CFX96 Touch™ Real-Time PCR Detection System

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CFX96 Touch™ Real-Time PCR Detection System

Overview

1000-series Touch Family System Tour
Excitation and Detection Wavelength Charts
Excitation

Detection

Excitation and detection wavelength ranges for the CFX96 Touch™ and CFX96 Touch Deep Well™ systems.

Optical Design
CFX96 systems' solid-state optical technology (six filtered LEDs and six filtered photodiodes)

Innovative Optical Design
The CFX96 Touch™ and CFX96 Touch Deep Well™ systems' solid-state optical technology (six filtered LEDs and six filtered photodiodes) provides sensitive detection for precise quantification and target discrimination.

Scanning just above the sample plate, the optics shuttle individually illuminates and detects fluorescence from each well with high sensitivity and no cross talk. The optical system automatically collects data from all wells during data acquisition, so you can enter or edit well information on your own schedule.

Five-Target Multiplexing
The CFX96 Touch and CFX96 Touch Deep Well systems can discriminate up to five targets in a single reaction well. The optical filter sets are designed to maximize fluorescence detection for specific dyes in specific channels. At every position and with every scan, the optics shuttle is reproducibly centered above each well, so the light path is always fixed and optimal, and there is no need to sacrifice data collection in one of the channels to normalize to a passive reference.

Fast Scan Option
You can complete SYBR® Green I and single-color FAM protocols even faster using the fast scan mode, which reads single-channel fluorescence from all 96 wells in just 3 seconds.

Fluorescence Resonance Energy Transfer (FRET)
The CFX96 Touch and CFX96 Touch Deep Well systems' optics shuttle includes one channel with an LED-filter photodiode combination dedicated for FRET singleplex experiments, further expanding your experimental options.

Fast Thermal Cycling

If you're like most researchers, you want to get results faster. Many factors contribute to the overall time it takes to get PCR results: the time to design, program, and optimize a protocol, the time to run the protocol, and the time to analyze the results. Bio-Rad engineers have developed a complete thermal cycling system that will speed every step of the PCR process to give you what's really important — a shorter time from setup to results.

Protocol autowriter — generates an optimal protocol based on your polymerase, primers, and product length.

Thermal gradient feature — identifies optimal annealing temperature in a single run.

Reduced-mass sample block — fast ramping and settling produce the shortest time to target temperature available in a thermal cycler.

Intuitive software — makes programming faster and easier for PCR and real-time PCR.

The patent-pending reduced-mass sample block heats and cools more quickly than standard blocks, so average ramp rates are increased and overall run times are reduced

The patented* reduced-mass sample block heats and cools more quickly than standard blocks, so average ramp rates are increased and overall run times are reduced.

Superior Uniformity
Precision of the temperature steps is critical for the rate and efficiency of PCR. To obtain reliable, consistent results, all sample wells must maintain proper temperature throughout each incubation step. The CFX96 Touch™ system uses six independently controlled thermal electric modules (TEs), the heating and cooling elements of the thermal cycler, to maintain tight temperature uniformity at all points during a run — even while ramping.

Rapid Arrival at Target Temperature
A key component of overall protocol run time is the time required to reach target temperature, which is determined by the average ramp rate and the time needed for the sample block to reach thermal uniformity. Maximum ramp rate is less important because it can fluctuate significantly during the ramp. The CFX96 Touch system produces high average ramp rates and tight uniformity during ramping to yield fast time to target temperature and faster protocol run times. Run times can be dramatically shortened — to less than 30 min — while still producing accurate quantitative results. Now you can tailor your runs around your schedule instead of tailoring your schedule around your runs.

Rapid arrival at target temperature and superior uniformity

Superior uniformity with rapid arrival at target temperature. 1000-series thermal cyclers exhibit high average ramp rates, rapid settling time, and tight thermal uniformity throughout the ramp. This graph shows the temperature measured by probes in 15 wells across a sample block. The traces are nearly indistinguishable due to the tight uniformity. Note the consistent high average ramp rate throughout heating and cooling.

* U.S. patent 7,632,464.

Thermal Gradient

Efficient Optimization
Determining the optimal temperature for primer annealing is crucial for efficient and specific amplification of product. With the CFX96 Touch systems' thermal gradient feature, you can determine the optimal temperature for primer annealing in a single experiment, minimizing the use of precious samples and reagents and saving valuable research time. At any step in a protocol, you can program a temperature gradient of up to 24°C across the reaction block. The thermal cycler provides exceptional temperature uniformity and reproducibility within each gradient zone, and the temperatures can easily be programmed and viewed on the touchscreen or onscreen in the software so you can quickly identify the optimal incubation temperature.


