Cell Lysis RT-qPCR Kits

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RT-qPCR from Cell Lysate

Overview

High-Quality Gene Expression from Precious Samples — A Simplified Workflow

Obtaining enough starting material to complete multiple gene expression experiments can be challenging when working with precious and limited samples. See how Mark Kibschull of the Lunenfeld-Tanenbaum Research Institute in Toronto determines pluripotency and differentiation of limited human embryonic stem cells using Bio-Rad's SingleShot™ Cell Lysis Kit, SsoAdvanced™ PreAmp Supermix, and PrimePCR™ Validated Primer Panels. This simplified workflow allows for the gene expression analysis of hundreds of genes from as little as 100 human embryonic stem cells, and without the cumbersome step of isolating and purifying RNA.

Gene Expression Data from Cell Lysates and Purified RNA

SingleShot™ Probes One-Step Kit lysates and purified RNA yield comparable gene expression results

SingleShot™ Probes One-Step Kit demonstrated superior correlation with an R2 of 0.9954 compared to the same samples, but purified using a column-based method. FastLane Cell Probe Kit from Qiagen demonstrated a less than optimal correlation with an R2 of 0.9307 compared to the same samples, but purified using a column-based method.
SingleShot Probes One-Step Kit (Bio-Rad) Cq values show high correlation (R2 = 0.9954) with Cq values generated from purified RNA. FastLane Cell Probe Kit (QIAGEN) lysates Cq values demonstrate less than optimal correlation (R2 = 0.9307) with Cq values generated from purified RNA.

Gene expression studies conducted using different sample preparation methods should yield the same relative results. The expression of five genes was assessed in a tenfold dilution series spanning 10–105 input cells to compare the use of lysate vs. purified RNA as input for real-time PCR. Cq, quantification cycle.

The SingleShot™ Cell Lysis Kit Shows Superior RNase Inhibition

Fluorescence detection of RNases

Degraded RNA transcripts may lead to less than optimal qPCR data. This assay detected the presence of RNases via fluorescence detection after the lysis solution was added but prior to heat treatment.

Fluorescence detection of RNases

The lysis solution in Bio-Rad’s SingleShot Cell Lysis Kit contains a potent RNase inhibitor, which preserves RNA integrity. Life Technologies’ RNaseAlert QC System Assay showed the presence of high levels of RNases in the FastLane Cell cDNA kit, resulting in RNA degradation. A water control was added for baseline determination. RFU, relative fluorescence units.

The SingleShot™ SYBR® Green Kit Efficiently Removes Genomic DNA

104–105 input cells were evaluated for gDNA carryover after DNase treatment in three different cell lines (HeLa, NCI-H1975, and NIH 3T3).

The SingleShot Kit completely removed gDNA. No amplification (Cq >40) was detected from the DNA control assay regardless of the starting amount of input cells.

The competitor kit amplified gDNA at high levels across all three cell lines at all starting amounts of input cells, which can cause inaccurate gene expression data because both cDNA and gDNA would be amplified.

Accurate gene expression studies are based on relative expression levels of RNA transcripts

Cq, quantification cycle; NCI, National Cancer Institute; NIH, National Institutes of Health.

Reproducibility of Technical Lysate Replicates Ensures Optimal Data

The SingleShot™ SYBR® Green Kit demonstrates high reproducibility between lysate replicates, indicated by a Pearson correlation of 0.99.

Two technical lysate replicates were examined across 4 neuroblastoma cell lines (SH-EP, SK-N-AS, NGP, IMR-32) using 10 SYBR® Green qPCR assays to evaluate the reproducibility of the SingleShot SYBR® Green Kit

Two technical lysate replicates of four neuroblastoma cell lines (SH-EP, SK-N-AS, NGP, and IMR-32) were examined using ten SYBR® Green qPCR assays to evaluate the reproducibility of the SingleShot™ SYBR® Green Kit. Cq, quantification cycle.

Data used with permission from Gert Van Peer, Pieter Mestdagh, and Jo Vandesompele, Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium.

Optimal Efficiency and Dynamic Range across Expression Ranges

PCR Efficiency

Three targets were analyzed using input cell numbers ranging from 10 to 105 HeLa cells.

PCR efficiencies greater than 98% were maintained regardless of the expression levels, and sensitivity down to 10 cells was observed.

Accurate gene expression studies require that PCR efficiencies are within 90-110% per the MIQE guidelines.

Accurate gene expression studies require that PCR efficiencies be within 90–110% per the minimum information for publication of quantitative real-time PCR experiments (MIQE) guidelines. The SingleShot RNA control was used to monitor PCR inhibition. As the control quantification cycle (Cq) values remained constant, no inhibition was noted.


SingleShot™ SYBR® Green One-Step Kit enables a large dynamic range

Two technical lysate replicates were examined across three differentially expressed genes (B2M, HPRT, TBP) using a human kidney tumor (HKT) cell line.

The SingleShot™ SYBR® Green One-Step Kit showed a large dynamic range across all assayed expression levels.

The SingleShot™ SYBR® Green One-Step Kit showed a large dynamic range across all assayed expression levels

Cq, quantification cycle.

Dynamic Ranges of SingleShot™ One-Step Kit Lysates and Isolated RNA

SingleShot™ SYBR® Green One-Step Kit lysates yield a dynamic range similar to purified RNA

Gene expression data were generated from a 10–105 dilution series of K562 human cell line and compared using SingleShot™ SYBR® Green One-Step Kit lysates and isolated RNA (Aurum™ Total RNA Mini Kit).

B2M expression was assessed using a PrimePCR™ qPCR Assay. The lysates were generated without the need for RNA isolation.

Equal Performance and Superior Sensitivity Compared to Isolated RNA

 

SingleShot™ Probes One-Step Kit lysates yield a dynamic range similar to purified RNA

Gene expression data were generated from a 10–105 dilution series of K562 human cell line and compared using SingleShot Probes One-Step Kit lysates and isolated RNA (Aurum Total RNA Mini Kit).

TBP expression was assessed using a PrimePCR™ qPCR Assay. The lysates were generated without the need for RNA isolation.

Dynamic Range of SingleShot™ Probes One-Step Kit Lysates vs. RNA

RFU, relative fluorescence units.

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SingleShot™ Cell Lysis RT-qPCR Kits provide a complete and fast solution for the generation of lysates from cell cultures, optimized for downstream one step or two step quantitative PCR (qPCR) reactions, without an RNA purification step.

Single Shot Workflow

Kits are available in SYBR® Green or probe chemistries. A cell lysis kit is also offered as a stand-alone option.

All kits offer the following key benefits:

  • SingleShot RNA Control ensures optimal cell and lysate inputs

Choose the Right Kit for Your Needs

SingleShot Kit Features Product Options
Cell Lysis One-Step RT-qPCR Kits
  • Cell lysis, reverse transcription, and qPCR
  • All one-step RT-qPCR reagents included
  • No RNA purification needed
SYBR® Green
100 reactions
(1725095)
Probes
100 reactions
(1725070)
Cell Lysis Two-Step RT-qPCR Kits
  • Cell lysis, reverse transcription, and qPCR
  • All two-step RT-qPCR reagents included
  • No RNA purification needed
SYBR® Green
100 reactions
(1725085)
Probes
100 reactions
(1725090)
Cell Lysis Kits
  • Cell lysis for RT-qPCR
  • RT-qPCR reagents sold separately
  • No RNA purification needed
100 reactions
(1725080)
500 reactions
(1725081)
 

Simple Workflow

Simple, short, minimal hands-on protocol is suitable for automation and provides a high-throughput solution.

Grow cells to desired density, transfer up to 105 cells to wells of a PCR plate, add lysis solution, and incubate at room temperature for 10 minutes.

Heat for 5 minutes at 37°C and then for 5 minutes at 75°C. The lysate is ready for RT-qPCR or can be stored for future use.

