF' (lane 8), HB101 (lane 9), MC1061 (lane 10), MV1190 (lane 11). DNA fragments were separated on a 1.0% pulsed field certified agarose gel in 0.5x TBE at 14°C for 26.4 hr at 6 V/cm (200 V) using a 120° included angle with a 2.77–26.32 sec linear switch-time ramp.

PFGE Standards Selection Guide
5 kb Ladder 8–48 kb Ladder Lambda Ladder S. cerevisiae
Chromosomal DNA
H. wingei
Chromosomal DNA
S. pombe
Chromosomal DNA
Comparison of Pulsed Field Standards
 
  Range Contents Amount Number of Applications
 
5 kb ladder 4.9–120 kb Concatemers of pBR328 20 µg in 200 µl 20–25
8–48 kb ladder 8.3–48.5 kb Mixed digest of phage λ 25 µg in 125 µl 125
Lambda (λ) ladder 0.05–1 Mb Concatemers of phage λcl857Sam7 5 agarose blocks 25–40
S. cerevisiae 0.225–2.2 Mb Saccharomyces cerevisiae chromosomal DNA 5 agarose blocks 25–40
H. wingei 1–3.1 Mb Hansenula wingei chromosomal DNA 5 agarose blocks 25–40
S. pombe 3.5–5.7 Mb Schizosaccharomyces pombe chromosomal DNA 5 agarose blocks 25–40
 

 

Certified Agarose Selection Guide
 
Packaging
25 g 161-3100 161-3103
161-3106   161-3111 161-3113 162-0137
100 g             162-0138
125 g 161-3101 161-3104 161-3107   161-3112 161-3114  
500 g 161-3102 161-3105       161-3115  
Analytical Separation
≥1,000 bp          
≤1,000 bp          
20–800 bp            
1 kb–2 Mb          
1 kb–5 Mb            
Preparative Separation
Quantum Prep methods      
Low-melt methods          
Agarose methods          
In-gel applications          
Other Applications
Postpreparative enzymatic treatments    
Tissue/cell culture            
Pulsed field sample preparation          
Blotting      
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CHEF genomic DNA plug kits provide a convenient means for preparing intact, chromosome-sized DNA for PFGE. With such large molecules, pipetting mechanically shears the DNA, resulting in unacceptable quality for PFGE separations. This problem has required procedures for lysis and deproteinization of whole cells embedded in agarose to yield intact purified DNA. Three kits are available for the preparation of bacterial (lysozyme-sensitive) or mammalian genomic DNA and yeast chromosomes (YACs). Each kit contains all the enzymes, reaction buffers, and restriction digest-qualified CleanCut agarose necessary to prepare 100 plugs, as well as disposable plug molds and screened caps for simplified plug processing. Each kit is thoroughly tested to ensure that prepared genomic DNA can be restriction digested and separated on a CHEF electrophoresis system.

CHEF Mammalian Genomic DNA Plug Kit  
Cell suspension buffer 12 ml     
>600 U/ml proteinase K 1.3 ml
Proteinase K reaction buffer 30 ml
2% CleanCut™ agarose 12 ml
10x wash buffer 60 ml
50-well disposable plug mold 2
Screened cap 1
CHEF Bacterial Genomic DNA Plug Kit  
Cell suspension buffer  12 ml 
>600 U/ml proteinase K  1.3 ml  
Proteinase K reaction buffer  30 ml 
2% CleanCut agarose  12 ml    
10x wash buffer  60 ml  
25 mg/ml lysozyme 1.6 ml     
Lysozyme buffer 30 ml  
50-well disposable plug mold 
Screened cap 
CHEF Yeast Genomic DNA Plug Kit  
Cell suspension buffer   12 ml  
>600 U/ml proteinase K      1.3 ml  
Proteinase K reaction buffer   30 ml  
2% CleanCut agarose   12 ml    
10x wash buffer   60 ml    
5,000 U/ml lyticase 1.6 ml   
Lyticase buffer 30 ml    
50-well disposable plug mold     2    
Screened cap 1    
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CHEF Mammalian Genomic DNA Plug Kit

1703591
Mammalian preparation kit for PFGE, includes proteinase K, CleanCut Agarose, all required buffers, screened cap, 2 plug molds, for 100 plugs

0 1703591 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3591_0921120435513.xml 1703591 09/21/09 12:04 PM N en 09/21/19 12:04 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:14 1703591-chef-mammalian-genomic-dna-plug-kit 1703591 CHEF Mammalian Genomic DNA Plug Kit CHEF Mammalian Genomic DNA Plug Kit Mammalian preparation kit for PFGE, includes proteinase K, CleanCut Agarose, all required buffers, screened cap, 2 plug molds, for 100 plugs Mammalian preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lyticase, all required buffers, screened cap, 2 plug molds, for 100 plugs Mammalian preparation kit for PFGE, includes proteinase K, CleanCut Agarose, all required buffers, screened cap, 2 plug molds, for 100 plugs /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-3591_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-3591_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-3591_feature.jpg preview RepEx 170-3591 Mammalian preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lyticase, all required buffers, screened cap, 2 plug molds, for 100 plugs 1703591, chef, mammalian, genomic, dna, plug, sample, preparation, pfge, pulsed, field, gel, electrophoresis 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis-- N 0 1703594 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3594_0921120435495.xml 1703605 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3605_0921120431250.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703591&CartQuote=QT&quantity= false true false $373.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703591&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703591&quantity= 1703591
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CHEF Bacterial Genomic DNA Plug Kit