Thermal gradient experiment for optimizing annealing temperature. A tenfold dilution series (106 to 10 copies) of plasmid containing GAPDH template was amplified in the presence of SYBR® Green dye using a protocol with an annealing thermal gradient ranging from 55 to 68°C. Results are presented for three temperatures, showing 62°C as the optimal in this case, with early quantification cycle (Cq) values and the highest standard curve efficiency. RFU, relative fluorescence units.


O-ring hermetic seal extends the life of the heating and cooling elements. Condensation from constant heating and cooling can degrade thermal electric modules (TEs), causing them to fail. Bio-Rad 1000-series cyclers include a patented* O-ring seal (red in illustration) that forms an airtight barrier around the TEs, preventing exposure to condensation. This substantially lengthens the life of TEs and guarantees outstanding thermal performance.

* U.S. patent 7,051,536.

Flexibility

The Ultimate in Configuration Flexibility

A System That Grows with Your Research
The flexibility of the 1000-series platform allows your setup to grow and evolve as your needs change. Adding more instruments to increase throughput or expand options is as easy as connecting a USB cable. CFX Manager™ software can independently run four instruments, including a combination of CFX96 Touch™, CFX96 Touch Deep Well™, or CFX384 Touch™ real-time instruments and C1000 Touch™ or S1000™ thermal cyclers. You may start a run on one instrument while another is running a different experiment, or you can start the same experiment on all four instruments at once.

CFX96 Connections

Configuration Options

Stand-Alone

  • Stand-alone thermal cyclers and real-time PCR detection systems

 


Stand-Alone Multi-Instrument

  • C1000 Touch cycler control of up to 3 S1000 cyclers
  • CFX96 Touch, CFX96 Touch Deep Well, or CFX384 Touch system control of up to 3 S1000 cyclers

 


Software-Controlled Stand-Alone

  • C1000 Touch cycler or CFX96 Touch, CFX96 Touch Deep Well, or CFX384 Touch system controlled by CFX Manager software

 


Software-Controlled Multi-Instrument

  • Up to 8 C1000 Touch cyclers, each connected to 1–3 S1000 cyclers, controlled by CFX Manager software
  • 1 CFX96 Touch, CFX96 Touch Deep Well, or CFX384 Touch system connected to 1–3 S1000 cyclers, controlled by CFX Manager software
  • Up to 4 CFX96 Touch, CFX96 Touch Deep Well, and/or CFX384 Touch systems controlled by CFX Manager software

 

Performance

 

Excellent uniformity. IL-1β plasmid template diluted to 105 copies/reaction amplified in the presence of a FAM-labeled detection probe with iQ™ supermix. Graph shows 96 replicates of 10 µl reactions. Average quantification cycle (Cq) = 19.81 ± 0.10. RFU, relative fluorescence units.

 

Confidently analyze data from a broad range of sample concentrations even when multiplexing five targets. A–E, fluorescence data from a series of tenfold dilutions of plasmid DNA (108–102 copies) amplified using reporter dyes to monitor five targets: , FAM/actin; , HEX/GAPDH; , Texas Red/cyclophilin; , Cy5/tubulin; , Quasar 705/IL-1β; F, standard curves generated from data in A–E, reaction efficiencies range from 97 to 103%. Cq, quantification cycle; RFU, relative fluorescence units.

qbasePLUS Software

Biogazelle and Bio-Rad have teamed up to offer a powerful suite of tools that will speed up real-time PCR data analysis and accelerate discovery.

QbasePLUS Softare

Key features of qbasePLUS software:

  • Reliable validation — based on proven solutions for quality control, normalization, and inter-run calibration
  • Efficient data analysis — import and consolidate information from multiple runs and multiple instruments to quickly analyze your complete data set, and use a guided statistical wizard to determine significance
  • Streamlined publication submission — export an RDML file containing annotations, such as sample and assay information, to conform to the MIQE guidelines
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Plastics Compatibility Chart

Instrument Compatibility of PCR Plastic Consumables

 