Preamp Single Shot Workflow

Cell Lines Validated for Use with the SingleShot Cell Lysis RT-qPCR Kits

All SingleShot Cell Lysis RT-qPCR Kits have been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:

Cell Line Property Species
A549 Adherent H. sapiens, lung carcinoma
HeLa Adherent H. sapiens, cervical adenocarcinoma
HepG2   Adherent  H. sapiens, liver carcinoma
NIH/3T3 Adherent M. musculus, embryonic fibroblast
MCF-7 Adherent  H. sapiens, epithelial, adenocarcinoma
NCI-H1975  Adherent H. sapiens, lung adenocarcinoma 
K-562 Suspension H. sapiens, chronic myelogenous leukemia
HEK 293 Suspension H. sapiens, kidney
Jurkat Suspension H. sapiens, T lymphocyte
PC-12  Suspension R. norvegicus, pheochromocytoma, adrenal
HepRG  Primary-like H. sapiens, hepatocytes

Related Categories

Supporting Documentation

Online Resources

Storage at 4°C 6 months:
Lysis buffer

3 months:
iTaq™ Universal One-Step Reaction Mix
SsoAdvanced™ Universal Supermix
Storage at –20°C All kit components are stable for 12 months
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SingleShot™ Cell Lysis Kit, 100 x 50 µl rxns

1725080
Pkg of 1, kit includes 5 ml (1 x 5 ml) of cell lysis buffer, 100 µl of proteinase K solution, and 100 µl of DNase solution, for 100 x 50 µl reactions

1725080 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5080_1401909162.xml 1725080 06/04/14 10:53 AM en_US true internet/product_SKU 2018-02-27 06:49:38 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5080_1401909162.xml /default/main/bio-rad/WORKAREA/default jcrucywt 2017-11-14 07:07:36 true jcrucywt templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5080_1401909162.xml LSR N /default/main/bio-rad en 06/05/24 12:11 AM 1519437130204 2018-02-27 06:49:38 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5080_1401909162.xml 1725080-singleshot-cell-lysis-kit-100-x-50-ul-rxns 1 1725080 SingleShotCellLysisKit SingleShot™ Cell Lysis Kit, 100 x 50 µl rxns Pkg of 1, kit includes 5 ml <span class="forcebreaks"> (1 x 5 ml)</span> of cell lysis buffer, 100 µl of proteinase K solution, and 100 µl of DNase solution, for 100 x 50 µl reactions Pkg of 1, kit includes 5 ml <span class="forcebreaks"> (1 x 5 ml)</span> of cell lysis buffer, 100 µl of proteinase K solution, and 100 µl of DNase solution, for 100 x 50 µl reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/single-shot-cell-lysis-kit-172-5080-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/single-shot-cell-lysis-kit-172-5080.jpg SingleShot&trade; Cell Lysis Kit preview Karen Moss SingleShot™ Cell Lysis Kit SingleShot™ Cell Lysis Kit, 100 x 50 µl reactions 1725080, singleshot, cell, lysis, 100x50, reactions, break, down, rna, cdna, one-step, rt-qpcr, reverse, transcription, quantitative, pcr, detection, amplification 06/04/14 10:53 AM 06/05/24 12:11 AM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use the <strong>SingleShot Cell Lysis Kit</strong> to rapidly generate cell lysates that are suitable for RT-qPCR analysis without RNA purification.</p> <p>Kit works on 10 to 100,000 cells in 20 minutes while providing the following key benefits:</p> <ul> <li>Cell lysate from 10 to 100,000 cells ready in 20 minutes</li> <li>Complete removal of genomic DNA while <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/potent-rnase-inhibitor-preserves-rna-integrity.html');">preserving RNA integrity</a></li> <li>Optimal accuracy and <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/optimal-efficiency-across-expression-ranges.html');">linear dynamic range</a> of qPCR data across genes</li> <li>Results comparable to using purified RNA, including dynamic range</li> <li>Validated with PrimePCR&trade; Assays and Panels (visit <a href="http://www.bio-rad.com/primepcr">www.bio-rad.com/primepcr</a>)</li> <li>SingleShot RNA Control included to ensure optimal cell and lysate inputs</li> </ul> <h2 class="descHeader">Optimized Sample Input</h2> <p>The SingleShot Cell Lysis Kit maximizes cell lysate and cDNA inputs in RT-qPCR reactions:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td>Maximum cell lysate into RT reaction</td> <td>45% of total volume</td> </tr> <tr class="pd_colorbackground"> <td>Maximum undiluted cDNA into qPCR reaction</td> <td>20% of total volume</td> </tr> <tr> <td>Maximum cell lysate into one-step RT-qPCR reaction</td> <td>20% of total volume</td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Kit Contents</h2> <table class="pd_table" style="width: 500px;" border="0"> <tbody> <tr> <td align="left" valign="top"> <p><strong>SingleShot Cell Lysis Reagents</strong><br /> SingleShot Cell Lysis Buffer&nbsp;<br /> SingleShot Proteinase K Solution&nbsp;<br /> SingleShot DNase Solution</p> </td> <td align="left" valign="top"> <p><strong>100 x 50 &micro;l reactions</strong> <br /> 5 ml (1 x 5 ml vial)<br /> 100 &micro;l (1 x 1 ml vial)<br /> 100 &micro;l (1 x 1 ml vial)</p> </td> </tr> </tbody> </table> <p>Refer to the <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10042477.pdf" target="_blank" rel="noopener noreferrer">user manual</a> for details on reaction setup and troubleshooting tips.</p> <h2 class="descHeader">Cell Lines Validated for Use with the SingleShot Cell Lysis Kit</h2> <p>The SingleShot Cell Lysis RT-qPCR Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td><strong>Cell Line</strong></td> <td><strong>Property</strong></td> <td><strong> Species</strong></td> </tr> <tr class="pd_colorbackground"> <td>A549</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, lung carcinoma</td> </tr> <tr> <td>HeLa</td> <td>Adherent</td> <td><em>H. sapiens</em>, cervical adenocarcinoma</td> </tr> <tr class="pd_colorbackground"> <td>HepG2&nbsp;&nbsp;</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, liver carcinoma</td> </tr> <tr> <td>NIH/3T3</td> <td>Adherent</td> <td><em>M. musculus</em>, embryonic fibroblast</td> </tr> <tr class="pd_colorbackground"> <td>MCF-7</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, epithelial, adenocarcinoma</td> </tr> <tr> <td>NCI-H1975&nbsp;</td> <td>Adherent</td> <td><em>H. sapiens</em>, lung adenocarcinoma&nbsp;</td> </tr> <tr class="pd_colorbackground"> <td>K-562</td> <td>Suspension</td> <td><em>H. sapiens</em>, chronic myelogenous leukemia</td> </tr> <tr> <td>HEK 293</td> <td>Suspension</td> <td><em>H. sapiens</em>, kidney</td> </tr> <tr class="pd_colorbackground"> <td>Jurkat</td> <td>Suspension</td> <td><em>H. sapiens</em>, T lymphocyte</td> </tr> <tr> <td>PC-12&nbsp;</td> <td>Suspension</td> <td><em>R. norvegicus</em>, pheochromocytoma, adrenal</td> </tr> <tr class="pd_colorbackground"> <td>HepRG&nbsp;&nbsp;</td> <td>Primary-like</td> <td><em>H. sapiens</em>, hepatocytes</td> </tr> </tbody> </table> &nbsp; <h2 class="descHeader">RT-qPCR Step Reagents (Sold Separately)</h2> <p>Reverse Transcription</p> <ul> <li>iScript&trade; Advanced cDNA Synthesis Kit for RT-qPCR (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5038">172-5038</a>)</li> </ul> <p>Two-Step RT-qPCR Reagents&nbsp;&nbsp;</p> <ul> <li>SsoAdvanced&trade; Universal SYBR<sup>&reg;</sup> Green Supermix (<a href="/en-us/sku/172-5271-ssoadvanced-universal-sybr-green-supermix">172-5271</a>)</li> <li>SsoAdvanced&trade; Universal Probes Supermix (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5281">172-5281</a>)</li> </ul> <p>One-Step RT-qPCR Reagents</p> <ul> <ul> <li>iTaq&trade; Universal SYBR<sup>&reg;</sup> Green One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5151">172-5151</a>)</li> <li>iTaq&trade; Universal Probes One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5141">172-5141</a>)</li> </ul> </ul> <br /> <ul> <li><a href="http://www.bio-rad.com/evportal/evolutionPortal.portal?_nfpb=true&amp;_pageLabel=oligosearch_landing&amp;mode=full&amp;vertical=LSR&amp;filters=CTRL&amp;ControlSymbol=RT">PrimePCR&trade; Reverse Transcription Control Assay</a><br /> Use of the optional PrimePCR Reverse Transcription Control Assay in a small pilot experiment enables the determination of the optimal cell count and lysate inputs. The control can be added at the beginning to monitor the lysis step and determine the optimal cell count, or during the qPCR step to monitor RT-qPCR inhibition.</li> </ul> <h2 class="descHeader">Related Products</h2> <p>The SingleShot Cell Lysis Kits belong to the family of <a href="/en-us/product/cell-lysis-rt-qpcr-kits">SingleShot&trade; Cell Lysis RT-qPCR Kits</a>:</p> <ul> <li><strong>SingleShot Cell Lysis One-Step RT-qPCR Kits</strong> &mdash; including SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5095">172-5095</a>) and SingleShot Probes One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5070">172-5070</a>)</li> <li><strong>SingleShot Cell Lysis RT-qPCR Kits</strong> &mdash; including SingleShot&trade; SYBR<sup>&reg;</sup> Green Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5085">172-5085</a>) and SingleShot Probes Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5090">172-5090</a>)</li> <li><strong>SingleShot Cell Lysis Kit</strong> &mdash; available for 100 reactions (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5080">172-5080</a>)</li> </ul> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#rNaseinhibitor').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/potent-rnase-inhibitor-preserves-rna-integrity.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#optimalEfficiency').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/optimal-efficiency-across-expression-ranges.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#cellLysates').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/cell-lysates-vs-purified-rna.html'); }); }); // ]]></script> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global singleshot-cell lysis-kit-172-5080-desc2.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/singleshot-cell lysis-kit-172-5080-desc2.html Storage at –20°C 2199 <strong>12 months:</strong><br />Lysis buffer<br /> DNase solution<br /> Proteinase K solution 1 0 Storage at 4°C 2805 <strong>6 months:</strong><br />Lysis buffer 1 0 1725038 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5038_1403297461.xml 1725271 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5271_1361992538.xml 1725281 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5281_1362100128.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725080&CartQuote=QT&quantity= false true false $494.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725080&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725080&quantity= 1725080
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SingleShot™ Cell Lysis Kit, 500 x 50 µl rxns

