Bio-Spin® Columns with Bio-Gel® P-6

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Overview

Specifications
  Bio-Spin 6 Micro Bio-Spin 6 Bio-Spin 30 Micro Bio-Spin 30
Packed support Special grade Bio-Gel P-6 gel Special grade Bio-Gel P-6 gel Special-grade Bio-Gel P-30 gel Special-grade Bio-Gel P-30 gel
Equilibration buffer SSC-buffer* 10 mMTris, pH 7.4 or SSC buffer* SSC buffer* 10 mM Tris, pH 7.4 or SSC buffer*
Applications Desalting and buffer exchange Desalting and buffer exchange Desalting; nucleotide and small molecule removal DNA sequencing reaction mixtures (Tris), and small molecule removal
Bed volume 1.1 ml 0.7 ml 1.1 ml 0.7 ml
Retention and recovery 90% recovery of 20 bases or bp, 99% retention of salts 90% recovery of 20 bases or bp, 99% retention of salts 95% recovery of 22 bases or bp, 98% retention of ddNTPs 95% recovery of 22% bases or bp, 98% retention of ddNTPs
MW exclusion limit, globular proteins 6,000 6,000 40,000 40,000
Sample volume 50–100 µl 10–75 µl 50–100 µl 10–75 µl
Centrifuge type Swinging bucket Microcentrifuge Swinging bucket Microcentrifuge
Autoclavability Yes Yes Yes Yes


*150 mM NaCl, 17.5 mM sodium citrate, pH 7.0

Prepacked Gravity and Spin Columns
  Bio-Spin 6 Micro Bio-Spin 6 Bio-Spin 30 Micro Bio-Spin 30 PCR Kleen
Packed support Special grade
Bio-Gel P-6 gel
Special grade
Bio-Gel P-6 gel
Special grade
Bio-Gel P-30 gel
Special grade
Bio-Gel P-30 gel
Special grade
Size exclusion gel
Equilibration buffer 10 mM Tris, pH 7.4 or SSC buffer* 10 mM Tris, pH 7.4 or SSC buffer* 10 mM Tris, pH 7.4 or SSC buffer* 10 mM Tris, pH 7.4 or SSC buffer* 10 mM Tris, 1 mM EDTA, pH 7.0
Applications Desalting of oligonucleotides >20 bases - - -
Labeling reactions:
removal of unincorporated nucleotides >20 bases or bp from DNA
- - -
Removal of primers and primer-dimers from PCR products >200 bp - - - -
Buffer exchange
(restriction fragments, PCR products, enzyme reactions, sequencing templates.)
- -
DNA sequencing
reaction mixture cleanup**
- - -
Riboprobe cleanup*** - - - -
Desalting of antibody, enzyme, and protein solutions - -
Purification of proteins of moleular weight >6,000 - - -
Purification of proteins of molecular weight >40,000 - - -
Bed Volume 1.1 ml 0.7 ml 1.1 ml 0.7 ml 0.6 ml
Retention and recovery 90% recovery of 20 bases or bp, 99% retention of salts 90% recovery of 20 bases or bp, 99% retention of salts 95% recovery of 22 bases or bp, 98% retention of ddNTPs 95% recovery of 22 bases or bp, 98% retention of ddNTPs 85% recovery of ≥700bp, 95% retention of primers and primer-dimers
Molecular weight exclusion limit, globular proteins 6,000 6,000 40,000 40,000 8,000,000
Sample volume 50–100 µl 10–75 µl 50–100 µl 10–75 µl 25–100 µl
Centrifuge type Swinging bucket Microcentrifuge Swinging bucket Microcentrifuge Microcentrifuge
Autoclavability Yes Yes Yes Yes Yes

* 150 mM NaCl, 17.5 mM sodium citrate, pH 7.0
** In Tris buffer.
*** In RNase-free Tris buffer.

PCR Product and DNA Fragment Purification Guide

Bio-Rad offers a variety of pre-PCR and post-PCR sample preparation and cleanup columns and prepacked columns; many are also suitable for DNA fragment purification.

  Freeze 'N Squeeze™ DNA
Gel Extraction Spin Columns
Micro Bio-Spin® and Bio-Spin Columns PCR Kleen™
Spin Columns
Application Recovery of DNA fragments (50 bp–23 kb) purified from TAE- or TBE-buffered agarose gels Dye terminator and label cleanup from sequencing reactions; buffer exchange; desalting Removal of PCR products >200 bp from other reaction mixture components
Starting material ≤700 mg gel slice 10–100 µl of sequencing reation mixture 25–100 µl of PCR or other enzyme reaction
Format Spin-column extraction of frozen-thawed gel slices Prepacked size exclusion spin column Prepacked size exclusion spin column
Recovery 4–23 kb: >80% recovery;
0.5–4 kb:>70% recovery;
50–500 bp:>50% recovery
>95% of linear DNA >22 bases or bp; 99% retention of unincorporated dye terminators 50–80%
Time required <10 min <10 min <4 min



Bio-Spin and Micro Bio-Spin columns clean up DNA and protein samples quickly. Filled with specially sized Bio-Gel P gels, Bio-Spin and Micro Bio-Spin columns are shipped fully hydrated in Tris or SSC buffer and are ready to use. These products effectively clean up and remove salts, nucleotides, dye terminators, and small molecules from DNA, RNA, and protein samples.

Features include:

  • Convenience of prepacked, prehydrated gel support
  • Rapid sample cleanup with a 4 minute microcentrifuge spin
  • Efficient (98%) removal of ddNTPs and dye terminators
  • Improved yield — 95% recovery of DNA >22 bp
  • Sample loads from 10 to 100 µl
  • Safe riboprobe preparation with RNase-free Micro Bio-Spin P-30 Tris spin columns
Bio-Spin® P-6 Gel Columns, Tris Buffer

732-6227
Pkg of 25, prepacked spin columns with Bio-Gel® P-6 gel in Tris buffer, sample volume 50–100 μl, 6,000 MW limit, includes 50 collection tubes

List Price:   $120.00
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Bio-Spin® P-6 Gel Columns, Tris Buffer

732-6228
Pkg of 100, prepacked spin columns with Bio-Gel® P-6 in Tris buffer, sample volume 50–100 μl, 6,000 MW limit, includes 200 collection tubes

List Price:   $419.00
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Bio-Spin® P-6 Gel Columns, SSC Buffer

732-6002
Pkg of 25, prepacked spin columns with Bio-Gel® P-6 in SSC, sample volume 50–100 μl, 6,000 MW limit, includes 50 collection tubes

List Price:   $143.00
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Bio-Spin® P-6 Gel Columns, SSC Buffer

732-6213
Pkg of 1,000, prepacked spin columns with Bio-Gel® P-6 in SSC, sample volume 50–100 μl, 6,000 MW limit, includes 2,000 collection tubes

List Price:   $3,519.00
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Number Description Options
2320 Bio-Spin and Micro Bio-Spin Column Product Information Sheet, Rev A Click to download
LIT507 Instruction Manual, Bio-Spin 6 and 30 Columns, Rev G Click to download