Real-Time PCR Detection Systems
  MiniOpticon System
MiniOpticon™ System
MyiQ2 and iCycler iQ Systems
iCycler iQ®, iQ™5,
MyiQ™, and MyiQ™2 Systems
Chromo4 System
Chromo4™ System
DNA Engine Opticon 2 System
DNA Engine Opticon® 2 System

CFX96 Touch™, CFX96 Touch Deep Well™, CFX384
Touch™, and CFX Connect™ Systems
Tubes
High-profile individual tubes   CFX96 Touch
Deep Well only
Low-profile tube strips  
48- and 96-Well Plates
Hard-Shell® low-profile 96-well semi-skirted PCR plates        
Hard-Shell high-profile 96-well semi-skirted PCR plates     CFX96 Touch
Deep Well only
Hard-Shell low-profile 96-well skirted PCR plates    
Multiplate™ high-profile unskirted PCR plates   CFX96 Touch
Deep Well only
Multiplate low-profile unskirted PCR plates  
iQ™ 96-well semi-skirted PCR plates      
384-Well Plates
Hard-Shell 384-well skirted PCR plates        
Lids and Adhesive Seals
Optical flat cap strips
Microseal® 'B' adhesive seals  
Microseal® ꞌCꞌ optical seals  
Heat Sealer and Optically Clear Heat Seal
PX1™ PCR plate sealer  
Optically clear heat seal  
qPCR Applications Guide
qPCR Applications Guide

The qPCR Applications Guide is a comprehensive source of information on real-time PCR. Complete with detailed information on optimizing, validating, and analyzing real-time PCR data, this technically focused applications guide is a resource for experienced researchers as well as those wanting to learn more about using real-time PCR for applications such as qPCR, RT-qPCR, genotyping, and GMO detection. This guidebook includes chapters that cover:

  • Key concepts of real-time PCR
  • Getting started — experimental design
  • Multiplexing considerations
  • Data analysis
  • Gene expression analysis
  • Genotyping allelic discrimination
  • GMO detection

This guidebook comes with an easy-to-review real-time PCR quick guide to use at the bench. The quick guide covers the fundamentals of qPCR, design, optimization, and data analysis of qPCR experiments.

The CFX96™ optical reaction module converts the C1000 Touch™ thermal cycler chassis into the powerful and precise CFX96 Touch real-time PCR detection system. This six-channel system combines advanced optical technology with precise thermal control to deliver sensitive, reliable detection. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen. With up to five-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX96 Touch system is designed to advance your qPCR.

Key Features and Benefits

With the CFX96 Touch real-time PCR detection system you can:

  • Set up your system quickly — easy installation and factory-calibrated optics
  • Minimize sample and reagent use — up to 5-target multiplexing with sample volumes as low as 10 µl
  • Optimize reactions in a single run — thermal gradient feature
  • Analyze data faster — visualize all run data at once and export only the data you need in the format you want
  • Use advanced data analysis tools — normalized gene expression using CFX Manager™ software
  • Configure the system to fit your needs — run without a computer, run up to 4 systems from 1 computer, or integrate with the CFX automation system for higher throughput
  • Streamline publication submission — include MIQE annotations and generate RDML files using Biogazelle's qbasePLUS software

CFX96 Touch and CFX384 Touch™ Firmware

Current version: PXA270 1.101.422.514, HC12 1.0.165.0.A, DSP 1.123, and 8051 Smartlid 0.54

Thermal Cycler  
Chassis C1000 Touch™
Maximum ramp rate, °C/sec 5
Average ramp rate, °C/sec 3.3
Heating and cooling method Peltier
Lid, °C Heats up to 105
Temperature  
Range, °C 0–100
Accuracy, °C ±0.2 of programmed target at 90°C
Uniformity, °C ±0.4 well-to-well within 10 sec of arrival at 90°C
Gradient  
Operational range, °C 30–100
Programmable span, °C 1–24
Optical Detection  
Excitation 6 filtered LEDs
Detection 6 filtered photodiodes
Range of excitation/emission wavelengths, nm 450–730
Sensitivity Detects 1 copy of target sequence in human genomic DNA
Dynamic range 10 orders of magnitude
Scan Time  
All channels, sec 12
FAM/SYBR® Green only, sec 3
Software  
Operating systems Windows XP, Windows 7
Multiplex analysis Up to 5 targets per well
System  
Licensed for real-time PCR Yes
Sample capacity, wells 96
Sample size, µl 1–50 (10–25 recommended)
Communication interface USB 2.0
Electrical approvals IEC, CE
Dimensions (W x D x H), cm/in 33 x 46 x 36 /13 x 18 x 14
Weight, kg/lb 21/47
CFX96 Touch™ Real-Time PCR Detection System