1725081
Pkg of 1, kit includes 25 ml (5 x 5 ml) of cell lysis buffer, 500 µl of proteinase K solution, and 500 µl of DNase solution, for 500 x 50 µl reactions

1725081 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5081_1401916730.xml 1725081 06/04/14 02:06 PM en_US true internet/product_SKU 2018-02-27 06:49:39 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5081_1401916730.xml /default/main/bio-rad/WORKAREA/default jcrucyxx 2017-11-14 07:07:36 true jcrucyxx templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5081_1401916730.xml LSR N /default/main/bio-rad en 06/04/24 02:18 PM 1519353027543 2018-02-27 06:49:39 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5081_1401916730.xml 1725081-singleshot-cell-lysis-kit-500-x-50-ul-rxns 1 1725081 SingleShotCellLysisKit SingleShot™ Cell Lysis Kit, 500 x 50 µl rxns Pkg of 1, kit includes 25 ml <span class="forcebreaks"> (5 x 5 ml)</span> of cell lysis buffer, 500 µl of proteinase K solution, and 500 µl of DNase solution, for 500 x 50 µl reactions Pkg of 1, kit includes 25 ml <span class="forcebreaks"> (5 x 5 ml)</span> of cell lysis buffer, 500 µl of proteinase K solution, and 500 µl of DNase solution, for 500 x 50 µl reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/single-shot-cell-lysis-kit-172-5081-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/single-shot-cell-lysis-kit-172-5081.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/single-shot-cell-lysis-kit-172-5081.jpg preview Karen Moss SingleShot&trade; Cell Lysis Kit SingleShot™ Cell Lysis Kit, 500 x 50 µl reactions 1725081, singleshot, cell, lysis, 500x50, reactions, break, down, rna, cdna, one-step, rt-qpcr, reverse, transcription, quantitative, pcr, detection, amplification 06/04/14 02:06 PM 06/04/24 02:18 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use the<strong> SingleShot Cell Lysis Kit</strong> to rapidly generate cell lysates that are suitable for RT-qPCR analysis without RNA purification.</p> <p>Kit works on 10 to 100,000 cells in 20 minutes while providing the following key benefits:</p> <ul> <li>Cell lysate from 10 to 100,000 cells ready in 20 minutes</li> <li>Complete removal of genomic DNA while <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/potent-rnase-inhibitor-preserves-rna-integrity.html');">preserving RNA integrity</a></li> <li>Optimal accuracy and <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/optimal-efficiency-across-expression-ranges.html');">linear dynamic range</a> of qPCR data across genes</li> <li>Results comparable to using purified RNA, including dynamic range</li> <li>Validated with PrimePCR&trade; Assays and Panels (visit <a href="http://www.bio-rad.com/primepcr">www.bio-rad.com/primepcr</a>)</li> <li>SingleShot RNA Control included to ensure optimal cell and lysate inputs</li> </ul> <h2 class="descHeader">Optimized Sample Input</h2> <p>The SingleShot Cell Lysis Kit maximizes cell lysate and cDNA inputs in RT-qPCR reactions:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td>Maximum cell lysate into RT reaction</td> <td>45% of total volume</td> </tr> <tr class="pd_colorbackground"> <td>Maximum undiluted cDNA into qPCR reaction</td> <td>20% of total volume</td> </tr> <tr> <td>Maximum cell lysate into one-step RT-qPCR reaction</td> <td>20% of total volume</td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Kit Contents</h2> <table class="pd_table" style="width: 500px;" border="0"> <tbody> <tr> <td align="left" valign="top"> <p><strong>SingleShot Cell Lysis Reagents</strong><br /> SingleShot Cell Lysis Buffer&nbsp;<br /> SingleShot Proteinase K Solution&nbsp;<br /> SingleShot DNase Solution</p> </td> <td align="left" valign="top"> <p><strong>500 x 50 &micro;l reactions</strong> <br /> 25 ml (5 x 5 ml vial)<br /> 500 &micro;l (1 x 1 ml vial)<br /> 500 &micro;l (1 x 1 ml vial)</p> </td> </tr> </tbody> </table> <p>Refer to the <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10042477.pdf" target="_blank" rel="noopener noreferrer">user manual</a> for details on reaction setup and troubleshooting tips.</p> <h2 class="descHeader">Cell Lines Validated for Use with the SingleShot&trade; Cell Lysis Kit</h2> <p>The SingleShot&trade; Cell Lysis Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td><strong>Cell Line</strong></td> <td><strong>Property</strong></td> <td><strong> Species</strong></td> </tr> <tr class="pd_colorbackground"> <td>A549</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, lung carcinoma</td> </tr> <tr> <td>HeLa</td> <td>Adherent</td> <td><em>H. sapiens</em>, cervical adenocarcinoma</td> </tr> <tr class="pd_colorbackground"> <td>HepG2&nbsp;&nbsp;</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, liver carcinoma</td> </tr> <tr> <td>NIH/3T3</td> <td>Adherent</td> <td><em>M. musculus</em>, embryonic fibroblast</td> </tr> <tr class="pd_colorbackground"> <td>MCF-7</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, epithelial, adenocarcinoma</td> </tr> <tr> <td>NCI-H1975&nbsp;</td> <td>Adherent</td> <td><em>H. sapiens</em>, lung adenocarcinoma&nbsp;</td> </tr> <tr class="pd_colorbackground"> <td>K-562</td> <td>Suspension</td> <td><em>H. sapiens</em>, chronic myelogenous leukemia</td> </tr> <tr> <td>HEK 293</td> <td>Suspension</td> <td><em>H. sapiens</em>, kidney</td> </tr> <tr class="pd_colorbackground"> <td>Jurkat</td> <td>Suspension</td> <td><em>H. sapiens</em>, T lymphocyte</td> </tr> <tr> <td>PC-12&nbsp;</td> <td>Suspension</td> <td><em>R. norvegicus</em>, pheochromocytoma, adrenal</td> </tr> <tr class="pd_colorbackground"> <td>HepRG&nbsp;&nbsp;</td> <td>Primary-like</td> <td><em>H. sapiens</em>, hepatocytes</td> </tr> </tbody> </table> &nbsp; <h2 class="descHeader">RT-qPCR Step Reagents (Sold Separately)</h2> <p>Reverse Transcription</p> <ul> <li>iScript&trade; Advanced cDNA Synthesis Kit for RT-qPCR (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5038">172-5038</a>)</li> </ul> <p>Two-Step RT-qPCR Reagents&nbsp;&nbsp;</p> <ul> <li>SsoAdvanced&trade; Universal SYBR<sup>&reg;</sup> Green Supermix (<a href="/en-us/sku/172-5271-ssoadvanced-universal-sybr-green-supermix">172-5271</a>)</li> <li>SsoAdvanced&trade; Universal Probes Supermix (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5281">172-5281</a>)</li> </ul> <p>One-Step RT-qPCR Reagents</p> <ul> <ul> <li>iTaq&trade; Universal SYBR<sup>&reg;</sup> Green One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5151">172-5151</a>)</li> <li>iTaq&trade; Universal Probes One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5141">172-5141)</a></li> </ul> </ul> <br /> <ul> <li><a href="http://www.bio-rad.com/evportal/evolutionPortal.portal?_nfpb=true&amp;_pageLabel=oligosearch_landing&amp;mode=full&amp;vertical=LSR&amp;filters=CTRL&amp;ControlSymbol=RT">PrimePCR&trade; Reverse Transcription Control Assay</a></li> Use of the optional PrimePCR Reverse Transcription Control Assay in a small pilot experiment enables the determination of the optimal cell count and lysate inputs. The control can be added at the beginning to monitor the lysis step and determine the optimal cell count, or during the qPCR step to monitor RT-qPCR inhibition.</ul> <h2 class="descHeader">Related Products</h2> <p>The SingleShot Cell Lysis Kits belong to the family of <a href="/en-us/product/cell-lysis-rt-qpcr-kits">SingleShot&trade; Cell Lysis RT-qPCR Kits</a>:</p> <ul> <li><strong>SingleShot Cell Lysis One-Step RT-qPCR Kits</strong> &mdash; including SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5095">172-5095</a>) and SingleShot Probes One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5070">172-5070</a>)</li> <li><strong>SingleShot Cell Lysis RT-qPCR Kits</strong> &mdash; including SingleShot&trade; SYBR<sup>&reg;</sup> Green Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5085">172-5085</a>) and SingleShot Probes Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5090">172-5090</a>)</li> <li><strong>SingleShot Cell Lysis Kit</strong> &mdash; available for 100 reactions (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5080">172-5080</a>)</li> </ul> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#rNaseinhibitor').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/potent-rnase-inhibitor-preserves-rna-integrity.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#optimalEfficiency').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/optimal-efficiency-across-expression-ranges.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#cellLysates').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/cell-lysates-vs-purified-rna.html'); }); }); // ]]></script> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global singleshot-cell-lysis-kit-172-5081-desc1.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/singleshot-cell-lysis-kit-172-5081-desc1.html Storage at –20°C 2199 <strong>12 months:</strong><br /> Lysis buffer<br /> DNase solution<br /> Proteinase K solution 1 0 Storage at 4°C 2757 <strong>6 months:</strong><br />Lysis buffer 1 0 1725038 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5038_1403297461.xml 1725271 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5271_1361992538.xml 1725281 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5281_1362100128.xml false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725081&CartQuote=QT&quantity= false true false $2,189.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725081&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725081&quantity= 1725081
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SingleShot™ SYBR® Green Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions

