Biotinylated Protein Standards

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Biotinylated Standards

Overview

Biotinylated Standards

Biotinylated SDS-PAGE standards allow accurate MW determination directly on western blots, and are available in high, low and broad MW ranges.

Detection of biotinylated standards. Steps beyond the procedure for detecting sample proteins are required. Following transfer and addition of antigen-specific primary antibody, avidin-HRP or avidin-AP is added to the conjugated secondary antibody solution and applied to the membrane. Avidin binds to the biotinylated standards while the secondary antibody binds to the primary antibody. The standards and the protein of interest are detected simultaneously using the appropriate color development reagent system.

Constituent Proteins of Biotinylated SDS-Page Standards
Constituent Proteins of Biotinylated SDS-Page Standards
Constituent Proteins of Biotinylated SDS-Page Standards
    Type of Standard
Protein MW (kD) Broad Low High
Myosin 200.0  
β-Galactosidase 116.3  
Phosphorylase b 97.4
Bovine serum albumin (BSA) 66.2
Ovalbumin 45.0
Carbonic anhydrase 31.0  
Trypsin inhinitor 21.5  
Lysozyme 14.4  
Apoprotinin 6.5    
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Discontinued Product
Biotinylated Natural SDS-PAGE Standards have been discontinued. Precision Plus Protein™ WesternC™ Standards are the recommended replacement products. These prestained, Strep-tagged recombinant protein standards enable both fluorescent visualization on gels and colorimetric or chemiluminescent western blot detection with StrepTactin-AP or StrepTactin-HRP Conjugate.

Recombinant protein standards offer reproducible migration patterns and exceptional lot-to-lot molecular weight consistency, and are ideal for western blotting. See the Protein Ladders and Standards page to select the best protein standard for your application.

 

Replacement Products for Biotinylated Natural Protein Standards

Discontinued Biotinylated Protein Standard Recommended  Replacment Protein Standard Catalog Number
Low Range Standards (1610306) WesternC Standards  1610376
High Range Standards (1610311)
Broad Range Standards (1610319)
Low Range Kit with AP (1610308) WesternC Standards
+ StrepTactin-AP Conjugate
1610376
1610381
High Range Kit with AP (1610313)
Broad Range Kit with AP (1610322)
Low Range Kit with HRP (1610307) WesternC Standards with StrepTactin-HRP Conjugate 1610385
High Range Kit with HRP (1610312)
Broad Range Kit with HRP (1610321)
MW size range High range: 200–45 kD
Broad range: 200–6.5 kD
Low range: 97.4–14.4kD
Stain type Biotinylated
Volume 250 µl
Shelf life 12 months
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Streptavidin-AP

1703554
0.5 ml, streptavidin alkaline phosphatase conjugate

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Avidin-HRP

1706528
2 ml, HRP-Avidin conjugate for detection of biotinylated SDS-PAGE standards

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Avidin-AP

1706533
1 ml, blotting-grade alkaline phosphatase (AP) avidin conjugate

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Number Description Options
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download
2414 The Little Book of Standards, Rev D Click to download
3118 Protein Standards for Electrophoresis and Blotting Brochure, Rev D Click to download
2998 Protein Standards Application Guide, Rev G Click to download [ Add to Cart (Free) ]
5561 Precision Plus Protein™ WesternC™ Standards, Rev E Click to download
LIT395 Instruction Manual, Biotinylated Standards, Low, High, and Broad Range, Rev D Click to download
M4006050 Instruction Manual, Biotinylated Standards Kit, Rev C Click to download

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