185-5196
Modular thermal cycler platform, includes C1000 Touch™ thermal cycler chassis, CFX96™ optical reaction module, CFX Manager™ software, qbasePLUS software license, cables, reagents, consumables

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CFX96 Touch™ Real-Time PCR Detection System

185-5195
Modular thermal cycler platform, includes C1000 Touch™ thermal cycler chassis, CFX96™ optical reaction module, CFX Manager™ software, qbasePLUS software license, cables

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promo  
CFX96™ Optical Reaction Module

184-5096
96-well optical module for real-time PCR, includes CFX Manager™ software, qbasePLUS software license, cables, reagents, consumables, for use with C1000 Touch™ thermal cycler chassis

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CFX96™ Optical Reaction Module

184-5097
96-well optical module for real-time PCR, includes CFX Manager™ software, qbasePLUS software license, cables, for use with C1000 Touch™ thermal cycler chassis

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Number Description Options
10022527 Amplification Plastics and Reagents Selection Guide, Rev B Click to download
6094 1000-Series Thermal Cyclers Brochure, Rev B Click to download [ Add to Cart (Free) ]
6093 CFX96 Touch Real-Time PCR Detection System Brochure, Rev C Click to download [ Add to Cart (Free) ]
6075 CFX96 Touch Real-Time PCR Detection System Specifications Sheet, Rev B Click to download [ Add to Cart (Free) ]
6076 CFX96 Touch Real-Time PCR Detection System Flier, Rev A Click to download [ Add to Cart (Free) ]
6047 Optical Design of CFX96 Real-Time PCR Detection System Eliminates the Requirement of a Passive Reference Dye, Rev A Click to download [ Add to Cart (Free) ]
6009 High Resolution Melt Parameter Considerations for Optimal Data Resolution, Rev A Click to download [ Add to Cart (Free) ]
6004 A Practical Guide to High Resolution Melt Analysis Genotyping, Rev A Click to download [ Add to Cart (Free) ]
5990 Gene Expression Analysis Solutions Brochure, Rev C Click to download [ Add to Cart (Free) ]
10010424 Instruction Manual, CFX96 and CFX384 Real-Time PCR Detection Systems, Rev D Click to download
10021337 Instruction Manual, CFX96 Touch, CFX96 Touch Deep Well, CFX Connect, and CFX384 Touch Real-Time PCR Detection Systems, Rev E Click to download
10022138 Instruction Manual, Checking Firmware and Software Updates for the C1000 Touch Thermal Cycler and the CFX96 Touch, CFX96 Touch Deep Well, and CFX384 Touch Real-Time PCR Detection Systems — A Quick Guide, Rev B Click to download
5690 CFX Manager Software, Security Edition Product Information Sheet, Rev A Click to download
10016883 Transferring Data from CFX Manager Software to qbasePLUS Software — Quick Guide, Rev D Click to download
5859 A Practical Approach to RT-qPCR — Publishing Data That Conform to the MIQE Guidelines, Rev D Click to download [ Add to Cart (Free) ]
10021418 Instruction Manual, CFX96 Touch and CFX96 Touch Deep Well Real-Time PCR Detection Systems installation Quick Guide, Rev B Click to download
10010420 CFX Manager Software Protocol — Quick Guide, Rev C (for CFX96) Click to download
10010421 CFX Manager Software Plate — Quick Guide, Rev C (for CFX96) Click to download
10010422 CFX Manager Software Data Analysis — Quick Guide, Rev C (for CFX96) Click to download
10010423 CFX Manager Software Gene Expression Analysis — Quick Guide, Rev D (for CFX96) Click to download
5962 Fast Optimization of a Multiplex Influenza Identification Panel Using a Thermal Gradient, Rev A Click to download
5692 Real-Time qPCR as a Tool for Evaluating RNAi-Mediated Gene Silencing, Rev A (tech note) Click to download
10018778 SsoFast EvaGreen Control Assay Instructions, Rev A Click to download
5973 SsoFast EvaGreen Control Assay Flier, Rev A Click to download
10027341 CFX Qualification Plate — 96-Well Plate Format Product Insert, Rev B Click to download
5279 Real-Time PCR Applications Guide, Rev B Click to download [ Add to Cart (Free) ]