1725085
Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal SYBR® Green Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions

1725085 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5085_1401922795.xml 1725085 06/04/14 02:40 PM en_US true internet/product_SKU 2018-02-27 06:49:38 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5085_1401922795.xml /default/main/bio-rad/WORKAREA/default jcihuc4k 2017-11-14 07:07:36 true jcihuc4k templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5085_1401922795.xml LSR N /default/main/bio-rad en 06/04/24 03:59 PM 1519706398781 2018-02-27 06:49:38 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5085_1401922795.xml 1725085-singleshot-sybr-green-kit-for-cell-lysis-rt-qpcr-100-x-50-ul-reactions 1 1725085 SingleShotSYBRGreenKit SingleShot™ SYBR<sup>®</sup> Green Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript&trade; Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced&trade; Universal SYBR<sup>&reg;</sup> Green Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript&trade; Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced&trade; Universal SYBR<sup>&reg;</sup> Green Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/singleshot-sybr-green-kit-172-5085-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/singleshot-sybr-green-kit-172-5085.jpg preview Karen Moss SingleShot™ SYBR® Green Cell Lysis and RT-qPCR Kit SingleShot™ SYBR<sup>®</sup> Green Cell Lysis and RT-qPCR Kit, 100 x 50 µl reactions 1725085, singleshot, sybr, green, cell, lysis, reagents, iscript, cdna, synthesis, supermix, one, step, rt-qpcr, reverse, transcription, quantitative, pcr, detection, amplification 06/04/14 02:40 PM 06/04/24 03:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use the <strong>SingleShot&trade; SYBR<sup>&reg;</sup> Green Kit</strong> to obtain high-quality RT-qPCR data with SYBR<sup>&reg;</sup> Green chemistry directly from cell culture lysates.</p> <p>Two-step workflow provides results less than 2 hours after cell lysis. All RT-qPCR reagents and RNA control included.</p> <p>Benefits include:</p> <ul> <li>Cell lysate from 10 to 100,000 cells ready in 20 minutes</li> <li>Complete <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/genomic-dna-removal-from-cell-lysates.html');">removal of genomic DNA</a> while <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/potent-rnase-inhibitor-preserves-rna-integrity.html');">preserving RNA integrity</a></li> <li><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/reproducibility-of-technical-lysate.html');">Optimal accuracy</a> and linear dynamic range of qPCR data across genes</li> <li>Results comparable to using purified RNA, including dynamic range</li> <li>Validated with PrimePCR&trade; Assays and Panels (visit <a href="http://www.bio-rad.com/primepcr">www.bio-rad.com/primepcr</a>)</li> <li>SingleShot RNA Control ensures optimal cell and lysate inputs</li> </ul> <h2 class="descHeader">Optimized Sample Input</h2> <p>The SingleShot&trade; SYBR<sup>&reg;</sup> Green Kit maximizes cell lysate and cDNA inputs in a two-step RT-qPCR reaction:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td>Maximum cell lysate into RT reaction</td> <td>45% of total volume</td> </tr> <tr class="pd_colorbackground"> <td>Maximum undiluted cDNA into qPCR reaction</td> <td>20% of total volume</td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Kit Contents</h2> <table class="pd_table" style="width: 500px;" border="0"> <tbody> <tr> <td width="310" align="left" valign="top"> <p><strong>SingleShot Cell Lysis Reagents</strong><br /> SingleShot Cell Lysis Buffer <br /> SingleShot Proteinase K Solution <br /> SingleShot DNase Solution</p> </td> <td width="190" align="left" valign="top"> <p><strong>100 x 50 &mu;l reactions</strong><br /> 5 ml (1 x 5 ml vial)<br />100 &mu;l (1 x 1 ml vial)<br />100 &mu;l (1 x 1 ml vial)</p> </td> </tr> </tbody> </table> <table class="pd_table" style="width: 500px;" border="0"> <tbody> <tr> <td width="310" align="left" valign="top"> <p><strong>cDNA Synthesis Kit</strong><br /> 5x iScript Advanced Reaction Mix<br /> iScript Advanced Reverse Transcriptase<br /> Nuclease-Free Water</p> </td> <td width="190" align="left" valign="top"> <p><strong>100 reactions</strong><br /> 400 &mu;l (1 x 1 ml vial)<br /> 100 &mu;l (1 x 1 ml vial)<br /> 1 ml (1 x 1 ml vial)</p> </td> </tr> </tbody> </table> <table class="pd_table" style="width: 500px;" border="0"> <tbody> <tr> <td width="310" align="left" valign="top"> <p><strong>qPCR Supermix</strong><br /> SsoAdvanced Universal SYBR<sup>&reg;</sup> Green Supermix</p> </td> <td width="190" align="left" valign="top"> <p><strong> 500 reactions</strong><br /> 5 ml (5 x 1 ml vials)</p> </td> </tr> </tbody> </table> <table class="pd_table" style="width: 500px;" border="0"> <tbody> <tr> <td width="310" align="left" valign="top"> <p><strong>SingleShot RNA Control </strong><br /> SingleShot RNA Control Template <br /> SingleShot&trade; SYBR<sup>&reg;</sup> Green qPCR Control Assay</p> </td> <td width="190" align="left" valign="top"> <p><strong> 200 reactions</strong><br /> Lyophilized (200 reactions)<br />200 &mu;l (1 x 1 ml vial)</p> </td> </tr> </tbody> </table> <p>Refer to the <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10042474.pdf" target="_blank" rel="noopener noreferrer">user manual</a> for details on reaction setup and troubleshooting tips.</p> <h2 class="descHeader">Cell Lines Validated for Use with the SingleShot&trade; SYBR<sup>&reg;</sup> Green Kit</h2> <p>The SingleShot&trade; SYBR<sup>&reg;</sup> Green Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td width="75"><strong>Cell Line</strong></td> <td width="88"><strong>Property</strong></td> <td width="323"><strong> Species</strong></td> </tr> <tr class="pd_colorbackground"> <td>A549</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, lung carcinoma</td> </tr> <tr> <td>HeLa</td> <td>Adherent</td> <td><em>H. sapiens</em>, cervical adenocarcinoma</td> </tr> <tr class="pd_colorbackground"> <td>HepG2&nbsp;&nbsp;</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, liver carcinoma</td> </tr> <tr> <td>NIH/3T3</td> <td>Adherent</td> <td><em>M. musculus</em>, embryonic fibroblast</td> </tr> <tr class="pd_colorbackground"> <td>MCF-7</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, epithelial, adenocarcinoma</td> </tr> <tr> <td>NCI-H1975</td> <td>Adherent</td> <td><em>H. sapiens</em>, lung adenocarcinoma&nbsp;</td> </tr> <tr class="pd_colorbackground"> <td>K-562</td> <td>Suspension</td> <td><em>H. sapiens</em>, chronic myelogenous leukemia</td> </tr> <tr> <td>HEK 293</td> <td>Suspension</td> <td><em>H. sapiens</em>, kidney</td> </tr> <tr class="pd_colorbackground"> <td>Jurkat</td> <td>Suspension</td> <td><em>H. sapiens</em>, T lymphocyte</td> </tr> <tr> <td>PC-12&nbsp;</td> <td>Suspension</td> <td><em>R. norvegicus</em>, pheochromocytoma, adrenal</td> </tr> <tr class="pd_colorbackground"> <td>HepRG&nbsp;&nbsp;</td> <td>Primary-like</td> <td><em>H. sapiens</em>, hepatocytes</td> </tr> </tbody> </table> &nbsp; <h2 class="descHeader">Related Products</h2> <p>The SingleShot&trade; SYBR<sup>&reg;</sup> Green Kit belongs to the family of <a href="/en-us/product/cell-lysis-rt-qpcr-kits">SingleShot&trade; Cell Lysis RT-qPCR Kits</a>:</p> <ul> <li><strong>SingleShot Cell Lysis One-Step RT-qPCR Kits</strong> &mdash; including SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5095">172-5095</a>) and SingleShot Probes One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5070">172-5070</a>)</li> <li><strong>SingleShot Cell Lysis RT-qPCR Kits</strong> &mdash; including SingleShot&trade; SYBR<sup>&reg;</sup> Green Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5085">172-5085</a>) and SingleShot Probes Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5090">172-5090</a>)</li> <li><strong>SingleShot Cell Lysis Kit</strong> &mdash; available for 100 reactions (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5080">172-5080</a>)</li> </ul> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#optimalEfficiency').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/cell-lysates-vs-purified-rna.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#genomicDNA').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/potent-rnase-inhibitor-preserves-rna-integrity.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#genomicDNA').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/genomic-dna-removal-from-cell-lysates.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#reproducibility').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/reproducibility-of-technical-lysate.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#optimalEfficiency').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/optimal-efficiency-across-expression-ranges.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#optimalEfficiency').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sybr-green-isolated-rna-comparison.html'); }); }); // ]]></script> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global singleshot-sybr-green-kit-172-5085-desc1.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/singleshot-sybr-green-kit-172-5085-desc1.html Storage at –20°C 2199 <strong>12 months:</strong><br /> Lysis buffer, DNase, and proteinase K solution<br /> iScript Advanced cDNA Synthesis Kit<br /> SsoAdvanced&trade; Universal SYBR<sup>&reg;</sup> Green Supermix <br /> SingleShot RNA Control Template<br /> SingleShot&trade; SYBR<sup>&reg;</sup> Green qPCR Control Assay 1 0 Storage at 4°C 2805 <strong>6 months:</strong><br />Lysis buffer<br /><br /> <strong>Up to 3 months:</strong><br />SsoAdvanced&trade; Universal SYBR<sup>&reg;</sup> Green Supermix 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725085&CartQuote=QT&quantity= false true false $1,205.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725085&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725085&quantity= 1725085
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SingleShot™ Probes Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions

1725090
Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript™ Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced™ Universal Probes Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions

1725090 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5090_1401931070.xml 1725090 06/04/14 05:42 PM en_US true internet/product_SKU 2018-02-27 06:49:38 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5090_1401931070.xml /default/main/bio-rad/WORKAREA/default jcrucywx 2017-11-14 07:07:36 true jcrucywx templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5090_1401931070.xml LSR N /default/main/bio-rad en 06/04/24 06:16 PM 1519353027631 2018-02-27 06:49:38 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5090_1401931070.xml 1725090-singleshot-probes-kit-for-cell-lysis-rt-qpcr-100-x-50-ul-reactions 1 1725090 SingleShotProbesKit SingleShot™ Probes Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript&trade; Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced&trade; Universal Probes Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iScript&trade; Advanced cDNA Synthesis Kit for RT-qPCR, SsoAdvanced&trade; Universal Probes Supermix, and the SingleShot RNA Control, for 100 x 50 µl reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/single-shot-probes-kit-172-5090-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/single-shot-probes-kit-172-5090.jpg preview Karen Moss SingleShot&trade; Probes Kit SingleShot™ Probes Cell Lysis and RT-qPCR Analysis Kit, 100 x 50 µl reactions 1725090, singleshot, probes, cell, lysis, reagents, iscript, cdna, synthesis, probe, one-step, one, step, rt-qpcr, reverse, transcription, quantitative, pcr, detection, amplification 06/04/14 05:42 PM 06/04/24 06:16 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use the <strong>SingleShot Probes Kit</strong> to obtain high-quality RT-qPCR data with probe chemistry directly from cell culture lysates.</p> <p>Two-step workflow provides results in less than 2 hours after cell lysis. All RT-qPCR reagents and RNA control included.</p> <p>Benefits include:</p> <ul> <li>Cell lysate from 10 to 100,000 cells ready in 20 minutes</li> <li>Complete removal of genomic DNA while <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/potent-rnase-inhibitor-preserves-rna-integrity.html');">preserving RNA integrity</a></li> <li>Optimal accuracy and linear dynamic range of qPCR data across genes</li> <li>Results comparable to using purified RNA, including dynamic range</li> <li>Validated with PrimePCR&trade; Assays and Panels (visit <a href="http://www.bio-rad.com/primepcr">www.bio-rad.com/primepcr</a>)</li> <li>SingleShot RNA Control ensures optimal cell and lysate inputs</li> </ul> <h2 class="descHeader">Optimized Sample Input</h2> <p>The SingleShot Probes Kit maximizes cell lysate and cDNA inputs in a two-step RT-qPCR reaction:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td>Maximum cell lysate into RT reaction</td> <td>45% of total volume</td> </tr> <tr class="pd_colorbackground"> <td>Maximum undiluted cDNA into qPCR reaction</td> <td>20% of total volume</td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Kit Contents</h2> <table class="pd_table" style="width: 500px;" border="0"> <tbody> <tr> <td width="310" align="left" valign="top"> <p><strong>SingleShot Cell Lysis Reagents</strong><br /> SingleShot Cell Lysis Buffer <br /> SingleShot Proteinase K Solution <br /> SingleShot DNase Solution</p> </td> <td width="190" align="left" valign="top"> <p><strong>100 x 50 &mu;l reactions</strong><br /> 5 ml (1 x 5 ml vial)<br />100 &mu;l (1 x 1 ml vial)<br />100 &mu;l (1 x 1 ml vial)</p> </td> </tr> </tbody> </table> <table class="pd_table" style="width: 500px;" border="0"> <tbody> <tr> <td width="310" align="left" valign="top"> <p><strong>cDNA Synthesis Kit</strong><br /> 5x iScript Advanced Reaction Mix<br /> iScript Advanced Reverse Transcriptase<br /> Nuclease-Free Water</p> </td> <td width="190" align="left" valign="top"> <p><strong>100 reactions</strong><br /> 400 &mu;l (1 x 1 ml vial)<br /> 100 &mu;l (1 x 1 ml vial)<br /> 1 ml (1 x 1 ml vial)</p> </td> </tr> </tbody> </table> <table class="pd_table" style="width: 500px;" border="0"> <tbody> <tr> <td width="310" align="left" valign="top"> <p><strong>qPCR Supermix</strong><br /> SsoAdvanced Universal Probes Supermix</p> </td> <td width="190" align="left" valign="top"> <p><strong> 500 reactions</strong><br /> 5 ml (5 x 1 ml vials)</p> </td> </tr> </tbody> </table> <table class="pd_table" style="width: 500px;" border="0"> <tbody> <tr> <td width="310" align="left" valign="top"> <p><strong>SingleShot RNA Control </strong><br /> SingleShot RNA Control Template <br /> SingleShot Probes qPCR Control Assay</p> </td> <td width="190" align="left" valign="top"> <p><strong> 200 reactions</strong><br /> Lyophilized (200 reactions)<br />200 &mu;l (1 x 1 ml vial)</p> </td> </tr> </tbody> </table> <p>Refer to the <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10042473.pdf" target="_blank" rel="noopener noreferrer">user manual</a> for details on reaction setup and troubleshooting tips.</p> <h2 class="descHeader">Cell Lines Validated for Use with the SingleShot Probes Kit</h2> <p>The SingleShot Probes Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td width="75"><strong>Cell Line</strong></td> <td width="88"><strong>Property</strong></td> <td width="323"><strong> Species</strong></td> </tr> <tr class="pd_colorbackground"> <td>A549</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, lung carcinoma</td> </tr> <tr> <td>HeLa</td> <td>Adherent</td> <td><em>H. sapiens</em>, cervical adenocarcinoma</td> </tr> <tr class="pd_colorbackground"> <td>HepG2&nbsp;&nbsp;</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, liver carcinoma</td> </tr> <tr> <td>NIH/3T3</td> <td>Adherent</td> <td><em>M. musculus</em>, embryonic fibroblast</td> </tr> <tr class="pd_colorbackground"> <td>MCF-7</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, epithelial, adenocarcinoma</td> </tr> <tr> <td>NCI-H1975&nbsp;</td> <td>Adherent</td> <td><em>H. sapiens</em>, lung adenocarcinoma&nbsp;</td> </tr> <tr class="pd_colorbackground"> <td>K-562</td> <td>Suspension</td> <td><em>H. sapiens</em>, chronic myelogenous leukemia</td> </tr> <tr> <td>HEK 293</td> <td>Suspension</td> <td><em>H. sapiens</em>, kidney</td> </tr> <tr class="pd_colorbackground"> <td>Jurkat</td> <td>Suspension</td> <td><em>H. sapiens</em>, T lymphocyte</td> </tr> <tr> <td>PC-12&nbsp;</td> <td>Suspension</td> <td><em>R. norvegicus</em>, pheochromocytoma, adrenal</td> </tr> <tr class="pd_colorbackground"> <td>HepRG&nbsp;&nbsp;</td> <td>Primary-like</td> <td><em>H. sapiens</em>, hepatocytes</td> </tr> </tbody> </table> &nbsp; <h2 class="descHeader">Related Products</h2> <p>The SingleShot Probes Kit belongs to the family of <a href="/en-us/product/cell-lysis-rt-qpcr-kits">SingleShot&trade; Cell Lysis RT-qPCR Kits</a>:</p> <ul> <li><strong>SingleShot Cell Lysis One-Step RT-qPCR Kits</strong> &mdash; including SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5095">172-5095</a>) and SingleShot Probes One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5070">172-5070</a>)</li> <li><strong>SingleShot Cell Lysis RT-qPCR Kits</strong> &mdash; including SingleShot&trade; SYBR<sup>&reg;</sup> Green Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5085">172-5085</a>) and SingleShot Probes Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5090">172-5090</a>)</li> <li><strong>SingleShot Cell Lysis Kit</strong> &mdash; available for 100 reactions (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5080">172-5080</a>)</li> </ul> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#genomicDNA').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/genomic-dna-removal-from-cell-lysates.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#reproducibility').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/reproducibility-of-technical-lysate.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#optimalEfficiency').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/optimal-efficiency-across-expression-ranges.html'); }); }); // ]]></script> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global singleshot-probes-kit-172-5090-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/singleshot-probes-kit-172-5090-desc.html Storage at –20°C 2199 <strong>12 months:</strong><br /> Lysis buffer, DNase, and proteinase K solution<br /> iScript Advanced cDNA Synthesis Kit<br /> SsoAdvanced Universal Probes Supermix <br /> SingleShot RNA Control Template<br /> SingleShot Probes qPCR Control Assay 1 0 Storage at 4°C 2805 <strong>6 months:</strong><br />Lysis buffer<br /><br /><strong>Up to 3 months:</strong><br /> SsoAdvanced Universal Probes Supermix 1 0 false false false 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SingleShot™ SYBR® Green One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions

1725095
Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iTaq™ Universal SYBR® Green One-Step Kit, and the SingleShot RNA Control, for 100 x 50 µl reactions

