Bio-Scale™ Mini UNOsphere SUPrA™ Affinity Cartridges

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Overview

Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 732–4120 732–4122 732–4124
UNOsphere™ Q NOsphereQ Strong anion exchange –N+(CH3)3 180 mg BSA 732–4100 732–4102 732–4104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 732–4110 732–4112 732–4114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 732–4140 732–4142 732–4144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 732–4130 732–4132 732–4134
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

732–4632 732–4634
Antibody Purification
Protein A Affi-Prep® protein A Affinity Recombinant protein A 30 mg/ml human IgG
732–4200, 1 x 1 ml 732–4201, 5 x 1 ml 732–4202, 1 x 5 ml
Protein A Affi-Prep® protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
732–4600 732–4602
CHT™ Type I CHT ceramic
hydroxyapatite Type I
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG
732–4322 732–4324
CHT Type II CHT ceramic
hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG
732–4332 732–4334
Affinity-Tagged Protein Purification
IMAC** Nuvia™ IMAC Affinity Nitrilotriacetic acid ≥40 mg recombinant
Histidine-tagged protein
780–0811 780–0812
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
732–4620 732–4622 732–4624
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
732–4610 732–4612 732–4614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
732–4620 732–4622 732–4624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
732–4642 732–4644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5 ml 5 x 5 ml 5 x 10 ml 1 x 50 ml 5 x 50 ml
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV*** 732–
4502
732–
4504
732–
5304
732–
5312
732–
5314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.

** IMAC and GST cartridges are designed for use with the Profinia protein purification system.

*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

Bio-Scale Mini Cartridge Specifications
Composition Highly cross-linked polymer
Particle Size Range 53–61 µm
Ligand Recombinant Protein A
Coupling Chemistry Epoxy
Working pH range 3–11
Dynamic Binding Capacity* 30 ± 3 mg/ml at 150 cm/hr
25 ± 2 mg/ml at 300 cm/hr
20 ± 2 mg/ml 450 cm/hr
(Minimum spec: 20 mg/ml at 300 cm/hr)
Chemical Stability 10 mM hydrochloric acid
6 M guanidine hydrochloride
0.1 M arginine (pH 2.8)
0.1 M citrate (pH 2.8)
0.1 M glycine (pH 2.8)
Cleaning-In-Place (CIP) Solutions 6 M guanidine hydrochloride
10 mM hydrochloric acid
0.1 M sodium hydroxide
1 M acetic acid/20% ethanol
Recommended mobile phase velocity range 100–600 cm/hr
Temperature stability 4–40°C
Delivery conditions 50% slurry in 20% ethanol
Storage conditions 4–8°C

*   10% breakthrough capacity determined with 1.0 mg/ml polyclonal human IgG in 1.1 x 10 cm column.
** No significant change in chromatographic performance

Monoclonal Antibody Workflow

Workflow for mAb purification with UNOsphere SUPrA affinity, UNOsphere Q, and CHT Type 1 media.

To read more click here

Flow Performance of UNOsphere SUPrA Media

Narrow elution profile of antibody on UNOsphere SUPrA media.

UNOsphere SUPrA affinity chromatography support is based on recombinant protein A and is designed for process-scale purification of monoclonal antibodies. The protein A ligand is produced in E. coli without the use of material of animal origin.

Bio-Scale Mini cartridges have a polypropylene double-wall construction* that provides extra strength and allows reliable runs up to 45 psi with the aqueous buffers most commonly used for protein separations. Bio-Scale Mini cartridges are ideal for low-pressure chromatography applications. Luer fittings make connections simple; adapters are available to allow connection to all types of chromatography systems.

Bio-Scale Mini UNOsphere SUPrA cartridges are available in 1 ml and 5 ml sizes. These cartridges are prepacked with UNOsphere SUPrA affinity media and are ideal for method scouting and small-scale purifications.

* U.S. Patent 7,208,087.

Bio-Scale™ Mini UNOsphere™ Rapid S cartridges, 5 x 1ml

732-4400
Pkg of 5, 1 ml, prepacked, UNOsphere rapid S cation exchange media cartridge

List Price:   $189.00
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Bio-Scale™ Mini UNOsphere™ Rapid S cartridges, 1 x 5 ml

732-4401
5 ml, prepacked, UNOsphere rapid S cation exchange media cartridge

List Price:   $133.00
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Bio-Scale™ Mini UNOsphere™ Rapid S cartridges, 5 x 5 ml

732-4402
Pkg of 5, 5 ml, prepacked, UNOsphere rapid S cation exchange media cartridge

List Price:   $461.00
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Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 1 x 1 ml

732-4200
1 ml, prepacked, UNOsphere SUPrA affinity media cartridge

List Price:   $143.00
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Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 5 x 1 ml

732-4201
Pkg of 5, 1 ml, prepacked, UNOsphere SUPrA affinity media cartridge

List Price:   $620.00
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Bio-Scale Mini UNOsphere SUPrA affinity chromatography cartridges, 1 x 5 ml

732-4202
5 ml, prepacked, UNOsphere SUPrA affinity media cartridge

List Price:   $527.00
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Number Description Options
6498 Lab-Scale Protein Purification Column and Cartridge Selection Guide, Rev B Click to download [ Add to Cart (Free) ]
5729 UNOsphere SUPrA Affinity Chromatography Media Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
5728 Monoclonal Antibody Purification Using UNOsphere SUPrA Media, Rev A Click to download [ Add to Cart (Free) ]
10014997 Instruction Manual, Bio-Scale Mini UNOsphere SUPrA cartridges, 1 and 5 ml, Rev A Click to download
10014430 Instruction Manual, UNOsphere SUPrA Affinity Chromatography Media, Rev A Click to download
5326 Chromatography Column Connection Guide, Rev A Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download