Biotinylated Standards

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Overview

Discontinued Products for Biotinylated Standards
Discontinued Biotinylated SDS-PAGE Standards 
Recommended Precision Plus Protein™ WesternC™ Standards 
Item # Product Description Item #
Product Description
161-0306 Low Range Standards
161-0376 
WesternC™ Standards
161-0307 Low Range Kit with HRP
161-0385 
WesternC™ Pack (Standard with HRP)
161-0308 Low Range Kit with AP
161-0376 + 161-0382
WesternC™ Standard and AP conjugate
161-0311 High Range Standards
161-0376 
WesternC™ Standards
161-0312 High Range Kit with HRP
161-0385 
WesternC™ Pack (Standard with HRP)
161-0313 High Range Kit with AP
161-0376 + 161-0382
WesternC™ Standard and AP conjugate
161-0319 Broad Range Standards
161-0376 
WesternC™ Standards
161-0321 Broad Range Kit with HRP
161-0385 
WesternC™ Pack (Standard with HRP)
161-0322 Broad Range Kit with AP
161-0376 + 161-0382
WesternC™ Standard and AP conjugate

Broad Range Biotinylated Standards
Broad Range Biotinylated SDS-PAGE Standards

Broad range biotinylated SDS-PAGE standards blotted to nitrocellulose and detected with avidin-AP.

This product is discontinued. Our recommended product is Precision Plus Protein™ WesternC™ Standards (161-0376) with Precision Protein™ StrepTactin-AP Conjugate (161-0382).

Constituent Proteins of Biotinylated SDS-Page Standards
Constituent Proteins of Biotinylated SDS-Page Standards
Constituent Proteins of Biotinylated SDS-Page Standards
    Type of Standard
Protein MW (kD) Broad Low High
Myosin 200.0  
β-Galactosidase 116.3  
Phosphorylase b 97.4
Bovine serum albumin (BSA) 66.2
Ovalbumin 45.0
Carbonic anhydrase 31.0  
Trypsin inhinitor 21.5  
Lysozyme 14.4  
Apoprotinin 6.5    
Biotinylated Standards

Biotinylated SDS-PAGE standards allow accurate MW determination directly on western blots, and are available in high, low and broad MW ranges.

Detection of biotinylated standards. Steps beyond the procedure for detecting sample proteins are required. Following transfer and addition of antigen-specific primary antibody, avidin-HRP or avidin-AP is added to the conjugated secondary antibody solution and applied to the membrane. Avidin binds to the biotinylated standards while the secondary antibody binds to the primary antibody. The standards and the protein of interest are detected simultaneously using the appropriate color development reagent system.

Bio-Rad’s natural biotinylated SDS-PAGE standards were designed to allow accurate molecular weight determination directly on western blots. These products are discontinued and have been replaced with our recombinant Precision Plus Protein Standards, which offer reproducible migration patterns and exception lot-to-lot molecular weight consistency.

MW size range High range: 200–45 kD
Broad range: 200–6.5 kD
Low range: 97.4–14.4kD
Stain type Biotinylated
Volume 250 µl
Shelf life 12 months
Streptavidin-AP

170-3554
0.5 ml, streptavidin alkaline phosphatase conjugate

List Price:   $73.00
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Avidin-HRP

170-6528
2 ml, HRP-Avidin conjugate for detection of biotinylated SDS-PAGE standards

List Price:   $107.00
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Avidin-AP

170-6533
1 ml, blotting-grade alkaline phosphatase (AP) avidin conjugate

List Price:   $117.00
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Number Description Options
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download [ Add to Cart (Free) ]
2414 The Little Book of Standards, Rev D Click to download [ Add to Cart (Free) ]
3118 Protein Standards for Electrophoresis and Blotting Brochure, Rev D Click to download [ Add to Cart (Free) ]
2998 Protein Standards Application Guide, Rev G Click to download [ Add to Cart (Free) ]
5561 Precision Plus Protein WesternC Standards, Rev E Click to download [ Add to Cart (Free) ]
LIT395 Instruction Manual, Biotinylated Standards, Low, High, and Broad Range, Rev D Click to download
M4006050 Instruction Manual, Biotinylated Standards Kit, Rev C Click to download