1725095 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5095_1401934097.xml 1725095 06/04/14 06:51 PM en_US true internet/product_SKU 2018-02-27 06:49:40 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5095_1401934097.xml /default/main/bio-rad/WORKAREA/default jcrucyx2 2017-11-14 07:07:36 true jcrucyx2 templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5095_1401934097.xml LSR N /default/main/bio-rad en 06/04/24 07:07 PM 1519353027675 2018-02-27 06:49:40 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5095_1401934097.xml 1725095-singleshot-sybr-green-one-step-kit-for-cell-lysis-rt-qpcr-100-x-50-ul-reactions 1 1725095 SingleShotSYBRGreenOne-StepKit SingleShot™ SYBR<sup>®</sup> Green One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iTaq&trade; Universal SYBR<sup>&reg;</sup> Green One-Step Kit, and the SingleShot RNA Control, for 100 x 50 µl reactions Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iTaq&trade; Universal SYBR<sup>&reg;</sup> Green One-Step Kit, and the SingleShot RNA Control, for 100 x 50 µl reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/singleshot-sybr-green-one-step-kit-172-5095-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/singleshot-sybr-green-one-step-kit-172-5095.jpg preview Karen Moss SingleShot&trade; SYBR&reg; Green One-Step Kit SingleShot™ SYBR<sup>®</sup> Green One-Step Cell Lysis and RT-qPCR Kit, 100 x 50 µl reactions 1725095, singleshot, sybr, green, one-step, itaq, universal, one, step, cell, lysis, rt-qpcr, reverse, transcription, quantitative, pcr, detection, amplification 06/04/14 06:51 PM 06/04/24 07:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use the <strong>SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit </strong>to obtain high-quality RT-qPCR data with SYBR<sup>&reg;</sup> Green chemistry directly from cell culture lysates in a one-step workflow 1 hour after cell lysis. All reagents and RNA control included.</p> <p>Benefits include:</p> <ul> <li>Cell lysate from 10 to 100,000 cells ready in 20 minutes</li> <li>Complete removal of genomic DNA while <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/potent-rnase-inhibitor-preserves-rna-integrity.html');">preserving RNA integrity</a></li> <li>Optimal accuracy and <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/optimal-efficiency-across-expression-ranges.html');">linear dynamic range</a> of qPCR data across genes</li> <li>Results comparable to using purified RNA, including <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sybr-green-isolated-rna-comparison.html');">dynamic range</a></li> <li>Validated with PrimePCR&trade; Assays and Panels (visit <a href="http://www.bio-rad.com/primepcr">www.bio-rad.com/primepcr</a>)</li> <li>SingleShot RNA Control ensures optimal cell and lysate inputs</li> </ul> <h2 class="descHeader">Optimized Sample Input</h2> <p>The SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit maximizes cell lysate input in a one-step RT-qPCR reaction:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td>Maximum cell lysate into RT-qPCR reaction</td> <td>20% of total volume</td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Kit Contents</h2> <table border="0" width="500"> <tbody> <tr> <td width="328" align="left" valign="top"><strong>SingleShot Cell Lysis Reagents</strong><br /> SingleShot Cell Lysis Buffer <br /> SingleShot Proteinase K Solution <br /> SingleShot DNase Solution</td> <td width="172" align="left" valign="top"><strong>100 x 50 &mu;l reactions</strong><br /> 5 ml (1 x 5 ml vial)<br /> 100 &mu;l (1 x 1 ml vial)<br /> 100 &mu;l (1 x 1 ml vial)</td> </tr> </tbody> </table> <br /> <table border="0" width="500"> <tbody> <tr> <td width="328" align="left" valign="top"><strong>iTaq&trade; Universal SYBR<sup>&reg;</sup> Green One-Step Kit </strong><br /> iScript&trade; Reverse Transcriptase<br /> 2x iTaq&trade; Universal SYBR<sup>&reg;</sup> Green One-Step Reaction Mix</td> <td width="172" align="left" valign="top"><strong> 100 reactions</strong><br /> 25 &mu;l (1 x 1 ml vial)<br /> 1 ml (1 x 1 ml vial)</td> </tr> </tbody> </table> <br /> <table border="0" width="500"> <tbody> <tr> <td width="328" align="left" valign="top"><strong>SingleShot RNA Control </strong><br /> SingleShot RNA Control Template <br />Singleshot&trade; SYBR<sup>&reg;</sup> Green qPCR Control Assay</td> <td width="172" align="left" valign="top"><strong> 200 reactions</strong><br /> Lyophilized (200 reactions)<br /> 200 &mu;l (1 x 1 ml vial)</td> </tr> </tbody> </table> &nbsp; <p>Refer to the <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10042475.pdf" target="_blank" rel="noopener noreferrer">user manual</a> for details on reaction setup and troubleshooting tips.</p> <h2 class="descHeader">Cell Lines Validated for Use with the SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit</h2> <p>The SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td><strong>Cell Line</strong></td> <td><strong>Property</strong></td> <td><strong> Species</strong></td> </tr> <tr class="pd_colorbackground"> <td>A549</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, lung carcinoma</td> </tr> <tr> <td>HeLa</td> <td>Adherent</td> <td><em>H. sapiens</em>, cervical adenocarcinoma</td> </tr> <tr class="pd_colorbackground"> <td>HepG2&nbsp;&nbsp;</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, liver carcinoma</td> </tr> <tr> <td>NIH/3T3</td> <td>Adherent</td> <td><em>M. musculus</em>, embryonic fibroblast</td> </tr> <tr class="pd_colorbackground"> <td>MCF-7</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, epithelial, adenocarcinoma</td> </tr> <tr> <td>NCI-H1975&nbsp;</td> <td>Adherent</td> <td><em>H. sapiens</em>, lung adenocarcinoma&nbsp;</td> </tr> <tr class="pd_colorbackground"> <td>K-562</td> <td>Suspension</td> <td><em>H. sapiens</em>, chronic myelogenous leukemia</td> </tr> <tr> <td>HEK 293</td> <td>Suspension</td> <td><em>H. sapiens</em>, kidney</td> </tr> <tr class="pd_colorbackground"> <td>Jurkat</td> <td>Suspension</td> <td><em>H. sapiens</em>, T lymphocyte</td> </tr> <tr> <td>PC-12&nbsp;</td> <td>Suspension</td> <td><em>R. norvegicus</em>, pheochromocytoma, adrenal</td> </tr> <tr class="pd_colorbackground"> <td>HepRG</td> <td>Primary-like</td> <td><em>H. sapiens</em>, hepatocytes</td> </tr> </tbody> </table> &nbsp; <h2 class="descHeader">Related Products</h2> <p>The SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit belongs to the family of <a href="/en-us/product/cell-lysis-rt-qpcr-kits">SingleShot&trade; Cell Lysis RT-pPCR Kits</a>:</p> <ul> <li><strong>SingleShot Cell Lysis One-Step RT-qPCR Kits</strong> &mdash; including SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5095">172-5095</a>) and SingleShot Probes One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5070">172-5070</a>)</li> <li><strong>SingleShot Cell Lysis RT-qPCR Kits</strong> &mdash; including SingleShot&trade; SYBR<sup>&reg;</sup> Green Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5085">172-5085</a>) and SingleShot Probes Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5090">172-5090</a>)</li> <li><strong>SingleShot Cell Lysis Kit</strong> &mdash; available for 100 reactions (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5080">172-5080</a>)</li> </ul> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#optimalEfficiency').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/cell-lysates-vs-purified-rna.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#genomicDNA').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/potent-rnase-inhibitor-preserves-rna-integrity.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#genomicDNA').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/genomic-dna-removal-from-cell-lysates.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#reproducibility').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/reproducibility-of-technical-lysate.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#optimalEfficiency').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/optimal-efficiency-across-expression-ranges.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#optimalEfficiency').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sybr-green-isolated-rna-comparison.html'); }); }); // ]]></script> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global singleshot-probes-one-step-kit-172-5095-desc1.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/singleshot-probes-one-step-kit-172-5095-desc1.html Storage at –20°C 2199 <strong>12 months:</strong><br /> Lysis buffer, DNase, and proteinase K solution<br /> iTaq&trade; Universal SYBR<sup>&reg;</sup> Green One-Step Reaction Mix<br /> iScript&trade; Reverse Transcriptase <br /> SingleShot RNA Control Template<br /> SingleShot&trade; SYBR<sup>&reg;</sup> Green qPCR Control Assay 1 0 Storage at 4°C 2805 <strong>6 months:</strong><br />Lysis buffer<br /><br /><strong>Up to 3 months:</strong><br />iTaq&trade; Universal SYBR<sup>&reg;</sup> Green One-Step Reaction Mix 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725095&CartQuote=QT&quantity= false true false $1,205.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725095&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725095&quantity= 1725095
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SingleShot™ Probes One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions

1725070
Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iTaq™ Universal Probes One-Step Kit, and the SingleShot RNA Control, for 100 x 50 µl reactions

