C. elegans Behavior Kit

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C elegans Behavior Kit

Overview

Lab Preparation Checklist
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
Store at –20°C  
E. coli OP50-pBAD, lyophilized 1
Cholesterol solution, 200 µl 1
Ampicillin, lyophilized, 30 mg 1
Store at room temperature  
NGM Lite Agar, 11 g 1
Agar, 4 g 1
2.5 M NaCl, 0.5 ml 1
C. elegans wash buffer (10x), 30 ml 1
Microcentrifuge tubes, 2.0 ml 60
Petri dishes, 60 mm 60
Disposable plastic transfer pipets (DPTPs) 50
Curriculum, (including Teacher's Guide, Student Manual, and graphic Quick Guide) 1
Redemption instructions for shipment of C. elegans 1
C. elegans, wild-type, 1 ml
C. elegans, mutant, 1 ml
 
Note: The wild-type and mutant worms are shipped on dry ice and must be kept on dry ice or at –70°C until ready to plate. The kit will be shipped prior to the shipment of worms to allow for preparation of NGM Lite agar plates. Wild-type and mutant C. elegans will be shipped overnight at date specified by customer.
Key Kit Features
  • Aligns with AP Biology Big Ideas 2, 3, and 4
  • Get hands-on experience with a model eukaryotic organism
  • Observe and study the life cycle of C. elegans
  • Utilize microscope skills
  • Learn about genetics and its effect on behavior
  • Student activities complete in two 45-minute lab sessions
C. elegans Videos

OpenWorm Resource



Learn about C. elegans' life cycle, their feeding habits, their neurological structure, and more.

C. elegans Movement



See C. elegans in motion and observe their movement patterns.

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The C. elegans Behavior Kit allows students to work with and learn about C. elegans, one of the most studied model organisms.

Key Features and Benefits

With this kit, students are able to:

  • Get hands-on experience with a model eukaryotic organism
  • Observe and study the life cycle of C. elegans
  • Utilize their microscope skills
  • Learn how to subculture
  • Learn about genetics and its effect on behavior
  • Discover the connection between learning, the daf-18 gene, and the AIY and ASE neurons

Background Information

C. elegans was the first multicellular organism to have its complete genome sequenced. The Nobel Prize in Physiology or Medicine was awarded to C. elegans researchers in 2002 (genetics of organ development and apoptosis), 2006 (RNA interference), and 2008 (GFP expression), emphasizing the importance of research on this model organism. This microscopic nematode is ideal for students to learn about subculturing so that they can observe the life cycle and different stages of development of the worms.

Following life cycle observation, students will get the chance to understand how C. elegans can learn to associate salt with their food by monitoring chemotaxis. When wild-type C. elegans are fed food with salt they learn to associate their food with the salt. C. elegans are then able to sense salt in their environment and will chemotax towards the salt in search of food. A mutant affected in the daf-18 gene (and subsequently the AIY and ASE neurons) results in a worm that is not able to learn to associate the salt with the food. While the mutant C. elegans are able to chemotax, their inability to associate their food to a salty environment results in no migration towards salt in a chemotaxis assay.

Note: The wild-type and mutant worms are shipped on dry ice and must be kept on dry ice or at –70°C until ready to plate. Allow for preparation of NGM Lite agar plates (reagents ordered separately) prior to ordering the worms. Wild-type and mutant C. elegans will be shipped overnight at a date specified by the customer.

Download our interactive calculator for setting up your instructor's advance lab preparation and for scheduling shipment of the C. elegans.

Kit Contents Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
Store at –20°C  
E. coli OP50-pBAD, lyophilized 1
Cholesterol solution, 200 µl 1
Ampicillin, lyophilized, 30 mg 1
Store at room temperature  
NGM Lite agar, 11 g 1
Agar, 4 g 1
2.5 M NaCl, 0.5 ml 1
C. elegans wash buffer (10x), 30 ml 1
Microcentrifuge tubes, 2.0 ml 60
Petri dishes, 60 mm, sterile 60
Disposable plastic transfer pipets (DPTPs) 50
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
C. elegans shipment redemption instructions 1
C. elegans, wild-type, 1 ml 1
C. elegans, mutant, 1 ml 1
Notes The wild-type and mutant worms are shipped on dry ice and must be kept on dry ice or at –70°C until ready to plate. The kit will be shipped prior to the shipment of worms to allow for preparation of NGM Lite agar plates. Wild-type and mutant C. elegans will be shipped overnight at date specified by customer.
Number Description Options
6673 Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories Click to download
6672 Map of AP-Aligned Bio-Rad Kits with Essential Knowledge Click to download
6605 Map of AP-Aligned Bio-Rad Kits with Learning Objectives Click to download
10040890 C. elegans Behavior Kit Chi Square Analysis Supplement, Rev A Click to download
10041144 C. elegans Behavior Kit Neurology Supplement, Rev A Click to download
10030940 Instruction Manual, Biotechnology Explorer™ C. elegans Behavior Kit, Rev B Please login to download this document
10033926 Biotechnology Explorer™ C. elegans Behavior Kit Planning Guide, Rev A Click to download
D102454 Bio-Rad Explorer™ C. elegans Behavior Kit Quick Guide, Rev B Click to download
10040011 Instruction Manual, Biotechnology Explorer™ C. elegans Behavior Kit, Bioinformatics Supplement, Rev A Please login to download this document

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