1703592
Bacteria preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lysozyme, required buffers, screened cap, 2 plug molds, for 100 plugs

1 1703592 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3592_0921120435403.xml 1703592 09/21/09 12:04 PM N en 09/21/19 12:04 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:14 1703592-chef-bacterial-genomic-dna-plug-kit 1703592 CHEF Bacterial Genomic DNA Plug Kit CHEF Bacterial Genomic DNA Plug Kit Bacteria preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lysozyme, required buffers, screened cap, 2 plug molds, for 100 plugs Bacteria preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lysozyme, required buffers, screened cap, 2 plug molds, for 100 plugs Bacteria preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lysozyme, required buffers, screened cap, 2 plug molds, for 100 plugs /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-3592_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-3592_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-3592_feature.jpg RepEx 170-3592 Bacteria preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lysozyme, required buffers, screened cap, 2 plug molds, for 100 plugs 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis-- N 0 1703592, chef, bacterial, genomic, dna, plug, sample, preparation, pfge, pulsed, field, gel, electrophoresis 1703605 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3605_0921120431250.xml 1703594 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3594_0921120435495.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703592&CartQuote=QT&quantity= false true false $494.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703592&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703592&quantity= 1703592
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CHEF Yeast Genomic DNA Plug Kit

1703593
Yeast preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lyticase, all required buffers, screened cap, and 2 plug molds, for 100 plugs

2 1703593 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3593_0921120435590.xml 1703593 09/21/09 12:04 PM N en 09/21/19 12:04 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:14 1703593-chef-yeast-genomic-dna-plug-kit 1703593 CHEF Yeast Genomic DNA Plug Kit CHEF Yeast Genomic DNA Plug Kit Yeast preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lyticase, all required buffers, screened cap, and 2 plug molds, for 100 plugs Yeast preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lyticase, all required buffers, screened cap, and 2 plug molds, for 100 plugs Yeast preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lyticase, all required buffers, screened cap, and 2 plug molds, for 100 plugs /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-3593_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-3593_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-3593_feature.jpg RepEx 170-3593 Yeast preparation kit for PFGE, includes proteinase K, CleanCut Agarose, lyticase, all required buffers, screened cap, and 2 plug molds, for 100 plugs 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis-- N 0 1703593, chef, yeast, genomic, dna, plug, sample, preparation, pfge, pulsed, field, gel, electrophoresis 1703605 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3605_0921120431250.xml 1703594 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3594_0921120435495.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703593&CartQuote=QT&quantity= false true false $567.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703593&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703593&quantity= 1703593
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$567.00 
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CleanCut Agarose

1703594
12 ml, 2% agarose for pulsed field gel electrophoresis (PFGE), restriction digest-qualified, for 100 plugs

3 1703594 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3594_0921120435495.xml 1703594 09/21/09 12:04 PM N en 09/21/19 12:04 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:14 1703594-cleancut-agarose 1703594 CleanCut Agarose CleanCut Agarose 12 ml, 2% agarose for pulsed field gel electrophoresis (PFGE), restriction digest-qualified, for 100 plugs 12 ml, 2% agarose for pulsed field gel electrophoresis (PFGE), restriction digest-qualified, for 100 plugs 12 ml, 2% agarose for pulsed field gel electrophoresis (PFGE), restriction digest-qualified, for 100 plugs /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-3594_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-3594_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-3594_feature.jpg RepEx 170-3594 12 ml, 2% agarose for pulsed field gel electrophoresis (PFGE), restriction digest-qualified, for 100 plugs 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis-- N 0 1703594, cleancut, agarose, 2%, pulsed, field, gel, electrophoresis, pfge, restriction, digest-qualified 1703605 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3605_0921120431250.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703594&CartQuote=QT&quantity= false true false $32.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703594&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703594&quantity= 1703594
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CHEF Mapper XA Chiller System

1703670
120 V, complete PFGE system, includes cooling module, pump, tubing, S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose

1703670 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3670_0921120430749.xml 4 1703670 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jcjsy03rjcjsy03s" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jcjsy03rjcjsy03s"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="22" DayOfWeek="1" Year="2018" Hour="19" Minute="29" Second="18" ZoneOffsetMillis="0" Time="1516649358756">Mon Jan 22 19:29:18 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3670_0921120430749.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="22" DayOfWeek="1" Year="2018" Hour="19" Minute="29" Second="18" ZoneOffsetMillis="0" Time="1516649358756">Mon Jan 22 19:29:18 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:04 PM en_US jcc1tow1 2017-11-14 07:08:14 true true internet/product_SKU LSR N en 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:08:14 1703670-chef-mapper-xa-chiller-system 1703670 CHEF Mapper XA Chiller System CHEF Mapper XA Chiller System 120 V, complete PFGE system, includes cooling module, pump, tubing, <em>S. cerevisiae</em> DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose 120 V, complete PFGE system, includes cooling module, pump, tubing, <em>S. cerevisiae</em> DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose 120 V, complete PFGE system, includes cooling module, pump, tubing, <em>S. cerevisiae</em> DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-3670_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-3670_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-3670_feature.jpg RepEx 170-3670 120 V, complete PFGE system, includes cooling module, pump, tubing, <em>S. cerevisiae</em> DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis-- N 0 1703670, chef, mapper, xa, chiller, complete, pfge, pulsed, field, gel, electrophoresis, reagents, instrument, 120, 120V, volts 1703592 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3592_0921120435403.xml 1703591 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3591_0921120435513.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703670&CartQuote=QT&quantity= false true false null false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703670&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703670&quantity= 1703670
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CHEF-DR III Chiller System

1703700
120 V, complete variable angle PFGE system with cooling module, includes all gel casting accessories, pump, tubing, DNA standards, 2 plugs of S. cerevisiae DNA size standard, PFGE grade agarose

1703700 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3700_0921120431209.xml 10 1703700 09/21/09 12:04 PM en_US jcc1tovd 2017-11-14 07:08:14 true true internet/product_SKU LSR N en 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:08:14 1703700-chef-dr-iii-chiller-system 1703700 CHEF-DR III Chiller System CHEF-DR III Chiller System 120 V, complete variable angle PFGE system with cooling module, includes all gel casting accessories, pump, tubing, DNA standards, 2 plugs of <em>S. cerevisiae</em> DNA size standard, PFGE grade agarose 120 V, complete variable angle PFGE system with cooling module, includes all gel casting accessories, pump, tubing, DNA standards, 2 plugs of <em>S. cerevisiae</em> DNA size standard, PFGE grade agarose 120 V, complete variable angle PFGE system with cooling module, includes all gel casting accessories, pump, tubing, DNA standards, 2 plugs of <em>S. cerevisiae</em> DNA size standard, PFGE grade agarose /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-3700_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-3700_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-3700_feature.jpg RepEx 170-3700 120 V, complete variable angle PFGE system with cooling module, includes all gel casting accessories, pump, tubing, DNA standards, 2 plugs of <em>S. cerevisiae</em> DNA size standard, PFGE grade agarose 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis-- N 0 1703700, chef-dr, iii, chiller, complete, variable, angle, pfge, pulsed, field, gel, electrophoresis, casting, accessories, reagents, instrument, 120, 120V, volts 1703591 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3591_0921120435513.xml 1703592 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3592_0921120435403.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703700&CartQuote=QT&quantity= false true false null false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703700&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703700&quantity= 1703700
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CHEF-DR II Chiller System

1703725
120 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose

1703725 /templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3725_0921120430970.xml 15 1703725 <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jcjsxzbvjcjsxzbw" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jcjsxzbvjcjsxzbw"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="19" DayOfWeek="5" Year="2018" Hour="21" Minute="53" Second="7" ZoneOffsetMillis="0" Time="1516398787396">Fri Jan 19 21:53:07 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis--/170-3725_0921120430970.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="19" DayOfWeek="5" Year="2018" Hour="21" Minute="53" Second="7" ZoneOffsetMillis="0" Time="1516398787396">Fri Jan 19 21:53:07 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:04 PM en_US jcc1towa 2017-11-14 07:08:14 true true internet/product_SKU LSR N en 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:08:14 1703725-chef-dr-ii-chiller-system 1703725 CHEF-DR II Chiller System CHEF-DR II Chiller System 120 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs <em>S. cerevisiae</em> DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose 120 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs <em>S. cerevisiae</em> DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose 120 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs <em>S. cerevisiae</em> DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose /webroot/web/images/lsr/products/electrophoresis/sku_thumb/global/170-3725_tn.jpg /webroot/web/images/lsr/products/electrophoresis/sku_view/global/170-3725_view.jpg /webroot/web/images/lsr/products/electrophoresis/sku_feature/global/170-3725_feature.jpg RepEx 170-3725 120 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs <em>S. cerevisiae</em> DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose 09/21/09 12:04 PM 09/21/19 12:04 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Electrophoresis-and-Blotting/Nucleic-Acid-Electrophoresis-- N 0 1703725, chef-dr, ii, chiller, complete, pfge, pulsed, field, gel, electrophoresis, casting, accessories, reagents, instrument, 120, 120V, volts false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703725&CartQuote=QT&quantity= false true false null false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703725&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1703725&quantity= 1703725
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Number Description Options
1753 Preparation and Restriction Digestion of Escherichia coli Chromosomal DNA in Agarose Plugs for Use in PFGE Bulletin, Rev A Click to download
LIT510 Instruction Manual, CHEF Genomic DNA Plug Kit, Rev E Click to download
5901 Pulsed Field Gel Electrophoresis Brochure, Rev B Click to download [ Add to Cart (Free) ]
6228 Using Pulsed Field Gel Electrophoresis Standards, Rev A Click to download

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