1725070 /templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5070_1401932854.xml 1725070 06/04/14 06:26 PM en_US true internet/product_SKU 2018-02-27 06:49:41 /default/main/bio-rad/STAGING AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5070_1401932854.xml /default/main/bio-rad/WORKAREA/default jcrucyxr 2017-11-14 07:07:36 true jcrucyxr templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5070_1401932854.xml LSR N /default/main/bio-rad en 06/04/24 06:46 PM 1519353027452 2018-02-27 06:49:41 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSR/Amplification---PCR/PCR-Reagents/172-5070_1401932854.xml 1725070-singleshot-probes-one-step-kit-for-cell-lysis-rt-qpcr-100-x-50-ul-reactions 1 1725070 SingleShotProbesOne-StepKit SingleShot™ Probes One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iTaq&trade; Universal Probes One-Step Kit, and the SingleShot RNA Control, for 100 x 50 µl reactions Pkg of 1, kit includes SingleShot Cell Lysis Reagents, iTaq&trade; Universal Probes One-Step Kit, and the SingleShot RNA Control, for 100 x 50 µl reactions /webroot/web/images/lsr/products/amplification_pcr/sku_thumb/global/singleshot-probes-one-step-kit-172-5070-thumb.jpg /webroot/web/images/lsr/products/amplification_pcr/sku_view/global/singleshot-probes-one-step-kit-172-5070.jpg preview Karen Moss SingleShot&trade; Probes One-Step Kit SingleShot™ Probes One-Step Cell Lysis and RT-qPCR Analysis Kit, 100 x 50 µl reactions 1725070, singleshot, probes, one-step, rt-qpcr, quantitative, reverse, transcription, pcr, real-time, real, time, probe, one-step-rt-qpcr, cell, lysis, rna, cdna, detection, amplification, lysis, 06/04/14 06:26 PM 06/04/24 06:46 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Amplification---PCR/PCR-Reagents N 0 <p>Use the <strong>SingleShot Probes One-Step Kit</strong> to obtain high-quality RT-qPCR data with probe chemistry directly from cell culture lysates in a one-step workflow 1 hour after cell lysis. All reagents and RNA control included.</p> <p>Benefits include:</p> <ul> <li>Cell lysate from 10 to 100,000 cells ready in 20 minutes</li> <li>Complete removal of genomic DNA while <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/potent-rnase-inhibitor-preserves-rna-integrity.html');">preserving RNA integrity</a></li> <li>Optimal accuracy and linear dynamic range of qPCR data across genes</li> <li><a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/cell-lysates-vs-purified-rna.html');">Results comparable to using purified RNA</a>, including <a href="javascript:openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sybr-green-isolated-rna-comparison.html');">dynamic range</a></li> <li>Validated with PrimePCR&trade; Assays and Panels (visit <a href="http://www.bio-rad.com/primepcr">www.bio-rad.com/primepcr</a>)</li> <li>SingleShot RNA Control ensures optimal cell and lysate inputs</li> </ul> <h2 class="descHeader">Optimized Sample Input</h2> <p>The SingleShot Probes One-Step Kit maximizes cell lysate input in a one-step RT-qPCR reaction:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td>Maximum cell lysate into RT-qPCR reaction</td> <td>20% of total volume</td> </tr> </tbody> </table> <br /> <h2 class="descHeader">Kit Contents</h2> <table border="0" width="500"> <tbody> <tr> <td width="328" align="left" valign="top"><strong>SingleShot Cell Lysis Reagents</strong><br /> SingleShot Cell Lysis Buffer <br /> SingleShot Proteinase K Solution <br /> SingleShot DNase Solution</td> <td width="172" align="left" valign="top"><strong>100 x 50 &mu;l reactions</strong><br /> 5 ml (1 x 5 ml vial)<br /> 100 &mu;l (1 x 1 ml vial)<br /> 100 &mu;l (1 x 1 ml vial)</td> </tr> </tbody> </table> <br /> <table border="0" width="500"> <tbody> <tr> <td width="328" align="left" valign="top"><strong>iTaq Universal Probes One-Step Kit &nbsp;&nbsp;&nbsp;&nbsp;</strong><br /> iScript&trade; Reverse Transcriptase<br /> 2x iTaq Universal Probes One-Step Reaction Mix</td> <td width="172" align="left" valign="top"><strong> 100 reactions</strong><br /> 50 &mu;l (1 x 1 ml vial)<br /> 1 ml (1 x 1 ml vial)</td> </tr> </tbody> </table> <br /> <table border="0" width="500"> <tbody> <tr> <td width="328" align="left" valign="top"><strong>SingleShot RNA Control</strong><br /> SingleShot RNA Control Template <br /> SingleShot Probes qPCR Control Assay </td> <td width="172" align="left" valign="top"><strong> 200 reactions</strong><br /> Lyophilized (200 reactions)<br /> 200 &mu;l (1 x 1 ml vial)</td> </tr> </tbody> </table> <br /> <p>Refer to the <a class="no-target-style" href="/webroot/web/pdf/lsr/literature/10042476.pdf" target="_blank" rel="noopener noreferrer">user manual</a> for details on reaction setup and troubleshooting tips.</p> <h2 class="descHeader">Cell Lines Validated for Use with the SingleShot Probes One-Step Kit</h2> <p>The SingleShot Probes One-Step Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:</p> <table class="pd_table pd_gridlines" style="width: 500px;" border="0"> <tbody> <tr> <td><strong>Cell Line</strong></td> <td><strong>Property</strong></td> <td><strong> Species</strong></td> </tr> <tr class="pd_colorbackground"> <td>A549</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, lung carcinoma</td> </tr> <tr> <td>HeLa</td> <td>Adherent</td> <td><em>H. sapiens</em>, cervical adenocarcinoma</td> </tr> <tr class="pd_colorbackground"> <td>HepG2&nbsp;&nbsp;</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, liver carcinoma</td> </tr> <tr> <td>NIH/3T3</td> <td>Adherent</td> <td><em>M. musculus</em>, embryonic fibroblast</td> </tr> <tr class="pd_colorbackground"> <td>MCF-7</td> <td>Adherent&nbsp;</td> <td><em>H. sapiens</em>, epithelial, adenocarcinoma</td> </tr> <tr> <td>NCI-H1975&nbsp;</td> <td>Adherent</td> <td><em>H. sapiens</em>, lung adenocarcinoma&nbsp;</td> </tr> <tr class="pd_colorbackground"> <td>K-562</td> <td>Suspension</td> <td><em>H. sapiens</em>, chronic myelogenous leukemia</td> </tr> <tr> <td>HEK 293</td> <td>Suspension</td> <td><em>H. sapiens</em>, kidney</td> </tr> <tr class="pd_colorbackground"> <td>Jurkat</td> <td>Suspension</td> <td><em>H. sapiens</em>, T lymphocyte</td> </tr> <tr> <td>PC-12&nbsp;</td> <td>Suspension</td> <td><em>R. norvegicus</em>, pheochromocytoma, adrenal</td> </tr> <tr class="pd_colorbackground"> <td>HepRG</td> <td>Primary-like</td> <td><em>H. sapiens</em>, hepatocytes</td> </tr> </tbody> </table> &nbsp; <h2 class="descHeader">Related Products</h2> <p>The SingleShot Probes One-Step Kit belongs to the family of <a href="/en-us/product/cell-lysis-rt-qpcr-kits">SingleShot&trade; Cell Lysis RT-qPCR Kits</a>:</p> <ul> <li><strong>SingleShot Cell Lysis One-Step RT-qPCR Kits</strong> &mdash; including SingleShot&trade; SYBR<sup>&reg;</sup> Green One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5095">172-5095</a>) and SingleShot Probes One-Step Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5070">172-5070</a>)</li> <li><strong>SingleShot Cell Lysis RT-qPCR Kits</strong> &mdash; including SingleShot&trade; SYBR<sup>&reg;</sup> Green Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5085">172-5085</a>) and SingleShot Probes Kit (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5090">172-5090</a>)</li> <li><strong>SingleShot Cell Lysis Kit</strong> &mdash; available for 100 reactions (<a href="/evportal/destination/commerce/sku_detail?cmd=catProductDetail&amp;vertical=LSR&amp;productID=172-5080">172-5080</a>)</li> </ul> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#dynamicRange').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/large-dynamic-range-across-various-expressing-gene.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#sybrIsolated').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/sybr-green-isolated-rna-comparison.html'); }); }); // ]]></script> <script type="text/javascript">// <![CDATA[ $(document).ready(function(){ $('#probesIsolated').click(function(event){ event.preventDefault(); openAjaxOverlay('/webroot/web/html/lsr/products/amplification_pcr/product_overlay/global/probes-isolated-rna-comparison.html'); }); }); // ]]></script> /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global singleshot-sybr-green-one-step-kit-172-5070-desc.html /webroot/web/html/lsr/products/amplification_pcr/sku_view/description/global/singleshot-sybr-green-one-step-kit-172-5070-desc.html Storage at –20°C 2199 <strong>12 months:</strong><br /> Lysis buffer, DNase, and proteinase K solution<br /> iTaq Universal Probes One-Step Reaction Mix<br /> iScript&trade; Reverse Transcriptase<br /> SingleShot RNA Control Template<br /> SingleShot Probes qPCR Control Assay 1 0 Storage at 4°C 2805 <strong>6 months:</strong><br />Lysis buffer<br /><br /> <strong>Up to 3 months:</strong><br />iTaq Universal Probes One-Step Reaction Mix 1 0 false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725070&CartQuote=QT&quantity= false true false $1,205.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725070&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1725070&quantity= 1725070
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Number Description Options
10042474 User Manual, SingleShot™ SYBR® Green Kit, Rev A Click to download
10042473 User Manual, SingleShot™ Probes Kit, Rev A Click to download
6774 Parallel Analysis of RNA and Protein Expression Using SingleShot™ Cell Culture Lysates Application Note, Ver A Click to download
6776 Protocol for Parallel Analysis of RNA and Protein Expression Using SingleShot™ Cell Culture Lysates, Ver A Click to download
6777 Protocol for Single Cell Gene Expression Profiling by Multiplex RT-qPCR Click to download
10042475 User Manual, SingleShot™ SYBR® Green One-Step Kit, Rev A Click to download
10042476 User Manual, SingleShot™ Probes One-Step Kit, Rev A Click to download
10042477 User Manual, SingleShot™ Cell Lysis Kit, Rev A Click to download
6090 Amplification Reagents and Plastics Brochure Click to download [ Add to Cart (Free) ]
6629 High-Throughput Real-Time PCR Solutions Brochure, Ver B Click to download
6604 SingleShot™ Cell Lysis RT-qPCR Kits Application Note, Rev B Click to download [ Add to Cart (Free) ]
6572 Accurate and Reproducible RT-qPCR Gene Expression Analysis on Cell Culture Lysates Using the SingleShot™ SYBR® Green Kit, Rev A Click to download [ Add to Cart (Free) ]
6833 High Throughput Single Cell Gene Expression Profiling by Multiplex qPCR, Ver A Click to download

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